ISSR-PCR Method Research Articles

The use of the ISSR-PCR method for identifying domesticated animal breeds and species, inferring their population stuctures, and assessing gene pool similarity

The use of multilocus intermicrosatellite assay (ISSR-PCR) in combination with the method of k-clustering of population structures (Structure v2.2. program) has become a basis for a battery of tests of molecular genetic examination and for studying the gene pool of domesticated animal species. Possibilities are reviewed for analysis of the population structures of domesticated species at different levels (interspecific, interbreed, and intrabreed levels), for assessment of similarity of gene pools of breeds (intrabreed groups), as well as for identification of breeds and estimation of their consolidation, purity, and genealogical relationships.

ISSR (Inter Simple Sequence Repeats) as molecular markers to study genetic diversity in tarantulas (Araneae, Mygalomorphae)

Although all species of the Brachypelma genus are protected under CITES, few studies have been performed on the genetic structure of the populations of these endangered tarantulas. Here we propose, for the first time in spiders, to use ISSR (Inter Simple Sequence Repeat) technique to study the genetic variability of Mexican populations of Brachypelma vagans (Ausserer 1875). We used a nonlethal technique to collect samples from six populations in the Yucatan peninsula and we tested seven ISSR primers. Four of these primers gave fragments (bands) that were sufficiently clear and reproducible to construct a binary matrix and determine genetic variability parameters. We revealed a very high percentage of polymorphism (P 5 98.7%) the highest yet reported for tarantula spiders. Our results show that the ISSR-PCR method is promising for intraspecific variation of tarantula spiders.

Assessment of genetic diversity of tea (Camellia sinensis (L.) O. Kuntze) by inter-simple sequence repeat polymerase chain reaction

Twenty-five diverse tea (Camellia sinensis(L.) O. Kuntze) cultivars were analyzed using the simple sequence repeat anchored polymorearse chain reaction (SSR-anchored PCR) or Inter SSR-PCR (ISSR). Out of the 45 primers 12were chosen for final study. These amplified a total of 130 bands out of which108 (84%) were polymorphic. A dendrogram was constructed using UPGMA method revealed three distinct clusters of Cambod, Assamand China type, which concur with the known taxonomical classification of tea. These results suggest that the ISSR-PCR method is potentially useful for genetic fingerprinting and molecular taxonomic classification of tea genotypes.