The isokinetic interactive line protocol (ILP) appears to enhance neuromuscular function and may reduce posttraumatic stress and anxiety. However, the reliability of the protocol has not been investigated, nor has it been analyzed in relation to psychological states. A total of 165 participants took part in this study, with ages ranging from 21 to 24 years. The gender distribution was 68.4% male and 31.6% female. There was no significant difference in injury duration between males and females (z=-0.922, P=0.357). The intrarater reliability of the ILP, as indicated by the intraclass correlation coefficient, was high for both concentric and eccentric contractions of the quadriceps (ranging from 0.891 to 0.980). Likewise, the interrater reliability for these contractions was also high (range, 0.873-0.943). Additionally, during the ILP, there was a significant reduction in posttraumatic stress (t=9.795, P<0.001) and anxiety (t=5.965, P<0.001) related to knee reinjury after 2 weeks. Ultimately, the isokinetic game ILP demonstrates high intra- and interrater reliabilities and can be concluded to effectively reduce posttraumatic stress and anxiety when incorporated into retesting.