Native-born Research Articles

Asian American Surgery: A Short History of Immigration, Naturalization, and Refugee Laws and Policies that Brought Asians to America.

Chinese immigration in America in the mid-nineteenth century incited violence and governmental sanctions to restrict Asian immigration and reject citizenship rights. In 1870, Chinese strikebreakers in Massachusetts ignited nationwide demonstrations against Chinese immigration, which ended altogether with the Chinese Exclusion Act (1882). Japanese schoolchildren in San Francisco public schools sparked rioting in 1907, leading Japan to unilaterally limit emigration to the United States (Gentlemen's Agreement, 1908). Congress responded to anti-Punjabi riots (Bellingham, WA, 1907) by refusing naturalization rights to Asians (Barred Zone Act, 1917), policies supported by the Supreme Court (Ozawa, 1922; Thind, 1923). All immigration from Asia was soon prohibited (Johnson-Reed Act, 1924). Anti-Asian measures peaked in 1942 with imprisonment of Japanese in concentration camps, including those with birthright citizenship. Anti-Asian policies unwound in the last half of the 20th century, culminating in sweeping changes in US immunization policy. Naturalization rights were granted to wartime allies against Japan (China, 1943; the Philippines and India, 1946) and the Japanese themselves (1952). Asian women marrying American servicemen were allowed entry into the country (1945, 1950). Prohibitions against Asian immigration were lifted in 1965 (Hart-Celler Act), with preference for those trained in science, technology, and medicine. The refugee crisis that followed the fall of Saigon (1975) drove humanitarian policies that brought evacuees from Southeast Asia and others from throughout the world seeking freedom from persecution (Refugee Act, 1980). Hundreds of thousands from China, South Asia, the Philippines, and Southeast Asia thus settled in the United States, changing American society and medicine.

Canadian Club: Birthright Citizenship and National Belonging by Lois Harder

<i>Canadian Club: Birthright Citizenship and National Belonging</i> by Lois Harder

The Distinctive Biology and Characteristics of the Bare-Nosed Wombat (Vombatus ursinus).

The bare-nosed wombat is an iconic Australian fauna with remarkable biological characteristics and mythology. This solitary, muscular, fossorial, herbivorous marsupial from southeast Australia has continent and continental island subspeciation. Vombatiformes also contains hairy-nosed wombats (Lasiorhinus spp.); koala (Phascolarctos cinereus); and extinct megafauna, Phascolonus gigas (giant wombat), Diprotodon, and Thylacoleo (marsupial lion). Culturally important to Aboriginal people, bare-nosed wombats engineer ecosystems through digging, grazing, and defecation. Olfaction and cubic fecal aggregations appear critical for communication, including identity, courtship, and mating. Though among the largest fossorial herbivores, they have a nutrient-poor diet, a home range up to an order of magnitude smaller than expected, and a metabolism among the lowest extreme for mammals >10 kg. Metabolic depression may confer advantages over resource competitors and fossorial lifestyle protection from predators, fires, and climatic extremes. Bare-nosed wombats are loved and persecuted by European colonists. Recent population increases may reflect softening attitudes toward, and greater protections of, bare-nosed wombats.

Unmade in America: The gendered politics of birth tourism in the United States

This paper interrogates the discursive production of the “birth tourist” and associated opposition to jus soli birthright citizenship in the United States. I trace the intersecting axes of heteropatriarchy and racism that underpin history of opposition to jus soli citizenship, and position contemporary media and political attention to “birth tourism” as an extension of those axes. Through a critical discourse analysis of popular media coverage and public commentators, I position the “birth tourism” category within a broader national discourse of white supremacy, before linking this discursive production to national-level regulatory efforts. Interrogating the “birth tourist” categorization as a discursive tool of white, patriarchal ethnonationalism, I advance the study of political geographies of fertility and argue that contradictions embedded within the “birth tourism” discourse ultimately work to fuel fears of white supremacy's collapse. Keywords: birth tourism, birthright citizenship, white supremacy, intersectionality, feminist geopolitics.

Efficacy of a synthetic peptide Chlamydia pecorum major outer membrane protein vaccine in a wild koala (Phascolarctos cinereus) population

Chlamydiosis is a significant disease affecting Eastern Australian koala (Phascolarctos cinereus) populations, impacting individual animal welfare and fecundity and therefore influencing population dynamics. The aim of this study was to investigate the effect of a synthetic peptide vaccine based on 4 components of the Chlamydia pecorum major outer membrane protein (MOMP), over an 18-month period in a koala population severely impacted by chlamydiosis. Wild koalas were recruited into a vaccination or a placebo treatment group on a random allocation, then followed through a period of 18 months, with recapture at 6 monthly intervals. Vaccination did not alter clinical disease expression or chlamydial shedding from the ocular or urogenital sites. Vaccination did not stimulate a significant plasma anti-MOMP IgG response, when compared to the placebo group. There was no significant effect of vaccination on IFN-γ and IL-17A mRNA expression of peripheral blood lymphocytes when stimulated with rMOMP. We have demonstrated that a synthetic peptide vaccination against chlamydiosis is not an effective management tool in a koala population with a high prevalence of C. pecorum infection and related disease. The lack of antigenic response found in this study suggests that further research utilising a larger, full-length antigen is an avenue worth investigation if we are to consider vaccination as a part of a management strategy in diseased koala populations.

Health Assessments of Koalas after Wildfire: A Temporal Comparison of Rehabilitated and Non-Rescued Resident Individuals.

Many koalas (Phascolarctos cinereus) required rehabilitation after the 2019/20 Australian megafires. Little is known about how the post-release health of rehabilitated koalas compares to non-rescued resident koalas. We evaluated health parameters in rehabilitated koalas and resident koalas in burnt and unburnt habitat in southern New South Wales, Australia. Health checks were undertaken within six weeks of fire (rehabilitated group), 5-9 months post-fire and 12-16 months post-fire. Body condition improved significantly over time in rehabilitated koalas, with similar condition between all groups at 12-16 months. Rehabilitated koalas therefore gained body condition at similar rates to koalas who remained and survived in the wild. The prevalence of Chlamydia pecorum was also similar between groups and timepoints, suggesting wildfire and rehabilitation did not exacerbate disease in this population. While there was some variation in measured serum biochemistry and haematology parameters between groups and timepoints, most were within normal reference ranges. Our findings show that koalas were generally healthy at the time of release and when recaptured nine months later. Landscapes in the Monaro region exhibiting a mosaic of burn severity can support koalas, and rehabilitated koala health is not compromised by returning them to burnt habitats 4-6 months post-fire.

Trends in the rescue and fate of koalas in New South Wales (1973-2020), with a focus on disease and trauma

ABSTRACT In line with the objectives of both the 2022 National Recovery Plan for the Koala Phascolarctos cinereus and 2022 NSW Koala Strategy, our study aimed to draw on the long-term records of koala rescue in NSW from 1973 to 2020 to describe trends in admissions across NSW. A total of 18,039 koala admission records were collated from 1973 to 2020. Koalas were rescued due to disease, motor vehicle collision, dog attacks, unsuitable environment, bushfire, orphaning, and other reasons. However, the reasons for rescue were not evenly distributed among the rehabilitation groups. Across all rehabilitation groups and all years (1973-2020), the release rate of all admitted koalas was 47.6%. The NSW Koala Strategy identifies an action of “supporting koala rehabilitators” (Pillar 3 Improving the safety and health of koalas), and strong partnerships, including with koala rehabilitators, are “critical to achieving success”. Our study has confirmed that strong partnerships are possible and identified the prevalence of disease and trauma in rescued koalas. While it is not possible to assess the scale of the threats from disease and trauma to koala populations from rehabilitation records alone, we can use the trends in admissions to inform the efficient distribution of resources to rescue and rehabilitation efforts.

The koala (Phascolarctos cinereus) prostate: a comprehensive histological and immunohistochemical investigation.

The prostate of the koala (Phascolarctos cinereus), and of marsupials more generally, is the primary contributor of seminal fluid, yet comparatively little is known about its microanatomy or biochemistry. This study explored evidence of parenchymal segmentation of the koala prostate. The prostate of three sexually mature koalas were processed for histopathology, histochemistry (Masson's trichrome, Alcian Blue, periodic acid Schiff staining), and immunohistochemistry using basal (tumor protein 63, cytokeratin 14) and luminal (cytokeratin 8/18, prostate specific antigen, androgen receptor) markers. Results confirmed clear segmentation of the koala prostate into three zones, anterior, central, and posterior, characterized by differences in the proportion of glandular tissue, as well as the thickness of collagen fibers; there were also distinct differences in the secretions produced in each zone. Based on immunohistochemistry, the koala prostate showed evidence of both basal proliferative and luminal secretory cells. The ratio of cell types varied across the three segments, with the central segment housing the highest density of basal cells. Globular bodies produced in the anterior zone were shown to possess the same markers as those described for human prostasomes. This study is the first to comprehensively document the marsupial prostate in terms of microanatomy and corresponding immunohistochemistry. While further biochemical analysis, such as proteomics of each segment will better define the relative functions of each tissue, the data presented here are consistent with the hypothesis that the koala prostate potentially represents an example of an ontological stage in the evolutionary differentiation of male eutherian accessory glands.

Slaves, Migrants and the Stateless

Proponents of barring the children of undocumented immigrants from birthright citizenship allege that the United States (‘US’) Constitution’s 14th Amendment was intended to give full citizenship to former slaves and their progeny, and not to benefit the children of foreign-born people. A real-world example that illustrates the dangers of so restricting birthright citizenship is the Dominican Republic, where legal measures have already excluded the children of out-of-status immigrants (who are mostly of Haitian ancestry) from eligibility for birthright citizenship. The effect of this has not been ethnically cleansing Haitian descendants from the Dominican Republic so much as confining them within the country as a stateless underclass of people. The Dominican case therefore shows that US opponents of birthright citizenship for the children of out-of-status non-citizens must answer to the danger that their proposal would create a legally approved hereditary underclass on US soil, more than a century after the abolition of chattel slavery.

Influence of scat ageing on the gut microbiome: how old is too old?

BackgroundThe study of the host-microbiome by the collection of non-invasive samples has the potential to become a powerful tool for conservation monitoring and surveillance of wildlife. However, multiple factors can bias the quality of data recovered from scats, particularly when field-collected samples are used given that the time of defecation is unknown. Previous studies using scats have shown that the impact of aerobic exposure on the microbial composition is species-specific, leading to different rates of change in microbial communities. However, the impact that this aging process has on the relationship between the bacterial and fungal composition has yet to be explored. In this study, we measured the effects of time post-defecation on bacterial and fungal compositions in a controlled experiment using scat samples from the endangered koala (Phascolarctos cinereus).ResultsWe found that the bacterial composition remained stable through the scat aging process, while the fungal composition did not. The absence of an increase in facultative anaerobes and the stable population of obligate anaerobic bacteria were likely due to our sampling from the inner portion of the scat. We report a cluster of fungal taxa that colonises scats after defecation which can dilute the genetic material from the autochthonous mycoflora and inhibit recovery.ConclusionWe emphasize the need to preserve the integrity of scat samples collected in the wild and combat the effects of time and provide strategies for doing so.

Inheritance Rights to Freehold Land for Children with Dual Citizenship Outside of Mixed Marriage

Inheritance is a legal consequence of a legal event in the form of death, which is explained in Article 830 of the Criminal Code. It is also explained that every person who has the right to inherit without distinction of male or female, or who was first born with the same amount for each heir, is stated in Article 852 Paragraphs (1) and (2) of the Criminal Code. In addition, if the child obtains the status of an Indonesian citizen from the Ius Sanguinis principle and obtains the status of a foreigner from the Ius Soli principle, it does not cause the child to become a dual nationality child because the Citizenship Law does not recognize the existence of dual citizenship as stated in the general explanation. However, the Citizenship Law provides an exception for children with dual citizenship. The granting of an exception to dual citizenship lasts until the child has chosen a nationality. The election of citizenship is at the age of 18 years or has been married and an extension of time for 3 years based on Article 6 of the Citizenship Law. Dual citizenship status does not abolish the right to inherit children from both parents, but there are restrictions on the right to inherit in the form of property rights. Legal protection for children with dual citizenship status in their inheritance rights can be assisted in the management by a guardian. The purpose of this study is as one of the requirements for obtaining a Masters of Notary degree at the Postgraduate Notary Masters Study Program at Warmadewa University. This thesis research method uses normative legal research, which uses two types of approaches, namely the statutory approach, and the conceptual approach.

Performance of habitat offsets for species conservation in dynamic human‐modified landscapes

Abstract Biodiversity offsets are a popular policy tool for mitigating the impact of development on biodiversity, but the ecological success of offsets arise from complex interactions among socio‐economic, ecological and policy processes, making outcomes challenging to assess. Many offset policies use habitat surrogates to determine offset requirements, rather than using direct measures of impacted biota, and this can lead to poor outcomes for species. One potential solution to this is for offsets to be delivered by a public agency (agency‐led) rather than by developers (developer‐led). This is because agencies may be able to strategically choose offset sites that maximise outcomes for species (e.g. abundance), while there may be little reason for developers to act strategically in this way when offset requirements are based purely on habitat surrogates. Yet, the success of a strategic agency‐led approach is likely to depend on patterns of development and offset site availability. To examine this, we developed a novel integrated spatially explicit model of land‐use change, habitat, species abundance and offset regulation. We apply the model to the Queensland Government's Environmental Offsets Policy for koalas Phascolarctos cinereus in South East Queensland, Australia, and test how patterns of development and offset site availability influence the performance of agency‐led versus developer‐led offsets. When potential offset sites were plentiful, agency‐led offsets tended to outperform developer‐led offset delivery for maximising koala abundance while achieving similar or better outcomes for habitat area. Yet, when potential offset sites were rare, the relative performance of agency‐led offset was often poor, and offset requirements for habitat area were less likely to be met. Different spatial patterns of development had little effect on the relative performance of agency‐led versus developer‐led offsets. Our analysis shows that agency‐led offsets with strategic choices of offset sites can improve species' outcomes for habitat‐based offsets but can also risk failing to meet habitat area requirements when the availability of offset sites is low. Importantly, our integrated spatial model provides a holistic approach to assessing policy options for biodiversity offsets in dynamic human‐modified landscapes. Read the free Plain Language Summary for this article on the Journal blog.

LONEPINELLA SP. ISOLATED FROM WOUND INFECTIONS OF KOALAS.

We describe two cases of wound infections of koalas (Phascolarctos cinereus), one wild and one captive, in which Lonepinella-like organisms were involved. The wild adult koala was captured with bite wound injuries, as part of a koala population management program in Queensland, Australia. In both cases, there was evidence of physical trauma causing the initial wound. The captive koala suffered injury from the cage wire, and the wild koala had injuries suggestive of intermale fighting. Gram-negative bacteria isolated from both cases proved to be challenging to identify using routine diagnostic tests. The wound in the captive koala yielded a pure culture of an organism shown by whole genome sequence (WGS) analysis to be a member of the genus Lonepinella, but not a member of the only formally described species, L. koalarum. The wound of the wild koala yielded a mixed culture of Citrobacter koseri, Enterobacter cloacae and an organism shown by WGS analysis to be Lonepinella, but again not Lonepinella koalarum. Both cases were difficult to treat; the captive koala eventually had to have the phalanges amputated, and the wild koala required removal of the affected claw. The two Lonepinella isolates from these cases have a close relationship to an isolate from a human wound caused by a koala bite and may represent a novel species within the genus Lonepinella. Wound infections in koalas linked to Lonepinella have not been reported previously. Wildlife veterinarians need to be aware of the potential presence of Lonepinella-like organisms when dealing with wound infections in koalas, and the inability of commercial kits and systems to correctly identify the isolates.

Putative koala retrovirus-associated diseases in the Japanese captive koala (Phascolarctos cinereus) population. In Proceedings of the Second Koala Retrovirus Workshop, ed. D. E. Alquezar-Planas, D. P. Higgins, C. L. Singleton, and A. D. Greenwood

Japan began housing koalas (Phascolarctos cinereus) in 1984, increasing from six individuals in 1984 to a peak of 96 koalas in 1997. However, the number of koalas has almost halved since and as of 2020, 54 koalas remain in zoos in Japan. Although records of 330 koala deaths have been accumulated over 37 years, there have been no comprehensive reports on the relationship between the causes of death and koala retrovirus (KoRV) in the Japanese captive population. Based on the koala studbook updated by the Japanese Association of Zoos and Aquariums, we have investigated causes of death in the Japanese captive koala population. The most common cause of death was joeys falling. When combined with stunted joey growth, one-third of the koalas died within a year of birth. Deaths due to malignant neoplasms and opportunistic infections cannot be directly associated with KoRV infection because no test for KoRV had been performed before or during disease onset. It is suspected that KoRV may be associated with deaths due to the large number of cases of neoplasms, which accounted for 16.4% of all deaths.

Koala retrovirus infection and disease in South Australian koala (Phascolarctos cinereus) populations. In Proceedings of the Second Koala Retrovirus Workshop, ed. D. E. Alquezar-Planas, D. P. Higgins, C. L. Singleton, and A. D. Greenwood

Koala retrovirus (KoRV) infection, endogenous in all northern koalas (Phascolarctos cinereus), has been found to occur at lower, but increasing, prevalence in the Kangaroo Island and Mount Lofty Ranges koala populations in South Australia. Proviral and viral loads are also lower than in Queensland koalas, which may be due to exogenous spread of infection, or may be related to the variable presence of viral genes and fragmented expression that has been found in positive Mount Lofty Ranges koalas. However, high proviral loads and full expression across the KoRV genome in South Australian koalas has been found in individuals with neoplasia, particularly lymphoma, which can be as extensive and as severe as that observed in northern koalas. KoRV-A is the predominant subtype and no association with chlamydial status has been found except that high viral loads correlate with severity of chlamydiosis. Based on the complexity of KoRV infections in South Australian koalas, further research is needed to understand the differences in transmission and pathogenesis that occur.

Koala retrovirus genetic diversity and transmission: advice for breeders. In Proceedings of the Second Koala Retrovirus Workshop, ed. D. E. Alquezar-Planas, D. P. Higgins, C. L. Singleton, and A. D. Greenwood

The rapid spread of koala retrovirus (KoRV) across Australia and international zoo populations has necessitated appropriate control measures. Along with pathogenicity, the genetic diversity of the virus and how it transmits between animals also needs to be considered when deciding the most suitable measures. Next generation sequencing has become the gold standard approach for KoRV diversity studies due to the high sensitivity, accuracy, and throughput. This approach has identified a large proportion of known KoRV diversity and has provided a broader understanding of KoRV prevalence and abundance within koala (Phascolarctos cinereus) populations, specifically identifying individuals with low diversity. Recent evidence has demonstrated that exogenous KoRV transmits from mother to joey, likely through the ingestion of milk and/or pap, and that koalas are not likely to acquire additional KoRV subtypes/sequences later in life. This finding strongly indicates that breeding with KoRV negative or endogenous KoRV-A positive only females is the best chance at alleviating exogenous KoRV from koala populations worldwide. Captive breeders are therefore urged to determine the KoRV profile of all animals included in their breeding program through deep sequencing methods (where feasible) and use this to inform their future breeding regimes.

Endogenous and exogenous koala retrovirus patterns in wild koalas across Australia. In Proceedings of the Second Koala Retrovirus Workshop, ed. D. E. Alquezar-Planas, D. P. Higgins, C. L. Singleton, and A. D. Greenwood

Our understanding of koala retrovirus (KoRV) has advanced dramatically in recent years. Cross-sectional studies examining hundreds of wild koalas (Phascolarctos cinereus) from populations across their natural Australian range (Queensland–New South Wales–Victoria) have shed new light on KoRV abundance and diversity in the wild. A single strain of KoRV (the originally characterized Hanger strain from 2000) appears to be the dominant KoRV strain within koalas, endogenous in northern populations and the predominant exogenous strain in southern populations. Alongside this strain are potentially exogenous variants representing both intact and defective versions of some of the many recognized KoRV subtypes (KoRV-A to KoRV-M). The patterns of these may suggest a transition from endogenous KoRV in the north to exogenous KoRV in the south, occurring in southern New South Wales. They also highlight how actively the hypervariable region of the envelope gene of KoRV is diversifying, with fragmented koala populations across the country containing unique and distinctive KoRV proviral profiles. As more koala populations are examined with increasingly sensitive and specific genetic tools, our understanding of KoRV is poised to continue to evolve as quickly as the virus itself.

An overview of koala retrovirus epidemiology in Australia. In Proceedings of the Second Koala Retrovirus Workshop, ed. D. E. Alquezar-Planas, D. P. Higgins, C. L. Singleton, and A. D. Greenwood

Koala retrovirus (KoRV) epidemiology varies across koala (Phascolarctos cinereus) populations with distinct differences in viral prevalence, sequence diversity, and disease impact. Curiously the more genetically restricted southern populations are less impacted by KoRV with the virus not endogenized in its replication competent form in these animals. These southern animals do, however, have replication defective recKoRV variants in their genomes indicating historical exposure to KoRV and recKoRV. Whether southern animals are inherently resistant to KoRV infection and endogenization is not clear. It is also not clear whether the current regional epidemiological patterns will persist or whether exposure to animals with infectious KoRV or cross-breeding between different genetic populations will change the KoRV prevalence with time.

Incidence, trends, and significance of putative koala retrovirus-associated diseases in monitored wild koala populations in southeast Queensland. In Proceedings of the Second Koala Retrovirus Workshop, ed. D. E. Alquezar-Planas, D. P. Higgins, C. L. Singleton, and A. D. Greenwood

Research indicates that northern koalas (Phascolarctos cinereus) are ubiquitously infected with koala retrovirus (KoRV). There is increasing evidence linking KoRV with neoplasia and a range of disorders associated with immunodeficiency, conditions observed at high rates in captive colonies, and sick koalas presenting to wildlife hospitals. However, less is known about the occurrence of these putative KoRV-associated diseases in wild populations. We analysed health data collected at the veterinary examinations of 691 koalas inhabiting three monitored wild koala populations in southeast Queensland between 2013 and 2020. At initial presentation, neoplasia and AIDS-like syndrome were detected at a prevalence of 1.16% (8/691; 95% CI 0.5–2.19%). Longitudinal data from koalas recruited into the monitoring programmes and receiving one or more subsequent examination revealed an incidence rate of 3.5 cases/100 koalas/year (95% CI 2.35–4.9). These findings indicate that a relatively small proportion of the populations studied were affected by these putative KoRV-associated diseases. However, the impact on individuals was severe, with high associated mortality in the diseased cohort. Furthermore, northern koala populations endure multiple threats, suffering severe declines in recent decades. We propose that the significance of putative KoRV-associated diseases on these populations should be considered within this context and that further research into the interactions between KoRV and other drivers of decline is warranted.

Koalas showed limited behavioural response and no physiological response to drones

Drones have become a popular conservation tool especially when monitoring cryptic species or species inhabiting locations difficult to access. We developed a non-invasive methodology to measure heart rate in koalas (Phascolarctos cinereus) using a Fitbit and investigated the behavioural (vigilance behaviour) and physiological (heart rate and breathing rate) responses of captive koalas to drones. We showed for the first time that heart rate values in koalas can be accurately obtained with a Fitbit. Koalas responded to a drone flight conducted 15 m above their heads with a short-term increase in vigilance, but no change in heart rate or breathing rate. Our results suggest that drones may not have long-term detrimental effects on koalas’ fitness or energy demands and adds to the growing literature investigating animals’ responses to drones to help develop best practices for drone monitoring.