Transversely isotropic rocks, which are special anisotropic materials, are widely encountered in civil, mining, petroleum, geothermal, and radioactive waste-disposal engineering. Rock is frequently subject to cyclic loads resulting from natural and human-caused events. However, to date, the fracture mechanism of transversely isotropic rocks under cyclic loading remains poorly understood. To address this gap, uniaxial monotonic-loading and cyclic-loading tests were performed on slate specimens by the MTS815 system, during which acoustic emission (AE) signals inside the rock were monitored, and finally the fracture surfaces of the tested rock were scanned by scanning electron microscopy (SEM). Through these tests, the anisotropic mechanical properties, damage evolution, AE characteristics and fracture pattern of slate as a transversely isotropic rock were studied. The results show that the peak strength of specimens varies with the loading–foliation angle under monotonic and cyclic loading, following a U-shaped trend. The deformation modulus during unloading is more capable of characterizing the damage inside the specimen than that during loading. By defining the damage degree based on dissipation energy, it is found that the damage variable is influenced by the loading–foliation angle and the cyclic stress step. The AE characteristics of specimens exhibit significant anisotropy, closely correlated to the loading condition and loading–foliation angle. Regardless of cyclic stress step, the AE counts of specimens with a loading–foliation angle of 0° are mainly distributed near the peak region, whereas those of specimens with other loading–foliation angles occur primarily in the early stage of each cyclic loading. Finally, it is revealed that the fracture mechanism of slate specimens is determined by the loading–foliation angle, loading condition, and cyclic stress step.