Isocaloric Supplementation Research Articles

Protein supplementation delivered alone or in combination with presumptive azithromycin treatment for enteric pathogens did not improve linear growth in Bangladeshi infants: results of a cluster-randomized controlled trial.

Protein requirements established for healthy populations may be insufficient to support healthy growth in infants consuming largely cereal-based complementary foods and frequently exposed to enteric pathogens. To assess independent and combined effects of protein supplementation and antibiotic treatment on linear growth of infants from 6-12 months of age. We conducted a 2 x 4 factorial cluster-randomized trial in northwestern Bangladesh, allocating 566 clusters to masked azithromycin (10 mg·kg-1 * 3 d) or placebo at 6 and 9 months of age, and unmasked delivery of an egg white protein-rich blended food supplement (250 kcal; 10 g added protein), a rice-based isocaloric supplement, egg, or nutrition education from 6-12 months. We measured length at 6 and 12 months. For this cluster-level intention-to-treat analysis of the 2 x 2 antibiotic and protein interventions, we used multiple linear or log-binomial regression with generalized estimating equations to assess changes in length-for-age Z (LAZ) score and stunting (LAZ<-2), respectively. We enrolled 2055 infants (283 clusters) and included 1821 infants (281 clusters) with complete anthropometry data at 6 and 12 months in our analysis. There were no significant interactions between the protein and antibiotic interventions for any outcomes. Independently, protein supplement did not improve LAZ (β = 0.05; 95% CI: 0.00, 0.11; P = 0.07) or reduce stunting (prevalence ratio (PR) = 1.12; 95% CI: 0.85, 1.49; P = 0.41) compared to the isocaloric supplement. The antibiotic intervention had no effect on LAZ (β = -0.05; 95% CI: -0.11, 0.01; P = 0.09) or stunting (PR = 0.99; 95% CI: 0.75, 1.31; P = 0.96), relative to the placebo. Supplementation to increase intakes of high-quality protein, provided with or without presumptive treatment for enteric pathogens, did not improve linear growth from 6 to 12 months of age. This trial was registered at clinicaltrials.gov as NCT03683667. This trial was registered at clinicaltrials.gov as NCT03683667.

An Egg Intervention Improves Ponderal But Not Linear Growth Among Infants 6–12 mo of Age in Rural Bangladesh

BackgroundAnimal source foods are rich in multiple nutrients. Regular egg consumption may improve infant growth in low- and middle-income countries. ObjectivesTo assess the impact of daily egg consumption on linear growth among 6–12-mo olds in rural Bangladesh. MethodsWe conducted a 2 × 4 factorial cluster-randomized controlled trial allocating clusters (n = 566) to treatment for enteric pathogens or placebo and a daily egg, protein supplement, isocaloric supplement, or control. All arms received nutrition education. Here, we compare the effect of the egg intervention versus control on linear growth, a prespecified aim of the trial. Infants were enrolled at 3 mo. We measured length and weight at 6 and 12 mo and visited households weekly to distribute eggs and monitor compliance. We used linear regression models to compare 12-mo mean length, weight, and z-scores for length-for-age (LAZ), weight-for-length, and weight-for-age (WAZ), and log-binomial or robust Poisson regression to compare prevalence of stunting, wasting, and underweight between arms. We used generalized estimating equations to account for clustering and adjusted models for baseline measures of outcomes. ResultsWe enrolled 3051 infants (n = 283 clusters) across arms, with complete 6 and 12 mo anthropometry data from 1228 infants (n = 142 clusters) in the egg arm and 1109 infants (n = 141 clusters) in the control. At baseline, 18.5%, 6.0%, and 16.4% were stunted, wasted, and underweight, respectively. The intervention did not have a statistically significant effect on mean LAZ (β: 0.05, 95% confidence interval [CI]: −0.01, 0.10) or stunting prevalence (β: 0.98, 95% CI: 0.89, 1.13) at 12 mo. Mean weight (β: 0.07 kg, 95% CI: 0.02, 0.11) and WAZ (β: 0.06, 95% CI: 0.02, 0.11) were significantly higher in the egg compared with control arms. ConclusionsProvision of a daily egg for 6 mo to infants in rural Bangladesh improved ponderal but not linear growth. Trial registration numberNCT03683667, https://clinicaltrials.gov/ct2/show/NCT03683667.

The 6-month effect of whole soy and purified isoflavones daidzein on thyroid function-A double-blind, randomized, placebo controlled trial among Chinese equol-producing postmenopausal women.

The aim of this study was to examine the effect of whole soy and purified daidzein on markers of thyroid function in Chinese postmenopausal women who were equol-producers. Total 270 eligible women were randomized to either one of the three isocaloric supplements: 40 g soy flour (whole soy group), 40 g low-fat milk powder +63 mg daidzein (daidzein group) or 40 g low-fat milk powder (placebo) daily for 6months. Serum thyroid markers were tested at baseline and 6months for thyroid stimulating hormone, free triiodothyronine, reverse triiodothyronine and free thyroxine (FT4). There was no significant difference in the 6-month changes of thyroid markers among the three groups. Subgroup analysis among women with lowered thyroid function suggested a modest decrease of FT4. This randomized controlled trial among Chinese equol-producing postmenopausal women indicates the consumption of whole soy and purified daidzein at the provided dosages are safe and have no detrimental effect on thyroid function.

Effects of Bilberry and Oat intake on lipids, inflammation and exercise capacity after Acute Myocardial Infarction (BIOAMI): study protocol for a randomized, double-blind, placebo-controlled trial

BackgroundBilberries from Sweden, rich in polyphenols, have shown cholesterol-lowering effects in small studies, and the cholesterol-lowering properties of oats, with abundant beta-glucans and potentially bioactive phytochemicals, are well established. Both may provide cardiometabolic benefits following acute myocardial infarction (AMI), but large studies of adequate statistical power and appropriate duration are needed to confirm clinically relevant treatment effects. No previous study has evaluated the potential additive or synergistic effects of bilberry combined with oats on cardiometabolic risk factors. Our primary objective is to assess cardioprotective effects of diet supplementation with dried bilberry or with bioprocessed oat bran, with a secondary explorative objective of assessing their combination, compared with a neutral isocaloric reference supplement, initiated within 5 days following percutaneous coronary intervention (PCI) for AMI.MethodsThe effects of Bilberry and Oat intake on lipids, inflammation and exercise capacity after Acute Myocardial Infarction (BIOAMI) trial is a double-blind, randomized, placebo-controlled clinical trial. A total of 900 patients will be randomized post-PCI to one of four dietary intervention arms. After randomization, subjects will receive beverages with bilberry powder (active), beverages with high-fiber bioprocessed oat bran (active), beverages with bilberry and oats combined (active), or reference beverages containing no active bilberry or active oats, for consumption twice daily during a 3-month intervention. The primary endpoint is the difference in LDL cholesterol change between the intervention groups after 3 months. The major secondary endpoint is exercise capacity at 3 months. Other secondary endpoints include plasma concentrations of biochemical markers of inflammation, metabolomics, and gut microbiota composition after 3 months.DiscussionControlling hyperlipidemia and inflammation is critical to preventing new cardiovascular events, but novel pharmacological treatments for these conditions are expensive and associated with negative side effects. If bilberry and/or oat, in addition to standard medical therapy, can lower LDL cholesterol and inflammation more than standard therapy alone, this could be a cost-effective and safe dietary strategy for secondary prevention after AMI.Trial registrationClinicalTrials.gov NCT03620266. Registered on August 8, 2018.

PSV-3 Milk Lactose Concentration Is Altered in Multiparous Sows Supplemented with Sugar from Mid-gestation Through Lactation

Abstract Using swine as a biomedical model, this study examined whether consumption of added protein (red meat) or free sugar (glucose) throughout gestation and lactation will affect milk composition. Multiparous sows were fed an isocaloric supplement in addition to their normal corn-soybean meal base diet starting on day 40 of gestation and throughout lactation: cooked ground beef (BEEF; n = 10); free sugar (SUG; n = 9); a mixture of the BEEF and SUG diet (BEEFSUG; n = 7); or a caloric equivalent of the base diet (CONTROL; n = 8). Milk samples (30 mL) were collected from each sow at days 0, 6, 12, and 18 of lactation by hand-milking teats after intramuscular injection of 1 cc of oxytocin. Milk was frozen at -20 °C until analyzed for percentage of fat, protein, lactose, total solids, other solids, and milk urea nitrogen (MUN). Analyses were conducted using the mixed procedure in SAS. For traits occurring over time, a repeated measures statement was used with sow as the subject and the best fit method based on AICC was chosen. There was a day × treatment interaction (P &amp;lt; 0.01) for fat and lactose. There were no significant treatment effects within a day for fat when evaluating mean separations. While percentages were similar across treatment groups on days 0, 6, and 12 for lactose, SUG sows had decreased lactose on day 18 compared to CONTROL and BEEFSUG sows (P = 0.02 and 0.04), with no other significant differences between treatments. There were no treatment or treatment ×day interactions for protein, MUN, total solids or other solids. There was a main effect of day where protein decreased from day 0 to 12; MUN decreased between days 6 and 18; total solids decreased from day 0 to 6; and other solids increased from day 0 to 6 and further increased on day 12. Consumption of a maternal supplementation may impact the percentage of lactose in milk.

210 Effects of supplementing calcium salts of fatty acids during late gestation on beef cow performance and offspring pre-weaning performance

Abstract Objectives were to investigate the effects of late-gestation supplementation of Ca salts of fatty acids to beef cows on cow performance and offspring pre-weaning growth performance. One hundred and ninety fall-calving, Angus Simmental cows were ranked by BW, BCS, and age. Cows were randomly assigned into 12 endophyte-infected, tall fescue pastures (4 pastures/treatment; 15–16 cows/pasture). Cows were fed an isocaloric supplement with soybean hulls mixed with: whole-shelled corn (CON), 155 g/cow/d of EnerGII (SFA/MUFA), or 40 g/cow/d of Strata 120 g/cow/d of Prequel (PUFA) during the last 83 4.9 d of gestation. Cow BW and BCS were measured at the initial, middle point, and end of supplementation, as well as at breeding and weaning. Milk yield was evaluated by weigh-suckle-weigh technique at 675.0 d postpartum. Steers were weaned at 174 5.0 d of age. Growth performance, milk, and blood parameters were analyzed with MIXED and reproductive data were analyzed with GLIMMIX procedures of SAS. The BW and BCS of the cows were not different (P 0.19) from trial initiation through weaning. Birth BW of the steers was not different (P = 0.62). The AI or overall pregnancy rates were not different (P 0.88). There was no difference (P 0.12) in milk yield or components. However, C15:0 and total n-3 fatty acids in milk from dams supplemented with PUFA was greater (P 0.05) than CON, while SFA/MUFA was intermediate and not different than the others. There was no difference (P = 0.83) for weaning BW of the steers. In conclusion, late-gestation supplementation of Ca salts of PUFA had no effect on cow BW, BCS, or milk yield, but increased total n-3 fatty acids in milk. Neither birth BW nor weaning BW of steers were affected by maternal fatty acid supplementation.

Exploring the metabolic fate of medium-chain triglycerides in healthy individuals using a stable isotope tracer

Medium chain triglyceride (MCT) supplementation is often recommended as treatment for patients with long-chain fatty acid β-oxidation (lcFAO) disorders, since they can be utilized as an energy source without the use of the defective enzyme. However, studies in mice and preterm infants suggest that not all medium-chain fatty acids (MCFA) are oxidized and may undergo elongation to long-chain fatty acids (LCFA). In this single blinded study, we explored the metabolic fates of MCT in healthy individuals using a 13C-labeled MCT tracer. Three healthy males in rest received on two test days a primed continuous infusion of glyceryl tri[1,2,3,4-13C4]-octanoate with either an isocaloric supplementation of 1) exclusively MCT (MCT-only) or 2) a mixture of MCT, proteins and carbohydrates (MCT-mix). Gas chromatography - combustion - isotope ratio mass spectrometry (GC-C-IRMS) was used to determine 13C-enrichment of long-chain fatty acids in plasma and of 13CO2 in exhaled air. When provided as single energy source, an estimated 42% of administered MCT was converted to CO2. In combination with carbohydrates and proteins in the diet, oxidation of MCT was higher (62%). In both diets <1% of 13C-label was incorporated in LCFA in plasma, indicating that administered MCT underwent chain-elongation to LCT. Although the relative MCT oxidation rate was higher when combined with carbohydrates and protein, quantitatively more MCT was oxidized when given an isocaloric meal with solely MCT. As these results were obtained in the resting state opposed to during exercise, it is too early to give a recommendation concerning the use of MCT in lcFAO disorders. The data show that in resting healthy individuals only a very small part of the MCT is traced back as LCFA in plasma, suggesting that MCT treatment does not result in a large LCFA burden, however further research on storage of MCT in tissues is warranted. The study was registered in the Nederlands Trialregister. Protocol ID: Trial NL7417 (NTR7650).

Effect of whole soy and purified daidzein on androgenic hormones in chinese equol-producing post-menopausal women: a six-month randomised, double-blinded and Placebo-Controlled trial

A randomised, double-blind and placebo-controlled trial was performed to examine the effects of whole soy and isoflavone daidzein on serum androgenic hormones in Chinese equol-producing post-menopausal women. A total of 270 eligible women aged 45–70 years were randomised to either one of the three iso-caloric supplements: 40 g soy flour (whole soy group), 40 g low-fat milk powder +63 mg daidzein (daidzein group) or 40 g low-fat milk powder (placebo group) daily for 6 months. Fasting venous samples were tested for serum androstenedione (AD), testosterone (T), prolactin, sex hormone binding globulin and dehydroepiandrosterone sulphate. Intention-to-treat analysis indicated that serum T (p = .022) and AD (p = .05) levels modestly but significantly decreased after 6-month daidzein treatment in comparison with placebo, with a mean difference of –0.057 nmol/L (95%CI: –0.185 to 0.070, p = .018) and –0.118 ng/mL (95%CI: –0.240–0.004, p = .045), respectively. This 6-month trial suggested that purified daidzein may exhibit less androgenic effect.Trial registration: The trial was registered in ClinicalTrials.gov with identifier of NCT01270737. (URL: http://clinicaltrials.gov/ct2/show/NCT01270737.)

Supplement with whey protein hydrolysate in contrast to carbohydrate supports mitochondrial adaptations in trained runners

BackgroundProtein supplementation has been suggested to augment endurance training adaptations by increasing mixed muscle and myofibrillar protein synthesis and lean body mass. However, a potential beneficial effect on mitochondrial adaptations is yet to be clarified.The aim of the present study was to investigate the effect of consuming whey protein hydrolysate before and whey protein hydrolysate plus carbohydrate (PRO-CHO) after each exercise session during a six-week training period compared to similarly timed intake of isocaloric CHO supplements on biomarkers of mitochondrial biogenesis, VO2max and performance in trained runners.MethodsTwenty-four trained runners (VO2max 60.7 ± 3.7 ml O2 kg− 1 min1) completed a six-week block randomized controlled intervention period, consisting of progressive running training. Subjects were randomly assigned to either PRO-CHO or CHO and matched in pairs for gender, age, VO2max, training and performance status. The PRO-CHO group ingested a protein beverage (0.3 g kg− 1) before and protein-carbohydrate beverage (0.3 g protein kg− 1 and 1 g carbohydrate kg− 1) after each exercise session. The CHO group ingested an energy matched carbohydrate beverage. Resting muscle biopsies obtained pre and post intervention were analyzed for mitochondrial specific enzyme activity and mitochondrial protein content. Subjects completed a 6 K time trial (6 K TT) and a VO2max test pre, midway (only 6 K TT) and post intervention.ResultsFollowing six weeks of endurance training Cytochrome C (Cyt C) protein content was significantly higher in the PRO-CHO group compared to the CHO group (p < 0.05), with several other mitochondrial proteins (Succinate dehydrogenase (SDHA), Cytochrome C oxidase (COX-IV), Voltage-dependent anion channel (VDAC), Heat shock protein 60 (HSP60), and Prohibitin (PHB1)) following a similar, but non-significant pattern (p = 0.07–0.14). β-hydroxyacyl-CoA dehydrogenase (HAD) activity was significantly lower after training in the CHO group (p < 0.01), but not in the PRO-CHO group (p = 0.24). VO2max and 6 K TT was significantly improved after training with no significant difference between groups.ConclusionIntake of whey PRO hydrolysate before and whey PRO hydrolysate plus CHO after each exercise session during a six-week endurance training period may augment training effects on specific mitochondrial proteins compared to intake of iso-caloric CHO but does not alter VO2max or 6 K TT performance.Trial registrationclinicaltrials.gov, NCT03561337. Registered 6 June 2018 – Retrospectively registered.

Effect of whole soy and isoflavones daidzein on bone turnover and inflammatory markers: a 6-month double-blind, randomized controlled trial in Chinese postmenopausal women who are equol producers.

Background:Human studies have demonstrated the beneficial effects of soy or isoflavones on bone metabolism. However, conflicting data remain. Equol is the intestinal metabolite of the isoflavone daidzein. The health benefits of soy are more pronounced in equol producers than those not producing equol. This 6-month randomized controlled trial aimed to examine the effect of whole soy (soy flour) and purified daidzein on bone turnover markers (BTMs) in Chinese postmenopausal women who are equol producers.Methods:A total of 270 eligible women were randomized to either one of the three isocaloric supplements as follows: 40 g soy flour (whole soy group), 40 g low-fat milk powder + 63 mg daidzein (daidzein group), or 40 g low-fat milk powder (placebo group) given as a solid beverage daily for 6 months. The following fasting venous samples were collected at the baseline and end of the trial to analyze BTMs: serum cross-linked C-telopeptides of type I collagen, bone-specific alkaline phosphatase, osteocalcin, procollagen type I N-terminal propeptide, and 25(OH)D3. Inflammation-related biomarkers, such as serum interleukin-6, tumor necrosis factor-alpha, C-reactive protein, transferrin, and homocysteine, were also tested to explore potential mechanisms.Results:A total of 253 subjects validly completed the study protocol. Urinary isoflavones suggested a good compliance to the treatments. Intention-to-treat and per-protocol analyses indicated no significant difference in the 6-month or percentage changes in the parameters of bone metabolism and inflammatory markers among the three treatment groups.Conclusions:Whole soy and purified daidzein at provided dosages exhibited no significant effect on the bone metabolism and inflammation levels among Chinese equol-producing postmenopausal women.Trial registration:ClinicalTrials.gov identifier NCT01270737.

PSIV-11 The effect of a high sugar supplement versus a beef supplement during pregnancy on offspring hepatic gene expression in a swine biomedical model

Abstract Our objective was to investigate the effect of a maternal supplemental calorie source, (high protein or high sugar) on offspring developmental programming of metabolic gene expression. Multiparous sows (n = 35) were bred to one of two full-sibling boars. Sows were fed a corn and soybean meal-based diet and randomly assigned to one of four isocaloric supplement treatments: 126 g of the basal diet, (CON, n = 5); 85 g of crystalized sugar (SUGAR, n = 5); 110 g of cooked ground beef (BEEF, n = 6); or a combination of 55 g cooked beef and 43 g of crystalized sugar (B+S, n = 5). Supplements were fed three times daily from d 40 of gestation through d 18 of weaning. A male and female offspring of median weight from each sow were individually housed and phase fed standard production diets. Offspring were harvested at d 160 of age and a liver tissue sample collected. Expression of the following genes was quantified via qPCR with hypoxanthine phosphoribosyltransferase 1 (HPRT1) as the reference gene: pyruvate carboxylase (PC); phosphoenolpyruvate carboxykinase 1 (PCK1); lipase C hepatic type (LIPC); fibroblast growth factor 21 (FGF21); glucose-6 phosphatase catalytic subunit (G6PC); and fructose 1, 6-biphosphate (FBFase). Genes were selected based on their function in hepatic metabolism. Data were analyzed using the GLM procedure of SAS with sex, treatment, and interaction as fixed effects. Sex influenced (P &amp;lt; 0.05) expression of PC and FBFase, with males having greater expression than females (1.38 vs. 0.63 ± 0.14 and 1.15 vs. 0.82 ± 0.09, respectively). However, there was no effect of treatment or sex by treatment interaction on hepatic expression of the genes studied (P &amp;gt; 0.05). Therefore, we conclude that supplemental caloric source during pregnancy does not alter developmental programming of the stated liver metabolic genes in peripubertal offspring.

Short Communication: Effect of dietary protein level on growth and body condition score of male Beetal goats during summer

The effects of feeding various dietary protein levels on the fattening of male Beetal goats were investigated. Twenty-seven bucks (initial bodyweight 35 ± 0.5 kg) between 12 and 16 months old were randomly assigned to one of three treatments The treatments, which were provided as isocaloric supplements to 5 kg fresh sorghum fodder, differed in the amount of crude protein (CP), namely i) 18% CP (LP) ii) 25.5% CP (MP) and iii) 31.6% CP (HP). Feed intake and faecal score were recorded daily. Live bodyweight was recorded every 14 days. Wither height, heart girth, body length and body condition score were recorded monthly. Dry matter intake and average daily gain increased linearly with the level of CP in the diet. The average body condition score, wither height and heart girth were significantly greater in for goats in the HP group compared with those in LP and MP groups. However, dietary protein did not affect body length. Thus, feeding higher levels of CP linearly increased the growth, feed intake and feed efficiency of growing Beetal goats. The mean faecal score was lower for goats in the HP group compared with goats in the LP group.Keywords: average daily gain, body measurements, faecal consistency

Effects of Replacing Supplemental Sucrose with Beef During Mid to Late Gestation on Maternal Health and Fetal Development using a Sow Biomedical Model

ObjectivesThe objectives of this study were to investigate the influence of substituting supplemental sucrose with beef on maternal health and fetal development using a sow biomedical model.Materials and MethodsMultiparous crossbred sows (BW = 222 kg; n = 21; rep = 3) were individually housed from d 30 to 111 (± 0.58) of gestation. From d 30 to 39, a complete sow ration (corn-soybean meal-based, CSM) was fed at 1% of d 30 gestational BW. On d 39, daily dietary ration was adjusted to 1% of d 39 gestational BW which was fed daily at 0700 h from d 40 to 110 (± 0.58). Sows were randomly assigned to 1 of 4 isocaloric supplement treatments; 126 g CSM to serve as a control (CON, n = 5), 110 g cooked ground beef (BEEF, n = 6), 85.5 g sucrose (SUCR, n = 5), or 54.8 g BEEF and 42.7 g SUCR (B+S, n = 5). Dietary supplements were fed daily at 1100, 1500, and 1800 h from d 40 to 110 (± 0.58). Blood was collected via jugular venipuncture from sows on d 29 and 111 (± 0.58). Blood chemistry was immediately analyzed, and serum samples were collected for lipid panel and insulin concentrations. Bodyweights were measured on d 30, 39, 54, 68, 82, 96, and 111 (± 0.58). Tenth rib and last rib SQ fat depth were measured on d 35, 70, and 110 (± 0.58) via ultrasound. Sows were euthanized on d 111 (± 0.58). Reproductive tract (RT), pancreas, kidney, liver, heart, heart fat, lung, semimembranosus and abductor (SM), and semitendinosus (ST) weights were collected and recorded from each sow. Two median weight male and female fetuses were selected from each sow for tissue collections. Fetal tissue collection was the same as sows with the addition of testes and no RT weight. A repeated measures design, with sow as the repeated measure, was modeled using the MIXED procedure of SAS using compound symmetry variance covariance matrix. Sow data fixed effects were replicate, sow, and treatment. Fetal data fixed effects were replicate and fetal weight category. Covariates were determined for each individual trait depending on goodness of fit. A treatment by day interaction was used for sow data while a treatment by sex interaction was used for fetal data. Alpha level was 0.05.ResultsDietary treatment did not influence gestational BW (P ≥ 0.99), SQ fat depth (P ≥ 0.09), blood chemistry (P ≥ 0.21), or serum concentrations (P ≥ 0.07). Dietary treatment did not influence sow tissue weight (P ≥ 0.42). Compared with CON, BEEF fetuses had greater fetal BW (P = 0.01), crown to rump length (P = 0.01), nose to crown length (P &lt; 0.01), heart girth (P = 0.02), and abdominal girth (P = 0.05). Dietary treatment did not influence fetal growth characteristics of median weight male and female fetuses (P ≥ 0.23). Compared with BEEF, SUCR fetuses had heavier liver weights (31.43 ± 2.06 and 40.13 ± 2.09, respectively; P = 0.04). There was a dietary treatment by sex interaction for fetal kidney weight with BEEF males having lighter kidney weights compared with all other interactions (P = 0.03). Dietary treatment did not influence any other fetal tissue weight (P ≥ 0.09).ConclusionBeef and/or sucrose supplementation during mid-to-late gestation has minimal effects on swine maternal health and fetal development. Differences in fetal liver and kidney weights should be examined further. Further research is needed to determine the effect of gestational supplementation on human health and development.

Effects of Protein, Fat and Both Protein and Fat on Glycemic Response of a Meal (P18-094-19)

ObjectivesTo determine if isocaloric supplementation of a high glycemic load breakfast with protein, fat, or a combination of the two attenuates the glycemic response in non-diabetic subjects.Elevated blood glucose (BG) levels from a diet high in refined carbohydrates, even in the absence of diabetes, may increase the risk for chronic diseases including cardiovascular disease, type 2 diabetes, and obesity. Addition of protein and fat have shown inconsistently delay the glycemic response to a carbohydrate load. MethodsThirteen healthy adults, age 24.7 ± 4y, BMI 25.1 ± 4.5 completed four trials for this crossover study. The participants had to be non-smoker, between 18–30 years of age, with no known allergy to any components of the control or treatment meals. Exclusion criteria included weight under 110 Ib, pregnancy or breastfeeding, and any medical condition or medication that affects BG considered. The participants received the control and all treatments in different sessions at random. The subjects consumed 2 slices of white bread and 250 mL of apple juice (60 g carbohydrate) alone (control), or with an added protein (100 kcal egg white), fat (100 kcal butter), or protein + fat (50 kcal egg white and 50 kcal butter) within 15 min. Fasting BG was measured and BG was measured at 15, 30, 60, 90, and 120 min, after the meals was consumed. A One way Repeated measures ANOVA test used to compare the BG spike, timing and iAUC in the BG among the four trials, Tukey’s post-hoc test was performed to indicate if there was a difference in the spike, timing and iAUC. ResultsThe results indicated the spike in BG (peak minus baseline) was significantly lower with added fat group compared to added protein group (69.0 ± 15.4 vs 46.9 ± 13.0, respectively, P < 0.05). At 15 and 30 min, BG was higher in the added protein group (142.77 + 13.73 and 150.38 + 21.82) compared to the added fat group (126.35 + 9.97 and 126.5 + 17.23). However; the spike was not significantly different between the control and the treatments. There was no significant difference in iAUC among the control and the treatments. ConclusionsIn this study added protein, fat, or protein + fat in a 100 kcal amount did not influence the spike in BG in response to a high refined carbohydrate meal. The fat delays the spike in the BG compared to the protein and protein + fat groups. Funding SourcesUniversity of the Incarnate Word.

Longissimus Muscle Composition and Palatability of Grazing Steers Supplemented with Corn Oil or Corn Grain

Twenty-eight Angus (289 ± 3.8 kg) steers were used to evaluate the effect of isocaloric supplementation of 2 different energy sources to steers grazing tall fescue pastures for 197 d on longissimus muscle fatty acid profile, shear force, tenderness and color. Steers were supplemented with either corn grain (PC) or soybean hulls plus corn oil (PO). A negative control, pasture only (PA), and positive control, high-concentrate control diets (CONC) were also included in the study. Total trans-11 vaccenic acid (TVA) and cis-9, trans-11 CLA content per serving were similar with PA, PC and CONC and greatest with PO (P &lt; 0.001). Muscle total fatty acids, myristic and palmitic contents per serving were similar with PC, PO, and PA and greatest with CONC (P &lt; 0.001). Muscle PUFA n-6:n-3 ratio was greater with PC than with PA and lower with PC than with CONC, but it was greatest with PO (P &lt; 0.001). Shear force was lower (P = 0.046) with CONC than with PA and PC; beef from PO did not differ from any of the other treatments. Sensory panel scores for overall tenderness (P &lt; 0.001) were greatest with CONC, greater with PO than with PC, and similar with PA than with PO and PC. Muscle lightness was similar for PO and PC, greater with PO and PC than with PA and lower with PO and PC than with CONC (P &lt; 0.001). Treatment by time postmortem interaction was significant for muscle temperature (P &lt; 0.001), but not for muscle pH (P = 0.79). Temperature decline was fastest with PA and slowest with CONC. Postmortem muscle pH was greater with PA, PC, and PO than with CONC (P = 0.011). Overall, fatty acid profile with PC was closer to the fatty acid profile with PA than that with PO or CONC. Finishing systems altered fat deposition, which impacted chilling rate, muscle color and palatability.

PSII-2 Effects of Replacing Supplemental Sucrose with Beef on Maternal Health and Fetal Growth and Development Using a Sow Biomedical Model.

ObjectivesA diet high in sucrose is reflective of a Western diet that the average American consumes. When consuming a Western diet, individuals consume roughly 20 teaspoons of sucrose per day. The Western diet has been previously linked to metabolic disorders such as diabetes and obesity, which may be prevented by replacing dietary sucrose with a healthy protein alternative like beef. The objectives of this study were to investigate the impact of substituting dietary sucrose with beef supplementation on maternal health and fetal development utilizing the sow as a biomedical model. Materials and MethodsMultiparous pregnant sows (Landrace × Yorkshire; BW = 222 kg; n = 21) were fed a complete sow gestation diet (corn-soybean meal-based, CSM; NRC 2017) at 1 percent of BW on sow d 30 of gestation at 700 h daily from d 30 to 39 of gestation. Sows were then fed CSM at 1 percent of BW on d 39 of gestation at 700 h daily from d 40 to 110 (± 0.58) of gestation. Sows were randomly assigned to 1 of 4 isocaloric dietary supplements consisting of 126 g CSM (CON, n = 5); 110 g cooked ground beef (CGB; BEEF, n = 6); 54.8 g CGB and 42.7 g sucrose (B+S, n = 5); or 85.5 g sucrose (SUCROSE, n = 5). Dietary supplements were fed 3 times daily from d 40 to 110 (± 0.58) of gestation. Sows were housed in gestation crates from d 30 to 111 (± 0.58) of gestation and euthanized on d 111 (± 0.58) of gestation. Blood was collected via jugular venipuncture from sows on d 29 and 111 (± 0.58) of gestation. Blood chemistry was immediately analyzed using iSTAT point-of-care device. Bodyweights were measured on d 30, 39, 54, 68, 82, 96, and 111 (± 0.58) of gestation. Tenth rib and last rib fat depth were measured on d 35, 70, and 110 (± 0.58) of gestation utilizing an ALOKA SSD-500V ultrasound. Fetal growth measurements were recorded for all fetuses. Two median weight male and female fetuses were selected from each sow for tissue collections which included pancreas, kidney, liver, heart, heart fat, lung, empty body weight, semimembranosus, and semitendinosus weights. A repeated measures design, using sow as the repeated measure, was modeled using the MIXED procedure of SAS (SAS Inst. Inc., Car, NC) using compound symmetry variance covariance matrix. Alpha level was 0.05. ResultsDietary treatment did not influence blood metabolites of sows on d 29 or 111 of gestation (P = 0.09 and P = 0.20, respectively). Sow weight throughout gestation was not influenced by dietary treatment (P = 0.74). Dietary treatment did not influence tenth rib or last rib fat depth on d 35, 70, or 110 of gestation (P = 0.27). Compared to CON and SUCROSE, fetuses from sows supplemented with BEEF had greater nose to crown lengths (6.03 ± 0.29, 6.02 ± 0.28, and 7.33 ± 0.36 cm, respectively; P = 0.04). Compared to CON, fetuses from sows supplemented with sucrose had respectively greater BW (1296 ± 73.6 vs. 1556 ± 68.8 g; P = 0.02); heart girths (22.90 ± 0.39 vs. 24.13 ± 0.39 cm; P = 0.03), and liver weights (36.48 ± 2.19 vs. 43.44 ± 2.05 g; P = 0.04). Dietary treatment did not influence other fetal characteristics or organ weights (P ≥ 0.05). ConclusionBeef supplementation during mid to late pregnancy had minimal effects on maternal blood metabolites, bodyweight, or backfat depth or fetal growth. The increase in fetal weight due to sucrose supplementation should be further explored.

Protein supplements after weight loss do not improve weight maintenance compared with recommended dietary protein intake despite beneficial effects on appetite sensation and energy expenditure: a randomized, controlled, double-blinded trial

Background: High-protein diets increase weight loss (WL) during energy restriction; therefore, it has been suggested that additional protein intake may improve weight maintenance (WM) after WL.Objective: We investigated the effect of protein supplements from either whey with or without calcium or soy on WM success after WL compared with that of a control.Design: In a randomized, controlled, double-blinded trial, 220 participants aged 18-60 y with body mass index (in kg/m2) from 27.6 to 40.4 were included. The study was initiated with an 8-wk WL period followed by a 24-wk WM period. During WM, participants consumed the following isocaloric supplements (45-48 g/d): whey and calcium (whey+), whey, soy, or maltodextrin (control). Data were collected at baseline, before WM, and after WM (weeks 0, 8, and 32, respectively) and included body composition, blood biochemistry, and blood pressure. Meal tests were performed to investigate diet-induced-thermogenesis (DIT) and appetite sensation. Compliance was tested by 24-h urinary nitrogen excretion.Results: A total of 151 participants completed the WM period. The control and 3 protein supplements did not result in different mean ± SD weight regains (whey+: 2.19 ± 4.6 kg; whey: 2.01 ± 4.6 kg; soy: 1.76 ± 4.7 kg; and control: 2.23 ± 3.8 kg; P = 0.96), fat mass regains (whey+: 0.46 ± 4.5 kg; whey: 0.11 ± 4.1 kg; soy: 0.15 ± 4.1 kg; and control: 0.54 ± 3.3 kg; P = 0.96), or improvements in lean body mass (whey+: 1.87 ± 1.7 kg; whey: 1.94 ± 1.3 kg; soy: 1.58 ± 1.4 kg; and control: 1.74 ± 1.4 kg; P = 0.50) during WM. Changes in blood pressure and blood biochemistry were not different between groups. Compared with the control, protein supplementation resulted in higher DIT (∼30 kJ/2.5 h) and resting energy expenditure (243 kJ/d) and an anorexigenic appetite-sensation profile.Conclusion: Protein supplementation does not result in improved WM success, or blood biochemistry after WL compared with the effects of normal dietary protein intake (0.8-1.0 g · kg-1 · d-1). This trial was registered at clinicaltrials.gov as NCT01561131.

The Efficacy of a Pre-Workout Vegan Supplement on High-Intensity Cycling Performance in Healthy College-Aged Males

ABSTRACTThere is a limited supply of sport nutrition supplements currently available for vegan or vegetarian athletes. In addition, the efficacy of a vegan or vegetarian pre-workout supplement that does not contain any processed ingredients or stimulants is currently unknown. The purpose of the current study was to examine the effects of an unprocessed vegan pre-workout supplement on high-intensity cycling performance. Participants completed three separate cycling trials following the consumption of a vegan pre-workout supplement, an isocaloric processed supplement, or a zero-calorie placebo supplement. Each supplement was consumed 30 minutes prior to each trial, and each cycling trial was separated by a minimum of 72 hours. Supplements were administered using a randomized, double-blind cross-over design. Each cycling trial was performed at a workload equal to 80% VO2peak until exhaustion. The average time in seconds (s) until exhaustion values for the vegan, isocaloric, and zero-calorie supplements were 482 ± 163, 480 ± 157, and 496 ± 238, respectively. Consumption of the vegan supplement did not significantly improve performance compared to an isocaloric and zero-calorie supplement (F = 0.12, p =.89). The results of this study indicate that individuals who choose a vegan pre-workout supplement (over an isocaloric or zero-calorie product) will not experience any acute decrements or ergogenic benefits in cycling performance. Although the present study does not support performance benefits of the tested vegan pre-workout supplement before cycling, additional research examining various exercise intensities and modalities is warranted.

Quantity and source of dietary protein influence metabolite production by gut microbiota and rectal mucosa gene expression: a randomized, parallel, double-blind trial in overweight humans

Background: Although high-protein diets (HPDs) are frequently consumed for body-weight control, little is known about the consequences for gut microbiota composition and metabolic activity and for large intestine mucosal homeostasis. Moreover, the effects of HPDs according to the source of protein need to be considered in this context.Objective: The objective of this study was to evaluate the effects of the quantity and source of dietary protein on microbiota composition, bacterial metabolite production, and consequences for the large intestinal mucosa in humans.Design: A randomized, double-blind, parallel-design trial was conducted in 38 overweight individuals who received a 3-wk isocaloric supplementation with casein, soy protein, or maltodextrin as a control. Fecal and rectal biopsy-associated microbiota composition was analyzed by 16S ribosomal DNA sequencing. Fecal, urinary, and plasma metabolomes were assessed by 1H-nuclear magnetic resonance. Mucosal transcriptome in rectal biopsies was determined with the use of microarrays.Results: HPDs did not alter the microbiota composition, but induced a shift in bacterial metabolism toward amino acid degradation with different metabolite profiles according to the protein source. Correlation analysis identified new potential bacterial taxa involved in amino acid degradation. Fecal water cytotoxicity was not modified by HPDs, but was associated with a specific microbiota and bacterial metabolite profile. Casein and soy protein HPDs did not induce inflammation, but differentially modified the expression of genes playing key roles in homeostatic processes in rectal mucosa, such as cell cycle or cell death.Conclusions: This human intervention study shows that the quantity and source of dietary proteins act as regulators of gut microbiota metabolite production and host gene expression in the rectal mucosa, raising new questions on the impact of HPDs on the large intestine mucosa homeostasis. This trial was registered at clinicaltrials.gov as NCT02351297.

ELEVEN WEEKS OF STRENGTH TRAINING DECREASED THE EXPRESSION OF IMMUNE-RELATED GENES IN OLDER SUBJECTS INDEPENDENT OF PROTEIN SUPPLEMENT TYPE; A RANDOMIZED CONTROLLED TRIAL

Objective: To investigate the effects of eleven weeks of strength training combined with two isocaloric protein supplements on mRNA expression levels in skeletal muscle and peripheral blood mononuclear blood cells (PBMCs). Design: A double blind randomized controlled study. Setting: The Norwegian School of Sports Sciences, Norway. Participants: Untrained, but otherwise healthy, men and women (n=20, ≥ 70 yrs). Intervention: Participants were randomly allocated to receive either milk protein or a native whey protein supplement (20 g protein, morning and afternoon) combined with a standardized strength training protocol (6-10 RM, 1-3 sets, 3 times/week) for eleven weeks. Measurements: The mRNA expression levels of immune-related genes were measured before and after the intervention period, using RT-qPCR. Cytokines were measured using ELISA. Results: PBMC mRNA expression of interleukin (IL) 6, IL8, chemokine (C-C motif) ligand (CCL) 3, and nuclear receptor subfamily (NR) 1 group H (H) member 3 decreased significantly after the intervention period, whereas the mRNA expression of toll-like receptor (TLR2) increased. In skeletal muscle, the mRNA expression of peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PPARGC1A) and PPARGC1B decreased significantly, whereas the mRNA expression of CCL2, CCL5, TLR2, TLR4 and hypoxia inducible factor 1 alpha subunit (HIF1A) increased significantly after the intervention. We found no significant differences in circulating C-reactive protein and IL6 after the intervention period. The consumption of whey and milk proteins had similar effects on mRNA expression levels after strength training in skeletal muscle as well as PBMCs. Conclusion: Eleven weeks of strength training and protein supplementation reduced the PBMC expression levels of genes involved in the immune system as well as in metabolism, underlining the close interaction between these processes. The upregulation of other immune-related genes observed in PBMCs as well as in skeletal muscle needs further investigations, but may be related to protein supplementation and training adaptations. Different protein supplementation (milk or native whey) did not differentially modulate mRNA expression after the intervention period.