Isolated Plant Populations Research Articles

Can a seed bank maintain the genetic variation in the above ground plant population?

There are indications that a persistent seed bank can protect small and isolated plant populations from local extinction. Genetic mechanisms contributing to this phenomenon are the increase of local effective population size – and hence the decrease of genetic drift – through a reservoir of persistent seeds, and the accumulation of intergenerational genetic diversity in the seed bank. To find evidence for these mechanisms, we conducted two formal meta‐analyses. First, we analyzed 42 published habitat fragmentation studies and investigated whether the degree of genetic differentiation between fragmented plant populations was mediated by seed longevity. Second, we reviewed 13 published studies reporting the genetic diversity of both the seed bank and the above ground plants, aiming at comparing genetic diversity contained in the seed bank with the above ground vegetation. We conclude that a persistent seed bank may indeed mitigate the consequences of habitat fragmentation and protect a species from genetic drift and population genetic differentiation. We found no evidence, however, of high levels of genetic diversity accumulating in the soil seed bank. If genetic differences are present between the standing crop and the seed bank, they are very likely the result of local selection acting either directly or indirectly as a filter on the alleles present in the seed bank. We finally suggest that 1) the role of the seed bank should not be neglected in habitat fragmentation studies and 2) it is not very fruitful to continue comparing seed bank genetic diversity with above ground plant genetic diversity, unless this is performed under different selection regimes.

Distribution of myrmecochorous species over the landscape and their potential long‐distance dispersal by emus and kangaroos

ABSTRACTTopographical heterogeneity can create a mosaic of substrate types leading to the formation of isolated plant populations. Seed dispersal then becomes crucial for the colonization of such suitable but remote substrate types. We surveyed the distribution of seven elaiosome‐bearing species (myrmecochores) over 5 km2 of natural heathland in southwestern Australia. Ants are the standard means of dispersal of these species, which provide limited dispersal (usually of a few metres). Six species were associated with particular substrate types (dune or swale) and all occurred as discrete populations, on average 270–500 m apart, with closest dune edges 280 m apart. We evaluated the possible roles of emus and kangaroos as alternative agents of long‐distance seed dispersal between substrate types. Their droppings contained viable seeds of three of the target species, as well as other myrmecochores, and were evenly distributed over the substrate types. While migration of these plant species between preferred substrate types seems unlikely when considering only their standard dispersal agents (ants), it is highly likely in the presence of emus (in particular) and kangaroos that act as non‐standard dispersers. This may have important consequences for plant species conservation by increasing habitat connectivity and favouring regional persistence.

Use of genetic markers to quantify bumblebee foraging range and nest density

Bumblebees (Hymenoptera: Apidae) are important pollinators of crops and wildflowers, but many species have suffered dramatic declines in recent decades. Strategies for their conservation require knowledge of their foraging range and nesting density, both of which are poorly understood. Previous studies have mainly focussed on the cosmopolitan bumblebee species Bombus terrestris, and implicitly assume this to be representative of other species. Here we use a landscape‐scale microsatellite study to estimate the foraging range and nesting density of two ecologically dissimilar species, B. terrestris and B. pascuorum. Workers were sampled along a 10 km linear transect and 8–9 polymorphic microsatellite markers used to identify putative sisters. We provide the first published estimates of the number of colonies using a circle of radius 50 m in an agricultural landscape: 20.4 for B. terrestris and 54.7 for B. pascuorum. Estimates of nest density differed significantly between the two species: 13 km−2 for B. terrestris and 193 km−2 for B. pascuorum. Foraging ranges also differed substantially, with B. pascuorum foraging over distances less than 312 m and B. terrestris less than 625 m. Clearly bumblebee species differ greatly in fundamental aspects of their ecology. This has significant implications for the development of conservation strategies for rare bumblebees and isolated plant populations, for the management of bumblebees as pollinators, and for predicting patterns of gene flow from genetically modified plants.

Variation in the mating system of Vincetoxicum hirundinaria (Asclepiadaceae) in peripherial island populations.

Self-fertility may be selected for in small and isolated plant populations of normally outcrossing species. In addition, adaptations for self-fertility are likely to arise in island populations and in populations that are located at the border of the species range. The mating system of Vincetoxicum hirundinaria (Asclepiadaceae) is examined in island populations that are located at the northern border of the species range. Pollination experiments were conducted under glasshouse conditions with plants from four populations. The frequencies of self-fertile individuals were relatively high and did not differ among populations. Cross-pollination resulted in higher fruit set than self-pollination. However, fruit-set from self-pollination and cross-pollination did not differ in the self-fertile individuals. Interestingly, the proportion of aborted fruits was on average higher following cross-pollination than following self-pollination. No differences were observed in seed number or seed mass between self- and cross-pollinated fruits. Pollen tube growth following self- and cross-pollinations was indistinguishable. The results of this study revealed that V. hirundinaria possess a mixed-mating system in the studied island populations. Evidence was also provided for a late-acting self-incompatibility system commonly observed in Asclepiadaceae. No clear signs of inbreeding depression were observed in the early stages of development.

Molecular markers reveal a strong genetic differentiation between two European relic tree species: Zelkova abelicea (Lam.) Boissier and Z. sicula Di Pasquale, Garfì & Quézel (Ulmaceae)

Chloroplast (trnL) and ribosomal (ITS2)sequences and chloroplast DNA (PCR-RFLP andSSR) markers were analysed in two relicUlmaceae tree species: Zelkova abelicea,from Crete, and Z. sicula, from Sicily.The analysis of the plastidial trnLintron and of ITS2 ribosomal sequences revealedtheir divergence from the related speciesZ. carpinifolia, widespread in the Caucasianregion; one base substitution in the trnLintron was detected between the twoMediterranean species, thus suggesting theirrecent separation. Molecular markers(plastidial PCR-RFLP and SSR) showed an evidentgenetic differentiation between Z. siculaand Z. abelicea, the two species beingcharacterised by different haplotypes. Nowithin population variation was detected usingdifferent chloroplast markers inZ. abelicea and Z. sicula. Paleobotanicaldata proved that the genus Zelkova wasabundant and widespread in central Italy untilit became extinct in the continental part ofEurope during last glaciation events andsurvived only in two Mediterranean islands. Thesegregation of the two Mediterranean relicspecies might have occurred as a consequence ofthe strong reduction of their distribution andthe following geographic isolation. Geneticdrift may have determined the drastic reductionof within stand diversity as observed in othersmall, peripheral and geographically isolatedplant populations. The priorities forconservation programs are discussed in thelight of the different genetic resourcesrepresented by the two taxa.

Genetic Differentiation in Isolated Populations of Hakea carinata (Proteaceae)

Fragmentation of the landscape by human activity has created small, isolated plant populations. Hakea carinata F. Muell. ex Meissner, a sclerophyllous shrub, is common in isolated fragments of vegetation in South Australia. This study investigated whether habitat fragmentation has caused restrictions to gene flow between populations. Gene diversity (HT = 0.317) is average for similar species but little is held within populations (HS = 0.168) and 46.9% of gene diversity is accounted for between populations. Estimates of gene flow are NM = 0.270 (based on FST) and NM = 0.129 (based on private alleles). Populations are substantially selfing (t = 0.111). Small isolated populations appears to be a long-term evolutionary condition in this species rather than a consequence of habitat fragmentation; however, population extinctions are occurring. Conservation will require the reservation of many populations to represent the genetic variation present in the species.

Molecular Variation in Isolated Plant Populations

Abstract Isolated plant populations face a series of demographic and genetic challenges. They potentially may suffer from a loss of allelic and genotypic diversity or from inbreeding depression. The demographic stability associated with migration between components of a metapopulation is lost as fragmentation increasingly isolates populations. Such isolated populations are then increasingly subject to demographic stocasticity and to more frequent population bottlenecks. Some plant species clearly endure this challenge better than others. In studies of native plant species whose populations have long endured isolation, population numbers very often appear stable and populations often contain ample genetic variation. Of course, such observations are biased, since those species which did not accommodate isolation are presumed extinct and thus not studied.When one considers species which have recently undergone habitat fragmentation and population isolation, the ability to accommodate such change is clearly not present in many species. Such populations often show a dramatic decline in numbers as well as erosion of genetic variability. In these populations the role of somatic processes in the generation of genetic variation may assume an increased importance. Since only a few isolated studies have documented such somatic genomic changes in plants, the importance of such changes is subject to speculation.