AbstractIn order to study the hydrocarbon generation (HCGE) characteristics of coal‐bearing basins, the coal‐measure source rocks of the Middle Jurassic–Lower Jurassic (MLJ) of the piedmont thrust belt in the southern margin of the Junggar Basin in Northwest China are taken as research objects. More than 60 MLJ samples were collected from outcrops and wells. Total organic carbon (TOC), rock pyrolysis (Rock–Eval), organic petrological, vitrinite reflectance (%Ro), and hydrous pyrolysis were performed to analyze the relevant samples. The pyrolysis gases and liquid products were measured, and then the chemical composition, as well as carbon isotopes of the gases, were analyzed. The results indicate that the MLJ source rocks have the capacity for large‐scale gas generation. In addition, for coal‐measure source rocks, the heavier the carbon isotope of kerogen (δ13Ckerogen), the lower the liquid hydrocarbon and hydrocarbon gas yield, and the easier it is to produce non‐hydrocarbon gas. It is worth noting that when the δ13Ckerogen in organic matter (OM) is relatively heavier, the fractionation of its products may become weaker in the evolutionary process. The vital contribution of the MLJ source rock to natural gas resources in the study area was further confirmed by comparing it with the Jurassic source gas.