Glyphosate Isopropylamine Salt Research Articles

Postemergence Control of Hybrid Bermudagrass (Cynodon transvaalensisBurtt-Davy ×Cynodon dactylon)

Bermudagrass [Cynodon dactylon(L.) Pers.] control for at least 1 yr is necessary to establish other forage species in pasture and hay field renovations, or to plant pine (Pinusspp.) in infested fields. Potential herbicides for hybrid Bermudagrass control were evaluated using single, repeat, and multiyear applications. Repeat applications were made 30 d after the first application beginning in mid-August each year. Imazapyr applied once at 0.56 or 1.12 kg/ha controlled hybrid Bermudagrass (cv. ‘Tifton 44’) 88 and 97%, respectively, 52 wk after treatment with no difference between rates observed. Additionally, imazapyr applied for two consecutive years controlled hybrid Bermudagrass 100%. Glyphosate isopropylamine salt and glyphosate trimethylsulfonium salt, applied for 1 yr at rates of 4.2 or 1.7 fb (followed by) 1.7 kg ae/ha and 4.8 or 1.9 fb 1.9 kg ae/ha, respectively, provided between 70 and 78% control at 52 wk after the last treatment (WALT). Hybrid Bermudagrass control from either formulation of glyphosate applied for two consecutive years ranged between 79 and 91% at 52 WALT. Relative to a 1-yr application program, either glyphosate formulation applied for two consecutive years did not significantly improve Bermudagrass control at 52 WALT. The addition of fluazifop-P at 0.42 kg ai/ha or clethodim at 0.2 kg ai/ha to glyphosate formulations did not significantly improve hybrid Bermudagrass control relative to glyphosate applied alone. However, a tank-mix of clethodim plus either formulation of glyphosate applied for two consecutive years generally improved hybrid Bermudagrass control relative to applications in only 1 yr.

Cytogenetic effect of technical glyphosate on cultivated bovine peripheral lymphocytes

A technical herbicide containing isopropyl amine salt of glyphosate was tested for induction of chromosome aberrations (CA) and sister chromatid exchanges (SCE) in cultured bovine peripheral lymphocytes. Cultures were exposed to a glyphosate formulation at concentrations ranging from 28 to 1120 μmol/l without and with metabolic activation. No clastogenic effect of the herbicide was found. Its genotoxic effect was confirmed in the SCE assay after 24 h of incubation. A statistically significant elevation in SCE induction was observed in each of the donors after application of the product at doses ranging from 56 to 1120 μmol/l. The highest concentrations (560 and 1120 μmol/l) also caused reduction of mitotic and proliferation indices. In the 2 h-assay with metabolic activation a statistically significant frequency of SCE was observed only in cultures treated with the agent at a concentration of 140 μmol/l.

Optimizing herbicidal efficacy of glyphosate isopropylamine salt through ammonium sulphate as surfactant in oil palm ( Elaeis guineensis) plantation in a rainforest area of Nigeria

A randomized complete block field trial was conducted over 2 years to evaluate the herbicidal efficacy of glyphosate isopropylamine salt (Roundup ®) applied singly at 1.41 kg a.e. ha −1 or in combination with ammonium sulphate (AMS) as surfactant at 0.5, 1.0, 1.5, and 2.0% (wt/v) in a 5-year old oil palm plantation of the Federal University of Technology, Akure located in the rainforest vegetation zone of Nigeria. Assessment of herbicidal efficacy based on the Henderson-Tilton formula indicated that glyphosate applied singly or in combination with varying concentrations of AMS proved highly effective in controlling most of the grasses and broadleaved weeds prevalent in the experimental plots. Herbicidal efficacy on total weed density, weed fresh weight as well as on weed dry weight was found to increase in the order of increasing concentration of AMS. Regressing percentage herbicidal efficacy ( Y) against increasing concentration levels of AMS ( X) indicated significant ( P ⩽ 0.001 ) positive relationships with an average correlation coefficient ( r) of +0.95 in both years. These results confirm the potential of AMS as an effective surfactant that can be exploited in glyphosate-based weed management schemes in the sub-humid tropical environment.

Glyphosate-Resistant Horseweed (Conyza canadensis) in Mississippi

Survival of horseweed in several glyphosate-tolerant cotton and soybean fields treated with glyphosate at recommended rates preplant and postemergence was observed in Mississippi and Tennessee in 2001 and 2002. Plants originating from seed collected from fields where horseweed escapes occurred in 2002 were grown in the greenhouse to the 5-leaf, 13- to 15-leaf, and 25- to 30-leaf growth stages and treated with the isopropylamine salt of glyphosate at 0, 0.025, 0.05, 0.1, 0.21, 0.42, 0.84, 1.68, 3.36, 6.72, and 13.44 kg ae/ha to determine if resistance to glyphosate existed in any biotype. All biotypes exhibited an 8- to 12-fold level of resistance to glyphosate when compared with a susceptible biotype. One resistant biotype from Mississippi was two- to fourfold more resistant than other resistant biotypes. Growth stage had little effect on level of glyphosate resistance. The glyphosate rate required to reduce biomass of glyphosate-resistant horseweed by 50% (GR50) increased from 0.14 to 2.2 kg/ha as plant size increased from the 5-leaf to 25- to 30-leaf growth stage. The GR50rate for the susceptible biotype increased from 0.02 to 0.2 kg/ha glyphosate. These results demonstrate that the difficult-to-control biotypes were resistant to glyphosate, that resistant biotypes could survive glyphosate rates of up to 6.72 kg/ha, and that plant size affected both resistant and susceptible biotypes in a similar manner.

Control of Volunteer Herbicide-Resistant Wheat and Canola

Volunteer crops resistant to glyphosate and other herbicides pose a potential problem for Pacific Northwest (PNW) growers that rely on glyphosate for control of volunteer crops and weeds during fallow and before planting. Herbicides for control of volunteer herbicide-resistant wheat and canola in PNW conservation tillage systems were evaluated during 2000 and 2001 near Ralston, WA, and Moscow, ID. Paraquat + diuron controlled glyphosate- and imidazolinone-resistant wheat ≥90%, and glyphosate controlled imidazolinone-resistant wheat 88 to 96% 14 d after treatment (DAT). Glyphosate- and imidazolinone-resistant wheat were controlled only 58 to 85% with quizalofop-P and clethodim 14 DAT. By 21 DAT, imidazolinone-resistant wheat control with clethodim and quizalofop-P was ≥93%, but the longer time period required for control to reach an acceptable level could increase disease and insect problems associated with volunteer wheat. Volunteer glyphosate-resistant canola was controlled 92 and 97% 14 DAT and 76 and 98% 21 DAT with paraquat and paraquat + diuron, respectively. Treatments that contained glyphosate controlled imidazolinone- and glufosinate-resistant canola >84% 14 DAT. By 21 DAT, control of imidazolinone- and glufosinate-resistant canola was 94 to 98% with paraquat + diuron and all glyphosate treatments, except glyphosate–isopropylamine salt (IPA) + glufosinate (88 to 93%) and glyphosate-IPA + paraquat (67 to 85%). In these studies, paraquat + diuron was the best alternative to glyphosate for controlling volunteer herbicide-resistant wheat and canola.

Cotton and Weed Response to Glyphosate Applied with Sulfur-Containing Additives

Field studies were conducted to assess two sulfur-containing additives for use with glyphosate applied postemergence to glyphosate-resistant cotton for the control of sicklepod and yellow nutsedge. Neither diammonium sulfate (AMS) nor ammonium thiosulfate (ATS), both applied at 2.24 kg/ha, increased control of either species. Effective control of both species was dependent on glyphosate (isopropylamine salt) rate alone, with optimum control at 1.26 kg ae/ha. Plant-mapping data further indicated that sulfur-containing additives generally had no effect on either cotton fruiting patterns or yield. However, applying glyphosate at any rate did increase seed cotton yield in 2 of 3 yr vs. no glyphosate. In addition, applying glyphosate at any rate resulted in an increase in the number of bolls vs. no glyphosate in the following plant-mapping responses: total number of bolls per plant, number of abcised bolls per plant, bolls at the top five sympodial nodes, and bolls at positions 1 and 2 on the sympodia. Glyphosate absorption and subsequent translocation, as influenced by the addition of the sulfur-containing additives, was evaluated using radiotracer techniques. Glyphosate absorption after 48 h was 86, 63, and 37% of amount applied in cotton, sicklepod, and yellow nutsedge, respectively. Absorption by sicklepod and yellow nutsedge was not affected by the addition of either of the additives. Absorption by cotton was reduced by ATS but was not affected by AMS. In yellow nutsedge and cotton, glyphosate concentration in the treated area and adjacent tissue was not affected by either additive. A greater portion of glyphosate was translocated away from the treated area in sicklepod with glyphosate plus AMS (32%) than with glyphosate plus ATS (21%). AMS and ATS may be used in glyphosate-resistant cotton without the risk of either crop injury or yield reduction. However, their use for increased control of annual weed species, such as sicklepod and yellow nutsedge, may not be warranted.

Effect of Glyphosate Spray Coverage on Control of Pitted Morningglory (Ipomoea lacunosa)1

Greenhouse and field experiments were conducted to investigate the effect of glyphosate rate and degree of glyphosate spray coverage on pitted morningglory control. Pitted morningglory in the two-, four-, and six-leaf growth stages were treated with the isopropylamine salt of glyphosate at 0.28, 0.56, 0.84, 1.12, 1.40, and 1.68 kg ai/ha. Two- and four-leaf plants were controlled 98% with 1.68 kg/ha glyphosate, whereas six-leaf plants were controlled 68%. Control of two-, four-, and six-leaf plants with the commonly used field rate of 1.12 kg/ha was 68, 60, and 50%, respectively. In a separate greenhouse study, four-leaf pitted morningglory plants with 0, 33, 66, or 100% of their total leaf area exposed to herbicide spray were treated with 0.84, 1.68, or 3.36 kg/ha glyphosate. Increasing glyphosate rate from 0.84 to 3.36 kg/ha increased control from 36 to 88%. In contrast, increasing percent leaf exposure to glyphosate from 0 to 100% increased control from 57 to 75%. Increasing glyphosate rate from 0.84 to 1.68 kg/ha always improved control. However, increasing glyphosate rate from 1.68 to 3.36 kg/ha was beneficial only when no leaves were exposed to the spray solution. In the field, glyphosate spray coverage decreased from 85 to 40% as plant density increased from 1 to 32 plants/m2. However, control decreased only 11% (90 to 79%) between the highest and lowest levels of glyphosate spray coverage. These results demonstrated that inadequate control of pitted morningglory with glyphosate was more related to tolerance than glyphosate spray coverage. Glyphosate rates higher than 1.68 kg/ha may be beneficial when spray coverage is severely limited or when plants are beyond the four-leaf growth stage.Nomenclature: Glyphosate; pitted morningglory, Ipomoea lacunosa L. #3 IPOLA.Additional index words: Biomass reduction, herbicide efficacy, herbicide tolerance, plant density, plant population.Abbreviations: LAI, leaf area index; PAR, photosynthetically active radiation; WAT, weeks after treatment.

Glyphosate Poisoning

Glyphosate is used extensively as a non-selective herbicide by both professional applicators and consumers and its use is likely to increase further as it is one of the first herbicides against which crops have been genetically modified to increase their tolerance. Commercial glyphosate-based formulations most commonly range from concentrates containing 41% or more glyphosate to 1% glyphosate formulations marketed for domestic use. They generally consist of an aqueous mixture of the isopropylamine (IPA) salt of glyphosate, a surfactant, and various minor components including anti-foaming and colour agents, biocides and inorganic ions to produce pH adjustment. The mechanisms of toxicity of glyphosate formulations are complicated. Not only is glyphosate used as five different salts but commercial formulations of it contain surfactants, which vary in nature and concentration. As a result, human poisoning with this herbicide is not with the active ingredient alone but with complex and variable mixtures. Therefore, It is difficult to separate the toxicity of glyphosate from that of the formulation as a whole or to determine the contribution of surfactants to overall toxicity. Experimental studies suggest that the toxicity of the surfactant, polyoxyethyleneamine (POEA), is greater than the toxicity of glyphosate alone and commercial formulations alone. There is insufficient evidence to conclude that glyphosate preparations containing POEA are more toxic than those containing alternative surfactants. Although surfactants probably contribute to the acute toxicity of glyphosate formulations, the weight of evidence is against surfactants potentiating the toxicity of glyphosate. Accidental ingestion of glyphosate formulations is generally associated with only mild, transient, gastrointestinal features. Most reported cases have followed the deliberate ingestion of the concentrated formulation of Roundup (The use of trade names is for product identification purposes only and does not imply endorsement.) (41% glyphosate as the IPA salt and 15% POEA). There is a reasonable correlation between the amount ingested and the likelihood of serious systemic sequelae or death. Advancing age is also associated with a less favourable prognosis. Ingestion of >85 mL of the concentrated formulation is likely to cause significant toxicity in adults. Gastrointestinal corrosive effects, with mouth, throat and epigastric pain and dysphagia are common. Renal and hepatic impairment are also frequent and usually reflect reduced organ perfusion. Respiratory distress, impaired consciousness, pulmonary oedema, infiltration on chest x-ray, shock, arrythmias, renal failure requiring haemodialysis, metabolic acidosis and hyperkalaemia may supervene in severe cases. Bradycardia and ventricular arrhythmias are often present pre-terminally. Dermal exposure to ready-to-use glyphosate formulations can cause irritation and photo-contact dermatitis has been reported occasionally; these effects are probably due to the preservative Proxel (benzisothiazolin-3-one). Severe skin burns are very rare. Inhalation is a minor route of exposure but spray mist may cause oral or nasal discomfort, an unpleasant taste in the mouth, tingling and throat irritation. Eye exposure may lead to mild conjunctivitis, and superficial corneal injury is possible if irrigation is delayed or inadequate. Management is symptomatic and supportive, and skin decontamination with soap and water after removal of contaminated clothing should be undertaken in cases of dermal exposure.

The biology of Canadian weeds. 129. Phragmites australis (Cav.) Trin. ex Steud.

Phragmites australis (Cav.) Trin. ex Steud.-common reed, is a perennial, emergent aquatic plant with annual cane-like stems developed from an extensive rhizome system. It grows in low-lying wet areas such as fresh and salt-water marshes, drainage ditches, shallow lake edges, sandy banks, roadsides, woodlands and rocky places. Stems can reach up to 6.0 m in height, vary in diameter from 4 to10 mm and have 10 to 25 cm long hollow internodes. Clones are extended by perennial rhizomes with extensive aerenchymatous tissue that supplies oxygen. Roots develop from rhizomes and other submerged parts of shoots. Leaves are smooth, alternate with narrow-lanceolate laminae, 20 to 70 cm long and 1 to 5 cm broad, and tapering to long slender points. The inflorescence is a terminal panicle, often 30 cm long, dull purple to yellow, with main branches bearing many spikelets. Seed production and germination are extremely variable and comparatively rare in many populations. Phragmites australis carries out photosynthesis through the C3 pathway (or a variation thereof). Studies of genetic variation through isozyme and other molecular methods suggest that the populations are very closely related, and that variation in the metapopulation is small. Chloroplast DNA sequences of two non-coding regions indicate that non-native introduced genotypes of P. australis have displaced native genotypes in parts of North America. Phragmites australis often forms extensive monocultures in North America. As a consequence, habitat quality and species diversity have been documented to decline. However, in roadside populations it is effective in taking up many typical heavy metals that originate from nearby highways and buildings. Phragmites australis is found in all Canadian provinces and the Northwest Territories, but not in the Yukon Territory or Nunavut. The infestation of P. australis is most severe in the Great Lakes region and its migration is primarily mediated through rivers, canals and waterways but roadways are increasingly becoming important. Changes in the water regime have been linked to its success and could ultimately result in changes to the floristic composition of a habitat. Rodeo™, an aqueous solution of the isopropylamine salt of glyphosate, is most frequently used to control P. australis populations. Other methods of control include cutting, burning, and drainage of the species’ habitat. As P. australis is considered to be invasive in North America, introduction of biological control agents is now being investigated. Key words: Phragmites australis; common reed; Canadian distribution; wetlands; invasive weed

Acute eye irritation study of a mixture of glyphosate isopropylamine salt and 2,4 D-isopropylamine

This is an acute irritation study of the eye of a mixture of herbicides containing 240 g/l of glyphosate isopropylamine and 2,4 D-isopropylamine in the rabbits. This study was conducted according the methods as dercribed by the United States Environmental Protection Agency (EPA). Our results show that the combination of the herbicides causes mild conjunctivitis, mild chemosis, eye discharge and corneal injury. The ocular alteration, however, was reversible in nature. (Med J Indones 2003; 12: 135-41)

Effect of Roundup Ultra on atrazine degradation in soil

Tank mixing pesticides and the use of pre-packaged mixtures have become common agricultural practices. However, pesticide degradation in multi-pesticide systems is rarely evaluated. The objective of this laboratory study was to determine the effect of Roundup Ultra on atrazine degradation in soil. Based on a 2-mm glyphosate-soil interaction depth, the isopropylamine salt of glyphosate was added to Aatrex-amended and non-amended soil at rates of 0, 1 (43 mg ai kg-1), 2, 3, 4, and 5×. Treatments were incubated for 4, 8, 12, 16, 20, 24, 28, and 32 days. Atrazine degradation was significantly different among treatments at 8 days. In the 0× treatment (Aatrex only), 87% of the atrazine was degraded. During the same 8-day period, atrazine degradation in the 1, 2, 3, 4, and 5× treatments was 77%, 69%, 60%, 61%, and 52%, respectively. Atrazine degradation approached 97% for all treatments after 12 days and statistical differences were no longer observed. Atrazine degradation was inversely correlated with Roundup Ultra rate and microbial activity at 8 (r2 =0.97) and 12 days (r2 =0.92). These results indicate that Roundup Ultra stimulated microbial activity while simultaneously inhibiting atrazine degradation.

Aquatic toxicity of glyphosate-based formulations: comparison between different organisms and the effects of environmental factors

Glyphosate-based herbicides (e.g. Roundup ®) are extensively used in the aquatic environment, but there is a paucity of data on the toxicity of the formulated products and the influences by environmental factors. In this study, the acute toxicity of technical-grade glyphosate acid, isopropylamine (IPA) salt of glyphosate, Roundup ® and its surfactant polyoxyethylene amine (POEA) to Microtox ® bacterium ( Vibrio fischeri), microalgae ( Selenastrum capricornutum and Skeletonema costatum), protozoa ( Tetrahymena pyriformis and Euplotes vannus) and crustaceans ( Ceriodaphnia dubia and Acartia tonsa) was examined and the relative toxicity contributions of POEA to Roundup ® were calculated. The effects of four environmental factors (temperature, pH, suspended sediment and algal food concentrations) on the acute toxicity of Roundup ® to C. dubia were also examined. Generally, the toxicity order of the chemicals was: POEA > Roundup ® > glyphosate acid > IPA salt of glyphosate, while the toxicity of glyphosate acid was mainly due to its high acidity. Microtox ® bacterium and protozoa had similar sensitivities towards Roundup ® toxicity (i.e. IC50 from 23.5 to 29.5 mg AE/l). In contrast, microalgae and crustaceans were 4–5 folds more sensitive to Roundup ® toxicity than bacteria and protozoa. Except photosynthetic microalgae, POEA accounted for more than 86% of Roundup ® toxicity and the toxicity contribution of POEA was shown to be species-dependent. Increase in pH (6–9) and increase of suspended sediment concentration (0–200 mg/l) significantly increased the toxicity of Roundup ® to C. dubia, but there were no significant effects due to temperature change and food addition.

Glyphosate‐resistant Lolium multiflorum in Chilean orchards

Summary Lolium multiflorum (Italian ryegrass) seeds suspected of being resistant to glyphosate were collected from fruit orchards at two locations, San Bernardo (SB) and Olivar (OL), Chile, that had been treated an average of three times per year with the isopropylamine salt of glyphosate during the previous 8–10 years. Laboratory experiments were conducted for each orchard population and a susceptible population, a commercial cultivar called Tama (TM), using Petri dishes containing filter paper saturated with 5 mL of glyphosate solution (0–160 mg a.e. L−1). Pot dose–response experiments were also conducted in the greenhouse. The three L. multiflorum populations were treated with glyphosate (0.00–4.32 kg a.e. ha−1). The dose needed to reduce shoot length (Petri dish experiment) and fresh weight (pot dose–response experiment) by 50% was determined for each population. Compared with the TM population, the Petri dish experiment found that the SB and OL populations were five‐ and sixfold, respectively, more resistant to glyphosate, whereas the pot dose–response experiment found that the SB and OL populations were two‐ and fourfold, respectively, more resistant to glyphosate. These results confirm a new case of glyphosate resistance in a novel species, L. multiflorum, and correspond to the first case of glyphosate resistance reported from South America.

Mesotrione Combinations in No-Till Corn (Zea mays)1

Field studies were conducted in 1999, 2000, and 2001 to determine the effectiveness of mesotrione applied preemergence (PRE) or postemergence (POST) in no-till corn. Also, a proposed prepackage mix of mesotrione plus acetochlor (1:11 ratio of mesotrione–acetochlor) in combinations with the trimethylsulfonium salt of glyphosate (glyphosate-TMS), paraquat, and 2,4-D was investigated. Mesotrione PRE at 235 g ai/ha or greater controlled common lambsquarters, smooth pigweed, and common ragweed at least 80%. POST mesotrione at 35 g/ha and higher controlled common lambsquarters 91% or greater. Mesotrione applied POST at 140 g/ha controlled smooth pigweed greater than 97%. Common ragweed control from POST mesotrione was inconsistent, ranging from 56 to 97%. PRE and POST applications of mesotrione did not adequately control goosegrass, giant foxtail, fall panicum, johnsongrass, or cutleaf eveningprimrose. The mesotrione plus acetochlor prepackage mix plus glyphosate-TMS or paraquat controlled field pansy and ivyleaf morningglory similar to or better than did the prepackage mixture of the isopropylamine salt of glyphosate (glyphosate-IPA) plus atrazine plus acetochlor. But common ragweed control by mesotrione plus acetochlor plus glyphosate-TMS or paraquat was occasionally lower than control by the prepackage mixture of glyphosate-IPA plus atrazine plus acetochlor. Corn injury was generally less than 10% with PRE and POST mesotrione applications.Nomenclature: Acetochlor; atrazine; 2,4-D; glyphosate-Ipa (isopropylamine salt of glyphosate); glyphosate-TMS (trimethylsulfonium salt of glyphosate); mesotrione; paraquat; common lambsquarters, Chenopodium album L. #3 CHEAL; common ragweed, Ambrosia artemisiifolia L.# AMBEL; cutleaf eveningprimrose, Oenothera laciniata Hill # OEOLA; fall panicum, Panicum dichotomiflorum Michx. # PANDI; field pansy, Viola arvensis Murr. # VIOAR; giant foxtail, Setaria faberi Herrm. # SETFA; goosegrass, Eleusine indica (L.) Gaertn # ELEIN; ivyleaf morningglory, Ipomoea hederacea (L.) Jacq. # IPOHE; johnsongrass, Sorghum halepense (L.) Pers. # SORHA; smooth pigweed, Amaranthus hybridus L. # AMACH; corn, Zea mays L.Additional index words: Bleaching herbicides, burndown, nonselective herbicides, triketone herbicides.Abbreviations: COC, crop-oil concentrate; DAT, days after treatment; fb, followed by; PRE, preemergence; POST, postemergence; UAN, urea ammonium nitrate; WAT, weeks after treatment.

Flumioxazin Preplant Burndown Weed Management in Strip-Tillage Cotton (Gossypium hirsutum) Planted into Wheat (Triticum aestivum)1

Experiments were conducted at two locations in North Carolina from 1999 to 2000 to evaluate flumioxazin preplant (PP) for weed management in strip-tillage cotton planted in winter-wheat cover. Flumioxazin PP was evaluated at two rates alone and in mixture with two commonly used PP herbicides and one experimental PP herbicide. Flumioxazin PP at 71 or 105 g ai/ha tank mixed with the isopropylamine salt of glyphosate at 1.12 kg ai/ha, paraquat at 1.05 kg ai/ha, or the trimethylsulfonium salt of glyphosate at 1.12 kg ai/ha controlled common chickweed, common lambsquarters, common ragweed, Palmer amaranth, and smooth pigweed ≥ 96% 29 to 43 d after treatment (DAT). Both glyphosate formulations and paraquat alone provided ≥ 91% control of common chickweed and henbit 29 to 43 DAT, but control of common lambsquarters, common ragweed, large crabgrass, Palmer amaranth, and smooth pigweed was ≤50%. Treatments including flumioxazin injured cotton (≤ 5%) at one location. In all comparisons within a location, cotton treated with flumioxazin PP at 71 or 105 g/ha in mixture with either glyphosate formulation or with paraquat provided equivalent or higher yields than did cotton not treated with flumioxazin PP.Nomenclature: Flumioxazin; glyphosate; paraquat; common chickweed, Stellaria media L. Vill. #3 STEME; common lambsquarters, Chenopodium album L. CHEAL; common ragweed, Ambrosia artemisiifolia L. # AMBEL; henbit, Lamium amplexicaule L. # LAMAM; large crabgrass, Digitaria sanguinalis (L.) Scop. # DIGSA; Palmer amaranth, Amaranthus palmeri L. # AMAPA; smooth pigweed, Amaranthus hybridus L. # AMACH; cotton, Gossypium hirsutum L. ‘Paymaster 1218 RRBG’, ‘Paymaster 1220 RRBG’; wheat, Triticum aestivum L.Additional index words: Burndown treatment, cover crops.Abbreviations: COC, crop-oil concentrate; DAT, days after treatment; glyphosate-IP, isopropylamine salt of glyphosate; glyphosate-TM, trimethylsulfonium salt of glyphosate; PDS, postemergence-directed spray; POST, postemergence; PP, preplant; PRE, preemergence; WAP, weeks after planting.

Evaluation of Reduced Rates of Glyphosate and Chlorimuron in Glyphosate-Resistant Soybean (Glycine max)1

Field studies were conducted over 2 yr in Louisiana to evaluate entireleaf morningglory and hemp sesbania control by glyphosate isopropylamine salt applied alone at 420, 560, and 700 g ae/ha and in combination with chlorimuron at 4, 6, 9, and 11 g ai/ha. Comparison treatments included glyphosate alone at 840 g/ha, glyphosate at 1,400 g/ha plus chlorimuron at 13 g/ha, and sethoxydim at 210 g ai/ha plus chlorimuron at 11 g/ha. Entireleaf morningglory and hemp sesbania were controlled no more than 77% with glyphosate rates as high as 840 g/ha. Chlorimuron added to glyphosate increased entireleaf morningglory control in two of the three experiments to 83 to 88% and hemp sesbania control to 86 to 98%. Response to chlorimuron was not rate dependent. In most cases, glyphosate–chlorimuron mixtures did not antagonize barnyardgrass control, and increasing rate of glyphosate did not always increase control. Soybean was injured no more than 6% by chlorimuron at one location, but injury was as great as 30% at another location, which was possibly due to smaller soybean size. Differences in weed control and soybean injury among the glyphosate and glyphosate–chlorimuron combinations were not reflected in soybean yields. Because injury occurred early season, sufficient time remained for soybean to recover and produce acceptable yields. Weed control by sethoxydim plus chlorimuron was less consistent than that by glyphosate plus chlorimuron, and soybean yield in most cases was inferior to the glyphosate treatments.Nomenclature: Chlorimuron; glyphosate; sethoxydim; barnyardgrass, Echinochloa crus-galli (L.) Beauv. #3 ECHCG; entireleaf morningglory, Ipomoea hederacea var. integriuscula Gray # IPOHG; hemp sesbania, Sesbania exaltata (Raf.) Rydb. ex. A. W. Hill # SEBEX; soybean, Glycine max (L.) Merr.Additional index words: Herbicide mixtures, transgenic soybean.Abbreviation: WAT, week after treatment.

Effect of roundup ultra on microbial activity and biomass from selected soils.

Herbicides applied to soils potentially affect soil microbial activity. The quantity and frequency of Roundup Ultra [RU; N-(phosphonomethyl)glycine; Monsanto, St. Louis, MO] applications have escalated with the advent of Roundup-tolerant crops. The objective of this study was to determine the effect of Roundup Ultra on soil microbial biomass and activity across a range of soils varying in fertility. The isoproplyamine salt of glyphosate was applied in the form of RU at a rate of 234 mg active ingredient kg(-1) soil based on an assumed 2-mm glyphosate-soil interaction depth. Roundup Ultra significantly stimulated soil microbial activity as measured by C and N mineralization, as well as soil microbial biomass. Cumulative C mineralization as well as mineralization rate increased above background levels for all soils tested with addition of RU. There were strong linear relationships between C and N mineralized, as well as between soil microbial C and N (r2 = 0.96 and 0.95, respectively). The slopes of the relationships with RU addition approximated three. Since the isopropylamine salt of glyphosate has a C to N ratio of 3:1, the data strongly suggest that RU was the direct cause of the enhanced microbial activity. An increase in the C mineralization rate occurred the first day following RU addition and continued for 14 d. Roundup Ultra appeared to be rapidly degraded by soil microbes regardless of soil type or organic matter content, even at high application rates, without adversely affecting microbial activity.

Effect of Roundup Ultra on Microbial Activity and Biomass from Selected Soils

Herbicides applied to soils potentially affect soil microbial activity. The quantity and frequency of Roundup Ultra [RU; N-(phosphonomethyl)glycine; Monsanto, St. Louis, MO] applications have escalated with the advent of Roundup-tolerant crops. The objective of this study was to determine the effect of Roundup Ultra on soil microbial biomass and activity across a range of soils varying in fertility. The isoproplyamine salt of glyphosate was applied in the form of RU at a rate of 234 mg active ingredient kg−1 soil based on an assumed 2-mm glyphosate–soil interaction depth. Roundup Ultra significantly stimulated soil microbial activity as measured by C and N mineralization, as well as soil microbial biomass. Cumulative C mineralization as well as mineralization rate increased above background levels for all soils tested with addition of RU. There were strong linear relationships between C and N mineralized, as well as between soil microbial C and N (r2 = 0.96 and 0.95, respectively). The slopes of the relationships with RU addition approximated three. Since the isopropylamine salt of glyphosate has a C to N ratio of 3:1, the data strongly suggest that RU was the direct cause of the enhanced microbial activity. An increase in the C mineralization rate occurred the first day following RU addition and continued for 14 d. Roundup Ultra appeared to be rapidly degraded by soil microbes regardless of soil type or organic matter content, even at high application rates, without adversely affecting microbial activity.

Morningglory (Ipomoeaspp.) and Large Crabgrass (Digitaria sanguinalis) Control with Glyphosate and 2,4-DB Mixtures in Glyphosate-Resistant Soybean (Glycine max)1

Abstract: Glyphosate effectively controls most weeds in glyphosate-resistant soybean. However, it is sometimes only marginally effective on Ipomoea spp. A field experiment was conducted at five locations in North Carolina to determine the effects of mixing 2,4-DB with glyphosate on Ipomoea spp. control and on soybean injury and yield. The isopropylamine salt of glyphosate at 560, 840, and 1,120 g ai/ha controlled mixtures of tall morningglory, entireleaf morningglory, and red morningglory at least 96% at two locations. Mixing the dimethylamine salt of 2,4-DB at 35 g ae/ha with glyphosate did not increase control but reduced soybean yield 6%. At two other locations, 2,4-DB increased control of tall morningglory and a mixture of entireleaf morningglory and ivyleaf morningglory 13 to 22% when mixed with glyphosate at 560 g/ha, but not when mixed with glyphosate at 840 or 1,120 g/ha. Soybean yield was reduced 31% at one location and was unaffected at the other. At the fifth location, 2,4-DB increased control ...

Influence of formulated glyphosate and activator adjuvants onSclerotinia sclerotiorumin glyphosate-resistant and-susceptibleGlycine max

Abstract Two Glycine max near-isolines, GL2415 (glyphosate sensitive) and GL2600RR (glyphosate resistant), were compared for susceptibility to Sclerotinia sclerotiorum, the fungus that causes white mold disease (also known as Sclerotinia stem rot). A formulated isopropylamine salt of glyphosate (RU) at 840, 1,680, and 2,520 g ae ha−1; the RU formulation blank containing only adjuvants (RUFB) at 0.4% (v/v); organosilicone at 0.3% (v/v); a crop oil concentrate at 1.0% (v/v); and a nonionic surfactant at 0.5% (v/v) were evaluated for their influence on V5 G. max susceptibility to S. sclerotiorum in the field. The same treatments plus a formulated isopropylamine salt of glyphosate lacking adjuvants at 2,520 g ae ha−1 and a formulated trimethylsulfonium salt of glyphosate at 2,520 g ae ha−1 were applied to GL2600RR G. max in the greenhouse. Results from these studies indicated no differences in fungal growth, disease development, or G. max yield for any of these treatments. To determine if any interaction occu...