Marche Region Of Italy Research Articles

Effects of a supplemented diet containing 7 probiotic strains (Honeybeeotic) on honeybee physiology and immune response: analysis of hemolymph cytology, phenoloxidase activity, and gut microbiome

BackgroundIn this study, a probiotic mixture (Honeybeeotic) consisting of seven bacterial strains isolated from a unique population of honeybees (Apis mellifera ligustica) was used. That honeybee population was located in the Roti Abbey locality of the Marche Region in Italy, an area isolated from human activities, and genetic contamination from other honeybee populations. The aim was to investigate the effects of this probiotic mixture on the innate immunity and intestinal microbiome of healthy common honeybees in two hives of the same apiary. Hive A received a diet of 50% glucose syrup, while hive B received the same syrup supplemented with the probiotics, both administered daily for 1 month. To determine whether the probiotic altered the immune response, phenoloxidase activity and hemolymph cellular subtype count were investigated. Additionally, metagenomic approaches were used to analyze the effects on gut microbiota composition and function, considering the critical role the gut microbiota plays in modulating host physiology.ResultsThe results revealed differences in hemocyte populations between the two hives, as hive A exhibited higher counts of oenocytoids and granulocytes. These findings indicated that the dietary supplementation with the probiotic mixture was safe and well-tolerated. Furthermore, phenoloxidase activity significantly decreased in hive B (1.75 ± 0.19 U/mg) compared to hive A (3.62 ± 0.44 U/mg, p < 0.005), suggesting an improved state of well-being in the honeybees, as they did not require activation of immune defense mechanisms. Regarding the microbiome composition, the probiotic modulated the gut microbiota in hive B compared to the control, retaining core microbiota components while causing both positive and negative variations. Notably, several genes, particularly KEGG genes involved in amino acid metabolism, carbohydrate metabolism, and branched-chain amino acid (BCAA) transport, were more abundant in the probiotic-fed group, suggesting an effective nutritional supplement for the host.ConclusionsThis study advocated that feeding with this probiotic mixture induces beneficial immunological effects and promoted a balanced gut microbiota with enhanced metabolic activities related to digestion. The use of highly selected probiotics was shown to contribute to the overall well-being of the honeybees, improving their immune response and gut health.

Life cycle assessment of open field sea fennel production in central Italy

Sea fennel is a minor crop of emerging socioeconomic importance in the Mediterranean region. Despite its potential, there has been no assessment of its environmental impacts to support its perceived sustainability for broader promotion. Using a life cycle assessment (LCA), we evaluated the environmental performance of sea fennel production in an open field. A cradle-to-farm gate assessment was conducted using a functional unit (FU) of 1 kg of fresh sea fennel at the farm gate. The system boundaries encompassed the nursery, cultivation, and waste management phases. Primary data was collected from farms in the Marche region of Italy. The environmental impacts were assessed using the Environmental Footprint (EF) 3.0 midpoint life cycle impact assessment method. From the results, the primary hotspot input was plastic mulch, with a relative contribution of over 50% across most impact categories. Increasing the recycling rate from 30% to 70% substantially improved the environmental performance of sea fennels, reiterating the need for further investment in plastic recycling. This study provides insights into the environmental sustainability of sea fennel production. Findings demonstrate that sea fennel cultivation offers a promising path toward sustainable biomass production, underscoring the prospects for its commercial exploitation.

Debt financing of SMEs: The certification role of R&D Subsidies

This study investigates the (unintended) effects of public subsidies for research and development (R&D) on the debt financing of small and medium-sized enterprises (SMEs) by examining a public program implemented in the Marche region of Italy during the 2005–2012 period. The study combines matching methods with a difference-in-differences estimator to examine whether receiving public subsidies affects total indebtedness and the structure and cost of debt of awarded firms. The results indicate that R&D subsidies are associated with a modification of firm debt structure (especially for young, high-tech, and risky firms) in favor of long-term maturities and help firms limit the average cost of debt. Subsidies also foster the use of bank financing and reduce trade debt, but they do not affect the overall level of debt. Taken together, these findings suggest that the public funding of SME innovation projects plays a certification role in the access to external financial resources for firms receiving subsidies.

Point prevalence survey of antibiotic use and healthcare-associated infections in acute care hospitals: a comprehensive report from the Marche Region of Italy

Healthcare-associated infections (HAIs) and antimicrobial resistance (AMR) are serious health challenges. Point prevalence surveys (PPSs) are valuable tools for monitoring HAIs and AMR. To describe results of the ECDC PPS 2022 dealing with the prevalence of HAIs, antimicrobial consumption, and associated factors, in acute care hospitals. The survey was performed in November 2022 in 14 hospitals according to the protocol proposed by the European Centre for Disease Prevention and Control. Multilevel logistic regression was performed using geographical area/hospital type as cluster variable to evaluate the factors independently associated with HAIs and antibiotics. The point prevalence of HAIs was 7.43%. Patients hospitalized for longer periods were more likely to have an HAI as well as those aged 15-44 years, with a rapidly fatal disease, intubated, and with one or two devices. Antibiotics prevalence was 47.30%. Males, unknown McCabe scores, minimally invasive/non-National Healthcare Safety Network (NHSN) surgery, patients with HAIs, hospitals with a higher alcohol hand-rub consumption, hospitals with a greater number of IPC personnel, geriatric wards, and hospitals with 300-600 beds were more likely to be under antimicrobial therapy. This PPS provided valuable information on the prevalence of HAIs and antimicrobialconsumption and variables associated. The high prevalence of HAIs highlights the need for improved infection control measures.

Assessment of speech intelligibility in scholar classrooms by measurements and prediction methods

According to Italian regulation, the Ministerial Decree of 11 October 2017 about Environmental Criteria, reference values for acoustic indoor quality descriptors in public buildings are imposed. Regarding school environments, indoor acoustic quality targets refer to reverberation time, clarity, and speech intelligibility, whose representative acoustic descriptor is the speech transmission index (STI). This paper presents pyeSTImate, a Python-based tool for speech transmission index prediction in lecture rooms. The tool returns fully simulated results from the dimensions and material characteristics of classrooms with parallelepiped geometry and without limitations in size. Extensive experiments have been conducted with different simulation methods, evaluating the accuracy by comparison with in situ measurements selected from primary, secondary, and university classrooms in school buildings of the Marche Region in Italy. The combination of simulated speech transmission indexes with a prediction method based on an artificial neural network has also been evaluated. The analysis of the performance demonstrates the computational robustness of the tool that enables its use for the analysis of existing rooms, as well as for the renovation and design of new spaces.

On the Use of White Marbles in Roman Forum Sempronii (Fossombrone, Marche, Italy): An Overview of the Archaeometric Data on Architectural Elements

This paper presents the results of the characterisation, identification, provenance determination, and quantification of the white, greco scritto-like and polychrome marbles used for architectural purposes at Forum Sempronii, a Roman town in the Marche region of Italy. Samples of five fragments of white marble and one fragment of a greco scritto-like marble have been studied using mineralogical and petrographic observations and stable isotope ratio analysis (SIRA) of carbon and oxygen. Polychrome marbles were macroscopically identified by comparison with reference samples and available image databases. Analytical results of the white and greco scritto-like marbles identified the material as coming from the quarries of Carrara (Luni, Italy) and Proconnesos (Marmara, Saraylar, Turkey) for white marble, and of Ephesos-Hasançavuslar for greco scritto-like marble. Further, at least eleven imported polychrome stone varieties were distinguished. The study clearly illustrates that Forum Sempronii had access to the main marble trade networks of the Roman Mediterranean. The early attestation of Proconnesian marble at the town’s augusteum (i.e., Augustan date) is unique for central Adriatic Italy.

Quick Report on the ML = 3.3 on 1 January 2023 Guidonia (Rome, Italy) Earthquake: Evidence of a Seismic Acceleration

This paper investigates possible anomalies on the occasion of the ML = 3.3 earthquake, which occurred on 1st January 2023 close to Guidonia Montecelio (Rome, Italy). This earthquake followed another very close event recorded on 23 December 2022 with a magnitude ML = 3.1 (epicentral distance of less than 1 km). Seismological investigations clearly show an acceleration of seismicity in the preceding six months in a circular area of about a 60 km radius. Two conclusions coming from the time-to-failure power law fitting to the cumulative Benioff strain curve are the most probable: the ML3.3 of 1 January 2023 is the mainshock of the seismic sequence, or an incoming earthquake of a magnitude of about 4.1 provides a slightly better fit (higher determination coefficient) of the seismic data. Further investigations are necessary to assess whether the accumulated stress has been totally released. No atmospheric anomalies related to this seismic activity have been identified, even if some SO2 emissions seem to be induced by the tectonic and volcanic sources in the south Tyrrhenian Sea. Swarm satellite magnetic data show about 20 anomalous tracks six months before the Guidonia earthquake. In particular, on 16 December 2022, anomalous oscillations of the east component of the geomagnetic field are temporally compatible with the seismic acceleration, but other sources are also possible. Other anomalous magnetic signals are more likely to be associated with the ongoing seismic activity offshore of the Marche region in Italy (the strongest event up to now is Mw = 5.5 on 9 November 2022 close to Pesaro Urbino).

Increasing ventilation reduces SARS-CoV-2 airborne transmission in schools: A retrospective cohort study in Italy's Marche region.

While increasing the ventilation rate is an important measure to remove inhalable virus-laden respiratory particles and lower the risk of infection, direct validation in schools with population-based studies is far from definitive. We investigated the strength of association between ventilation and SARS-CoV-2 transmission reported among the students of Italy's Marche region in more than 10,000 classrooms, of which 316 were equipped with mechanical ventilation. We used ordinary and logistic regression models to explore the relative risk associated with the exposure of students in classrooms. For classrooms equipped with mechanical ventilation systems, the relative risk of infection of students decreased at least by 74% compared with a classroom with only natural ventilation, reaching values of at least 80% for ventilation rates >10 L s-1 student-1. From the regression analysis we obtained a relative risk reduction in the range 12%15% for each additional unit of ventilation rate per person. The results also allowed to validate a recently developed predictive theoretical approach able to estimate the SARS-CoV-2 risk of infection of susceptible individuals via the airborne transmission route. We need mechanical ventilation systems to protect students in classrooms from airborne transmission; the protection is greater if ventilation rates higher than the rate needed to ensure indoor air quality (>10 L s-1 student-1) are adopted. The excellent agreement between the results from the retrospective cohort study and the outcome of the predictive theoretical approach makes it possible to assess the risk of airborne transmission for any indoor environment.

Frasassi Caves and Surroundings: A Special Vehicle for the Geoeducation and Dissemination of the Geological Heritage in Italy

One of the most fascinating aspects of the work of geologist lies in knowing how to read the physical landscape as an expression of the geological and geomorphological phenomena that shaped the Earth’s relief over time. The necessity to disseminate these vast areas of knowledges and skills starts from here, with the aim to enhance the concept of geodiversity and to raise awareness for its protection and promotion. This work aims to share some examples of projects realized following the subscription of agreement between different public authorities in the Apennine territory of Marche Region in Italy, such as the Geology Section of the University of Camerino (MC), “Consorzio Frasassi” (which manages the underground karst complex of Frasassi caves) and municipality of Genga (AN), where Frasassi caves are located. Thanks to this partnership, our research group realized didactic geological notebooks for school groups visiting the caves and interactive museum laboratories in 3D, showing the geological evolution of the area. This sharing of knowledge will contribute to educate communities about the importance of the geological heritage. At the same time, this project can serve as motivation to establishing the same type of collaborations in those territories where similar projects can be replicated.

Theatre without theatres: Investigating access barriers to mediatized theatre and digital liveness during the covid-19 pandemic

In each stage of the Covid-19 pandemic, we have witnessed initiatives that, through digital technologies, have attempted to ensure the presence of theatre and to nurture the relationship with audiences. Our research asks which entry barriers to the artistic field have been strengthened or weakened by implementing theatre initiatives for online audiences and how these initiatives have affected the regional performing arts scene. The study consists of three parts. In the first part, analysis of Italian calls for digital performance projects was carried out to investigate the institutional construction of beneficiaries and imagined audiences. In the second part, we analysed the case of the digital-theatre season MPA - Marche Palcoscenico Aperto. The MPA project provided funding for artists from the Marche region in Italy to realize online performances between February and May 2021. Eleven focus groups were conducted with 41 of the 60 participating companies. In the third phase, four in-depth interviews were conducted with the project's organisers. Findings show how the increased dependence of theatre artists on the artistic system imposed by Covid has simultaneously produced an increase in the collective awareness of the artistic class, but also a stronger distinction between professionalism and amateurism.

Application of a Non-Invasive Technique for the Preservation of a Fortified Masonry Tower

The Cultural heritage spread all over the World needs to be preserved with systems that do not compromise its architectural and historical value. Nowadays, the most advanced technology available is the ambient vibration test. It allows to obtain the current frequencies, modal shapes, and damping of structures, without being invasive. The comparison between different monitoring campaigns and their use in combination with Finite Element models can give an insight into the state of structures’ health. This paper presents two ambient vibration tests performed on a fortified masonry tower in the Marche region of Italy, carried out after one year each other, with different temperature and humidity conditions. To extract the structure’s dynamic parameters both a time and a frequency domain approaches were used. The comparison between the parameters obtained during the two experimental campaigns showed similar frequencies and modal shapes underlining that no damage occurred and that the dynamic response of the tower does not suffer temperature and humidity variations. In addition, the steps carried out for a first attempt manual calibration of the tower’s Finite Element model are shown. The match between the numerical model and the experimental data is evaluated through the absolute frequencies’ errors and the Modal Assurance Criteria between the modal vectors. The calibrated numerical model can be used for future and accurate assessment of the tower’s structural capacity.

Exploring motivations behind the introduction of tourist accommodation taxes: The case of the Marche region in Italy

The accommodation tax is one of the most common forms of tourism taxation to tackle tourism-related negative externalities, usually administered at sub-national level by municipalities. Although widely applied, the accommodation tax is not unanimously adopted, which makes it particularly interesting to investigate what encourages municipalities to rely on it. By using random effects panel logit and probit regression models on a set of Italian municipalities, this paper contributes to the scant literature that explores the issue and shows differences from similar studies. Specifically, our findings suggest that the probability of introducing a tourist accommodation tax is related to the size and fiscal conditions of the municipality, occupancy of tourist accommodation establishments, and electoral disproportionality. Furthermore, as our results reveal that strategic interactions among municipalities within the same province play a role, the paper suggests to consider the accommodation tax as a strategic tool in competition among tourist destinations.

Identification of Monilinia spp. from stone fruits in the Marche region of Italy

ISHS V International Symposium on Postharvest Pathology: From Consumer to Laboratory-Sustainable Approaches to Managing Postharvest Pathogens Identification of Monilinia spp. from stone fruits in the Marche region of Italy

Two-Source Capture-Recapture Method to Estimate the Incidence of Acute Flaccid Paralysis in the Marches Region (Italy).

A combination of high infant immunization coverage and surveillance of acute flaccid paralysis (AFP) cases, plays a critical role in polio eradication. This study aimed to estimate the incidence of AFP, to evaluate the completeness of AFP ascertainment during the years, age groups and gender, and to define the main associated diagnosis among children aged under 15 in the Marches region of Italy. Analysis was performed on data from the active AFP surveillance system and the hospital discharge records in the 2006–2014 period. The two-source capture-recapture method was applied. After cross-validation, 30 AFP compatible conditions as defined by the WHO were identified, with an incidence estimate of 1.91 cases of AFP per 100,000 children under 15 years (95% CI = 1.4–2.6/100,000). Guillain–Barrè syndrome was the most common diagnosis. A significant difference (p < 0.05) has been registered in the estimated probability of case ascertainment in females. The reasons for the lower reporting of cases in females are unknown. Specific research and the implementation of a more sensitive surveillance system are essential in verifying potential inequalities and to succeed in the polio eradication initiative.

Reduction in acute coronary syndromes during coronavirus disease 2019 global pandemic: data from the Marche region of Italy.

During the novel severe acute respiratory syndrome coronavirus 2 (coronavirus disease 2019) pandemic, a worldwide reduction in total acute coronary syndrome (ACS) has been reported. In early 2020, Italy became the most affected country and national lockdown was declared early on in March. We described trends in ACS from all the Marche coronary catheterization laboratories (CCL) during the global pandemic. Retrospective study of all consecutive patients admitted to the four regional CCL. The coronavirus disease 2019 period (20 February 2020 to 15 April 2020) was compared with the interyear control period (1 January 2020 to 19 February 2020) and to the intrayear control period (20 February 2019 to 15 April 2019). All patients with an initial diagnosis of ACS were included in the analysis, and further stratified into ST-segment elevation myocardial infarction (STEMI) and non-STEMI (NSTEMI)/unstable angina. A total of 1239 patients were enrolled. Daily incidence of ACS was 6.1, 6.3 and 4.5 for the interyear control period, the intrayear control period and the case period, respectively. There was no difference in overall STEMI daily incidence while NSTEMI/unstable angina fell from 3.6 and 3.3-1.8 during the case period (P = 0.01). Incidence rate ratios were significantly lower when the case period was compared with the intrayear control period (incidence rate ratios: 0.49, 95% confidence interval 0.41-0.59, P = 0.001) and the interyear control period (incidence rate ratios: 0.67, 95% confidence interval 0.50-0.90, P = 0.008). During the global pandemic there was a significant reduction in total ACS and NSTEMI in the Marche region. Unlike previous reports, there was no difference in overall access to CCL for STEMI during the same period.

What’s smart about smart specialization – a new EU innovation strategy or more of the same?

Purpose The aim of this paper is to outline what the intended benefits the smart specialization strategy (S3) is meant to create, and through what policy measures; that is, to shed light over what underpinnings S3 is based on, and if the measures based on these can affect the relations between “academia, businesses, and local authorities” – where the public and the private actors might have partly overlapping interests, but with different needs and rationales. Design/methodology/approach The research design of this paper is based on the industrial marketing and purchasing network approach, that is, the empirical observation that business exchange has a content, which affects and gives imprints on the actors engaged in the exchange. To determine whether the S3 strategy in general, and in the two investigated regions in particular, can affect the embedding of innovations in using, producing and developing settings, and if so how, this study applied the actors–resources–activities model. In addition to investigation of the S3 strategy in general, two case studies were conducted, one each in two European Union regions with rather different business and academic research characteristics: the Marche region in Italy and the Uppsala region in Sweden. Findings The S3 measures rest on the judgement of which “domains” to support can be made by policy actors without deeper analysis of how the assumed firms representing these domains are related in terms of how resources are combined and activated. Instead, the S3 policy analysis is based on local policy organizations desk table investigations of what appears as innovative. Hence, in practice, the key S3 measure is still to transfer knowledge from the public to the private sector. This entails that support in terms of how to create change in established resources interfaces, which is a main source of innovation to which both established and emerging localized firms are related, remains out of policy sight. Originality/value The ambition with this paper is to discuss what changes S3 – with the ambition to develop and match academic research to business needs – implies and what underpinnings it is resting on. Hence, the focus is directed to what new types of policy arrangements are supposed to result in what types of benefits – and last but not least, the ability for these to interfere with businesses which are interconnected across spatial borders.

Odontogenic tumours: a 25-year epidemiological study in the Marche region of Italy.

Epidemiological data of odontogenic tumours (OT) are conflicting, with significant differences among the countries. This study aims to evaluate incidence and prevalence of OTs in the Marche population in a period of 25years, according to 4th Edition of WHO Classification. In this study, only patients of Marche region treated for OTs were considered. Data were retrieved from Institute of Pathology, Marche Polytechnic University, Italy. Because this is the only tertiary referral centre for Head and Neck pathology within Marche region, the patient sample could be considered well representative of this area. From each case, age, sex, site, diagnosis and relapses were recorded. Overall, 100 patients were treated for OTs from 1994 to 2018 in Marche region. The annual incidence rate ranged from 0.13 to 0.39 per 100,000, while life prevalence was 6.50 per 100,000. Mean age of onset for primary OTs was 49.7 ± 20.1years. Twenty-seven patients developed recurrences, showing a mean age of 54 ± 19.7years and a mean recurrence time of 51.2 ± 34months. This is the first epidemiological study on OTs in Italian population according to 4th Edition of WHO Classification. Although limited in their retrospective nature, these findings could accurately estimate epidemiology of OTs in Italy.

Mangiare a scuola: un’indagine in alcune scuole italiane

The field research involved a survey in the contexts of school canteens, with the aim of identifying methods and good practices in school catering. 23 schools in the Marche region of Italy were involved in the survey, and the research reached 600 pupils, 81 teachers and 48 canteen operators. The research was designed and carried out by a group of 23 students from the course of Educational Psychology of the University of Macerata, with a survey methodology that saw a desk research on the Italian regulations dedicated to the school catering sector, the use of a structured observation checklist for canteens, the administration of semi-structured interviews with stakeholders. The analysis of the data describes a fragmented framework in the organization of school canteens, which, from a regulatory point of view, respect the parameters and rules of school catering, from the point of view of the educational approach to food have different characteristics. The research seems to confirm the lack of a common approach to food education, affirmed by the Ministry of Education itself in a national research on school catering (MIUR, FoodEdu 2018), highlighting strengths and weaknesses on which to leverage on aim to pursue the goal of a programmed and widespread and high quality nutrition education in all schools in Italy. As an answer to the issues raised from the data harvesting, we propose a food education project called Edueat - promoted by the training company Laboratorio delle Idee S.r.l. and scientifically supervised by the University of Macerata. The project aims to promote food education through the use of the senses, play and skills development, within the framework of the major theoretical references of Developmental and Educational Psychology.

Antioxidant and Anti-Inflammatory Properties of Nigella sativa Oil in Human Pre-Adipocytes.

The oil obtained from the seeds of Nigella sativa L. (N. sativa), also known as black cumin, is frequently used in the Mediterranean area for its anti-inflammatory, anti-oxidant, and anti-cancer activities. The aim of the present study was to evaluate the antioxidant and anti-inflammatory properties of the oil extracted from seeds of a N. sativa cultivar produced in the Marche region of Italy, and to determine if the thymoquinone content, antioxidant properties, and biological activity would decay during storage. Cytotoxicity and anti-inflammatory properties of N. sativa oil were tested in an in vitro model of low-grade inflammation in Simpson–Golabi–Behmel syndrome human pre-adipocytes. The fresh extracted oil (FEO) contained 33% more thymoquinone than stored extracted oil (SEO), demonstrating that storage affects its overall quality. In addition, the thymoquinone content in the N. sativa oil from the Marche region cultivar was higher compared with other N. sativa oils produced in the Middle East and in other Mediterranean regions. Pro-inflammatory cytokines (e.g., Interleukin (IL)-1alpha, IL-1beta, IL-6) were differently modulated by fresh and stored extracts from N. sativa oils: FEO, containing more thymoquinone reduced IL-6 levels significantly, while SEO inhibited IL-1beta and had a higher antioxidant activity. Total antioxidant activity, reported as µM of Trolox, was 11.273 ± 0.935 and 6.103 ± 0.446 for SEO and FEO (p = 1.255 × 10−7), respectively, while mean values of 9.895 ± 0.817 (SEO) and 4.727 ± 0.324 (FEO) were obtained with the 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay (p = 2.891 × 10−14). In conclusion, the oil capacity to counteract proinflammatory cytokine production does not strictly depend on the thymoquinone content, but also on other antioxidant components of the oil.

Implications of modified food choices and food-related lifestyles following the economic crisis in the Marche Region of Italy.

The economic crisis in Italy has led to profound changes in resource management not only at the macro level but also for individual families, causing substantial changes in different habits of Italians. The purpose of this research was to conduct a study on changes in family eating habits potentially triggered by the economic crisis was conducted in an area of the Marche Region in central Italy. The research was conducted in the period 2016 - 2017 by administering a specific and anonymous questionnaire. The interviewed people has reduced its food consumption. In particular, analyzing the results for the animal protein food group, there has been a reduction in purchase of beef, and an increase in that of pork. Overall fish consumption has decreased by 44%, with a decrease in the purchase of fresh fish, and an increase in that of canned fish products. Finally, consumers have reduced their purchases of fresh and canned legumes, fresh vegetables, and fresh fruit. The economic crisis seems to have changed the eating habits and food-related lifestyle choices of the subjects studied, especially in the urban area affected by the deeper economic depression. These changes are likely to have permanent social consequences, and deserve to be analyzed also in smaller territories in order to better understand the dynamics of individual choices and the social framework.