In this study; hulled barley (Aydan Hanim and Tosun Pasa), hull-less barley cultivar (Yalin) and lines (INBYT6, INBYT16, INBYT18, ABA7, ABA8) were compared in grain yield and some quality characteristics, and the adaptability of hulled and hull-less barley lines to Isparta ecological conditions was determined during 2019-2021 years. The differences between genotypes and years in the examined characteristics were found to be statistically significant. According to the two-year average results, the plant height of the hull-less barley lines was shorter than the cultivars, the number of spikelets and grains and the thousand-seed weight were lower, the grain and biomass yields were lower except for the ABA8 and INBYT18 lines, and the test weight was higher in the others except the ABA7 line. As a result, it has been determined that hull-less barley can be grown in Isparta ecological conditions and INBYT18 (3702.7 kg ha-1) and ABA8 (3881.3 kg ha-1) lines are more suitable for these ecological conditions in terms of grain yield. In addition, it was determined that the crude protein rate was higher in hull-less genotypes.