This paper elaborates the issue about the importance of the national cultural values and organizational culture, through analysis and explanations of the American workers‘ culture in the organizational environment, observed through the prism of Hofstede‘s cultural dimensions in the USA/observed through the application of Hofstede‘s model in the context of the USA. In this regard, the leadership in American companies is also considered as a process that is significantly determined by the national and organizational cultural influences, by comparing the American and Japanese management styles, but also by perceiving the role of women in management in this country, that is, the issue of the "glass ceiling" concept. Each enterprise has a certain form of organizational culture that is currently dominant, but according to the requirements and impacts of various factors, it can be changed. The company always aims to recruit human resources that have developed a similar culture as that of the enterprise and have enough predispositions to accept it. There is often a comparison between companies in terms of their organizational culture. Thus, in some organizations, relations are highly formal and strictly hierarchically set, while in others, organizational culture is very open and informal. The findings of the cultural analysis of the American workers has shown that their culture is characterized by a high degree of flexibility and informality in relation to the manager-employee relationship, as well as in other aspects of the work; a high degree of individualism that is perceived through a strong appreciation of the achievement of individual initiative and achievement, self-esteem, autonomy and independence; tendency to achieve great successes; as well as freedom of expression and tolerance towards other ideas or opinions. At the same time, their management style is not based on too rigid norms and behavioral rules. Regarding the representation of women in management, although they are increasingly progressing and becoming part of the managerial staff, their number and influence still vary significantly.