Vitamin D is a nutrient necessary for conditioning athletes. This cross-sectional study aimed to determine the nutritional status of vitamin D in farm-league professional baseball players to examine vitamin D requirements. This study included 35 players enrolled in farm team A of the Nippon Professional Baseball Organization in the spring of 2022. The survey items included anthropometric measurements, serum 25-hydroxyvitamin D [25(OH)D] levels as an indicator of vitamin D nutritional status, a simple questionnaire for predicting vitamin D deficiency in Japanese adults, mean daily sun exposure duration, and body surface area (BSA). The serum 25(OH)D level was 29.8±1.8 ng/mL, and 17 (49%) participants were vitamin D deficient (<30 ng/mL). The serum 25(OH)D levels showed positive correlations with body height (r=0.459, P<0.01), fat-free mass (r=0.347, P<0.05), muscle mass (r=0.341, P<0.05), and BSA (r=0.434, P<0.01). Furthermore, the consumption frequency of fish with high vitamin D levels in regular diets, BSA, and body fat mass were identified as predictors of the serum nutritional status of vitamin D. Athletes engaged in outdoor sports, such as the study participants, had inadequate vitamin D levels. In addition, the frequency of consumption of fish with high vitamin D levels in regular diets, BSA, and body fat mass were identified as predictors of the serum nutritional status of vitamin D. Further studies of the relationship between vitamin D supplementation and performance are needed.
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