This article is devoted to the analysis of the legal regulation of law enforcement cooperation within the EU, where there is an integral system of organizational, legal, technical and informational mechanisms that allow the member states and the European Union in general to achieve effective results in the protection of security on the territories of the EU as a whole and state- members in particular. Three main areas, namely the European Multidisciplinary Platform Against Criminal Threats (EMPACT), the problem of unification and harmonization of legal regulation of cross-border cooperation, for example within the framework of joint investigative operations, the activities of special supranational institutions in this field in the article is being investigated. In particular, the content of the fundamental regulatory act in this area was analyzed - the Code of Police Cooperation, which establishes the legal regulation of cross-border harassment and surveillance, the procedure for conducting joint investigative operations and establishing joint training and professional development. Considerable attention is dedicated to the problem of the common information space of law enforcement cooperation, within which the SIS information system was created, the SIRENE bureau operates, and a number of other supranational tools. The European practice of combating modern challenges of criminal and legal threats is studied in the article as an example of high standards of law enforcement cooperation in the fight against cross-border crime and guaranteeing the safety of human life for Ukraine. In order to introduce such standards, a holistic study of the entire legal array regulating the cooperation of law enforcement agencies within the EU is necessary, with the aim of harmonizing the law of Ukraine in accordance with the Association Agreement, in particular, Chapter III "Justice, Freedom and Security". On the other hand, Ukraine should become a pillar of European security, given the incredible threat to Europe from the aggressor country Russia. Europe seriously thought about its security and the formation of a joint army and rapid response forces. That is why the security of Europe today depends on the joint close cooperation of the EU and Ukraine.