Isozymes Research Articles

Estudo da especificidade da isoenzima MB da creatinaquinase na cirurgia cardíaca com circulação extra-corporal (fases pré, intra e pós operatória).

Estudaram-se em 6 doentes com valvulopatias reumatismais, as variações da actividade enzimática no plasma, durante e apos a cirurgia cardíaca com circulação extra-corporal, para verificar a especificidade da isoenzima MB da creatinaquinase, comparando-a com a da CK. Estudaram-se também as variações da desidrogenase láctica no mesmo período. O protocolo de colheitas escolhido, procurava dissociar as variações enzimáticas correspondentes aos tempos anteriores a miocardiotomia, das relacionadas com a lesão cirúrgica das fibras miocárdicas. Os autores verificaram existir um paralelismo evidente entre as curvas referentes a CK total e a sua isoenzima MB. Esta última elevava-se mais tardiamente, apenas durante os tempos cardíacos da intervenção, atingia o máximo no final desta e voltava mais rapidamente aos valores iniciais. A CK-MB regressava aos valores basais no 3º dia do pós-operatório e a CK-total entre o 5.° e o 7º dia. Os autores concluem que a isoenzima CK-MB é um indicador fiel e pouco sujeito a erros para detecção precoce de sofrimento das fibras musculares cardíacas. Os valores da LDH sofreram variações mais irregulares, atribuíveis a interferência de diversos factores, mas pareceram de interesse na detecção de complicações durante o pós-operatório.

Thermal Inactivation of Myrosinase from White Mustard Seeds

Myrosinases (thioglucosidases) catalyze the hydrolysis of a class of compounds called glucosinolates, of which the aglycones show various biological functions. It is often necessary to minimize the loss of myrosinase activity during thermal processing of cruciferous vegetables. Myrosinase was isolated from a popular spice, white mustard (Sinapis alba), and its thermal inactivation kinetics was investigated. The enzyme was extracted from white mustard seeds and purified by a sequential processes of ammonium sulfate fractionation, Concanavalin A-Sepharose column chromatography, and gel permeation chromatography. At least three isozymes were revealed by Concanavalin A-Sepharose column chromatography. The purity of the major myrosinase was examined by native polyacrylamide gel electrophoresis and on-gel activity staining with methyl red. The molecular weight of the major enzyme was estimated to be 171 kDa. When the consecutive step model was used for the thermal inactivation of the major myrosinase, its inactivation energy was 44.388 kJ/mol for the early stage of destruction and 32.019 kJ/mol for the late stage of destruction. When the distinct two enzymes model was used, the inactivation energy was 77.772 kJ/mol for the labile enzyme and 95.145 kJ/mol for the stable enzyme. The thermal inactivation energies lie within energy range causing nutrient destruction on heating.

Analysis of the level of T lymphocyte subsets and the relationship with the prognosis of pulmonary fibrosis in patients with paraquat poisoning

Objective: To analyze the levels of T lymphocyte subsets (CD3, CD4, CD8 and CD4/CD8) in patients with paraquat poisoning, and to explore the relationship between the changes of T lymphocyte subsets and the prognosis of pulmonary fibrosis. Methods: In October 2019, a total of 47 patients with oral 20% paraquat low water solvent poisoning in Guangzhou 12th people's Hospital from June 2018 to June 2019 were selected as the research objects. Patients were divided into early death group (16 cases died within 2 weeks) and non early death group (31 cases survived more than 2 weeks) . The non early death group was divided into pulmonary fibrosis group (23 cases) and normal lung group (8 cases) . 20 healthy people in the same period were randomly selected as the control group. The neutrophils (N) , C reaction protein (CRP) , alanine aminotransferase (ALT) , creatinine (Cr) , amylase (aAMY) , creatine kinase isoenzyme (CKMB) , pH, HCO(3)(-), blood oxygen saturation (SO(2)) and lactic acid (Lac) of patients poisoned within 3 d were examined every day. Independent sample t-test was used for inter group comparison, and paired sample t-test was used for intra group comparison. Results: Compared with non early death group, the levels of N, CRP, ALT, Cr, aAMY, CKMB and Lac in early death group increased (P<0.05) , while pH and HCO(3)(-) decreased (P<0.05) . Compared with the control group, the levels of CD3, CD4 and CD4/CD8 were decreased on the first day in the early death group and non early death group (P<0.05) , and the levels of CD3, CD4 and CD4/CD8 were decreased on the 15th day in the pulmonary fibrosis group (P<0.05) . Compared with the normal lung group, the levels of CD3, CD4 and CD4/CD8 in the pulmonary fibrosis group decreased on the 15th day (P<0.05) . Conclusion: The persistent low cellular immune function in patients with paraquat poisoning is related to the progress of pulmonary fibrosis, which is an important factor affecting the prognosis of patients with pulmonary fibrosis.

A prospective study of biochemical markers in diagnosing carcinoma breast

Introduction: Various biochemical tumour markers have been studied singly or in combination, in breast malignancies which help in diagnosing carcinoma of breast cancer.Aim: To assess the levels of enzymes Lactate dehydrogenase (LDH), Gamma glutamyl trans peptidase (GGT), and superoxide dismutase (SOD) in patients with newly diagnosed carcinoma breast and study if there is any clinical correlation of the levels of these enzymes with the presence of early untreated breast carcinoma.Materials and Method: This is prospective study done during the period February 2018 to Jan 2019.In Government Pudukkottai medical college. It was carried out in two groups, namely apparently healthy 27 females controls and 50 females with newly diagnosed carcinoma breast.Results: The imbalance between free radicals and the anti-oxidants in the causation of breast cancer is being researched. Free radicals has been implicated in initiation and promotion of carcinogenesis. This study was undertaken to evaluate if the levels of biochemical markers LDH and GGT, and the level of antioxidant enzyme SOD are altered in cases with carcinoma breast, when compared to the levels in apparently normal controls.In humans, three forms of superoxide dismutase are present. SOD1 is located in the cytoplasm, SOD2 in mitochondria and SOD3 in extracellular matrix. SOD causes detoxifies super oxide to hydrogen peroxide. LDH is a glycolytic enzyme with five iso enzymes. The level of LDH is increased in various malignancies. GGT helps in transportation of amino acids in the cells.Conclusion: The levels of LDH and GGT were found to be significantly elevated in the cases, whereas the levels of SOD were found to be significantly lowered in the cases. The SOD levels were lower in cases with stage-3 carcinoma breast than in cases with stage-2 carcinoma breast.

EXTRACTION AND PURIFICATION OF BETA-GALACTOSIDASE FROM LOCAL ALMOND AND ITS USE FOR LACTOSE INTOLERANCE TREATMENT

This study was aimed, extraction and purification of beta-Galactosidase from local almond(Amygdalus communis) for lactose intolerance treatment. The best one among 10 methods method of the extraction was using sodium phosphate buffer at 0.2 molar. Which was achieves the highest specific activity amounted to 3.66unit/mg protein. Then, partial purification of enzyme was done using five methods. The highest specific activity was obtained using the method of precipitation with ammonium sulphate at 30-70% since the specific activity was 15.85units/mg protein. Which represented the best way to precipitation the enzyme. Three iso enzymes were obtained. One of them was taken for its high specific activity(20.10units/mg protein) and ion exchange chromatography was used and followed by gel filtration technique using sephadex-100 column to increase purification. The specific activity was increased to 21.95units/mg protein. Lactose hydrolysis efficiency test was performed and the purified enzyme showed high efficiency in standard lactose hydrolysis test.

Recent clinical advances of glucokinase activators in the treatment of diabetes mellitus type 2.

Glucokinase (GK) is an isozyme of hexokinase that catalyzes the phosphorylation of glucose. GK is present in many organs of the human body, including the liver and pancreas. GK plays an important role in promoting the synthesis of hepatic glycogen and balancing postprandial blood glucose. Mutations in the GK gene can result in the inclusion of maturity-onset diabetes of the young (MODY2) and permanent neonatal Diabetes mellitus (PNDM). Glucokinase activators (GKAs) are a class of type 2 diabetes drugs designed for this target. In various animal models of type 2 diabetes, GKAs have been shown to have the ability to decrease blood glucose, and some GKAs also have the ability to stimulate β cell proliferation. However, due to the induction of hypoglycemia and increased liver burden, many candidates stopped in phase II clinical trials. Recently, dorzagliatin has reached the primary endpoint of phase III clinical trial, which can repair the core function of GK as a blood glucose sensor, and can delay or even reverse islet β -cell damage and functional decline. This provides a promising prospect for the study of GKAs as candidates for the treatment of type 2 diabetes. This review summarizes the clinical advances in GKAs.

The effect of myocardial infarction-associated transcript 2 (Mirt2) and miR-101 on sepsis-induced myocardial injury in rats.

The aim of this study was to explore the effect of myocardial infarction associated transcript 2 (Mirt2) and miR-101 on sepsis-induced myocardial injury in rats and its mechanism. Sprague Dawley (SD) rats were divided into sham group, CLP group, CLP + adeno-associated virus (AAV)-lncRNA Mirt2 group, CLP + AAV-NC group and CLP + AAV-lncRNA Mirt2 + agomiR-101 group. Rat CLP model was constructed. PanoViewb1500 was used to detect left ventricular systolic blood pressure (LVSP), left ventricular end diastolic blood pressure (LVEDP), ejection fraction (EF) and fraction shortening (FS) values. Thereafter, Mirt2 and miR-101 expression levels were detected by real-time polymerase chain reaction (RT-PCR), myocardial pathological damage was detected by hematoxylin-eosin (HE) staining, and Interleukin-1β (IL-1β) expression was by immunohistochemical staining. Next, enzyme linked immunosorbent assay (ELISA) was performed to detect the levels of serum inflammatory factors, tumor necrosis factor α (TNF-α), IL-1β, Interleukin-6 (IL-6), myeloperoxidase (MPO), cardiac troponin I (cTn I) and creatine kinase isoenzyme (CK-MB). After that, Western blot was used to detect the expression of inflammatory factors and Phosphatidylinositol-3-kinases (PI3K)/protein-serine-threonine kinase (AKT) signaling pathway. Finally, Dual-Luciferase reporter gene assay was conducted to detect the relationship between Mirt2 and miR-101. Compared with those in the sham group, the cardiac function of the rats in the CLP group was significantly deteriorated, the cardiac structure was disordered, and the expression of pro-inflammatory related factors was significantly increased. Compared with those in CLP group, the cardiac function of the CLP + AAV-lncRNA Mirt2 group was alleviated, the PI3K/AKT signaling pathway was activated, the cardiac structure was slightly disordered, and the expression of pro-inflammatory related factors was reduced. To some extent, miR-101 could directly inhibit the effect of Mirt2. Mirt2 can silence miR-101 and inhibit myocardial inflammatory response in sepsis rats through the PI3K/AKT signaling pathway, thus improving cardiac structure and function.

Hematopoietic Stem Cells

The small population of pluripotent hematopoietic stem cells (PHSCs) in the bone marrow consists of short-term reconstituting cells (STRCs) and long-term reconstituting cells (LTRCs), based on how quickly the transplanted cells can produce progeny in an irradiated recipient. They can be “purified” using a combination of cell size; density; fluorescent dye uptake; resistance to cytotoxic chemicals; and cell-surface markers including Thy 1.1 (T), Sca-1 (S), c-kit (K), lineage (L), CD38 (38), and CD34 (34). Using five-color fluorescence-activated cell sorting the long term, very primitive mouse LTRCs are L-/lo, S+, K+, 38+, 34-, and appear to mature to L-/lo, S+, K+, 38+, 34+ cells and then to L-/lo, S+, K+, 38-, 34+; thus, STRCs acquire CD34 and lose CD38 on maturation from CD34- LTRCs. CD34 has been used to isolate PHSCs for human transplantation studies; therefore, the LTRC may be lost during this procedure. Experimental transplantation studies indicate that the best reconstitution occurs when both cell populations are present, the more mature cells activating the immature cells after myeloablation, whereas the mature cells provide negative control in normal animals. Functionally the type of assay that has been most widely used for the quantitation of mouse stem cells is the in vivo repopulating assay. Different numbers of donor cells are combined with a standard number of normal bone marrow cells. The normal cells protect against the immediate effects of myeloablation and compete with the donor stem cells. The proportions of mature cells derived from the donor stem cells are determined by the detection of a donor-specific marker, such as an isoenzyme, Y-chromosome, or congenic antigen. Similarly, using limiting dilution transplant of a donor test population of cells and a standard number of stem cell—compromised serially transplanted cells, the relative contribution of the donor cells is measured as a competitive repopulating unit. Finally, the repopulating stem cell unit assay using complete myeloablation and busulfan-treated bone marrow radioprotective support cells provides comparatively rapid and sensitive detection of the very small numbers of LTRCs present in limiting dilution transplants. This procedure utilizes busulfan because it appears preferentially stem cell toxic, and it provides radioprotective support cells that are unable to compete effectively with normal donor stem cells in the population under investigation. Stem cells are selected based on their ability to produce both lymphoid and myeloid repopulation in severely ablated mice, rather than competitive ability.

Clinical analysis of risk factors for severe patients with novel coronavirus pneumonia

Objective To analyze the clinical features of patients with coronavirus disease 2019 (COVID-19) in Shanghai and to investigate the risk factors for disease progression to severe cases. Methods The clinical data of 292 adult patients with COVID-19 hospitalized in Shanghai Public Health Clinical Center from January 20, 2020 to February 10, 2020 were retrospectively analyzed, including 21 severe patients and 271 mild patients. The demographic characteristics, epidemiological history, history of underlying diseases and laboratory examinations were compared between the two groups. Measurement data were compared using t test or Mann-Whitney U test. The count data were compared using hi-square test. The binary logistic regression equation was used to analyze the risk factors for the progression of patients to severe cases. Results Among the 292 patients, 21 were severe cases with the rate of 7.2% (21/292). One patient died, and the mortality rate was 4.8% in severe patients. The severe patients aged (65.0±15.7) years old, 19 (90.5%) were male, 11 (52.4%) had underlying diseases, 7 (33.3%) had close relatives diagnosed with COVID-19. The mild patients aged (48.7±15.7) years old, 135 (49.8%) were male, 74 (27.3%) had underlying diseases, 36 (13.3%) had close relatives diagnosed with COVID-19. The differences between two groups were all significant statistically (t=-4.730, χ2=12.930, 5.938 and 4.744, respectively, all P<0.05). Compared with the mild patients, the levels of absolute numbers of neutrophils, alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, creatinine, serum cystatin C, C reactive protein (CRP), procalcitonin , D-dimer, pro-B-type natriuretic peptide (proBNP), serum myoglobin, creatine kinase (CK), creatine kinase isoenzyme (CK-MB), serum troponin I (cTnI) in severe patients were all significantly higher (U=2 091.5, 1 928.0, 1 215.5, 729.0, 1 580.5, 1 375.5, 917.5, 789.5, 1 209.0, 1 434.0, 638.0, 964.5, 1 258.0 and 1 747.5, respectively, all P<0.05), while the levels of lymphocyte count, albumin, transferrin, CD3+ T lymphocyte count, CD8+ T lymphocyte count and CD4+ T lymphocyte count in severe patients were all significantly lower (U=1 263.5, t=4.716, U=1 214.0, 962.0, 1 167.5 and 988.0, respectively, all P<0.05). Further logistic regression analysis showed that the albumin (odds ratio (OR)=0.806, 95%CI 0.675-0.961), CRP (OR=1.016, 95%CI 1.000-1.032), serum myoglobin (OR=1.010, 95%CI 1.004-1.016), CD3+ T lymphocyte count (OR=0.996, 95%CI 0.991-1.000) and CD8+ T lymphocyte count (OR=1.006, 95%CI 1.001-1.010) at admission were independent risk factors for the progression of COVID-19 patients to severe illness (all P<0.05). Conclusions Severe cases of patients with COVID-19 in Shanghai are predominantly elderly men with underlying diseases. Albumin, CRP, serum myoglobin, CD3+ T lymphocyte count and CD8+ T lymphocyte count could be used as early warning indicators for severe cases, which deserve more clinical attention. Key words: Coronavirus infection; Pneumonia; 2019 novel coronavirus; Severe case; Risk factor

Anaesthesia effect of ropivacaine in different concentrations on intermuscular sulcus brachial plexus block under ultrasound guidance

Objective To explore the anesthetic effect of different concentrations of ropivacaine in intramuscular sulcus brachial plexus block under ultrasound guidance. Methods From April 2016 to April 2018, 120 patients with upper limb orthopedic surgery who received muscle under the ditch brachial plexus block guided by ultrasound in Jinhua Hospital of Traditional Chinese Medicine were randomly divided into group A (40 cases, application of 0.375% ropivacaine), group B (40 cases, application of 0.50% ropivacaine), group C (40 cases, application of 0.75% ropivacaine). The anesthetic effect, onset time of sensory block, onset time of motion block, duration of analgesia, recovery time of motion block, serum troponin level, creatine kinase isozyme level and incidence of adverse reactions in the three groups were compared. Results The excellent and good anesthesia rate of group A, group B and group C was 100.00%, and there was no statistically significant difference among the three groups (P>0.05). The onset time of sensory block[(5.29±1.32)min], the onset time of motion block[(7.42±1.36)min] in group C were shorter than those in group A[(9.32±1.52)min, (13.32±2.46)min] and group B[(7.89±1.63)min, (9.12±2.20)min](F=4.652, 6.365, all P 0.05). Conclusion With the increase of ropivacaine concentration, the onset time of anesthesia is shorter and the analgesic time is longer, but the incidence of adverse reactions is also higher.In a comprehensive consideration, 0.50% ropivacaine can be applied to patients with intermuscular sulcus brachial plexus block under ultrasound guidance. Key words: Anesthesia; Brachial plexus; Ultrasonography; Intermuscular groove; Analgesia; Ropivacaine

Effect of acupuncture on hemodynamics in rats with chronic myocardial ischemia during induction of anesthesia with propofol

Objective To investigate the effect of acupuncture on hemodynamics in chronic myocardial ischemia rats during induction of anesthesia. Methods Forty five Sprague-Dawley rats, weight 200-250 g, were divided into three groups according to a random number table (n=15): a normal control group (group NC), a chronic myocardial ischemia group (group HR) and an acupuncture group (group A-HR). After acupuncture intervention of myocardial ischemia modeled animals for four weeks, the rats received induction of anesthesia with 0.1% propofol at 9 mg/kg via the jugular vein. Subsequently, the mean arterial pressure (MBP), left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP), the maximal rate of rise/decline of left ventricula pressure (±dp/dt), heart rate, and superoxide dismutase (SOD), malondialdehyde (MDA), creatine kinase isoenzyme (CK-MB) and the morphological changes of myocardial tissues were measured. Results Compared with those in group NC, LVSP, ±dp/dt and MBP remarkably declined in group HR (P<0.05), while LVEDP and heart rate significantly increased in group HR (P<0.05). Compared with group HR, group A-HR presented significantly increased LVSP, +dp/dt and MBP (P<0.05) and significantly decreased heart rate (P<0.05). Compared with group HR, group A-HR produced remarkable decreases in MDA and CK-MB (P<0.05) and marked increases in SOD (P< 0.05). Conclusions Acupuncture pretreatment of rats with myocardial ischemia can regulate and control hemodynamic stabilities during induction of anesthesia, which may be done through inhibiting lipid peroxidation to improves cardiac micro-circulation and reducing the injury of cardiac muscles. Key words: Acupuncture; Myocardial ischemia; Propofol; Anesthesia induction; Hemodynamics

Role of nuclear factor-erythroid 2-related factor 2 in diabetic rats with resveratrol preconditioning against myocardial isch- emia-reperfusion injury

Objective To observe the effect of nuclear factor-erythroid 2-related factor 2 (Nrf2) in diabetic rats preconditioning with resveratrol (Res) against myocardial ischemia/reperfusion injury (MI/RI). Methods Healthy adult male SD rats weighing 200- 220 g and aged from 4 to 5 months were fed with high-fat diet for 4 weeks and then intraperitoneally injected with streptozotocin (STZ) 30 mg/kg to establish a diabetic rat model. Thirty successfully modeled rats were divided into three groups according to a random number table (n=10): a sham operation group (a Sham group), a myocardial ischemia/reperfusion group (an MI/R group) and a myocardial ischemia/reperfusion+resveratrol group (an MI/R+Res group). A rat model of MI/RI was established by ligation of the left anterior descending coronary artery for 30 min before reperfusion for 120 min. In the MI/R+Res group, 15 mg/kg Res was intraperitoneally injected 7 d before establishment of the MI/RI model, once a day for 7 consecutive days. The Sham and MI/R groups were intraperitoneally injected with an equal volume of dimethyl sulfoxide (DMSO) and PBS mixture. Left ventricular ejection fraction (LVEF) and left ventricular short axis shortening (FS) were measured by B-mode ultrasonography. Five rats were sacrificed 120 min after reperfusion. Myocardial infarction size was measured by 2, 3, 5-triphenyl-2H-tetrazolium chloride (TTC) staining. Another five rats were sacrificed to collect blood samples from the abdominal aorta for measurement of lactate dehydrogenase (LDH), a marker of myocardial injury, and creatine kinase isoenzyme (CK-MB). Their myocardial tissues were collected to determine the levels of superoxide dismutase(SOD), malondialdehyde (MDA) and reactive oxygen species (ROS) by enzyme-linked immunosorbent assay (ELISA). The contents of Nrf2 were observed by immunohistochemistry. The expression of Nrf2, n-Nrf2 and heme oxygenase-1 (HO-1) were determined by Western blot. Results Compared with those in the Sham group, LDH, CK-MB concentration, ROS and MDA content in the MI/R and MI/R+ Res groups significantly increased while LVEF, FS, and SOD activity in the MI/R and MI/R+Res groups significantly decreased. Mean-while, the myocardial infarction sizes in the MI/R and MI/R+Res groups were increased, and the expression of Nrf2, n-Nrf2 and HO-1 protein was down-regulated (all P<0.05). Compared with those in the MI/R group, LDH, CK-MB concentration, ROS and MDA content significantly decreased in the MI/R+Res group, while LVEF, FS and SOD activity significantly increased in the MI/R+Res group. Also, in the MI/R+Res group, the myocardial infarction size decreased while the expression of Nrf2, n-Nrf2 and HO-1 protein increased (all P< 0.05). Conclusions Resveratrol preconditioning relieves myocardial injury in diabetic rats with ischemia/reperfusion, which may attribute to activation of Nrf2. Key words: Resveratrol; Transcription factor-related factor 2; Heme oxygenase-1; Diabetes; Myocardial ischemia/reperfusion

Clinical significance of the monocyte to high-density lipoprotein cholesterol ratio in the assessment of imperfect ST-segment resolution in elderly patients with acute ST-elevation myocardial infarction after percutaneous coronary intervention

Objective To investigate the clinical significance of the monocyte count/high-density lipoprotein cholesterol ratio(MHR)in evaluating imperfect ST-segment resolution in elderly patients with acute ST-elevation myocardial infarction(STEMI)after percutaneous coronary intervention(PCI). Methods This was a retrospective cohort study.A total of 274 elderly patients with STEMI underwent PCI in our hospital from December 2015 to December 2018 were enrolled.Based on the extent of the ST-segment resolution of the postoperative electrocardiogram, patients were divided into an imperfect ST-segment resolution group(observation group, n=79)and a favorable ST-segment resolution group(control group, n=195). General clinical data were compared between the two groups, and logistic regression equation was used to analyze the association of MHR with ST-segment resolution.Receiver operating characteristic(ROC)curve was performed to assess the predictive value of MHR for imperfect ST-segment resolution. Results Compared with patients in the control group, patients in the observation group were associated with a significantly higher proportion of anterior wall myocardial infarction and heart failure(≥Killip 2), A longer duration of chest pain to balloon expansion, higher levels of creatine kinase isoenzyme, N-terminal pro-brain natriuretic peptide, hypersensitive C-reactive protein, blood sugar, blood uric acid, fibrinogen, triglyceride and mononuclear cell count, and lower levels of high density lipoprotein cholesterol and lymphocyte count(all P<0.05). Meanwhile, there was a significant difference in MHR between the observation group and the control group [(0.75±0.22)vs.(0.48±0.19), t=9.831, P=0.001]. Multivariate Logistic regression analysis showed that MHR was an independent risk factor for imperfect ST-segment resolution(OR=1.950, 95%CI: 1.646-5.430, P=0.003)and ROC curve showed the threshold value of MHR at 0.67, the area under the curve at 0.867, the sensitivity at 79.72%, and the specificity at 79.61%. Conclusions MHR may be an independent risk factor and a good predictive index for imperfect ST-segment resolution in elderly patients with STEMI after PCI. Key words: Monocytes; Lipoproteins HDL; Myocardial infarction

Expression of lncRNA-ANRIL in patients with coronary heart disease before and after treatment and its short-term prognosis predictive value.

This study was designed to explore the expression of LncRNA-ANRIL in patients with coronary heart disease before and after treatment and its short-term survival prediction value. Eighty-three patients with coronary heart disease who came to our hospital undergoing interventional therapy were selected as a research group, 81 healthy volunteers who came to our hospital for normal physical examination during the same period were selected as a control group, and LncRNA-ANRIL of subjects in the two groups before and after treatment were detected by RT-PCR. Levels of Gensini score, lactate dehydrogenase (LDH), creatine kinase isoenzyme (CK-MB), creatine kinase (CK), and BNP of patients in research group before treatment were evaluated and detected, and the correlation between those and LncRNA-ANRIL was analyzed. Then, the effective treatment of LncRNA-ANRIL for patients with coronary heart disease and the predictive value of poor prognosis were analyzed. The expression of LncRNA-ANRIL in patients with coronary heart disease was lower than that of normal subjects (p<0.05), and the expression levels of Gensini score, LDH, CK-MB, CK, and BNP gradually increased with the increased number of their diseased vessels (p<0.05). The expression of LncRNA-ANRIL was negatively correlated with expressions of Gensini score, LDH, CK-MB, CK, and BNP (p<0.05); ROC of LncRNA-ANRIL in predicting effective treatment, and poor prognosis of patients with coronary heart disease was over 0.9, as well as smoking; LncRNA-ANRIL, Gensini score, LDH, CK-MB, CK, and BNP were independent risk factors for the occurrence of MACE. LncRNA-ANRIL expresses low in the serum of patients with coronary heart disease, and it has high predictive value both for effective treatment and poor prognosis of them. Also, lncRNA-ANRIL is also an independent risk factor for their poor prognosis.

Investigação da isoenzima a-esterase em Aedes aegypti de dois municípios de Mato Grosso

Investigação da isoenzima a-esterase em Aedes aegypti de dois municípios de Mato Grosso

Clinical study of Warfarin combined with Aspirin in the treatment of Kawasaki disease complicated with multiple medium and giant coronary artery aneurysms

Objective To investigate the safety and efficacy of Warfarin combined with Aspirin in the treatment of multiple medium and giant coronary artery aneurysms in Kawasaki disease(KD). Methods Clinical and follow-up data of 45 children diagnosed with KD complicated with multiple medium-sized and giant coronary artery aneurysms from April 2014 to December 2018 at Guangzhou Women and Children′s Medical Center were collected.These children were divided into 2 groups.A total of 31 cases received regular oral Warfarin combined with Aspirin called experimental group.There were 14 patients treated with oral Aspirin and Clopidogrel called control group.General information, laboratory examination, electrocardiogram, echocardiography, outcome and bleeding complications of the 2 groups were analyzed retrospectively. Results (1) In experimental group, there were 22 patients found thrombosis under echocardiography.The 10 patients′ thrombosis disappeared, 5 patients′ thrombosis reduced, and 2 patients′ increased after treatment.In control group, there were 5 cases found thrombosis.The 2 cases′ thrombosis reduced and 3 cases′ throm-bosis increased.The number of thrombosis in experimental group was significantly reduced, and the number of new thrombosis was less than that in control group (χ2=6.454, P<0.05). (2) The number of coronary artery aneurysms in experimental group increased slowly than that in control group [12.90%(4/31 cases)vs.14.28%(2/14 cases)]. (3) The number of coronary artery aneurysms in experimental group decreased rapidly than that in control group [23.91%(11/46 cases)vs.10.00%(1/10 cases)]. (4) The number of cases of tumor retraction in experimental group was more than that in control group [74.19%(23/31 cases)vs.42.85%(6/14 cases)]. (5) During the followed-up, there was no abnormality in the blood phosphokinase isozyme and troponin, no abnormality in the electrocardiogram and echocardiogram, no ventricular enlargement and abnormal ventricular wall movement, and the ejection fraction value was within the normal range.No active bleeding and no death occurred in the two groups. Conclusions Warfarin combined with Aspirin is very safe and effective in the treatment of KD coronary tumor, it can reduce thrombosis effectively.Compared with oral Aspirin and Clopidogrel, Warfarin combined with Aspirin can reduce the number of multiple medium-sized and large coronary artery aneurysms and reduce the diameter of coronary artery aneurysms. Key words: Warfarin; Aspirin; Kawasaki disease; Coronary artery aneurysm; Giant coronary artery aneurysm

Effect analysis of diazepam combined with phenobarbital in the treatment of febrile convulsions in children

Objective To investigate the clinical effect of diazepam combined with phenobarbital in the treatment of febrile convulsions in children. Methods From January 2015 to July 2017, 100 children with febrile convulsions in the Women's and Children's Hospital of Ningbo were selected and divided into two groups by random number table, with 50 cases in each group.The control group was treated with diazepam, while the observation group was treated with diazepam combined with phenobarbital.The clinical efficacy, anticonvulsive time, myocardial enzyme index, hypersensitive C-reactive protein and incidence of adverse reactions were compared between the two groups. Results (1)The total clinical effective rate of the observation group(98%) was higher than that of the control group(χ2=4.891, P<0.05), and the time of stopping convulsion[(20.92±4.49)min] was shorter than that of the control group(t=5.443, P<0.05). (2)After treatment, the creatine kinase isoenzymes[(25.93±12.76)U/L], phosphocreatinase[(189.47±7.35)U/L], hydroxybutyrate dehydrogenase[(276.73±12.85)U/L], lactate dehydrogenase[(305.94±13.27)U/L], hypersensitive C-reactive protein[(5.04±1.65)ng/L] in the observation group were lower than those in the control group(t=4.961, 5.333, 5.901, 6.160, 5.354, all P<0.05). (3)During the treatment period, no obvious adverse reactions occurred in both two groups. Conclusion The combination of diazepam and phenobarbital can effectively arrest febrile convulsions in children, improve the clinical efficacy, protect myocardial cells, it has fewer adverse reactions, it is safe and reliable. Key words: Hospitals, pediatric; Seizures, febrile; Isoenzymes; C-reactive protein; Treatment outcome; Diazepam; Phenobarbital

Survival and prognostic analysis of adult nonclear cell renal cell carcinoma

Objective To analyze the prognostic factors of adult nonclear cell renal cell carcinoma (nccRCC). Methods The clinical data of 286 patients with pathologically diagnosed one specific type of nccRCC after radical nephrectomy and nephron sparing surgery(NSS) in the affiliated hospital of Qingdao university followed up from January 2012 to January 2019 were retrospectively analyzed.There were 159 males and 127 females. Their age ranged from 17 to 81 years old, with an average age of 53. Based on the AJCC combination stage, 218 cases were in stage Ⅰ, 56 cases were in stage Ⅱ, 9 cases were in stage Ⅲ, 3 cases were in stage Ⅳ. Assay indicators were collected, including lymphocyte percentage(LY%)(31.5±10.5), neutrophil-lymphocyte ratio(NLR)(2.6±2.8), albumin(40.9±4.7)g/L, prealbumin(255.0±74.3)mg/L, lactate dehydrogenase (LDH)(201.0±174.0)U/L, creatine kinase isoenzyme (CK-MB)(20.0±62.1)U/L, total cholesterol(4.9±1.0)mmol/L, blood urea nitrogen/creatinine (BUN/Cr)(12.9±9.9), blood glucose(5.4±1.3)mmol/L, triglyceride(1.4±1.1)mmol/L, low-density lipoprotein cholesterol (LDL-C)(2.9±0.8)mmol/L. The optimal cut-off value of the above indexes were obtained by the receiver operating characteristic curve(ROC) in the SPSS software, and difference between high cut-off and low cut-off divided basing on the optimal cut-off value were evaluated respectively. The prognostic factors of adult nccRCC were evaluated by univariate and multivariate Cox proportional hazards regression analysis. Kaplan-Meier survival curve was used to study the survival relationship. The log-rank test were used to compare survival rate in two groups. The prognostic factors of nccRCC were analyzed after the results above were presented. Prognostic factors in renal chromophobe cell carcinoma and papillary cell carcinoma were analyzed by the same method. Results The 286patients were followed up from 1 to 87 months, with an average of 43.9 months. The 3-year and 5-year survival rates were 93.8% and 89.3%, respectively. Results of univariate and multivariate Cox regression model revealed that AJCC combined staging (HR=2.38, 95%CI1.48-3.83), LDH(HR=2.99, 95%CI1.16-7.69), blood glucose (HR=4.13, 95%CI 1.74-9.78), CK-MB (HR=3.85, 95%CI1.63-9.08) were independent prognostic factors of nccRCC. NLR(HR=8.28, 95%CI1.66-41.35) and LDH(HR=9.82, 95%CI2.94-32.82) were the independent prognostic factor in the renal chromophobe cell carcinoma subgroup and the papillary renal cell carcinoma subgroup, separately. Conclusions AJCC combination stage, LDH, blood glucose and CK-MB are independent prognostic factors of adult nccRCC. Patients with low LDH, hypoglycemia, CK-MB and early AJCC stage have better prognosis. NLR is an independent predictor of renal chromophobe cell carcinoma, and the low NLR group has a better prognosis and higher survival rate. LDH is an independent predictor of papillary renal cell carcinoma and low LDH is beneficial to patients' prognosis. NLR and LDH can be used as a prognostic indicator for clinical evaluation in renal chromophobe cell carcinoma and papillary renal cell carcinoma, respectively. Key words: Kidney neoplasms; Nonclear cell renal cell carcinoma; Pathology; Prognosis

Effects of ulinastatin combined with furosemide on myocardial enzymes, renal function and adverse reactions in patients with acute renal failure after cardiopulmonary resuscitation

Objective To explore the effects of ulinastatin combined with intravenous pump injection of furosemide on myocardial enzymes, renal function and adverse reactions in patients with acute renal failure(ARF) after cardiopulmonary resuscitation(CPR). Methods From January 2016 to May 2018, 117 patients with ARF after successful CPR in the Second People's Hospital of Hefei were divided into observation group(n=59) and control group(n=58) using simple random method.The control group received routine treatment, while the observation group added ulinastatin combined with intravenous pump infusion of furosemide.Myocardial enzymology, renal function, metabolism, inflammatory index, adverse reaction and survival rate were compared. Results Three and 7 days after treatment, the hydroxybutyrate dehydrogenase(HDBH), isoenzymes of creatine kinase isoenzyme(CK-MB) and mitochondrial aspartate aminotransferase isoenzyme(m-AST) were decreased in the two groups, and compared with the control group, which of the observation group were lower [HDBH: (231.42±31.15)U/L vs.(268.59±34.87)U/L; F=12.01, P=0.00; CK-MB: (32.38±4.15)ng/mL vs.(37.57±3.96)ng/mL; F=15.12, P=0.00; m-AST: (25.18±4.24)U/L vs.(33.92±5.60)U/L; F=12.36, P=0.00]. After treatment, the blood urea nitrogen(BUN), 24h urine protein quantity and creatinine(Cr) in the two groups increased firstly and then decreased, and compared with the control group, those of the observation group were lower[BUN: (7.02±1.66)mmol/L vs.(8.47±1.38)mmol/L; F=11.24, P=0.00; Cr: (82.69±9.87)μmol/L vs.(90.18±10.37)μmol/L; F=10.39, P=0.00; 24h urine protein quantity: (15.43±2.17)mg vs.(18.62±3.14)mg; F=11.06, P=0.00]. Three and 7 days after treatment, the levels of blood lactic acid(Lac), hypersensitive C-reactive protein(hs-CRP) and tumor necrosis factor-α(TNF-α) were decreased in the two groups, and compared with the control group, those of the observation group were lower [Lac: (1.18±0.27)mmol/L vs.(2.17±0.34)mmol/L, F=16.29, P=0.00; hs-CRP: (4.89±0.81)mg/L vs.(6.17±1.10)mg/L, F=13.41, P=0.00; TNF-α: (72.18±7.62)ng/L vs.(83.16±7.79)ng/L, F=11.39, P=0.00]. The incidence rates of adverse reactions in the observation group and the control group were 5.09% and 1.72%, respectively, the difference was statistically significant(χ2=0.24, P=0.62). The survival rates in the observation group and the control group were 50 cases (84.75%) and 39 cases (67.24%) respectively, the difference was statistically significant(χ2=4.92, P=0.02). Conclusion Intravenous pump injection of furosemide combined with ulinastatin can protect the heart and kidney, and improve the survival rate of patients with ARF after CPR and it is worthy of popularizing. Key words: Cardiopulmonary resuscitation; Renal insufficiency; Blood urea nitrogen; Creatinine; Blood lactic acid; Ulinastatin; Furosemide

Xuebijing injection attenuates cardiac injury in patients undergoing cardiopulmonary bypass

Objective To estimate the effect of Xuebijing(XBJ) injection on cardiac injury associated with cardiopulmonary bypass (CPB). Methods Sixty CPB patients undergoing cardiac surgery were randomly divided into a control group and an XBJ group (n=30). Patients in the XBJ group received XBJ 12 h prior to operation, at the beginning of operation, and 12 h after the second injection, while those in the control group was given normal saline alone. Their arterial blood gas analysis, inflammatory factors in peripheral blood and related indicators of oxidative stress were measured before the first injection of XBJ(as a baselin value) and after surgery 0, 12, 24, 48 h. The time of mechanical ventilation in intensive care unit (ICU), and treatment duration and discharge time were recorded. Results Compared with the control group, XBJ greatly increased arterial partial pressure of oxygen/fraction of inspiratory oxygen (PaO2/FiO2) and interleukin(IL)-10 amounts, but decreased the levels of troponin I (TnI), creatine kinase isoenzyme (CK-MB), malonaldehyde (MDA), 8-isoprostane, tumor necrosis factor-α (TNF-α), IL-1β and IL-8 after operation (all P<0.05). The XBJ group presented shorter time of mechanical ventilation in ICU, treatment duration and discharge time than the control group, without statistical differences. Conclusions XBJ can protect cardiac injury induced by CPB, which may be associated with the down-regulation of inflammatory mediators and oxidative response. Key words: Xuebijing injection; Cardiopulmonary bypass; Cardiac injury