We examined the effects of 16 weeks combined exercise training on irisin, adipokines, bone metabolism markers, functional fitness and isokinetic muscle function in elderly women with osteosarcopenic obesity in elderly women. 16 participants who were 65 years of age or older were recruited and were randomly assigned into training group (n=8; TG) and control group (n=8; CG) Body composition and bone mineral density (BMD) were measured by DXA (Hologic, QDR-4500, USA). Senior fitness tests were 30-s chair stand, 30-s arm curl, chair sit-and-reach, back scratch, 8-foot up-and-go, grip strength, and 2-min step test. Isokinetic muscle strength was measured by isokinetic dynamometer (Cybex 770, USA). Biochemical parameters including irisin, leptin, adiponectin, C/Tx, 25(OH)D, osteocalcin, and PTH were measured. All data were analyzed by SAS 9.4. Two-way repeated measures ANOVA was applied. Following of combined exercise training, lumbar BMD was increased significantly (p.05) in the training group, while fat mass and lean body mass were not changed in both TG and CG. Significant improvements were obtained for 30-s chair stand (p.001), 8-foot up-and-go (p.01) and 2-min step test (p.05) in the training group. Relative peak torque in left extensor (p.001), flexor (p.01) and right flexor (p.05) at 60 degree was increased significantly between pre-and post-tests. Left extensor relative total work done (p.01), right flexor relative total work done (p.05) and left flexor relative total work done (p.05) at 180 degree were also increased significantly in TG. Combined exercise training improved significantly the level of irisin (p.05) and 25(OH)D (p.05) during intervention period. Our major findings indicate that 16 weeks combined exercise training increased level of irisin, and 25(OH)D and improved lumber BMD, functional fitness and isokinetic muscle function. We can conclude that combined exercise training is effective in preventing osteoporosis and age-related decline in muscle function.