Japanese Blood Research Articles

Blood-pool SUV analysis of 99mTc-galactosyl human serum albumin (99mTc-GSA) normalized by blood volume for prediction of short-term survival in severe liver failure: preliminary report.

This study evaluated the usefulness of SUV analysis of 99mTc-galactosyl human serum albumin (99mTc-GSA) scintigraphy including SUV analysis of the cardiac blood pool normalized by blood volume as a predictor of short-term survival in severe liver failure. We enrolled 24 patients with severe liver failure who underwent 99mTc-GSA scintigraphy and were admitted to the intensive care unit. Patients were divided into survival and non-survival groups at 7, 14, and 28days from the performance of 99mTc-GSA scintigraphy. From SPECT images we calculated SUVs of the cardiac blood pool, performing normalization for body weight, lean body weight, Japanese lean body weight, and blood volume and we calculated SUVs of the liver, normalizing by body weight, lean body weight, and Japanese lean body weight. We also calculated the uptake ratio of the heart at 15min to that at 3min (HH15) and the uptake ratio of the liver at 15min to the liver plus the heart at 15min (LHL15) from planar images of 99mTc-GSA scintigraphy. There were significant differences between the 7day survival and non-survival groups for all SUVs of the heart and the liver and HH15, for 14day survival groups in SUVs of the heart normalized by Japanese lean body weight and blood volume, and no significant differences between 28day survival groups for any SUVs, HH15, or LHL15. Although the difference was not significant, SUV analysis of the heart normalized by blood volume showed the highest value for the area under the receiver-operating-characteristics curve for both 7day and 14day survival. SUV analysis of 99mTc-GSA including SUV analysis of cardiac blood pool normalized by blood volume is of value for prediction of short-term survival in cases with severe liver failure.

Determination of the safety of Halomonas sp. KM-1-derived d-β-hydroxybutyric acid and its fermentation-derived impurities in mice and Japanese adults.

The use of halophilic bacteria in industrial chemical and food production has received great interest because of the unique properties of these bacteria; however, their safety remains under investigation. Halomonas sp. KM-1 intracellularly stores poly-D-β-hydroxybutyric acid under aerobic conditions and successively secretes D-β-hydroxybutyric acid (D-BHB) under microaerobic conditions. Therefore, we tested the safety of Halomonas sp. KM-1-derived D-BHB and the impurities generated during D-BHB manufacturing at a 100-fold increased concentration in acute tests using mice and daily intake of 16.0g D-BHB in Japanese adults for 12 weeks. In the mice test, there were no abnormalities in the body weights or health of mice fed the purified D-BHB or its impurities. In the Japanese adult test, blood parameters and body condition showed no medically problematic fluctuations. These findings indicate that Halomonas sp. KM-1 is safe and can be used for commercial production of D-BHB and its derivatives.

Incidences of new hepatitis B infection and anti-hepatitis B core-negative occult hepatitis B infection among Japanese blood donors in relation to anti-hepatitis B surface antigen levels.

A transfusion-transmitted hepatitis B virus (HBV) infection caused by blood only positive for anti-hepatitis B surface antigen (anti-HBs) was reported. Occult HBV infection (OBI) with sole anti-HBs among blood donors is an issue. The incidence of HBV infection among repeat blood donors was investigated with a detailed HBV infection phase, focusing on the influence of anti-HBs level. This study followed 3 435 653 donors for HBV DNA conversion over 4 years and 9 months. Infection phase was determined based on marker changes over DNA conversion. This study identified 115 hepatitis B surface antigen (HBsAg) conversions, 72 DNA-only conversions, and 15 DNA plus anti-hepatitis B core (anti-HBc) conversions among donors all negative for HBV DNA, HBsAg, and anti-HBc. Total incidence was 2.38/100 000 person-years (PY). None of these 202 new HBV infections arose in the group with anti-HBs titer ≥ 10 mIU/mL. In total, 30 anti-HBc-negative OBIs were identified (incidence; 0.35/100 000 PY); 7 showed typical secondary anti-HBs response, and 23 showed stable anti-HBc and anti-HBs levels at DNA conversion. The HBV infection-protective ability of anti-HBs ≥ 10 mIU/mL was reinforced. In addition to new infections, the blood donor population includes anti-HBc-positive- and negative OBI with immune reactions or abortive HBV infection.

The influence of dietary supplementation with fermented agro-industrial residue of faba bean on Japanese quail performance, immunity, gut microbiota, blood chemistry, and antioxidant status

Antibiotic overuse in poultry feeds has disastrous implications; consequently, long-term alternatives must be developed. As a result, the current study aims to assess the impact of Aspergillus niger filtrate (ANF) high in organic acids grown on agro-industrial residue of faba bean (AIRFB) on quail diet, as well as their influence on bird productivity, digestion, carcass yield, blood chemistry, and intestinal microbiota. A total of 240 Japanese quails (aged 7 d) were used in this study, divided equally among 5 experimental groups with 48 quails each. Group 1 (G1) received a basal diet without any ANF, group 2 (G2) received a basal diet supplemented with 0.5 mL ANF/kg diet, group 3 (G3) received a basal diet supplemented with 1.0 mL ANF/kg diet, group 4 (G4) received a basal diet supplemented with 1.5 mL ANF/kg diet, and group 5 (G5) received a basal diet supplemented with 2 mL ANF/kg diet. The performance parameters were monitored at 1 to 3, 3 to 5, and 1 to 5 wk. Adding ANF increased body weight at 3 and 5 wk, as well as body weight gain at 1 to 3, 3 to 5, and 1 to 5 wk, compared to the control diet. The ANF fed quails had the highest feed conversion ratio compared to the control group. The addition of ANF to the quail diet had no effect on the weight of the carcass, gizzard, heart, liver, giblets, or dressing; however, it did lower triglycerides, low-density lipoprotein, and very low-density lipoprotein while increasing high-density lipoprotein levels. The quail groups that received ANF had enhanced immunological indices such as IgG, IgM, IgA, and lysozymes. It also increased the levels of superoxide dismutase and total antioxidant contents, as well as catalase, and digestive enzymes such as protease, amylase, and lipase. However, it lowered the blood MDA levels compared to control. It has been demonstrated that the total gut microbiota, Escherichia coli, total coliforms, and the population of Salmonella are all reduced in ANF-fed quails. Histological examination of ANF quails' liver and intestinal sections revealed normal hepatic parenchyma, typical leaf-like intestinal villi, and comparatively short and frequently free lumina. In conclusion, Japanese quail showed improvements in performance, digestive enzymes, antioxidant indices, immunity, and capacity to reduce intestinal pathogenic bacteria after consuming diet supplemented with ANF.

Anti-monkeypox virus-neutralizing activities of human immunoglobulin manufactured between 1999 and 2021 and derived from donors in the United States and Japan.

In May 2022, the United Kingdom reported the first case of chained transmission of the monkeypox (mpox) virus without any known epidemiological links to west or central Africa. The monthly number of mpox patients currently has passed a peak and is declining globally, and infected patients include both non-vaccinated and vaccinated individuals. Herein, the virus-neutralizing (VN) activity against vaccinia viruses, which are considered to cross-react with the mpox virus, in the intravenous immunoglobulin (IVIG) lots derived from donors, including vaccinated Japanese populations, was evaluated to clarify the status of the Japanese blood donor population. VN titres against vaccinia and human mpox viruses in IVIG lots derived from donors in Japan and the United States manufactured between 1999 and 2021 and 1995 and 2001, respectively, were evaluated by neutralization testing. VN titres of IVIG derived from donors in Japan and the United States against vaccinia and mpox viruses showed a slowly decreasing trend between 1999 and 2021. VN titres are expected to decrease in the future since the percentage of vaccinated donors in the donor population seems to have decreased. Therefore, continuous monitoring of VN titres is required.

Characterization of mutations in hepatitis B virus DNA isolated from Japanese HBsAg-positive blood donors in 2021 and 2022

Missense mutations in certain small envelope proteins diminish the efficacy of antibodies. Consequently, tracking the incidence and types of vaccine-escape mutations (VEMs) was crucial both before and after the introduction of universal hepatitis B vaccination in Japan in 2016. In this study, we isolated hepatitis B virus (HBV) DNA from 58 of 169 hepatitis B surface antigen (HBsAg)-positive blood samples from Japanese blood donors and determined the nucleotide sequence encoding the small envelope protein. DNA from six (10%) of the samples had VEMs, but no missense mutations, such as G145R, were detected. Complete HBV genome sequences were obtained from 29 of the 58 samples; the viral genotype was A1 in one (3%), A2 in three (10%), B1 in nine (31%), B2 in five (17%), B4 in one (3%), and C2 in 10 (34%) samples. Tenofovir-resistance mutations were detected in two (7%) samples. In addition, several core promoter mutations, such as 1762A>T and 1764G>A, and a precore nonsense mutation, 1986G>A, which are risk factors for HBV-related chronic liver disease, were detected. These findings provide a baseline for future research and highlight the importance of ongoing monitoring of VEMs and drug resistance mutations in HBV DNA from HBsAg-positive blood donors without HBV antibodies.

Effects of varying dietary microalgae levels on performance, egg quality, fertility, and blood biochemical parameters of laying Japanese quails (Coturnix coturnix Japonica)

This experiment was carried out to investigate the nutritional value of Spirulina and Dunaliella (SD) combination levels (0, 0.5, 1.0, 1.5, and 2.0 g/kg) that affected the laying Japanese quail's efficiency, egg quality, fertility, and blood biological indicators. A total of 150 adult Japanese quails, aged eight weeks, were divided into five treatments at random, each consisting of 30 quails. There were five duplicates for every treatment, with two male and four female quails in each. Comparing the addition of various concentrations of a mixture of SD to the control treatment, the results showed no substantial rise in egg production, egg weight, or egg mass. When compared to the control group, final body weight (FBW) was improved with SD supplementation. The quails in control consumed more feed intake (FI) (p < 0.05), and they were different from the groups who got SD therapy in that they had a regular feed conversion ratio (FCR). The percentages of hatchability and fertility increased when SD was added to quail meals at up to 1.00 g/kg. When compared to the control quail, the quail supplemented with SD levels showed a non-significant rise in albumin%, yolk%, Haugh unit, and unit surface shell weight (USSW), as well as an increase in eggshell percentage and a drop in egg shape index (p < 0.05). Renal and hepatic enzyme functioning improved when SD was added to the diets. Additionally, lipid profile indicators were reduced by SD supplementation (except low-density lipoprotein-LDL). Moreover, compared to the control, incorporating SD led to a non-significant rise in immunoglobulin concentrations (IgG and IgM). In conclusion, adding SD to the diet can improve body weight, lipid profile, immunological response, and liver and kidney functions in Japanese quail.

Anemia-related subjective symptoms in the general adult population in Japan.

Objectives: Approximately 17% of Japanese women have hemoglobin concentrations less than 12 g/dL. Therefore, anemia prevention and early intervention are crucial public health issues in Japan. This study aimed to identify the symptoms and characteristics of anemic individuals in the general adult population by comparing survey responses of individuals with anemia and without anemia visiting blood donation centers. Materials and Methods: This cross-sectional study used self-administered questionnaires. Individuals who visited two Japanese Red Cross Society blood donation centers in Fukushima Prefecture, Japan were included. Hemoglobin levels were measured at blood donation, and the levels of 13 g/dL for men and 12 g/dL for women were defined as anemia. Results: Of the 857 individuals analyzed, 530 were men and 327 were women, of whom 19 (3.6%) and 12 (3.7%) had low hemoglobin levels, respectively. Logistic regression analysis was performed in men, and the results showed that "lightheadedness" (odds ratio [OR]=8.4) and "depressive symptoms" (OR=3.6) were significantly associated with hemoglobin levels. None of the evaluated items were significantly associated with hemoglobin levels in women. Conclusion: Among healthy Japanese men, those who exhibit lightheadedness and depressive symptoms have an increased risk of anemia. Lightheadedness and depressive symptoms may be indicative of undiagnosed anemia in men, which necessitates greater clinical attention.

The potential of Spirulina platensis to substitute antibiotics in Japanese quail diets: impacts on growth, carcass traits, antioxidant status, blood biochemical parameters, and cecal microorganisms

The development of antibiotic-resistant microorganisms prompted the investigation of possible antibiotic substitutes. As a result, the purpose of the current study is to assess the effect of dietary Spirulina platensis extract as an antibiotic alternative on Japanese quail (Coturnix japonica) growth, antioxidant status, blood parameters, and cecal microorganisms. There was a total of 150 Japanese quails used in this study, divided equally among 5 experimental groups (10 birds per group with 3 replicates): group 1 (G1) received a basal diet without any S. platensis extract, group 2 (G2) received a basal diet supplemented with 1 mL S. platensis extract/kg, group 3 (G3) received a basal diet supplemented with 2 mL S. platensis extract/kg, group 4 (G4) received a basal diet supplemented with 3 mL S. platensis extract/kg, and group 5 (G5) received a basal diet supplemented with 4 mL S. platensis extract/kg from d 7 until d 35. The results showed that compared to the control birds in G1, Japanese quail supplemented with 4 mL of S. platensis extract/kg of diet (G5) had significantly better live body weight, body weight gain, feed intake, feed conversion ratio, digestive enzymes, blood parameters, liver and kidney functions, lipid profile, antioxidant profile, immunological parameters, and cecal microorganism's count. There were no significant changes in the percentage of carcasses, liver, and total giblets among all the 5 groups. Only gizzard percentage showed a significant increase in G2 compared to birds in G1. In addition, intestinal pH showed a significant drop in G2 and G5 compared to birds in G1. After cooking the quail meat, the juiciness and tenderness increased as S. platensis extract levels increased, whereas aroma and taste declined slightly as S. platensis extract levels increased. Furthermore, when a high concentration of S. platensis extract was used, the lightness of the meat reduced while its redness and yellowness increased. The disk diffusion assay showed that S. platensis extract had significant antibacterial activity against Staphylococcus aureus, Listeria monocytogenes, Campylobacter jejuni, and Salmonella typhi, with inhibition zones ranging from 16 to 42 mm. This activity may be attributable to the volatile chemicals in S. platensis extract, of which Geosmin and 2-methylisoborneol are the primary components. In the diet of Japanese quails, it is possible to draw the conclusion that the extract of S. platensis can be utilized as a feed additive and as an alternative to antibiotics.

Anti-A and anti-B titers, age, gender, biochemical parameters, and body mass index in Japanese blood donors.

It has been reported that anti-A and anti-B (ABO antibody) titers decrease with age, but little is known about the association between ABO antibody titers and physiologic/biochemical parameters such as body mass index (BMI), gamma-glutamyl transpeptidase (GGT), and total cholesterol (T-Cho). We investigated the present situation of ABO antibody titers among healthy blood donors in Japan and the physiologic/biochemical factors that may be associated with changes in ABO antibody titers. Plasma from 7450 Japanese blood donors was tested for ABO antibody titers using ABO reverse typing reagents by an automated microplate system; donor samples were classified into low, middle, and high titers according to the agglutination results obtained with diluted plasma samples. Multivariate regression analysis was performed to analyze the association between ABO antibody titers and age, gender, biochemical parameters (alanine transaminase [ALT], GGT, globulin, T-Cho, and glycosylated albumin [GA]), and BMI according to the ABO blood groups. A significant correlation between ABO antibody titers and age/gender, except for gender in anti-A of blood group B donors, was observed. BMI showed significant but negative correlations with anti-A and anti-B (β = -0.085 and -0.062, respectively; p < 0.01) in blood group O donors. In addition, significant but negative correlations between GGT and T-Cho with anti-B of blood group A donors (β = -0.055 and -0.047, respectively; p < 0.05) were observed. Although differences existed among the ABO blood groups, ABO antibody titers seem to be associated with physiologic and biochemical parameters of healthy individuals.

Detection of early phase human T-cell leukemia virus type 1 and 2 infection with an improved confirmatory test.

Human T-cell leukemia virus type 1 (HTLV-1) is a blood-borne virus, and mandatory testing of donated blood for HTLV-1 antibodies has been adopted by Japanese Red Cross blood centers since 1986. A confirmatory line immunoassay was initiated in 2019 for individuals who were seroreactive in the screening test. This decreased the incidence of indeterminate individuals, however, donors with indeterminate results are not informed of their HTLV-1 seroreactivity and they can continue to donate blood. To clarify the characteristics of indeterminate line immunoassay results among Japanese blood donors. Of 759,259 blood donors in the Kyushu district of Japan, an area endemic for HTLV-1, 101 cases were classified as indeterminate by line immunoassay testing. We examined these cases using alternative secondary antibodies, anti-human-Ig (IgG/IgM/IgA) and -IgM antibodies, to detect the early phase of HTLV infection. Using anti-human-Ig and -IgM antibodies, HTLV infection status was confirmed in 37 individuals (HTLV-1-positive, 2; HTLV-positive, 27; HTLV-negative, 8). Among the remaining 64 indeterminate individuals, we identified one HTLV-2-infected 18-year-old female. A previous blood donation from this individual showed a negative anti-HTLV screening test result (signal-to-cutoff ratio=0.1). Therefore, this case was considered to be an HTLV-2 seroconversion case. These results indicate that the procedure for diagnosing HTLV infection should be reconsidered and that an accurate detection system for the early phase of HTLV infection is urgently needed for public health in Japan. Moreover, the issue of HTLV-2 infection needs a higher profile in Japan.

Decreased rate of blood donors with high ABO antibody titers in Japan and the underlying factors: Comparisons between 2010 and 2021

Background and ObjectivesPreviously (2007), it was reported that ABO antibody titers in Japanese blood donors had decreased significantly compared to 20 years before. Here we evaluated whether further decrease of antibody titers had occurred in recent years, and the potential factors associated with changes in antibody titers. Materials and MethodsSerum/plasma from random blood donors in 2010 and 2021 (2010: 3369, 2021: 5796 donors) was classified into low, middle, and high ABO antibody titers according to the reactivity of diluted serum/plasma (2.5-fold and 20-fold) by an automated microplate system. The rates of low/high titer in the two periods were compared. Logistic regression and age-gender-BMI subgroup analyses were conducted to identify the factors that contributed to changes in antibody titers. ResultsCompared to 2010, the rate of donors with high ABO antibody titers was decreased in 2021 for both anti-A and anti-B (anti-A, 2010: 23.8%, 2021: 19.3%; anti-B, 2010: 23.8%, 2021: 16.4%). In logistic regression analysis, age was found to significantly affect both anti-A and anti-B antibody titers (anti-A, adjusted odds ratio 0.36, 95% CI 0.31–0.41; anti-B, 0.42, 0.37–0.47), and BMI (0.82, 0.73–0.92) and other time-related factors (0.79, 0.71–0.88) significantly affect anti-B antibody titers. Subgroup analysis revealed decreased rate of high anti-B titers in the higher age group in 2021. ConclusionThe rate of high ABO antibody titers, especially high anti-B titers, was significantly decreased in 2021, and our results suggested an association with aging and obesity of blood donors as well as other time-related factors.

Neutralizing activity of intravenous immune globulin products against enterovirus D68 strains isolated in Japan

BackgroundEnterovirus D68 (EV-D68), belonging to Enterovirus D, is a unique human enterovirus mainly associated with common respiratory diseases. However, EV-D68 can cause severe respiratory diseases, and EV-D68 endemic is epidemiologically linked to current global epidemic of acute flaccid myelitis.MethodsIn this study, we measured neutralizing antibody titers against six clinical EV-D68 isolates in nine intravenous immune globulin (IVIG) products commercially available in Japan to assess their potential as therapeutic options for severe EV-D68 infection.ResultsSeven IVIG products manufactured from Japanese donors contained high neutralizing antibody titers (IC50 = 0.22–85.01 µg/mL) against all six EV-D68 strains. Apparent differences in neutralizing titers among the six EV-D68 strains were observed for all IVIG products derived from Japanese and non-Japanese blood donors.ConclusionsHigh levels of EV-D68–neutralizing antibodies in IVIG products manufactured from Japanese donors suggest that anti-EV-D68 antibodies are maintained in the Japanese donor population similarly as found in foreign blood donors. Apparent differences in neutralizing antibody titers against the six EV-D68 strains suggest distinct antigenicity among the strains used in this study regardless of the genetic similarity of EV-D68.

Evaluation of human T-cell leukemia virus in vitro diagnostics using plasma specimens collected in Japan

BackgroundIn vitro diagnostics (IVDs) for primary detection test/screening of human T-cell leukemia virus (HTLV) have recently been updated to new-generation products in Japan. In this study, the performance of these products was evaluated and discussed in terms of the usability of HTLV diagnosis in Japan.MethodsThe performance of 10 HTLV IVDs for primary detection test and confirmatory/discriminatory test was evaluated. Plasma specimens that had been declared ineligible for transfusion were provided by the Japanese Red Cross Blood Center.ResultsThe diagnostic specificity of the IVDs was 100% (160/160). Six sandwich assays resulted in all HTLV-1/HTLV-positive specimens being positive (46/46). On the other hand, one sandwich assay, IVD under development 2 (UD2), resulted in one HTLV-1-positive and one HTLV-positive specimen being negative (44/46, 95.7%). One indirect assay, HISCL HTLV-1, could not detect one HTLV-positive specimen (45/46, 97.8%), but the updated product, UD1, correctly detected it (46/46, 100%). Serodia HTLV-I, based on a particle agglutination assay, resulted in 44 of the 46 positive specimens, but could not detect two specimens (44/46, 95.7%). ESPLINE HTLV-I/II, based on an immunochromatography assay (ICA), was able to diagnose all specimens as positive (46/46, 100%).ConclusionsSix sandwich assays and an ICA demonstrated high diagnostic sensitivity and specificity and are recommended for use in HTLV diagnosis in conjunction with confirmatory/discriminatory test using the INNO-LIA HTLV-I/II Score.

Effects of special sports training on autonomic nervous regulation

BackgroundSports is one of the most traditional health rehabilitation programs in China. With wide applicability and low side effects, it plays a very good role in strengthening the body. In sports theory and practice, professional physical fitness training content can effectively inhibit the occurrence of corresponding diseases and has a good preventive effect on some diseases. Therefore, the regulating effect of physical training on human autonomic nervous function is evaluated through special sports training. The relationship between physical training and the human body and mind is verified through the sports professional training test, which provides effective support for the promotion and application of sports health therapy in the medical field.Subjects and Method160 students majoring in physical education in a sports college were selected as the research objects of this experiment. The physical functions of the experimental subjects were healthy, and the purpose and method of the experiment were clarified. The students were divided into an experimental group and a control group, with 80 students each. The autonomic nervous function of the experimental subjects was tested by the Japanese postural blood pressure reflex method, and the test was evaluated by the Japanese physiologist pig feeder’s neuromodulation method. The students in the experimental group had more than 8 hours of physical training every week, while the students in the control group were not allowed to do any physical training during the test time. The experimental period is half a year, and the parameters such as blood pressure, heart rate, lying-sitting blood pressure, heart rate, and other parameters of the experimental subjects are tested and recorded. All data in the experiment were statistically analyzed by Excel2016, and the data chi-square test value was calculated.ResultsThe data in Table 1 are the detection results of the autonomic nerve function characteristics of the two groups of people during exercise. The autonomic nervous system is the basic motor nerve unit of the human viscera. Its function is to regulate the secretion of internal organs and effectively maintain the blood pressure, heart rate, and respiration of the human body. The experimental group participating in the special sports training was significantly shorter than the control group in the recovery time of exercise center rate, respiration stabilization time, and blood pressure stabilization time (P &lt; 0.05).Table 1.Shows the detection results of autonomic nerve function characteristics during exercise in the two groupsGroup typeExercise heart rate recovery timeExercise breathing recovery timeExercise blood pressure recovery timeExperimental group (80)4.56 ± 1.255.23 ± 1.355.56 ± 1.12Control group (80)6.25 ± 1.358.26 ± 1.757.25 ± 1.48t8.21512.2618.144P&lt;0.05&lt;0.05&lt;0.05ConclusionsPhysical exercise is of great significance to the healthy development of people’s physical and mental health. Long-term professional sports training can effectively improve people’s various physical functions and ensure that people have a strong mental state. In the experimental test, the autonomic nerves of the students in the professionally trained experimental group were effectively regulated, and the recovery time of each functional test was shorter than that of the control group.

Cross-tissue correlations of genome-wide DNA methylation in Japanese live human brain and blood, saliva, and buccal epithelial tissues

Neuroepigenetics considers genetic sequences and the interplay with environmental influences to elucidate vulnerability risk for various neurological and psychiatric disorders. However, evaluating DNA methylation of brain tissue is challenging owing to the issue of tissue specificity. Consequently, peripheral surrogate tissues were used, resulting in limited progress compared with other epigenetic studies, such as cancer research. Therefore, we developed databases to establish correlations between the brain and peripheral tissues in the same individuals. Four tissues, resected brain tissue, blood, saliva, and buccal mucosa (buccal), were collected from 19 patients (aged 13–73 years) who underwent neurosurgery. Moreover, their genome-wide DNA methylation was assessed using the Infinium HumanMethylationEPIC BeadChip arrays to determine the cross-tissue correlation of each combination. These correlation analyses were conducted with all methylation sites and with variable CpGs, and with when these were adjusted for cellular proportions. For the averaged data for each CpG across individuals, the saliva–brain correlation (r = 0.90) was higher than that for blood–brain (r = 0.87) and buccal–brain (r = 0.88) comparisons. Among individual CpGs, blood had the highest proportion of CpGs correlated to the brain at nominally significant levels (19.0%), followed by saliva (14.4%) and buccal (9.8%). These results were similar to the previous IMAGE-CpG results; however, cross-database correlations of the correlation coefficients revealed a relatively low (brain vs. blood: r = 0.27, saliva: r = 0.18, and buccal: r = 0.24). To the best of our knowledge, this is the fifth study in the literature initiating the development of databases for correlations between the brain and peripheral tissues in the same individuals. We present the first database developed from an Asian population, specifically Japanese samples (AMAZE-CpG), which would contribute to interpreting individual epigenetic study results from various Asian populations.

Jesus Loves Japan: Return Migration and Global Pentecostalism in a Brazilian Diaspora, by Suma Ikeuchi

How do returning migrants relate do their ancestral culture? What are the cross-cultural and interreligious negotiations that occur? How do they make meaning of their in-between state in a new land? What happens when global Pentecostalism encounters a predominantly Buddhist and Shinto society? What role does religion, or more specifically Christianity, play in meaning-making and generating purpose in the lives of these returnees in a seemingly “foreign” and hostile land?In her seminal work, Jesus Loves Japan: Return Migration and Global Pentecostalism in a Brazilian Diaspora, Suma Ikeuchi (University of California, Santa Barbara) attempts to answer similar questions by looking at the day-to-day experience of a Brazilian Pentecostal community in Japan. This work is an ethnographic tour de force but also comprises a beautiful narrative of Nikkei (second-generation) Pentecostal resilience, hope, and integration in a familiar yet strange land.As a result of her personal encounter with world Pentecostalism, Ikeuchi takes us along her own journey of self-discovery, introducing readers to her experience living among Nikkei Brazilian immigrants navigating the two Js—Jesus and Japan—in the Brazilian Pentecostal Church of Missão Apoio Toyota (182). Her ethnographic and phenomenological study investigates their daily lives through such experiences as coffees, dinners, baby showers, birthday parties, soccer viewing gatherings, and funerals (28).The results are quietly impressive. Her overarching thesis is that Pentecostalism’s language of spiritual conversion embodied in the “born again” experience gives believers a sense of “transnational transcendence” in which they are now “members of ‘the culture of love’ which ostensibly overcomes narrow ethnonational affiliations” (183).In the first part, Ikeuchi describes the experience of Brazilian return migrants suspending their erstwhile Japanese identity. Returning to their ancestral homeland of Japan on the Nikkei-jin visa, these return migrants, or Nikkei, experience the phenomenon of “afastamento,” a “distancing” or feeling of disenchantment with the “ethnic aura” or “great pride of the Japanese race” (38). Ikeuchi masterfully shows how this plays out in the Nikkei encounter with typical Japanese customs such as Obentō and Shūdan Tōkō, and even the menial task of garbage disposal (66, 73).The second part shows how the church functions as a means of renewal for the Nikkei sense of meaning and identity in a foreign land. Ikeuchi shows how prayer, sermons, testimonies, and Bible studies allow believers to experience a “break” from the “temporal suffocation” of Japanese work life (91). This helps them produce a “culture of love” and “create a new sense of citizenship” in a land that formerly appeared hostile but now has more potential to be Christian “than their former land (Brazil)” (108). Ikeuchi introduces the valuable category of love, which goes beyond simplistic reductionist attempts to reduce conversion experience to mere causal materialist processes.The third section explores how this renewed sense of identity in Christ offered by Pentecostalism affects the intercultural exchanges between Nikkei and Pentecostal identities. Ikeuchi argues that even though Nikkeis occupy a contested space in Japanese society, their view of Christian “kinship” enables them to overcome the sense of inferiority and distancing they experience in a society grounded on notions of “Japanese blood” (122).In her conclusion, Ikeuchi notes that this renewed sense of identity allows Nikkeis to reconceptualize the meaning of return migration. Nikkei Pentecostals see themselves as pilgrims hoping for their true “spiritual” homeland. Nostalgia for Brazil is replaced with spiritual vocation for Japan. The tensions of transnational mobility and contested citizenship give way to the hope that they have a special calling. After all, “Jesus ama o Japão” (186).In sum, Jesus Loves Japan is a heartwarming but nevertheless rigorous scientific study that captures the struggles, pains, faith, hopes, desires, and love of Nikkei Pentecostals. It also serves as an excellent companion to others like Christina Moreira da Rocha’s Zen Buddhism in Brazil: Japanese or Brazilian? It contributes to Pentecostal studies by showing the diffusion of Pentecostalism in a predominantly Buddhist and Shinto culture. For East Asian studies, it sheds light on minority and unrepresented voices. Suma Ikeuchi introduces readers to a world where faith, hope, and love help migrant community members reimagine their identities in order to survive in a difficult environment.

일본인의 피는 다른가?

The blood type discovered in 1901 was used as a sign of racial characteristics, as it was found that it had hereditary in 1910. In conjunction with eugenics, there was a tendency to perceive that the difference between Westerners‘ blood type characteristics was a criterion for determining superiority and inferiority, leading to research that divided Japanese and their neighbors. This paper examined the ‘scientific’ movement to define the Japanese through blood type as well as the popularity of eugenics discourse in Japan since the late 1920s. Tanemoto Furuhata, a researcher who represented Japanese blood type studies before and after the war, surveyed 300,000 people and measured the results with the biochemical race index made in Germany. Furuhata used the results to emphasize the differences between the surrounding peoples, ignoring the big differences from the West, and argued that the Japanese blood was “different” from the neighbours.&#x0D; On the other hand, based on the biochemical race index and the research results of Tanemoto Furuhata, Takeji Furukawa created blood type and personality discourse, while at the same time creating an argument that blood type and personality discourse could measure temperament in a group, that was, an ethnic unit. This paper found that his blood type and personality discourse was closely related to blood type and racist discourse, and that personality discourse was also used as a mechanism to overcome Japanese enthusiasm in racist discourse and discriminate Japanese from other ethnic groups. Takeji Furukawa's blood type and personality discourse had been criticized by academia, but on the contrary, it had received enthusiastic support from the public. It can also be seen that academia in Japan before the war also continued to study blood types with such personality discourse in mind.&#x0D; Although Tanemoto Furuhata and Takeji Furukawa had different specialties in forensic science on one side and psychology and pedagogy on the other, the two continued to have something in common in terms of trying to find the characteristics of the Japanese people through blood types. The two used common statistical data, and this paper found that they were similar in the direction of trying to reveal the existence of a unique blood type for Japanese based on a nationalistic perspective in analyzing the data.

Effects of Different Concentration of Copper on Performance, Immunity and Carcass Traits in Broiler Japanese Quails.

In this study, the effects of dietary copper supplements on broiler Japanese quail growth performance, immune response, blood biochemistry, and carcass quality were examined. Two copper sources (copper sulphate-CuS, and copper methionine-CuM), each at five distinct dietary dosages of 5, 10, 15, 100, and 150mg/kg, were used. A total of 280 (10 × 4 × 7) day-old quail chicks of uniform body weight were randomly distributed into 10 treatments with 4 replicates each and having 7 chicks in each replicate. In comparison to CuS-supplemented diets, CuM-supplemented diets (100mg Cu/kg diet) considerably (P ≤ 0.01) increased body weight gain and improved feed conversion ratio (FCR). In the 150-mg CuM/kg diet, the cell-mediated immune response (foot web index to PHAP) was considerably (P ≤ 0.01) greater. The humoral immune response (HA titre to SRBC) was substantially (P ≤ 0.01) lower with CuS-supplemented meals than with CuM-supplemented diets. When compared to CuS source, the weight of the bursa and spleen from CuM source was considerably (P ≤ 0.01) higher. The 100- and 150-mg CuM/kg diets considerably (P ≤ 0.01) reduced serum cholesterol levels. Thus, it may be concluded that dietary supplementation of copper methionine as a source of Cu @ 100mg Cu/kg diet to broiler Japanese quails was more effective in improving growth performance, immunological response, carcass quality features, and serum cholesterol reduction.

Genetic background of anti-Xga producers in Japanese blood donors.

The Xg blood group is composed of two antigens, Xga (XG1) and CD99 (XG2 and MIC2). The XG and CD99 are homologous genes located on pseudoautosomal region 1 of the X and Y chromosomes. The expressions of Xga and CD99 are co-regulated by a single nucleotide polymorphism (rs311103) in the GATA-1 binding region. Another mechanism of the Xg(a-) phenotype is the genomic deletion of approximately 114 kb, including the XG gene. Anti-Xga seems to be naturally occurring by detection in males who have never been transfused. In this study, we identified 23 anti-Xga producers among 580,115 donors (0.004%). Additional 12 anti-Xga producers were also identified from a separate cohort. All 35 anti-Xga producers were male. Genomic DNA was obtained from 34 of 35 producers, and all 34 producers were confirmed to carry the XG-gene-deficient allele (XGdel). The breakpoints of all 34 producers were identical. The XGdel was also identified in 12 non-producers of anti-Xga among 860 donors who have no antibodies against RBCs, and the breakpoints were also identical with the anti-Xga producers. Our results will serve as the basis for a more complete understanding of Xg blood group polymorphisms.