A pot experiment was conducted during two successive seasons (2016/2017 and 2017/2018) at the greenhouse of the Ornamental Dept., El-Kassasin Res. Station, Ismailia Governorate, Egypt. This study was carried out to investigate the effect of foliar spraying with some growth regulators (gibberellic acid (GA3) at 100 and 200 ppm, kinetin (Kin) at 100 and 200 ppm beside the control treatment) and a microelements mixture at (0, 1, 2 and 3 g/l) on vegetative growth, root system and quality of peace lily(Spathiphyllum wallisii Regel) plant. Results showed that the interaction treatment between spraying peace lily plants with Kin at 100 ppm in addition to the microelements mixture at 2 g/l gave the highest values regarding number of leaves/plant, stem diameter and leaf area, number of roots/plant in both seasons, fresh weight of roots in the 2nd season and dry weight of root, fresh and dry weight of shoots, number of both shoots and flowers/plant in both seasons. The interaction treatment between spraying plants with GA3 at 200 ppm and 2 g/l microelements mixture gave the tallest plants in both seasons. However, the interaction treatment between Kin at 100 ppm and spraying plants with 3 g/l microelements mixture was the best interaction treatment for enhancing the concentration of total chlorophyll and total carbohydrates in shoots, in both seasons. Generally, the best interaction treatments for increasing growth, flowering and biochemical constituents was obtained with Kin at 100 ppm and 2 g/l microelements.