Jasmonic Acid Elicitation Research Articles

Jasmonic acid elicitation of anthraquinones with some associated enzymic and non-enzymic antioxidant responses in Morindaelliptica

Morinda elliptica cell cultures in an intermediary (G) medium treated with jasmonic acid (JA) elicitor, was used as a model system to understand the effects of elicitation on cell growth, anthraquinone (AQ) production, stress levels (lipid peroxidation and hydrogen peroxide (H 2O 2)), enzymic (catalase (CAT), ascorbate peroxidase (APO) and glutathione reductase (GR)) and non-enzymic antioxidant responses (total carotenoids, vitamin C and E). Cultures were elicitor-treated on day 12 with 50 or 100 μM JA, and harvested after 3 and 6 days of elicitation. The highest AQ content (23.6 mg g −1 DW) which was 2-fold to control but with cell growth (35 g l −1) 15% lower, was achieved with 100 μM JA. Prolonged elicitor treatment resulted in the highest level of lipid peroxidation (total MDA of 9 nmol g −1 FW) and H 2O 2 level (5.5 μmol g −1 FW). Total carotenoids, vitamin C and E were induced 200, 10 and 50%, respectively, after 6 days of elicitation. APO though induced between 100–300%, was only half the level in control, while GR was induced less than 100%. CAT on the other hand was reduced 100%.

Manipulating anthocyanin composition in Vitis vinifera suspension cultures by elicitation with jasmonic acid and light irradiation.

Jasmonic acid altered the accumulation of major anthocyanins in Vitis vinifera cell culture. Peonidin 3-glucoside content at day three was increased from 0.3 to 1.7 mg g(-1) dry cell wt while other major anthocyanins were increased by smaller increments. By day 14, the content of methylated and acylated anthocyanins (peonidin 3-p-coumaroylglucoside and malvidin 3-p-coumaroylglucoside) was 6.3 mg g(-1) DCW, in response to treatment with jasmonic acid, and comprising approximately 45% (w/w) of total anthocyanins. In comparison, the untreated control culture contained 1.2 mg g(-1) DCW which made up approximately 32% (w/w) of total anthocyanins. Light further enhanced anthocyanin accumulation induced by jasmonic acid elicitation. The content of peonidin 3-glucoside at day 3 was 6.6 mg g(-1) DCW, 22-fold higher than control cultures while the content in response to light irradiation alone was 0.6 mg g(-1) DCW. When a highly pigmented cell line was elicited with jasmonic acid total anthocyanins increased from 9.2 to 20.7 mg g(-1) DCW, but there was no change in the anthocyanin composition.

Jasmonic Acid Stimulates Taxane Production in Cell Suspension Culture of Yew (Taxus × media)

Cell lines of Taxus x media Rehd. were established via embryo and callus culture and grown on B5 medium containing 10 microM 2,4-D and 1 microM kinetin. Jasmonic acid (JA) was used to elicit taxane synthesis in a selected yew cell line. 100 microM JA was added 7 days after subculture. JA strongly increased taxane content in the cells and in the medium. At the time of the highest amount in elicited cultures, the enhancement of paclitaxel production was 19-fold and 4-fold in the cells and medium, respectively, compared to controls (non-elicited cultures). The time course of taxane accumulation in the cells and in the medium and the release of taxanes into the medium were not changed after JA elicitation.