Jasonia Glutinosa Research Articles

Jasonia glutinosa (L.) DC., a Traditional Herbal Tea, Exerts Antioxidant and Neuroprotective Properties in Different InVitro and In Vivo Systems.

Simple SummaryJasonia glutinosa (L.) DC or rock tea (RT) is a plant traditionally used to treat different pathologies. In this study the neuroprotective potential of an ethanolic extract of RT is analyzed. Caenorhabditis elegans model and in vitro assays with relevant central nervous system enzymes were used. The results showed antioxidant and neuroprotective potential of this plant.In traditional medicine, Jasonia glutinosa (L.) DC or rock tea (RT) has been mainly used to treat digestive and respiratory pathologies but also as an antimicrobial or an antidepressant herbal remedy. An ethanolic extract of RT has been demonstrated to have antioxidant and anti-inflammatory effects, which may be explained by its phytochemical profile, rich in polyphenols and pigments. The aim of this study is to investigate the neuroprotective potential of RT. For this purpose, the ethanolic extract of RT is assayed in Caenorhabditis elegans (C. elegans) as an in vivo model, and through in vitro assays using monoamine oxidase A, tyrosinase and acetylcholinesterase as enzymes. The RT extract reduces juglone-induced oxidative stress in worms and increases the lifespan and prevents paralysis of C. elegans CL4176, a model of Alzheimer’s disease; the extract is also able to inhibit enzymes such as acetylcholinesterase, monoamine oxidase A and tyrosinase in vitro. Together these results demonstrate that Jasonia glutinosa is a good candidate with antioxidant and neuroprotective potential for the development of new products with pharmaceutical interests.

Effect of Jasonia glutinosa on immune and oxidative status of gilthead seabream (Sparus aurata L.)

Jasonia glutinosa (rock-tea, RT) has numerous biological activities. In the present work, the beneficial effects of dietary RT on gilthead seabream (Sparus aurata L.) were studied. Fish fed experimental diets containing 0 (control), 10 or 30% of RT for 15 and 30 days. Samples from skin mucus, blood, head kidney, liver and gut were obtained at 15 and 30 days. The antioxidant properties from RT were analysed such as the antioxidant capacity and phenolic content. The heat shock protein 70 level (HSP70) and the total oxidized proteins were evaluated on skin mucus as stress markers. Immune parameters, both humoral (peroxidase activity, immunoglobulin M levels and complement activity) and cellular (phagocytic, respiratory burst and peroxidase activities) were determined in skin mucus, serum or head-kidney leucocytes, respectively. Concomitantly, the expression of different genes related to inflammation and oxidative stress was studied both in liver and gut. Skin mucus peroxidase was significantly increased on fish fed 10% RT for 15 days with respect to the control group. In addition, Serum IgM levels were significantly increased while HSP70 levels and oxidized proteins were significantly decreased on skin mucus from fish fed 30% RT for 30 days, respectively. Besides, cellular immune parameters (phagocytosis, respiratory burst and peroxidase activity) were significantly higher in leucocytes from fish fed the RT diets for 15 days, but not for 30 days. Finally, the gene expression of antioxidant enzymes was up-regulated in liver at 15 and in liver and gut at 30 days. However, the expression of il1b and hsp70 was down-regulated in the liver of fish fed 30% RT for 30 days with respect to the values of control fish. The possible inclusion of RT in fish diets as an additive with antioxidant and/or immunostimulant activities is discussed.

Jasonia glutinosa (L.) DC., a traditional herbal medicine, reduces inflammation, oxidative stress and protects the intestinal barrier in a murine model of colitis.

Jasonia glutinosa (L.) DC., known as rock tea (RT), is traditionally used in Spain as a digestive due to its beneficial properties in bowel disorders. The pharmacological nature of these properties has not been established yet. The aim of this work was to evaluate the therapeutic utility of RT in experimental colitis and to identify chemical constituents with anti-inflammatory and/or anti-oxidative properties. RT extract was prepared with ethanol in a Soxhlet apparatus and analysed by HPLC-DAD. Superoxide radical scavenging properties, xanthine oxidase and lipoxygenase (5-LOX) inhibitory activity, and capability to lower nitric oxide (NO) and tumor necrosis factor α (TNF-α) levels were measured in cell-free and cell-based assays. In the 2.5%-dextran-sodium sulphate (DSS) injury-repair model of ulcerative colitis (UC), mice were daily treated with sulfasalazine (SSZ, as reference drug, 100mg/kg bw), RT (5, 25 and 50mg/kg bw, p.o.), or vehicle over 20 days. Colitis was scored daily. Colon samples were examined macroscopically and histopathologically. Protein levels of myeloperoxidase (MPO), interleukins 6, and 10 (IL-6, IL-10), inducible NO synthase (iNOS), and cyclooxygenase-2 (COX-2) were studied as markers of oxidative stress and inflammatory activity. The integrity of the apical epithelial layer was assessed by immunofluorescence staining of zonula ocludens-1 (ZO-1). Finally, intestinal contractility was also evaluated by isometric myography. Fifteen phenolic compounds and three pigments were identified and quantified, of which caffeoylquinic acids, and the flavonoid, quercetin-3-O-galactoside, were the most abundant. RT extract significantly scavenged superoxide radicals, inhibited 5-LOX activity, and lowered NO and TNF-α levels. DSS-treated mice receiving RT scored clinically lower than controls during the first 3 days of DSS treatment and during the recovery period. SSZ was less effective than RT. Anatomical and histological examination of colon samples revealed that RT significantly prevented colon shortening, increased colon thickness, and lowered the macroscopic damage score. RT also significantly prevented the increase of MPO activity, IL-6 levels, iNOS and COX-2 expression, the loss of ZO-1 apical expression, and normalized contractility disturbances. In conclusion, daily administration of RT showed therapeutic properties in the DSS-model of UC. The benefits of RT can likely be attributed to its anti-inflammatory and antioxidant phenolic and flavonoid constituents.

Medicinal and local food plants in the south of Alava (Basque Country, Spain)

Ethnobotanical relevanceMedicinal and food plants in the Basque Country are an integral part of a fast changing culture. With a distinct tradition and language, this region of Europe provides an important example demonstrating the changing role of local and traditional knowledge in industrial countries. As other Mediterranean regions it preserves a rich heritage of using plants as medicine and food, offering a unique opportunity for studying the medicine food interface in an ethnopharmacological context. Therefore, the key goal of this study has been to contribute to an understanding of local and traditional plant usage, to evaluate their uses as food and medicine as well as to critically assess the role of these plants in the south of the Basque Country contributing to an understanding of how foods and medicines are used. MethodsA mixed methods approach, including participant observation; open and semi structured interviews was used. Ethnobotanical field work included 183 people, ages ranged from 24 to 98 years old with a majority being between 70 and 80 years old (mean age 71) from 31 towns of three different regions. The basic interview was a one-to-one meeting, which often included field walking and collection of samples as directed by the informants. 700 voucher specimens (most of them with duplicates) were collected for the data obtained.Using SPSS version 20 the gathered information was processed and the replies of the different informants were subsequently organised in variables like medicine and food plants, part of the plants used, forms of preparations, zones preferred for collecting these plants. The data were analysed based on the frequency of records. This type of approach allows us to understand the way the informant’s categorize the species, and how these categories are distributed along the sample. In order to analyse the data three main categories of use were distinguished: Medicine (M), Food (F) and an intermediate Health-Food (H-F). The three categories were divided in 27 subcategories (common uses). Results and discussionThe informants recognise and use a total of 184 species from 49 families. During interviews, 5658 individual use-reports were collected relating to three use-categories – as medicines, food and health-food. The two main groups with almost the same number of species each are health-food (75 species) and (locally gathered) food only (73), with medicinal uses only (36) being the smallest group. This highlights the important overlap between food and medicines.Overall, three core families were identified (based on the number of use reports and in the number of species): Asteraceae (25 species), Lamiaceae and Rosaceae (24 each). The most frequently reported species are Jasonia glutinosa, Chamaemelum nobile, Prunus spinosa and Quercus ilex subsp. ballota.The most important general use-subcategories are as raw vegetables (27.43% of the use-reports and including 81 species), infusions (14.74%/42) and gastrointestinal (12.53%/42). Conceptually foods and medicines are clearly distinguished but the intermediate group of health foods is more ambiguous. ConclusionFood and medicinal uses of plants are culturally closely linked. A wide range of plants are known and many still used. The analysis shows that the Basques use a wide range of species which are typical for Western European cultures. In comparison to other studies in the Mediterranean countries there are many similarities in the uses of different families, species of plants and their use and preparations. Some of these plants are key Mediterranean species, often used for a multitude of uses as food and medicine.

Rock Tea extract (Jasonia glutinosa) relaxes rat aortic smooth muscle by inhibition of L-type Ca(2+) channels.

In traditional herbal medicine, Rock Tea (Jasonia glutinosa) is known for its prophylactic and therapeutic value in various disorders including arterial hypertension. However, the mechanism by which Rock Tea exerts blood pressure-lowering actions has not been elucidated yet. Our aim was to demonstrate vasorelaxing effects of Rock Tea extract and to reveal its possible action mechanism. Isometric myography was conducted on high-K+-precontracted rings from rat thoracic aorta and tested extracts at concentrations of 0.5-5 mg/ml. Whole-cell patch-clamp experiments were performed in rat aortic vascular smooth muscle cells (line A7r5) to determine blocking effects on L-type Ca(2+) channels. Rock Tea extract relaxed the aorta contracted by high [K+] concentration dependently with an EC50 of ≈2.4 mg/ml and produced ≈75 % relaxation at the highest concentration tested. The L-type Ca(2+) channel blocker, verapamil (10(-6) M), had similar effects. Rock Tea extract had no effect in nominally Ca(2+)-free high-K(+) buffer but significantly inhibited contractions to re-addition of Ca(2+). Rock Tea extract inhibited the contractions induced by the L-type Ca(2+) channel activator Bay K 8644 (10(-5) M) and by phenylephrine (10(-6) M). Rock Tea extract and Y-27632 (10(-6) M), Rho-kinase inhibitor, had similar effects and the respective effects were not additive. Patch-clamp experiments demonstrated that Rock Tea extract (2.5 mg/ml) virtually abolished L-type Ca(2+) currents in A7r5. We conclude that Rock Tea extract produced vasorelaxation of rat aorta and that this relaxant effect is mediated by inhibition of L-type Ca(2+) channels. Rock Tea extracts may be of phytomedicinal value for prevention and adjuvant treatment of hypertension and other cardiovascular diseases.

Medicinal plants used for neurological and mental disorders in Navarra and their validation from official sources

Ethnopharmacological relevanceThis paper provides important ethnopharmacological information on plants used in neurological and mental disorders in Navarra. Material and methodsInformation was collected using semi-structured ethnobotanical interviews with 667 informants in 265 locations. In order to confirm the pharmacological validation of the uses claimed by the informants, monographs from Official International Agencies (ESCOP, Commission E, WHO and EMA) were reviewed. A literature review was conducted focusing on the plants that were widely used but had no published monograph. ResultsA total of 172 pharmaceutical uses were reported, for 46 plants and 26 families, mainly represented by Lamiaceae (15%), Asteraceae (13%), Rosaceae and Rutaceae (7%, each one), and Clusiaceae, Malvaceae, Papaveraceae and Urticaceae (4%, each one). The most frequently used parts were inflorescence (39%), flowered aerial parts (16%), and aerial parts (13%), followed by inflorescence bract (8%) and leaves (7%). Nine out of 46 plants (20%) and 81 of 172 uses (47%), have already been pharmacologically validated. The remaining 37 plants (of total 46, 80%) have been reported for neurological and mental disorders and need to be screened through standard pharmacological and clinical procedures for their activities. The most used species are Chamaemelum nobile (L.) All., Jasonia glutinosa (L.) DC., and Santolina chamaecyparissus L. ssp. squarrosa (DC.) Nyman, in all cases the administration as infusion. ConclusionsData indicate a high degree of plants knowledge in Navarra regarding neurological and mental disorders. The present study constitutes a good basis for further phytochemical and pharmacological research of C. nobile, J. glutinosa and S. chamaecyparissus, which could be of interest in the design of new inexpensive, effective and safe drugs.

The pharmacological validation of medicinal plants used for digestive problems in Navarra, Spain

IntroductionTo provide significant ethnopharmacological information on the plants used to treat digestive problems in the Navarra region of Spain. Materials and methodsInformation was collected using semi-structured, ethnobotanical interviews with 667 informants (mean age 72; 55.47% women, 44.53% men) in 265 locations. Monographs from the European Scientific Cooperative on Phytotherapy (ESCOP), the German Commission E, the World Health Organization (WHO), the European Medicines Agency (EMA), the European Pharmacopoeia (Ph. Eur.) and the Real Farmacopea Española (RFE) were used to confirm and validate the pharmacological actions for the reported uses of these plants. In cases when frequently reported plants were not covered by a monograph, a literature review was performed using a new tool from the University of Navarra: the UNIKA database. ResultsA total of 1214 pharmaceutical uses were reported by the informants of this study; these uses originated from 126 plants and 47 families and were mainly represented by Asteraceae, Lamiaceae and Rosaceae. The most frequently used parts of the plants were the inflorescences, followed by the flowered aerial parts and fruits. Thirty-three out of 126 plants (26%) and 322 of their identified 1214 popular uses (27%), have already been pharmacologically validated. ConclusionsThe authors propose that four species should be explored and validated (Santolina chamaecyparissus ssp. squarrosa, Jasonia glutinosa, Jasonia tuberosa and Prunus spinosa) because these species are frequently mentioned and show promise for therapeutic treatments.

Pharmaceutical ethnobotany in the Middle Navarra (Iberian Peninsula)

Ethnopharmacological relevanceThis paper provides significant ethnobotanical information on pharmaceutical plant uses in the Middle Navarra (Iberian Peninsula). Collect, analyze and evaluate the ethnobotanical knowledge about medicinal plants in this area with 3622.2km2 and 404,634 inhabitants. Materials and methodsWe performed semi-structured interviews with 276 informants (mean age 72; 46% women, 54% men) in 111 locations, identified the plant reported and analyzed the results, comparing them with those from other territories. ResultsThe informants reported data on 198 medicinal plants belonging to 60 botanical families. This work is focused on human medicinal plant uses, which represent 98% of the pharmaceutical uses (1401 use reports). The species with the highest number of cites are Santolina chamaecyparissus ssp. squarrosa, Jasonia glutinosa and Chamaemelum nobile with a long tradition of use in Navarra. All different plant parts are used; aerial part is exploited more frequently than other plant parts. Most of the listed remedies use a single ingredient, typically soaked in water. The most common mode of administration is oral, while the second most common is topical. ConclusionsThe main ailments treated are digestive troubles, wounds and dermatological problems, and respiratory affections. Informants reported 80 new or scarcely cited uses for 14 medicinal plants. For 36% of the species (5) we have not found bibliographical references in the scientific literature and 64% (9) have only one to three references.

Screening of Spanish Medicinal Plants for Antioxidant and Antifungal Activities

Dichloromethane, ethyl acetate, methanol, and aqueous extracts obtained from 16 Spanish medicinal plants were screened for their antioxidant and antifungal activities. The radical scavenging capacity was evaluated by the DPPH method using a rapid screening by TLC and a spectrophotometric assay. Polar extracts obtained from Jasonia glutinosa L. (Lamiaceae), Tanacetum parthenium (L.) Schultz (Lamiaceae), Equisetum telmateia Ehrh. (Equisetaceae), Verbena officinalis L. (Verbenaceae), and Lythrum salicaria L. (Lythraceae) showed high antioxidant properties. Among them, the methanol extract of Lythrum salicaria showed the strongest antiradical capacity with an IC50 value similar to the positive control ascorbic acid. On the contrary, the best antifungal properties against Rhizopus stolonifer were produced by ethyl acetate or dichloromethane extracts from Anthemis arvensis L. subsp. arvensis (Asteraceae), Tanacetum parthenium, Santolina chamaecyparissus L. subsp. squarrosa Nyman (Asteraceae), Anagallis arvensis L. (Primulaceae) and the methanol extract of Anagallis foemina Miller (Primulaceae). The dichloromethane extract of Anthemis arvensis subsp. arvensis was the best inhibitor of fungus growth.

Analyzing factors that influence the folk use and phytonomy of 18 medicinal plants in Navarra

BackgroundThis article analyzes whether the distribution or area of use of 18 medicinal plants is influenced by ecological and cultural factors which might account for their traditional use and/or phytonymy in Navarra.This discussion may be helpful for comparative studies, touching as it does on other ethnopharmacological issues: a) which cultural and ecological factors affect the selection of medicinal plants; b) substitutions of medicinal plants in popular medicine; c) the relation between local nomenclature and uses. To analyze these questions, this paper presents an example of a species used for digestive disorders (tea and camomile: Jasonia glutinosa, J. tuberosa, Sideritis hyssopifolia, Bidens aurea, Chamaemelum nobile, Santolina chamaecyparissus...), high blood pressure (Rhamnus alaternus, Olea europaea...) or skin diseases (Hylotelephium maximum, H. telephium, Anagallis arvensis, A. foemina).MethodsFieldwork began on January 2004 and continued until December 2006. During that time we interviewed 505 informants in 218 locations in Navarra. Information was collected using semi-structured ethnobotanical interviews, and we subsequently made maps using Arc-View 8.0 program to determine the area of use of each taxon. Each map was then compared with the bioclimatic and linguistic map of Navarra, using the soil and ethnographic data for the region, and with other ethnobotanical and ethnopharmacological studies carried out in Europe.ResultsThe results clearly show that ecological and cultural factors influence the selection of medicinal plants in this region. Climate and substrate are the most important ecological factors that influence the distribution and abundance of plants, which are the biological factors that affect medicinal plant selection.ConclusionThe study of edaphological and climatological factors, on the one hand, and culture, on the other, can help us to understand why a plant is replaced by another one for the same purposes, either in the same or in a different area. In many cases, the cultural factor means that the use of a species is more widespread than its ecological distribution. This may also explain the presence of synonyms and polysemies which are useful for discussing ethnopharmacological data.

Christie complementary therapist wins national "public servant" award

To provide significant ethnopharmacological information on the plants used to treat digestive problems in the Navarra region of Spain.Information was collected using semi-structured, ethnobotanical interviews with 667 informants (mean age 72; 55.47% women, 44.53% men) in 265 locations. Monographs from the European Scientific Cooperative on Phytotherapy (ESCOP), the German Commission E, the World Health Organization (WHO), the European Medicines Agency (EMA), the European Pharmacopoeia (Ph. Eur.) and the Real Farmacopea Española (RFE) were used to confirm and validate the pharmacological actions for the reported uses of these plants. In cases when frequently reported plants were not covered by a monograph, a literature review was performed using a new tool from the University of Navarra: the UNIKA database.A total of 1214 pharmaceutical uses were reported by the informants of this study; these uses originated from 126 plants and 47 families and were mainly represented by Asteraceae, Lamiaceae and Rosaceae. The most frequently used parts of the plants were the inflorescences, followed by the flowered aerial parts and fruits. Thirty-three out of 126 plants (26%) and 322 of their identified 1214 popular uses (27%), have already been pharmacologically validated.The authors propose that four species should be explored and validated (Santolina chamaecyparissus ssp. squarrosa, Jasonia glutinosa, Jasonia tuberosa and Prunus spinosa) because these species are frequently mentioned and show promise for therapeutic treatments.

Volatile composition of Jasonia glutinosa D. C.

Using GC-MS the volatile composition of Jasonia glutinosa D. C., was studied by comparing two different methods for the isolation of a volatile fraction: distillation from the fresh plant in order to obtain the essential oil, and direct thermal desorption (DTD). Compared with essential oil extraction the main advantages of the DTD technique are the smaller sample amount required, and the increased range of volatile compounds which can be subsequently analysed by GC-MS.

Sesquiterpenes from Jasonia glutinosa: in vitro anti-inflammatory activity.

Four sesquiterpenes isolated from Jasonia glutinosa D.C. (Asteraceae), namely lucinone, glutinone, 5-epi-kutdtriol and kutdtriol, have been evaluated for their in vitro anti-inflammatory activity in cellular systems generating cyclooxygenase (COX) and 5-lipoxygenase (5-LOX) metabolites. None of the compounds assayed had a significant effect on leukotriene C4 (LTC4)-release from calcium ionophore-stimulated mouse peritoneal cells. However, the release of prostaglandin E2 (PGE2) by mouse peritoneal cells stimulated with calcium ionophore was inhibited by these compounds, although with less potency than the reference drug indomethacin (IC50=0.24 microM). The IC50 values of the active compounds were: lucinone 42.69 microM, glutinone 3.61 microM, 5-epi-kutdtriol 1.28 microM and kutdtriol 39 microM. Of the tested compounds, only glutinone (IC50=24 microM) showed a significant effect on thromboxane B2 (TXB2)-release induced by calcium ionophore in human platelets, although with less potency than the reference drug ibuprofen (IC50=1.27 microM).

Two new eudesmane alcohols from Jasonia glutinosa.

Two new sesquiterpene alcohols have been isolated from the aerial parts of Jasonia glutinosa D. C. The structure of these sesquiterpenes were characterized by 1D and 2D NMR techniques (DQCOSY. TOCSY, NOESY, HMQC and HMBC) as (11R)-eudesm-4-en-11,12-diol and (11R)-eudesmane-5alpha, 11,12-triol.

Volatile components obtained from the leaves of Jasonia glutinosa

The components of the essential oil and of the pentane extract of the leaves of Spanish wild growing Jasonia glutinosa were studied for the first time by GC-MS and GC with FID detector. The leaves of Jasonia glutinosa are poor in terpene and sesquiterpene hydrocarbons, and rich in their oxygen derivatives. The principal identified components of the essential oil are camphor (42.4%), endo-borneol (17.8%), α-terpineol (1.0%), nerolidol (4.2%) and T-cadinol (2.3%), and the principal identified components of the pentane extract are α-pinene (3.5%), camphor (38.3%), endo-borneol (12.1%), γ-selinene (1.0%), nerolidol (4.6%), bornyl formate (1.2%), nonacosane (1.0%) and hentriacontane (1.2%). The method used to extract these components from the plant affects the nature of the components obtained and their relative proportions.

Preliminary screening of antiprotozoal activity of Jasonia glutinosa aerial parts

As a part of a research on anti-protozoan agents, the acetonic extracts from Jasonia glutinosa aerial parts (Asteraceae) were tested in vitro against Entamoeba histolytica, Leishmania donovani (promastigote forms) and Trichomonas vaginalis. The 20% acetone extract was not effective at the assayed concentrations. The pure acetone extract was found to be active against Leishmania donovani and Entamoeba histolytica.

Two sesquiterpenoids, lucinone and glutinone, from Jasonia glutinosa

Two new sesquiterpenoids—lucinone (1) and glutinone (2)—isolated from the aerial parts of Jasonia glutinosa have been characterized by 1D and 2D NMR techniques. The complete structure of these sesquiterpene compounds have been determined as 5β,11,12-trihydroxy-iphionan-4-one and 2-[5′-(2′-oxopentyl)]-2-methyl-5-(1′-hydroxy-1′-methylethanol)-cyclohexanone, respectively.

Eudesmane alcohols from jasonia glutinosa

Three new sesquiterpene alcohols have been isolated from Jasonia glutinosa. Their structures were elucidated by spectroscopic methods and chemical correlations as (−)-[11 R]-4α,14-epoxyeudesm-11,12-diol, (−)-[11 R]-eudesm-4(14)-en-5β,11,12-triol and (+)-[11 R]-eudesm-4(14)-en-5α,11,12-triol and they are called α-epoxy kudtdiol, 5- epi-kudtriol and kudtriol respectively.

Taxonomic and nomenclatural notes on the genera Jasonia Cass, and Chiliadenus Cass. (Compositae)

Summary The starting-point for this research was the critical examination of a rupestrian chamaephyte gathered in Malta, which in the more recent local floras and in Italian floras is indicated as Jasonia glutinosa (L.) DC., species distributed in Spain, France, Balearic Islands, Lampedusa, Malta, Morocco. The doubt that it was not a well identified entity arose from the examination of numerous herbarium specimens, which has revealed the existence of some remarkable differences between the Maltese plant and Franco-Iberian one. The difference concern mostly the hairiness of the plant, the stem structure, the shape of the leaves, the disposition of the capitula in the scape, the involucral bracts and the achenes. The research for a valid basionym for these species has pointed out a confuse situation as far as nomenclature is concerned, wich has been solved, only by examinig the various types. The result has been that for the Franco-Iberian species the valid basionym is represented by Inula saxatilis Lam., w...