Joint Regulation Research Articles

Abstract 385: HIF1A can regulate Wnt signaling in human colon cancer cells

Abstract Standard-of-care treatment for KRAS mutant metastatic colorectal cancer (mCRC) combines a cytotoxic chemotherapy backbone (FOLFOX) with an antiangiogenesis biologic agent (i.e., bevacizumab). Despite significant efforts to improve treatment, the prognosis for this type of cancer remains poor with an average 5-year overall survival of ~11%. Oncogenic KRAS mutations lead to the overactivation of RAS/ERK/MAPK pathwa,y driving many hallmarks of cancer such as cell proliferation, growth, and altered metabolism. In particular, several studies have shown that oncogenic activation of KRAS leads to normoxic stabilization of HIF1A protein, which can then reprogram cellular metabolism towards aerobic glycolysis. Our studies have discovered that overactive Wnt signaling can also reprogram cancer cell metabolism towards aerobic glycolysis. Of note, KRAS (~40%) and Wnt signaling (~90%) are very frequently coactivated in colorectal cancer. Interestingly, an important, key metabolic gene targeted by Wnt signaling, Pyruvate Dehydrogenase Kinase I (PDK1), is also directly regulated by HIF1A, suggesting there may be crosstalk between the HIF1A and Wnt pathways. Indeed, several published studies support the idea that HIF1A can influence Wnt signaling, and our data show this may occur through regulation of the Wnt mediating transcription factors LEF1 and TCF1 (TCF7 gene). Using luciferase reporters driven by LEF/TCF promoters, we observed significantly increased LEF1/TCF7 promoter activity in hypoxic colon cancer cells but not for other TCF family members (TCF7L1/TCF7L2). We identified putative hypoxia responsive elements (HREs) in the human LEF1 promoter, and deletion of these HREs reduced responsiveness to hypoxia. Furthermore, we have found that targeting glycolysis and Wnt can be synergistic in inhibiting colon cancer cell colony growth. We find that HIF1A and LEF/TCF/beta-catenin complexes coregulate gene expression of metabolic targets as well as Wnt signaling components (e.g., LEF1 and TCF7). These data suggest that cross-regulation between the HIF1A and Wnt signaling pathway is important and may be a potential Achilles heel for novel therapeutics in targeting KRAS mutant colon cancer. Current studies are under way to further investigate the mechanisms of crosstalk and joint regulation of metabolic reprogramming in KRAS mutant colon cancer cells. Citation Format: Yung Lyou, George Chen, Marian Waterman. HIF1A can regulate Wnt signaling in human colon cancer cells [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 385.

Monitoring in collaborative learning: Co-occurrence of observed behavior and physiological synchrony explored

Although research on collaborative learning suggests that monitoring plays an important role in successful regulation of the collaborative learning process, little is known about how students attend to it together. This study explores monitoring in collaborative learning. Specifically, it studies how students in a group monitor their cognitive, affective and behavioral processes during their collaboration, as well as how observed monitoring co-occurs with their physiological synchrony during the collaborative learning session. Data was collected from 48 Finnish highschool students who were learning about nutrition in groups of three. The session was videotaped and coded in terms of monitoring of cognition, behavior and affect. Students’ arousal was measured as electrodermal activity with wearable sensors and used to calculate physiological synchrony between the students. Three case groups, with priority on the quality of the data, were chosen for detailed analysis. The results indicate that the main targets of monitoring for these case groups were cognition and behavior, while monitoring of affect occurred the least. Most of the student pairs inside the groups showed significant amounts of physiological synchrony. High values of physiological synchrony occurred when monitoring was frequent. Time series analysis showed a weak positive connection between monitoring and physiological synchrony for two groups out of three. These results indicate that physiological synchrony could potentially shine a light on the joint regulation processes of collaborative learning groups.

How Can the Organizing Work Involved in the Joint Regulation of Lean Projects Promote an Enabling Organization and Occupational Health?

The objective of this article is, through an empirical study, to further understanding of the actions and decisions taken in the context of Lean implementation projects carried out under joint regulation (Lévesque and Murray, 1998) agreements. We, therefore, attempt to identify factors that may facilitate the organizing work involved in joint regulation of Lean projects to allow workers to develop a broader range of health-minded work methods and habits. Our assumption is that factors which influence joint regulation, such as the union’s capacity for action, management’s attitude and the purpose of the change, also influence the occupational health outcomes of Lean projects. We believe that the organizing work involved in joint regulation (actions and decisions) has an impact on these factors and influences the occupational health outcomes. Our research question is therefore this: What are the actions and decisions involved in joint regulation of Lean implementation projects that lead to closer correspondence with enabling organization criteria? This empirical study was exploratory in nature and had a multiple case study design. Two cases of lean projects were documented through eight individual interviews and the collection of documents. The main results indicate that, while joint regulation appears essential in terms of meeting enabling organization criteria, it alone is insufficient to explain the health effects of Lean projects. All stakeholders need to define the project goals, modes of assessment and management rules, both cooperatively and transparently, and through their involvement in decisions regarding all processes.

Regulating full contact martial arts and combat sports: how governmentality as an analytical framework informs practices of the governing of sports

ABSTRACTThe governing of sport has recently shifted from self-regulation towards various forms of joint regulation. This study uses the concept of governmentality to analyse practices of governing full contact martial arts and combat sports (FCMACS) in the Netherlands to answer the question of how the concept of governmentality informs the current and future governing of FCMACS. Dean’s methodological guidelines were used in the analysis of the consensus and contradictions in the rationalities and related technologies of power (regimes of practices) expressed in the regulation of FCMACS. According to the study’s respondents, future practices of the governing of FCMACS must enact equitable restrictions for all gyms and fight events and highlight the social value of FCMACS to facilitate them gaining the same status as regular sports. Such change requires practices of governing that combine authority and mediation with a climate open to self-regulation.

CDK and MAPK Synergistically Regulate Signaling Dynamics via a Shared Multi-site Phosphorylation Region on the Scaffold Protein Ste5

We report an unanticipated system of joint regulation by cyclin-dependent kinase (CDK) and mitogen-activatedprotein kinase (MAPK), involving collaborative multi-site phosphorylation of a single substrate. In budding yeast, the protein Ste5 controls signaling through a G1 arrest pathway. Upon cell-cycle entry, CDK inhibits Ste5 via multiple phosphorylation sites, disrupting its membrane association. Using quantitative time-lapse microscopy, we examined Ste5 membrane recruitment dynamics at different cell-cycle stages. Surprisingly, in S phase, where Ste5 recruitment should be blocked, we observed an initial recruitment followed by a steep drop-off. This delayed inhibition revealed a requirement for both CDK activity and negative feedback from the pathway MAPK Fus3. Mutagenesis, mass spectrometry, and electrophoretic analyses suggest that theCDK and MAPK modify shared sites, which aremost extensively phosphorylated when both kinasesare active and able to bind their docking siteson Ste5. Such collaborative phosphorylation can broaden regulatory inputs and diversify output dynamics of signaling pathways.

The joint regulation of bank liquidity and bank capital

Abstract We study the liquidity behavior of commercial banks in response to negative capital shocks. Using pre-Basel III data, U.S. banks with assets less than $1 billion treated (unregulated) liquidity and (regulated) capital as substitutes. Following exogenous shocks to their regulatory capital ratios, these banks shifted away from loans, loan commitments, and dividend payouts, actions that both repaired their capital ratios and enhanced their liquidity positions. We find little similar behavior at larger banks. We conclude that a minimum capital constraint naturally mitigates liquidity risk at community banks, justifying the exemption of these banks from the Basel III liquidity standards.

Organic fertilizer application increases the soil respiration and net ecosystem carbon dioxide absorption of paddy fields under water-saving irrigation.

Quantifying carbon sequestration in paddy soil is necessary to understand the effect of agricultural practices on carbon cycles. The objective of this study was to assess the effect of organic fertilizer addition (MF) on the soil respiration and net ecosystem carbon dioxide (CO2) absorption of paddy fields under water-saving irrigation (CI) in the Taihu Lake Region of China during the 2014 and 2015 rice-growing seasons. Compared with the traditional fertilizer and water management (FC), the joint regulation of CI and MF (CM) significantly increased the rice yields and irrigation water use efficiencies of paddy fields by 4.02~5.08 and 83.54~109.97% (p < 0.05). The effects of organic fertilizer addition on soil respiration and net ecosystem CO2 absorption rates showed inter-annual differences. CM paddy fields showed a higher soil respiration and net CO2 absorption rates during some periods of the rice growth stage in the first year and during most periods of the rice growth stage in the second year. These fields also had significantly higher total CO2 emission through soil respiration (total Rsoil) and total net CO2 absorption compared with FC paddy fields (p < 0.05). The total Rsoil and net ecosystem CO2 absorption of CM paddy fields were 67.39~91.55 and 129.41~113.75molm-2, which were 27.66~135.52 and 12.96~31.66% higher than those of FC paddy fields. The interaction between water and fertilizer management had significant effects on total net ecosystem CO2 absorption. The frequent alternate wet-dry cycles of CI paddy fields increased the soil respiration and reduced the net CO2 absorption. Organic fertilizer promoted the soil respiration of paddy soil but also increased its net CO2 absorption and organic carbon content. Therefore, the joint regulation of water-saving irrigation and organic fertilizer is an effective measure for maintaining yield, increasing irrigation water use efficiency, mitigating CO2 emission, and promoting paddy soil fertility.

Joint Regulation of Power Flow and Short Circuit Current Limitations with the Aid of a Phase-Shifting Transformer

The efficiency of installation of the phase-shifting transformer (PST) in the power supply system of St. Petersburg and the Leningrad Oblast’ is examined, making it possible to execute power flow management within the specified range and limit short-circuit currents to values that do not exceed the breaking capacity of the switching equipment. Its optimal parameters were found, based on analysis of various PST operating modes. Proceeding from this, design calculation of the magnetic core and transformer windings was performed.

Cooperative Decision Making of DERs in a Joint Energy and Regulation Market in Presence of Electric Vehicles

Cooperative Decision Making of DERs in a Joint Energy and Regulation Market in Presence of Electric Vehicles

Research on the Comprehensive Effect of Ecological Regulation in the Mainstream of Weihe Basin

The Weihe River is the largest tributary of Yellow River in China. Due to the decrease in water resources and social-economic development, water resources situation in the Weihe River Basin is more serious now than ever before. Water use for the hydro-ecological system has been obviously decreased because of the reduction in water resources and the increase in social-economic water consumption. Reservoir ecological regulation is an effective measure to preserve river health and to realize man-water harmonization. Based on ecological regulation theory, principles of ecological regulation were presented according to the different levels of social-economic development in the Weihe River Basin. The regulation approaches for ecological water use were analyzed in terms of water saving, recycled water reuse, joint regulation of reservoirs, conjunctive operation of surface water and groundwater, controlling intake of hydropower and inter-basin water transfer project. Water regulation model of Weihe River Basin was constructed. Water regulation scenarios were set. With the ecological basic flow (EBF) targets of the key sections on the mainstream, the balance of water supply-demand, water supply for important reservoirs and the monthly guarantee rate of ecological flow were analyzed. Although further study is desired, the results are believed to be of great referential value to sustainable development in the basin.

Peraturan Yang Ditetapkan oleh Kepala Desa (Kajian Terhadap Pasal 8 ayat (1) UU No 12 Tahun 2011 Tentang Pembentukan Peraturan Perundang-Undangan)

Article 2 Domestic Ministerial Regulation Number 111 of 2014 concerning technical guidelines for Village Regulations states that Type of Regulation in the village includes: a. Village Regulation b. Joint Regulation of the Village Head; and c. Village Head Regulation. Meanwhile, Article 8 paragraph (1) of the Law Establishing Legislation states that the type of legislation also includes the rules set by ... Village Heads or the equivalent. Based on the construction of norms Article 8 paragraph (1) of the Law on Establishment of Legislation, village regulations cannot be categorized as regulations established by the village head because a village regulation must have a joint agreement between the village head and the village consultative body and must be promulgated in Village and legal sheets become village regulations even though they are not stipulated by the village head.

A bi-level stochastic framework for VPP decision making in a joint market considering a novel demand response scheme

Summary This paper presents a stochastic decision-making framework for an optimal bidding strategy of a virtual power plant (VPP) in a joint day-ahead and regulation (balancing) market. The VPP seeks to maximize its expected profit in day-ahead market and compensates its deviation in balancing market. Due to the inherent uncertainties of renewable energy resources and also other market participant strategies, a stochastic programming is used for underlying optimization problem. In this regard, a 2-stage bi-level problem is presented in which upper level represents VPP profit maximization and lower level deals with market clearing problem in which power transfer distribution factors are used to incorporate transmission constraints. Uncertainties related to stochastic generation and market participant offer/bid curves are modeled via scenarios. A mathematical programming with equilibrium constraints is obtained by reformulating the lower-level problem using Karush-Kuhn-Tucker optimality conditions. The resulting mathematical programming with equilibrium constraints is converted into a tractable mixed-integer linear programming problem with strong duality theorem. The considered VPP is assumed to be commercial and consists of stochastic generation units (wind and solar), conventional power plant, energy system storage, and adjustable internal loads. A novel demand response scheme is introduced into the VPP portfolio in which VPP is penalized through shifting load amount and operating time interval as well. Finally, a trade-off between profit and risks associated with uncertainties is explicitly taken into account using the conditional value at risk. To assess the validity and the effectiveness of the proposed model, a 6-bus test system is chosen to apply the model.

PERATURAN YANG DITETAPKAN OLEH KEPALA DESA (Kajian Terhadap Pasal 8 ayat (1) UU No 12 Tahun 2011 Tentang Pembentukan Peraturan Perundang-Undangan)

Abstract : Article 2 Domestic Ministerial Regulation Number 111 of 2014 concerning technical guidelines for Village Regulations states that Type of Regulation in the village includes: a. Village Regulation b. Joint Regulation of the Village Head; and c. Village Head Regulation. Meanwhile, Article 8 paragraph (1) of the Law Establishing Legislation states that the type of legislation also includes the rules set by ... Village Heads or the equivalent. Based on the construction of norms Article 8 paragraph (1) of the Law on Establishment of Legislation, village regulations cannot be categorized as regulations established by the village head because a village regulation must have a joint agreement between the village head and the village consultative body and must be promulgated in Village and legal sheets become village regulations even though they are not stipulated by the village head.

Pendapat Pimpinan-pimpinan Gereja di Bekasi tentang Izin Pendirian Rumah Ibadah dalam Peraturan Bersama Menteri Tahun 2006

ABSTRACT:The article, entitled "Opinion The church Leaders in Bekasi on the Permit Construction of Houses of Worship in the Joint Regulation of the Minister of the Year 2006, will be explained about the implementation of policies permit the establishment of houses of worship in Joint Regulation of the Minister (PBM) in 2006 in Bekasi, as well as the implications for church in that place, through the opinion of the church leaders in Bekasi. First, authors will describe the Guarantee Rights of Religion, Belief, Worship and Establish Home Worship based on Pancasila and the Indonesian Constitution, the Universal Declaration of Human Rights, The Covenant, and documents declarations of the United Nations (UN), and also explained about permit the establishment of houses of worship of Letters Joint Decree (SKB) until the PBM. After that, will be presented regarding the results of the opinion of the church leaders in Bekasi on the implementation of the policy permits a house of worship in Bekasi. Opinion leaders in Bekasi Church will be grouped in four categories, namely: A) The church which has a license, and not problematic. B) The church is having problems obtaining a license, a problem with the public, but finish. C) The church that does not have a license but has no problem with the community. D) The church that does not have permissions and have problem with the community, and did not finish. The findings obtained are church leaders in the four categories above found PBM on authorizing the establishment of houses of worship contrary to Pancasila and the Indonesian Constitution and the values of human rights are universal, and implementation of government policies that adversely affect the lives of religious believers in Bekasi, both in the internal relations of religion, as well as in interreligious relations. KEYWORDS: permit the establishment of houses of worship, Joint Ministerial Decree, Pancasila, the Indonesian constitution, human rights

Evaluasi terhadap Peraturan Bersama Menteri Tahun 2006 tentang Pendirian Rumah Ibadah

ABSTRACT:Â This article with a title Evaluation to the Joint Regulation of Ministers in 2006 about the Establishment of a House of Worship will focus on the evaluation of the formulation of the Joint Regulation of Ministers in 2006 about the Establishment of a House of Worship (Peraturan Bersama Menteri PBM), as well as the evaluation of the application and impact of the enacted regulation. The research methodology uses qualitative research with an in-depth interview to some church leaders in Bekasi, West Java, who are directly connected with the enactment of this regulation, and also research to the related documents with the PBM, especially the policy wich founded the PBM. The research finding is the formulation of the Joint Regulation of Ministers about the Establishment of a House of Worship has not yet fitted with the formulation policy model of a democratic country so that its application is not in accordance with the expected goal. PBM application gives negative impact to the inter-religious harmony in Indonesia. KEYWORDS: regulation of the esthablisment of a house of worship, freedom of worship, policy evaluation

Start-up and Capacity Enhancement of a Partial Nitrification Pilot Reactor in Continuous Flow

The continuous flow reactor was used to treat simulated ammonia nitrogen wastewater by inoculating the sludge after filtration and adding a suspended filler. Regulations of free ammonia (FA), free nitrous acid (FNA), and dissolved oxygen (DO) in the reactor were the key to achieving a successful start-up of the pilot scale nitrosation reactor. The results show that the enrichment of ammonium oxidizing bacteria (AOB) and the elimination of nitrite oxidizing bacteria (NOB) are achieved by adjusting the operational mode of high DO, low DO, FA, and FNA in the reactor operation. The nitrite production rate (NPR) in the reactor was 1.27 kg·(m3·d)-1 and the nitrogen accumulation rate (NAR) was stable at 98% at the end of the start-up period. qPCR was used to study the difference in the functional microorganisms (AOB, NOB) between the beginning and the end of the start-up period. The results show that the copy number of microbial AOB grew from 5.3×109 copies·mL-1 to 1.6×1011 copies·mL-1. The copy number of NOB decreased from 1.1×1010 copies·mL-1 to 1.2×109 copies·mL-1, because of the joint regulation of DO, FA, FNA to suppress NOB.

Enhancing frequency stability by integrating non-conventional power sources through multi-terminal HVDC grid

The 2050 targets established by the EU will foster both larger penetration of renewable energy, especially wind power, and more cross-border interconnections. Moreover, this new framework requires the non-conventional power sources and power converter-based systems to be responsible for the duties traditionally carried out by conventional synchronous generators as frequency support. This paper presents how different power-electronic based technologies can provide frequency support individually and in a coordinated manner (with different priority given by the deadbands) ensuring a stable operation.The implemented scenarios examine challenging conditions, where the primary reserve of the interconnected conventional, renewable, and storage generation is fully utilized to tackle frequency incidents. This demonstrates how the joint regulation of the power electronic-based technologies enhances the frequency stability of the AC synchronous areas. The different control schemes and their interaction are investigated in Cigré DC grid benchmark adapted for frequency stability studies and implemented in Matlab/Simulink simulation tool. This modified grid includes 5-terminal HVDC grid with two offshore wind farms and three AC networks including battery and onshore wind farms.

Cooperation of Wind Power and Battery Storage to Provide Frequency Regulation in Power Markets

In the future power system with high penetration of renewables, renewable energy is expected to undertake part of the responsibility for frequency regulation, just as the conventional generators. Wind power and battery storage are complementary in accuracy and durability when providing frequency regulation. Therefore, it would be profitable to combine wind power and battery storage as a physically connected entity or a virtual power plant to provide both energy and frequency regulation in the markets. This paper proposes a real-time cooperation scheme to exploit their complementary characteristics and an optimal bidding strategy for them in joint energy and regulation markets, considering battery cycle life. The proposed cooperation scheme is adopted in a real-time battery operating simulation and then incorporated into the optimal bidding model. The scheme could improve the wind regulation performance score and allow for more regulation bids without affecting the battery life, thus significantly increasing the overall revenue. The validity of the proposed scheme and strategy are proved by the case study.

Intelligent safety adjustment of branch airflow volume during ventilation-on-demand changes in coal mines

Abstract In view of the frequent ventilation-on-demand (VOD) changes of branches during intensive production in coal mines, the models for regulating the ventilation resistance of associated branches and ventilator frequency were established. Automated ventilation system adjustment software has been developed by monitoring the air volume of the minimum remainder branch in the network, and all air volumes of the branches in the network can be obtained by inverse calculation of associated branches. When the VOD of branches changes, the best regulating branch in the ventilation network is selected by a cellular automation (CA) model, and the ventilation resistance is determined by ventilation network calculation. It is also possible to calculate the total air volume of the ventilation system, according the VOD of the branch to calculate the ventilator frequency. Advanced simulation regulating results are verified by ventilation network calculation. The ventilation resistance of associated branches, ventilator frequency, and joint regulation are simulated by the automated ventilation system adjustment software. A pipe network ventilation parameter monitoring system is designed and developed based on typical ventilation network models. We confirm the VOD of the branch based on the absolute gas emission volume prediction model and then establish a regulating model for the ventilation resistance of associated branches and the ventilator frequency. Considering the fire and explosion risks associated with gas, CO2 is instead released to simulate gas emission in the experiment; gas concentration is controlled by the ventilation resistance of associated branches and ventilator frequency. These results verify that CO2 concentration is kept below the threshold value by increasing the air volume of the branch, the feasibility of the ventilation system, and regulating the VOD of the branch; additionally, they provide theoretical guidance for adjusting ventilation branch quantity by the association branches of ventilation resistance and ventilator frequency.

Role and Significance of Ikaros Family in the Development of Hematopoietic System -Review

Hematopoietic cells are regulated by many transcriptional factors during their development, among them the Ikaros family is one of the most important representatives. They have characteristic conserved structural motifs, binding with DNA specific short sequences-containing key gene promoter or enhancer, to regulate their transcription activity. Meanwhile, the Ikaros family interact with other related transcriptional regulators to regulate the development and differentiation of hematopoietic cells. The structure of the Ikaros family, which belong to the zinc finger transcription factor, including Ikaros, Helios, Aiolos, Eos and Pegasus, are encoded by IKZF1-5 genes, respectively. They are master regulators of hematopoiesis, playing important roles in the occurrence, development and function of hematopoietic cells such as lymphocytes via individual and joint regulation. When working abnormalities, they are often related with the occurrence and development of the disease. In this review, the research achievements of the Ikaros family in recent years are summarized. On the one hand, it is helpful to understand the role and significance of this family in hematopoietic system; on the other hand, it provides the possible research direction for further research work.