Isozyme Analysis Research Articles

Patents on Endophytic Fungi.

Endophytic fungi are taxonomically and ecologically heterogeneous group of organisms, mainly belonging to the Ascomycotina and Deuteromycotina. Endophytes usually produce the enzymes necessary for the colonization of plant tissues. Endophytes are able to utilize components of plant cells without disturbing host metabolism, which is confirmed by isozyme analysis and studies on substrate utilization. The patents related to enzymes and metabolites produced by endophytic fungi are associated with their ecological significance. Application of metabolites and growth promoting factors produced from endophytic fungi, in the pharmaceutical and agricultural industries, is now well established. The patents on secretion of extracellular enzymes in vitro by endophytic fungi needed for cell wall degradation, support the hypothesis that fungal endophytes represent a group of organisms specialized to live within plant tissue. This review presents the patents granted on different aspects of endophytic fungi for the last 11 years. This expresses the scenario and impact of these patents regarding significance in human society. In the last few years, research and inventions regarding the different aspects of endophytic fungi beneficial for host plant as well as for human beings have been carried out, which is supported by the increasing number of patents granted on endophytic fungi.

Induction of apomixis and fixation of heterosis in Egyptian rice Hybrid1 line using colchicine mutagenesis

It is known that hybrid rice yields 15–20% over inbred varieties in first generation because of heterosis. However, heterosis is normally broken due to segregation. Applying apomixis produces plants as a clone of mother plant and overcomes the problem of breaking heterosis. In order to fix heterosis in the Egyptian rice Hybrid1, their seeds were mutagenized in 0.2% colchicine for two time periods 24 and 50h. After colchicine mutagenesis, rice seedlings were grown in the field till maturation and the resulted M1 seeds were sown in season 2 and plants were selected based on yield and homogeneity. Then, seeds were sown to be evaluated in season 3. Pollen fertility test, esterase isozyme analysis, and flow cytometry seed screening were performed to confirm the results of field selection of populations identical to control. Pollen fertility examination was performed on the populations of the third season. Pollens of populations 304, 298, 292, 284, 281, 154 and 149 were found to be completely sterile. However, these plants had high seed set percentage. The flow cytometry screening of the six yield-based identical populations and the control seeds showed that populations 220, 339, 351 and 298 have higher nuclear DNA content (C2) than untreated hybrid (C2 & C3). Results of flow cytometry clearly showed that population 298 has one peak (C2) and its endosperm was formed autonomously without fertilization. Although its pollen grains were sterile, it showed high seed set percentage. This indicates that heterosis was completely fixed by apomixis in this population.

КОНТРОЛЬ ГЕНЕТИЧЕСКОЙ ЧИСТОТЫ ЛИНИЙ И ГИБРИДОВ ПОДСОЛНЕЧНИКА МЕТОДОМ ИЗОФЕРМЕНТНОГО АНАЛИЗА

КОНТРОЛЬ ГЕНЕТИЧЕСКОЙ ЧИСТОТЫ ЛИНИЙ И ГИБРИДОВ ПОДСОЛНЕЧНИКА МЕТОДОМ ИЗОФЕРМЕНТНОГО АНАЛИЗА

Time Course Studies on Impact of Low Temperature Exposure on the Levels of Protein and Enzymes in Fifth Instar Larvae of Eri Silkworm, Philosamia ricini (Lepidoptera: satuniidae)

Lactate dehydrogenase (LDH; EC 1.1.1.27) and malate dehydrogenase (MDH; EC 1.1.1.37) are the enzymes involved in energy metabolism of Eri silkworm, Philosamia ricini. However, no previous study has been reported about effect of low temperature exposure on their levels in different tissues of Eri silkworm. The present study was aimed to the time-course effects of low temperature (~10°C) exposure of 5th instar P. ricini on the levels of protein and energy metabolism enzymes of three major tissues (haemolymph, silk gland and fat body). The Eri silkworm larvae, reared on fresh leaves of castor-oil-plant (Ricinus communis), were divided into 4 groups: a control group reared at 25 ± 2°C along with three experimental groups reared at 10 ± 1°C containing 50 larvae in each, for varying durations (2, 4 and 7 days). The cell free extract was prepared by centrifuging the tissue homogenate at 9000 g and used for biochemical estimations (total protein content, lactate dehydrogenase and malate dehydrogenase activities). For isozyme analysis, another set of homogenates (20% w/v) was prepared in buffer (0.2 M Tris HCl, pH 7.0) containing 0.2 M sucrose and 10 mM EDTA, and analyzed by native-PAGE followed by activity staining. The activities of lactate dehydrogenase and malate dehydrogenase showed significant decrease in haemolymph, whereas in fat bodies both enzymes showed increased activity. In silk gland, Lactate dehydrogenase activity decreased uniformly, whereas malate dehydrogenase activity increased at all exposure durations. The isozyme analysis revealed significant perturbations in their expression profiles. The low temperature exposure resulted into accumulation of protein content in haemolymph and depletion in silk gland and fat body tissues. Fat bodies emerged as the main energy producing organ under this condition. Lactate dehydrogenase and malate dehydrogenase displayed presence of only one isozyme in all the tissues tested. The isozyme behaviour of lactate dehydrogenase and malate dehydrogenase towards low temperature varied in different tissues. These results suggested that alterations in expression and functions of these enzymes might be associated to the acclimation of larvae at low temperature.

Diversity of Root-knot Nematodes Associated with Tubers of Yam (Dioscorea spp.) Established Using Isozyme Analysis and Mitochondrial DNA-based Identification.

The root-knot nematodes (RKN), Meloidogyne spp., represent an important threat to yam (Dioscorea spp.) production in West Africa. With the aim to establish the diversity of RKN species affecting yam tubers, for control and resistance screening purposes, surveys were conducted in the main yam producing areas of Nigeria. Galled tubers (N = 48) were collected from farmers' stores and markets in nine states in Nigeria and in one district in Ghana. RKN isolated from yam tubers were identified using enzyme phenotyping (esterase and malate dehydrogenase) and mitochondrial DNA (mtDNA) NADH dehydrogenase subunit 5 (Nad5) barcoding. Examination of 48 populations revealed that yam tubers were infested by Meloidogyne incognita (69%), followed by M. javanica (13%), M. enterolobii (2%), and M. arenaria (2%). Most of the tubers sampled (86%) were infected by a single species, and multiple species of RKN were detected in 14% of the samples. Results of both identification methods revealed the same species, confirming their accuracy for the identification of these tropical RKN species. In addition to M. incognita, M. javanica, and M. enterolobii, we report for the first time M. arenaria infecting yam tubers in Nigeria. This finding extends the list of yam pests and calls for caution when developing practices for yam pest management.

Molecular Analysis of Date Palm Genetic Diversity Using Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeats (ISSRs).

The date palm is an ancient domesticated plant with great diversity and has been cultivated in the Middle East and North Africa for at last 5000years. Date palm cultivars are classified based on the fruit moisture content, as dry, semidry, and soft dates. There are a number of biochemical and molecular techniques available for characterization of the date palm variation. This chapter focuses on the DNA-based markers random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) techniques, in addition to biochemical markers based on isozyme analysis. These techniques coupled with appropriate statistical tools proved useful for determining phylogenetic relationships among date palm cultivars and provide information resources for date palm gene banks.

Proteomic analysis of isozymes in Butea superba leaves

The proliferation of protein abundances under influence of seasonal changes are involved in cellular plant metabolic pathways. The protein profiles in relevant with seasonal variations in Butea superba leaves collected in winter, summer and rainy season were evaluated by two-dimensional polyacrylamide gel electrophoresis coupled with a nanoflow liquid chromatography coupled to electrospray ionization quadrupoletime- of-flight tandem mass spectrometry. The 84 proliferated protein spots were sum up of total proteins, which were found in 3 season-collected plant leaves and were classified into 11 functional groups. Eight proteins which exhibited the alteration of abundant levels in different seasons were found involved in carbohydrate and energy metabolism, photosynthesis, secondary metabolism, stress, RNA metabolism, ROS scavenging and detoxifying, and protein destination and storage. There were 2 proteins exhibited obviously isozyme polypeptide sequences. The variable physiological status within the plant leaf might be influenced by the quantitative and qualitative proliferated protein presences with response to seasonal changes and thus allowing plant to survive during severe abiotic stresses during seasonal changes.

KERAGAMAN GENETIK DAN PERMUDAAN ALAM PUSPA (Schima wallichii (DC.) Korth.) DI TAMAN NASIONAL GUNUNG MERAPI PASCAERUPSI TAHUN 2010

Schima wallichii (DC.) Korth. (puspa) is a native vegetation of Gunung Merapi National Park (GMNP). This species has an important function for soil and water conservation in GMNP area, especially in the area with high slope. Puspa is also one of prioritised species planted in the forest ecosystem restoration project of GMNP after 2010 volcano eruption. Information on genetic diversity and natural regeneration potential of each puspa stand groups is then very essential for the success of forest ecosystem restoration in GMNP. Genetic diversity parameters of puspa is assessed by isozyme analysis, while its natural regeneration is evaluated by vegetation analysis. Selection of samples on stand groups of puspa for genetic diversity and natural regeneration analysis is based on levels of vegetation damage (low and moderate) and the planting plots of ecosystem restoration area. The results showed that the stands with low damage level located at Gunung Malang and Mriyan have higher genetic diversity than those with moderate damage level (Balerante and Tlogolele). Stand with the highest genetic diversity is Gunung Malang, with the mean observed heterozigosities (Ho) of 0.284 and minimum fixation index value (FIS) of 0.0508. Stand in the Ecosystem Restoration Demonstration Plot has the lowest level of genetic diversity (Ho = 0.1936; FIS = 0.1127). Regarding natural regeneration potential, the stand group of Gunung Malang is the highest.

Study on genetic diversity in chilli (Capsicum annuum) based on multivariate analysis and isozyme analysis

Thirty seven diverse chilli (Capsicum annuum) genotypes were studied for 22 growth, yield and fruit quality traits. Multivariate analysis grouped the genotypes into 7 clusters. Cluster IV was largest containing 14 genotypes. Inter cluster distance was maximum between cluster V and VII (248.09), and minimum between cluster I and II (57.80). Cluster VII was most heterogeneous (intra-cluster divergence value 191.25) and Cluster II was most homogeneous (30.25). Genotypes were also analyzed for peroxidase enzyme polymorphism using gel electrophoresis which resulted seven electrophoretic bands (Rf 0.19 to 0.59) and grouped the genotypes into 6 zymotypes. Zymotype P4 included maximum (13) number of genotypes. Number of clusters in peroxidase and multivariate analysis were almost same but distribution of genotypes varied. 73% of total genotypes showed similar pattern of grouping suggesting that the two methods are complementary to each other and should be carried out simultaneously to determine genetic diversity more effectively. Considering variability and diversity analysis of the genotypes, CUCH-4 from Cluster-II (& Zymotype-P2) and CUCH-31, CUCH-34 and CUCH-35 from Cluster-VII (& Zymotype-P4) were identified as promising genotypes which can be used in further crop improvement programme.

Exploiting cucurbitaceous species as rootstocks for management of Fusarium wilt (Fusarium oxysporum) in bitter gourd

Bitter gourd is affected by various kinds of pathogens, of which Fusarium wilt Fusarium oxysporum plays a vital role. The present investigation focuses on the role of defense related enzymes which impart resistance against Fusarium wilt. A total of ten cucurbitaceous rootstocks and two bitter gourd scions were screened against Fusarium wilt pathogen under in vitro. Results on screening against Fusarium wilt revealed that Citrullus colocynthis, Cucumis metuliferus and Cucurbita moschata exhibited no symptom and manifested as resistant to Fusarium wilt and the least percent incidence of 21.62, 37.44 and 48.90 was observed in Luffa cylindrica followed by Momordica charantia var. muricata rootstock (23.58, 42.18 and 50.34) at 30, 45 and 60 days after inoculation. Seedlings of aforementioned species were harvested at 0, 7, 14, 21, 28 and 35 days after challenge inoculation and assayed for defense related enzymes activity. Significant increases in the activities of peroxidase (PO), polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL) activity was observed in resistant rootstocks viz., C. colocynthis, C. metuliferus and C.moschata followed by moderately resistant rootstocks viz., M. charantia var. muricata and L. cylindrica. Native page analysis of both PO and PPO isozymes was carried out for the time course and examined. Isozyme analysis revealed that unique PO and PPO isozymes were induced in aforementioned resistant rootstocks. From this study point out that the cucurbitaceous species viz., kumatikai (C. colocynthis), African horned cucumber (C. metuliferus) and pumpkin (C. moschata) with high or moderate levels of these biochemical constituents suffered less for Fusarium wilt pathogen and these rootstocks served as the best rootstocks for grafting with bitter gourd scions followed by mithipakal(M. charantia var. muricata) and sponge gourd (L. cylindrica).

Meloidogyne species infesting tomatoes, cucumbers and eggplants grown in Kahramanmaraş Province, Turkey

In 2009 and 2010, 107 samples of roots and associated soil were collected from vegetable-growing fields in Andirin, Narli, Pazarcik and Turkoglu of Kahramanmaras Province, Turkey. Populations of root-knot nematode were found in tomatoes, eggplants and cucumbers and the species identified using isozymes analysis, mainly esterase resolved by PAGE (Polyacrylamide Gel Electrophoresis) and perineal patterns of nematode females. A single species of root-knot nematode, Meloidogyne incognita was identified from the samples collected. Two esterase phenotypes, one with a single band (I1) and the other with double bands (I2) were found at proportions of 8.5 and 15.8% of total samples, respectively. About 24% of the fields were found to be infested

Enzymatic and thermodynamic profiles of a heterotetramer lactate dehydrogenase isozyme in swine

Lactate dehydrogenase (LDH) is a glycolytic enzyme that catalyzes the final step of glycolysis and produces NAD+. In somatic cells, LDH forms homotetramers and heterotetramers that are encoded by two different genes: LDHA (skeletal muscle type, M) and LDHB (heart type, H). Analysis of LDH isozymes is important for understanding the physiological role of homotetramers and heterotetramers and for optimizing inhibition of their enzymatic activity as it may result in distinct effects. Previously, we reported that hydroxychloroquine (HCQ) inhibited LDH activity, but we did not examine isozyme specificity. In the present study, we isolated heterotetrameric LDH (H2M2) from swine brain, determined its kinetic and thermodynamic properties, and examined the effect of HCQ on its activity compared to homotetrameric LDH isozymes. We show that: (1) the Km values for H2M2–mediated catalysis of pyruvate or lactate were intermediate compared to those for the homotetrameric isozymes, M4 and H4 whereas the Vmax values were similar; (2) the Km and Vmax values for H2M2–mediated catalysis of NADH were not significantly different among LDH isozymes; (3) the values for activation energy and van't Hoff enthalpy changes for pyruvate reduction of H2M2 were intermediate compared to those for the homotetrameric isozymes; (4) the temperature for half residual activity of H2M2 was closer to that for M4 than for H4. We also show that HCQ had different affinities for various LDH isozymes.

Genetic diversity and reproductive traits in triploid and tetraploid populations of Gladiolus tenuis (Iridaceae)

Most perennial herbaceous plants are able to reproduce vegetatively as well as sexually. Sometimes, such plants may lose the capacity for sexual reproduction. We have studied the case of sterility in triploid populations (2n = 3x = 45) of Gladiolus tenuis M.Bieb. in a considerable part of its area of distribution. Initially, we recorded the presence of a large clone of G. tenuis to the east of the Volga River, as a result of isozyme analysis. We also used AFLP fingerprinting to genotype 55 samples from 10 populations of G. tenuis and one population of the closely related G. imbricutus L. This analysis revealed an extremely low genetic diversity in sterile triploid populations of G. tenuis and a rather high genetic diversity in fertile tetraploid populations (2n = 4x = 60) over most of the area of this species. Genetic distances between fertile and sterile populations of G. tenuis were similar to those between different species of gladioli. It appears that a single sterile genotype has spread vegetatively over 800 km, propagating by daughter corms. The study of the reproductive features of G. tenuis suggests that the cause of sterility may be self-incompatibility between individuals of the clone.

Keragaman Genetik Beberapa Genotipe Jarak Pagar (Jatropha curcas L.) Berdaya Hasil Tinggi Berdasarkan Karakter Morfologi, Agronomi, dan Isozim

<p>The interest in using Jatropha curcas L. from the<br />family Euphorbiaceae for the production of biofuel is rapidly<br />growing. The research objective was to determine genetic<br />variation of several high yielding physic nuts based on their<br />morphology, agronomy, and isozyme characters. The<br />research used Completely Randomized Design with three<br />replications. The treatment was consisted of 8 genotypes i.e.<br />IP-1A, IP-1M, IP-2P, Lombok Timur, Lombok Tengah,<br />Lombok Barat, Sumbawa, and Bima. Analysis of isozyme of<br />the eight genotypes was also conducted according to 5<br />enzyme systems, i.e. peroksidase, esterase, aspartat<br />aminotransferase, malat dehidrogenase, and alcohol<br />dehidrogenase. Observation was done on qualitative and<br />quantitative characters as well as banding pattern derivedisozymes.<br />The results showed that genetic variation was low<br />when based on qualitative characters and isozyme (0-25%)<br />but relatively high when based on selected quantitative<br />characters analysis (17-81%). Analysis of combined<br />qualitative, quantitative, and isozyme characters still gave<br />low genetic variation (6-33%). Based on the quantitative<br />characters at similarity coefficient of 46% the genotypes can<br />be devided into three clusters. Improved population<br />genotypes, i.e. IP-1A, IP-1M, and IP-2P were placed in 3<br />different clusters, while other genotypes from NTB area<br />were grouped in the same cluster. Therefore, selection<br />among population of the same ecotype based on agronomic<br />characters such as yield components, yield and oil content<br />was suitable in Jatropha improvement, especially when<br />genetic variation was low. Furthermore, introduction,<br />mutation and crossing are suggested to increase genetic<br />variation of current Jatropha collection.</p>

Morphological, Biometrical, Biochemical, and Molecular Characterization of the Coffee Root-Knot Nematode Meloidogyne megadora

Meloidogyne megadora infects coffee trees, an economically important crop worldwide. The accurate identification of M. megadora is essential for the development of preventive measures to avoid the dispersion of this pathogen and establishment of efficient and sustainable integrated pest management programs. One M. megadora isolate was studied by biometrical, biochemical, and molecular characteristics (random amplified polymorphic DNA [RAPD] and PCR of internal transcribed spacer [ITS] region). Biometrical characteristics of M. megadora females, males, and second-stage juveniles were similar to the original description. Biochemical studies revealed a unique enzyme pattern for M. megadora esterases (Me3) that allowed for species differentiation. Three RAPD primers (OPG-4, OPG-5, and OPG-6) produced specific bands to all Meloidogyne spp. studied: M. megadora, M. arenaria, M. incognita, and M. javanica. Molecular analysis of the ITS region resulted in an amplification product of 700 bp. The phylogenetic relationship between M. megadora and several Meloidogyne spp. sequences was analyzed, revealing that M. megadora clearly differs from the most common root-knot nematode species. Based on the studies conducted, isozyme analysis remains a useful and efficient methodology for M. megadora identification when females are available. Further studies will be needed to convert the M. megadora differential DNA fragment obtained by RAPD and develop a species-specific sequence-characterized amplified region PCR assay for its diagnosis based on second-stage juveniles.

C.chacoense, C. pubescens ve BU İKİ TÜRÜN FARKLI FORMLARININ İZOENZİM MARKÖRLERİ İLE TANILANMASI

Isozymes have been used for a long time to reveal the genetic differences between populations, to solve the problems related to taxonomy, and to identify cultivars. This study was conducted to reveal the possibilities of using isozyme polymorphism to show intra-and interspecific variation between different Capsicum species. Experiment results showed that while isozyme analysis is rather not strong enough to reveal the intraspecific variation, it can be used to reveal the interspecific variation.

Diversity evaluation based on morphological, physiological and isozyme variation in genetic resources of garlic (Allium sativum L.) collected worldwide.

The aim of this study was to obtain primary information about the global diversity of garlic (Allium sativum L.) by evaluating morphological, physiological and isozyme variation. A total of 107 garlic accessions collected worldwide were grown in Yamaguchi, Japan. Five morphological traits (bulb weight, bulb diameter, number of cloves per bulb, number of bulbils and scape length) and one physiological trait (bolting period) of the collected garlic showed wide variation. Meanwhile, a total of 140 garlic accessions, including the 107 mentioned above, were characterized by leucine aminopeptidase (LAP) and phosphoglucoisomerase (PGI) isozyme analyses; they clearly showed polymorphisms in putative isozyme loci (Lap-1, Lap-2 and Pgi-1). Allelic frequencies were estimated in each group of accessions categorized by their geographical origin, and the observed (Ho) and expected (He) heterozygosities were calculated. The allelic frequencies differed between groups. A principal component analysis based on morpho-physiological data indicated a grouping of the garlic accessions into Central Asian and Northern Mediterranean groups as well as others. We discuss the roles of artificial and natural selection that may have caused differentiation in these traits, on the assumption that ancestral domesticated garlic populations have adapted in various regions using standing variation or mutations that accumulated during expansion, and have evolved along with human-preferred traits over a long history of cultivation.

Morphology and isozyme band-profile as sexual determinant of nutmeg plant (Myristica fragrants Houttj).

Sex information in nutmeg plant is important to distinguish between male and female species. The recommendation of optimum sex-ratio for a nutmeg plantation is 1:10. This research is aimed to discover the morphological characteristic of nutmeg plant and the difference of leaf pattern as sexual determinant between male and female plant with the comparison of isozyme analysis. The result of morphological identification provided from 3 research farm location indicated that female nutmeg was higher, with longer and wider leaf, more vein, bigger diameter of stem and branching angle as compared to male nutmeg. Two parameters which were widht of leaf and branching angle showed significant differences, where female nutmeg plants have wider leaves and branching angle. Isozyme analysis suggested that the analysis of peroxidase enzyme (PER) was better and able to provide more information than aspartarte amino transferase (AAT) and acid phospatase (ACP) Enzymes.

Screening of Staple Food Crops for their Phytosterol and Nutritional Contents to Determine their Hyperlipidemia Controlling Potential

Screening of Staple Food Crops for their Phytosterol and Nutritional Contents to Determine their Hyperlipidemia Controlling Potential Phytosterol rich food uptake is a key factor in controlling hyperlipidemia. This goal can be achieved by screening staple foods for their phytosterol contents along other nutritional facts. Therefore, seven staple food crops (i.e. wheat, kabuli chickpea, desi chickpea, barley, rice, corn and millet) were evaluated for their dietary benefits, with a special focus on phytosterol contents. Plants were tested for their nutritional and palatability components through organic extraction and calorimetric methods. Moreover, isozyme analysis of Oxidosqualene Cyclase was performed through Native Polyacrylamide Gel Electrophoresis. Data were analysed through MYSTAT (Kroeger, Chicago, USA) and GELANALYZER (Lazar, Hungry). It was recorded that barley (Malt) had maximum phytosterol contents (0.239 g/ kg), which can be potentially used for control of hyperlipidemia. Corn was the most palatable and safest food crop due to its biochemical combination related to palatability. Vitamin analysis revealed that barley was rich in all tested water soluble vitamin contents except Folic acid; which was the highest in corn (0.006 g/ kg). Moreover, barley exhibited maximum number of isozymes for oxidosqualene cyclase (OSC). Other crops had variable number of isozymes for OSC, but lesser than barley. Current investigation is highly important in relation to population health management. It recommends barley as a recommended staple food crop for control of hyperlipidemia.

Isozyme analysis based genetic fidelity assessment of micropropagated banana plants

Isozyme studies of micropropagated and mother plants of banana cvs. Matti, Ney Poovan, Kechulepa, Dwarf Cavendish, Malbhog, Champa, B.B. Battisa and FHIA-1 were done to test their genetic fidelity. The banding patterns as revealed by electrophoretic variations were evaluated with respect to isozymes of acid phosphatase, catalase, esterase and peroxidase as markers. The genetic fidelity of micropropagated plants and the relationship of the different cultivars were determined by dendrogram using numerical taxonomy and multivariate analysis system(NTSYS). A clustered dendrogram was prepared by unweighted pair group method using averages (UPGAMA) method. At 87% similarity, the micropropagated and mother plants were clustered in four groups reflecting their genomic constitution. Cvs. Matti (AA) and Dwarf Cavendish (AAA) with similar ‘A’ genome were categorized in Cluster I. Cluster II comprised of cvs. Ney Poovan (AB), B.B. Battisa (ABB) and FHIA-1(AAAB) with genomic constitution of both ‘A’ and ‘B’ type. Cvs. Champa (AAB) and Malbhog (AAB) with similar genome were grouped inCluster III. Cluster IV contained the cv. Kechulepa (BB) having only ‘B’ genome. However, there was no somaclonal variation among the micropropagated plants and they showed 100% genetic similarity. Thus, the isozyme studies could be a reliable marker for testing the genetic fidelity of micropropagated plants and for evaluating the diversity among the banana germplasm.