This study aims to explore how the topic of sustainability in Higher Education Institutions (HEI) has been addressed in the scientific literature. It presents a systematic literature review of peer-reviewed journal articles published between 1975 and 2019. After that, bibliometric and content analyses were performed in order to identify the behavior and evolution of the literature and identify the frequency with which they appear in the text, making it possible to raise replicable and valid inferences to the data. The results of this study indicate that the theme is emerging since the year 2010 and with a strong focus on qualitative studies, focused on critical theory. The results indicate that although studies involve the tripod environment, economy, and society, there is still a gap in studies that deal with specific issues to solve practical problems and that analyze the impacts from a more holistic perspective. The combination of methods made it possible to identify and interpret the articles in the selected sample, but based on results reported by other authors, with little room for quality control and integrity.