We present a second short list of already identifi ed lichens collected during OPTIMAITER to Tunisia in 2014. The lichens were collected in 20 sampling sites in the northern part of the country. We report on ecological and distributional data related to 45 taxa, 11 of them were not included in the published checklist of lichens of Tunisia and further papers related to the diversity of lichens of Tunisia (Calicium glaucellum, Catapyrenium daedaleum, C. psoromoides, Gyalecta jenensis, Haematomma ochroleucum, Lecania sylvestris, Lecanora argentata, Parmotrema stuppeum, Physcia phaea, Ramalina subgeniculata, and Rinodina pyrina).