ABSTRACT This study aims to determine the validity of the lower extremity joint kinematics measured by inertial measurement units (IMUs) in non-rearfoot strike pattern (NRFS) runners across different speeds. Fifteen NRFS runners completed three 2-min running tests on a treadmill in random order at 8, 10 and 12 km/h, whilst data were synchronously collected using the IMU system and an optical motion capture system. Before the offset was corrected, the validity of the knee angle waveform was higher than that of the hip and ankle; after the offset was corrected, the validity increased in all three joints. The correlation between the touchdown angles in the sagittal plane measured by the two systems was relatively high after the offset was corrected. The running speed influenced the offset-corrected measurements, with higher error values at higher speeds. The IMU system was able to provide measurements of running kinematics in the sagittal plane of NRFS runners at different running speeds but was unable to reliably measure motion in the frontal and horizontal planes. Future research should analyse the 3D gait of NRFS runners under a larger range of speed conditions to provide evidentiary support for the use of IMUs in running analysis outside the laboratory.