Blue Light Irradiation Research Articles

Engineering a Dual-Functionalized PolyHIPE Resin for Photobiocatalytic Flow Chemistry.

The use of a dual resin for photobiocatalysis, encompassing both a photocatalyst and an immobilized enzyme, brings several challenges, including effective immobilization, maintaining photocatalyst and enzyme activity and ensuring sufficient light penetration. However, the benefits, such as integrated processes, reusability, easier product separation, and potential for scalability, can outweigh these challenges, making dual resin systems promising for efficient and sustainable photobiocatalytic applications. In this study, we employed a photosensitizer-containing porous emulsion-templated polymer as a functional support that is used to covalently anchor a chloroperoxidase from Curvularia inaequalis (CiVCPO). We demonstrate the versatility of this heterogeneous photobiocatalytic material, which enables the bromination of four aromatic substrates, including rutin-a natural occurring flavonol-under blue light (456 nm) irradiation and continuous flow conditions.

Engineering a Dual‐Functionalized PolyHIPE Resin for Photobiocatalytic Flow Chemistry

AbstractThe use of a dual resin for photobiocatalysis, encompassing both a photocatalyst and an immobilized enzyme, brings several challenges, including effective immobilization, maintaining photocatalyst and enzyme activity and ensuring sufficient light penetration. However, the benefits, such as integrated processes, reusability, easier product separation, and potential for scalability, can outweigh these challenges, making dual resin systems promising for efficient and sustainable photobiocatalytic applications. In this study, we employed a photosensitizer‐containing porous emulsion‐templated polymer as a functional support that is used to covalently anchor a chloroperoxidase from Curvularia inaequalis (CiVCPO). We demonstrate the versatility of this heterogeneous photobiocatalytic material, which enables the bromination of four aromatic substrates, including rutin—a natural occurring flavonol—under blue light (456 nm) irradiation and continuous flow conditions.

Rational Design of TaON/Potassium Poly(Heptazine Imide) Heterostructure for Multifunctional Environmental Remediation

AbstractTantalum oxynitride (TaON) has recently attracted much attention due to its excellent performance as a semiconductor. However, its shortage in separation of photogenerated charges hinders the wide application, which, fortunately, can be made up by forming heterojunctions with other photocatalysts. In this study, the rational design of an inorganic‐organic TaON/potassium poly (heptazine imide) (KPHI) heterojunction is reported to separate the photo charges on the two sides of this dyadic structure. The incorporation of 20 wt.% TaON into KPHI resulted not only in H2 generation but also in a wider set of environmental remediation applications under visible light, including Cr(VI) reduction and degradation of otherwise resilient antibiotics. 20TaON/KPHI effectively reduced 17.5 ppm Cr(VI) solution in 35 min under low‐power blue light irradiation with a rate constant which is ≈6 and 30 times higher than that of pristine KPHI and TaON, respectively. Moreover, the composite demonstrated a 2‐ and 40‐fold increase in H2 evolution rate in comparison to pure KPHI and TaON, respectively. This remarkable enhancement in photocatalytic activity is ascribed to the efficient electron‐hole separation and formation of an effective Z‐scheme heterojunction. This work offers a new paradigm for designing efficient photocatalytic systems for environmental applications under low‐power LED and natural sunlight.

Advancing Photodynamic Antimicrobial Strategy: Sustainable Fabrication of Novel Lauryl Gallate-Chitosan Hydrophobic Films with Rapid Bacterial Capture and Biofilms Elimination Capabilities for Promoting Wound Healing.

Bioinspired photoactive composites, in terms of photodynamic inactivation, cost-effectiveness, and biosafety, are promising alternatives to antibiotics for combating bacterial infections while avoiding antibacterial resistance. However, the weak bacterial membrane affinity of the photoactive substrate and the lack of synergistic antibacterial effect remain crucial shortcomings for their antibacterial applications. Herein, we developed a hydrophobic film from food antioxidant lauryl gallate covalently functionalized chitosan (LG-g-CS conjugates) through a green radical-induced grafting reaction that utilizes synergistic bacteria capture, contact-killing, and photodynamic inactivation activities to achieve enhanced bactericidal and biofilm elimination capabilities. Besides, the grafting reaction mechanism between LG and CS in the ascorbic acid (AA)/H2O2 redox system was further proposed. The LG-g-CS films feature hydrophobic side chains and photoactive phenolic hydroxyl groups, facilitating dual bactericidal activities through bacteria capture and contact-killing via strong hydrophobic and electrostatic interactions with bacterial membranes as well as blue light (BL)-driven photodynamic bacterial eradication through the enhanced generation of reactive oxygen species. As a result, the LG-g-CS films efficiently capture and immobilize bacteria and exhibit excellent photodynamic antibacterial activity against model bacteria (Escherichia coli and Staphylococcus aureus) and their biofilms under BL irradiation. Moreover, LG-g-CS films could significantly promote the healing process of S. aureus-infected wounds. This research demonstrates a new strategy for designing and fabricating sustainable bactericidal and biofilm-removing materials with a high bacterial membrane affinity and photodynamic activity.

Design of a millifluidic device for the evaluation of blue-light disinfection on Pseudomonas aeruginosa PA01 biofilm

SignificanceFor the first time to our knowledge, a millifluidic device has been designed to observe the effects of blue-light irradiation in Pseudomonas aeruginosa PA01 bacterial biofilm. ApproachA bacterial biofilm is formed under controlled flow of nutrients in microfabricated channels settled on a microscope stage. The setup is devised to apply defined irradiation and evaluate the photo-killing efficiency in situ using video-microscopy and fluorescent probes. ResultsWe investigated bacterial survival after delivering a precise, controlled and spatially defined blue light dose upon growing biofilm. We demonstrate in this report that the combined use of constitutive GFP and propidium iodide in a millifluidic setup enables to evidence light-induced mortality at the single cell and community level. ConclusionsReal-time monitoring of a bacterial biofilm growing in a millifluidic device after irradiation opens an avenue for a better understanding of the biofilm community response to blue-light, permitting researchers to approach photokilling efficiency of biofilm as a dynamic process.

Antiviral and synergistic effects of photo-energy with acyclovir on herpes simplex virus type 1 infection

This experimental study aimed to evaluate the antiviral and synergistic effects of photoenergy irradiation on human herpes simplex virus type I (HSV-1) infection. We assessed viral replication, plaque formation, and relevant viral gene expression to examine the antiviral and synergistic effects of blue light (BL) with acyclovir treatment. Our results showed that daily BL (10 J/cm2) irradiation inhibited plaque-forming ability and decreased viral copy numbers in HSV-1-infected monkey kidney epithelial Vero cells and primary human oral keratinocyte (HOK) cells. Combined treatment with the antiviral agent acyclovir and BL irradiation increased anti-viral activity, reducing viral titers and copy numbers. In particular, accumulated BL irradiation suppressed characteristic viral genes including UL19 and US6, and viral DNA replication-essential genes including UL9, UL30, UL42, and UL52 in HOK cells. Our results suggest that BL irradiation has anti-viral and synergistic properties, making it a promising therapeutic candidate for suppressing viral infections in clinical trials.

A supramolecular photosensitizer based on triphenylamine and pyrazine with aggregation-induced emission properties for high-efficiency photooxidation reactions

Recently, there has been a great interest in the study of photocatalysts (PCs) and photosensitizers (PSs) in the field of organic photocatalysis. In the present study, a pure organic thermally activated delayed fluorescence (TADF) molecule 4,4′-(12-(pyridin-4-yl)dibenzo[f,h]pyrido[2,3-b]quinoxaline-3,6-diyl)bis(N,N-diphenylaniline) (DPQ-TPA) was designed and synthesized, which not only have excellent TADF property and small energy splitting (ΔEST), but also can self-assembly in water to form cross-linked nanoparticles with exceptional aggregation-induced emission (AIE) characteristics. DPQ-TPA exhibits excellent remarkable selectivity and notably enhances the production capacity of reactive oxygen species (ROS), particularly 1O2, which was employed as a highly effective photocatalyst in the photooxidation reaction of phosphine and hydroazobenzenes under blue light irradiation with high yields up to 94% and 91%, respectively. This work expands the potential application of (donor-acceptor) D-A type AIE-TADF molecules in photocatalytic organic transformations through supramolecular self-assembly.

Porphyrin-conjugated lignin p-n heterojunction as an effective and biocompatible photosensitizer for antibacterial applications

This work explores the potential of porphyrin-grafted lignin nanoparticles (TCPP-g-AL-NPs) as a novel, effective, and biocompatible photosensitizer for antibacterial applications. Lignin acted as a sustainable framework, enhancing water-compatibility and single oxygen (1O2) generation of porphyrin. The mechanism study revealed the successful formation of a p-n heterojunction between lignin and porphyrin, which promoted the charge separation and the photocatalytic process of O2 to 1O2. In vitro antibacterial assessments demonstrated the efficacy of TCPP-g-AL-NPs in eradicating both Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria under blue LED light irradiation. The observed lower antibacterial effectiveness against E. coli compared to S. aureus could be attributed to the thicker cell membrane of Gram-negative bacteria. Additionally, TCPP-g-AL-NPs exhibited no cytotoxicity towards mouse fibroblast cells (L929). This study presents a straightforward and promising approach for developing lignin-based photosensitizers for photodynamic therapy.

Acridine-Isoxazole and Acridine-Azirine Hybrids: Synthesis, Photochemical Transformations in the UV/Visible Radiation Boundary Region, and Anticancer Activity.

Easy-to-handle N-hydroxyacridinecarbimidoyl chloride hydrochlorides were synthesized as convenient nitrile oxide precursors in the preparation of 3-(acridin-9/2-yl)isoxazole derivatives via 1,3-dipolar cycloaddition with terminal alkynes, 1,1-dichloroethene, and acrylonitrile. Azirines with an acridin-9/2-yl substituent attached directly or via the 1,2,3-triazole linker to the azirine C2 were also synthesized. The three-membered rings of the acridine-azirine hybrids were found to be resistant to irradiation in the UV/visible boundary region, despite their long-wave absorption at 320-420 nm, indicating that the acridine moiety cannot be used as an antenna to transfer light energy to generate nitrile ylides from azirines for photoclick cycloaddition. The acridine-isoxazole hybrids linked at the C9-C3 or C2-C3 atoms under blue light irradiation underwent the addition of such hydrogen donor solvents, such as, toluene, o-xylene, mesitylene, 4-chlorotoluene, THF, 1,4-dioxane, or methyl tert-butyl ether (MTBE), to the acridine system to give the corresponding 9-substituted acridanes in good yields. The synthesized acridine-azirine, acridine-isoxazole, and acridane-isoxazole hybrids exhibited cytotoxicity toward both all tested cancer cell lines (HCT 116, MCF7, and A704) and normal cells (WI-26 VA4).

Photochemical Synthesis of Acyl Fluorides Using Copper-Catalyzed Fluorocarbonylation of Alkyl Iodides.

Acyl fluorides are important reagents due to their unique balance between reactivity and stability. Here, we report a copper-catalyzed carbonylative coupling strategy for synthesizing acyl fluorides under photoirradiation. Alkyl iodides were transformed in high yields into acyl fluorides by using a commercially available copper precatalyst (CuBr·SMe2) and a readily available fluoride salt (KF) at ambient temperature and mild CO pressure (6 atm) under blue light irradiation.

C-H, N-H, and O-H Bond Activations to Prepare Phosphorescent Hydride-Iridium(III)-Phosphine Emitters with Photocatalytic Achievement in C-C Coupling Reactions.

Complex IrH5(PiPr3)2 (1) activates two different σ-bonds of 3-phenoxy-1-phenylisoquinoline, 2-(1H-benzimidazol-2-yl)-6-phenylpyridine, 2-(1H-indol-2-yl)-6-phenylpyridine, 2-(2-hydroxyphenyl)-6-phenylpyridine, N-(2-hydroxyphenyl)-N'-phenylimidazolylidene, and 1,3-di(2-pyridyl)-4,6-dimethylbenzene to give IrH{κ3-C,N,C-[C6H4-isoqui-O-C6H4]}(PiPr3)2 (2), IrH{κ3-N,N,C-[NBzim-py-C6H4]}(PiPr3)2 (3), IrH{κ3-N,N,C-[Ind-py-C6H4]}(PiPr3)2 (4), IrH{κ3-C,N,O-[C6H4-py-C6H4O]}(PiPr3)2 (5), IrH{κ3-C,C,O-[C6H4-Im-C6H4O]}(PiPr3)2 (6), and IrH{κ3-N,C,C-[py-C6HMe2-C5H3N]}(PiPr3)2 (7), respectively. The activations are sequential, with the second generally being the slowest. Accordingly, dihydride intermediates IrH2{κ2-C,N-[C6H4-isoqui-O-C6H5]}(PiPr3)2 (2d), IrH2{κ2-N,N-[NBzim-py-C6H5]}(PiPr3)2 (3d), IrH2{κ2-N,N-[Ind-py-C6H5]}(PiPr3)2 (4d), and IrH2{κ2-N,C-[py-C6HMe2-py]}(PiPr3)2 (7d) were characterized spectroscopically. Complexes 3 and 5 are green phosphorescent emitters upon photoexcitation, exhibiting good absorption over a wide range of wavelengths, emission quantum yields about 0.70 in solution, long enough lifetimes (10-17 μs), and reversible electrochemical behavior. In agreement with these features, complex 3 promotes the photocatalytic α-amino C(sp3)-H arylation of N,N-dimethylaniline and N-phenylpiperidine with 1,4-dicyanobenzene and 4-cyanopyridine under blue LED light irradiation. The C-C coupling products are isolated in high yields with only 2 mol % of photocatalyst after 24 h.

Integrated transcriptome and metabolome analysis reveals anthocyanin biosynthesis mechanisms in pepper (Capsicum annuum L.) leaves under continuous blue light irradiation

BackgroundDifferent metabolic compounds give pepper leaves and fruits their diverse colors. Anthocyanin accumulation is the main cause of the purple color of pepper leaves. The light environment is a critical factor affecting anthocyanin biosynthesis. It is essential that we understand how to use light to regulate anthocyanin biosynthesis in plants.ResultPepper leaves were significantly blue–purple only in continuous blue light or white light (with a blue light component) irradiation treatments, and the anthocyanin content of pepper leaves increased significantly after continuous blue light irradiation. This green-to-purple phenotype change in pepper leaves was due to the expression of different genes. We found that the anthocyanin synthesis precursor-related genes PAL and 4CL, as well as the structural genes F3H, DFR, ANS, BZ1, and F3’5’H in the anthocyanin synthesis pathway, had high expression under continuous blue light irradiation. Similarly, the expression of transcription factors MYB1R1-like, MYB48, MYB4-like isoform X1, bHLH143-like, and bHLH92-like isoform X3, and circadian rhythm-related genes LHY and COP1, were significantly increased after continuous blue light irradiation. A correlation network analysis revealed that these transcription factors and circadian rhythm-related genes were positively correlated with structural genes in the anthocyanin synthesis pathway. Metabolomic analysis showed that delphinidin-3-O-glucoside and delphinidin-3-O-rutinoside were significantly higher under continuous blue light irradiation relative to other light treatments. We selected 12 genes involved in anthocyanin synthesis in pepper leaves for qRT-PCR analysis, and the accuracy of the RNA-seq results was confirmed.ConclusionsIn this study, we found that blue light and 24-hour irradiation together induced the expression of key genes and the accumulation of metabolites in the anthocyanin synthesis pathway, thus promoting anthocyanin biosynthesis in pepper leaves. These results provide a basis for future study of the mechanisms of light quality and photoperiod in anthocyanin synthesis and metabolism, and our study may serve as a valuable reference for screening light ratios that regulate anthocyanin biosynthesis in plants.

Highly efficient one-component benzylidene cyclopentanone initiators for visible light polymerization

Visible light polymerization is a development trend in the field of photocuring. However, most of the reported visible light photoinitiators (PIs) are two-component systems that display limited initiation efficiency due to diffusion restriction and back electron transfer effect. In this study, two one-component visible light PIs (BDNPG-1 and BDNPG-2) are synthesized through combining N-phenylglycine (NPG) moiety with cyclopentanone moiety together. Upon irradiation of blue or green light, they can undergo intermolecular electron transfer followed by proton transfer plus a decarboxylation reaction to produce carbon dioxide and aminoalkyl radicals. This decarboxylation reaction is irreversible so that the intermolecular back electron transfer can be effectively blocked, resulting in a significantly improved photoinitiation efficiency of BDNPG-1 and BDNPG-2 compared with their prototype PI (BDEA) containing no NPG group and the two-component system of BDEA+NPG. Furthermore, both of them have strong two-photon absorption capability and show excellent performance in two-photon polymerization.

Red Light-Blue Light Chromoselective C(sp2)-X Bond Activation by Organic Helicenium-Based Photocatalysis.

Chromoselective bond activation has been achieved in organic helicenium (nPr-DMQA+)-based photoredox catalysis. Consequently, control over chromoselective C(sp2)-X bond activation in multihalogenated aromatics has been demonstrated. nPr-DMQA+ can only initiate the halogen atom transfer (XAT) pathway under red light irradiation to activate low-energy-accessible C(sp2)-I bonds. In contrast, blue light irradiation initiates consecutive photoinduced electron transfer (conPET) to activate more challenging C(sp2)-Br bonds. Comparative reaction outcomes have been demonstrated in the α-arylation of cyclic ketones with red and blue lights. Furthermore, red-light-mediated selective C(sp2)-I bonds have been activated in iodobromoarenes to keep the bromo functional handle untouched. Finally, the strength of the chromoselective catalysis has been highlighted with two-fold functionalization using both photo-to-transition metal and photo-to-photocatalyzed transformations.

Immunoactivation by Cutaneous Blue Light Irradiation Inhibits Remote Tumor Growth and Metastasis.

An improved innate immunity will respond quickly to pathogens and initiate efficient adaptive immune responses. However, up to now, there have been limited clinical ways for effective and rapid consolidation of innate immunity. Here, we report that cutaneous irradiation with blue light of 450 nm rapidly stimulates the innate immunity through cell endogenous reactive oxygen species (ROS) regulation in a noninvasive way. The iron porphyrin-containing proteins, mitochondrial cytochrome c (Cyt-c), and cytochrome p450 (CYP450) can be mobilized by blue light, which boosts electron transport and ROS production in epidermal and dermal tissues. As a messenger of innate immune activation, the increased level of ROS activates the NF-κB signaling pathway and promotes the secretion of immunomodulatory cytokines in skin. Initiated from skin, a regulatory network composed of cytokines and immune cells is established through the circulation system for innate immune activation. The innate immunity activated by whole-body blue light irradiation inhibits tumor growth and metastasis by increasing the infiltration of antitumor neutrophils and tumor-associated macrophages. Our results elucidate the remote immune modulation mechanism of blue light and provide a clinically applicable way for innate immunity activation.

Ce3+-Activated SrLu2Al3ScSiO12 Cyan-Green-Emitting Garnet-Structured Inorganic Phosphor Materials toward Application in Blue-Chip-Based Phosphor-Converted Solid-State White Lighting.

Phosphor-converted white-light-emitting diodes (WLEDs) with superhigh color rendering index (CRI) are the ongoing pursuit of next-generation solid-state lighting. One of the most important challenges is the limited improvement in CRI on account of the absence of a cyan component in the typical commercial combination. Here, a bright broad-band cyan-green-emitting phosphor with cubic garnet structure, SrLu2Al3ScSiO12:Ce3+ (SLASSO:Ce3+), was successfully reported, which can compensate for the absence of cyan cavity in the 480-520 nm blue-green emission region. With 439 nm blue-light irradiation, the as-fabricated SLASSO:Ce3+ phosphor yields a broad-band cyan-green emission with the maximum emission peak positioned at 525 nm and an appropriate full width at half-maximum (fwhm) of 111 nm, capable of providing more cyan emission component without sacrificing green emission. Meanwhile, the optimal SLASSO:2%Ce3+ phosphor features CIE color coordinates of (0.3254, 0.5470) with cyan-green hue, along with a high internal quantum efficiency of up to 93%. Additionally, thermal stability measurements at different temperatures reveal that the luminescence emission intensity of the proposed phosphor retains 44% of its original integral emission intensity at 423 K with respect to room temperature, while also demonstrating an excellent color stability (ΔE = 5.4 × 10-3). This work shows that the highly efficient SLASSO:Ce3+ garnet phosphor can be utilized as a potential cyan-green-emitting phosphor for filling the cyan gap, resulting in the construction of a high-quality warm WLED with high CRI for "human-centric" sunlight-like full-spectrum solid-state illumination.

Computational Insights into the Photoinduced Dimeric Gold-Catalyzed Divergent Dechloroalkylation of gem-Dichloroalkanes with Alkenes.

The employment of dinuclear Au(I) catalysts in photomediated modern organic transformations has attracted significant attention over the past decade, which commonly demonstrates unique catalytic performance compared with the corresponding mononuclear gold complexes. Nevertheless, detailed mechanisms of dinuclear gold catalysis remain ambiguous, and further mechanistic understanding is highly desirable. Herein, computational studies were carried out to gain mechanistic insights into the photoinduced dinuclear gold-catalyzed divergent dechloroalkylation of gem-dichloroalkanes. Computational results suggest that a proton transfer from the additive, Hantzsch ester (HE), to the base, guanidine, could lead to an ionic pair complex, which is ready to undergo excitation under blue light irradiation to result in the corresponding triplet excited state. Then, the excited complex might undergo oxidative quenching with the dinuclear gold photocatalyst [AuI-AuI]2+, via a single-electron-transfer (SET) step to afford an unusual [Au1/2-Au1/2]+ dinuclear species. The corresponding mononuclear gold catalyst, [AuI]+, however, is not ready to enable the analogous step to give a [Au0] species, which might account for the unique characteristics of dinuclear gold catalysis. Subsequently, the formed [Au1/2-Au1/2]+ intermediate could trigger a Cl-atom transfer from dichloromethane in an inner-sphere manner to furnish a critical chloromethyl radical. Next, the resulting chloromethyl radical could attack the alkenyl moiety of substrates to generate the corresponding alkyl radicals. Then, three possible mechanistic pathways were explored to rationalize the substrate-dependent divergent transformations in this protocol. The main factors responsible for the diversified transformations were discussed.

Visible Light-Promoted Regioselective Benzannulation of Vinyl Sulfoxonium Ylides with Ynoates.

Herein, we report a highly regioselective [4 + 2]-annulation of vinyl sulfoxonium ylides with ynoates under light-mediated conditions. The reaction proceeds through the new dienyl sulfoxonium ylide, which undergoes photolysis under blue light irradiation to give highly substituted naphthalene scaffolds. The method presented here operates at room temperature and does not require the addition of an external photosensitizer. The in situ-generated dienyl sulfoxonium ylide absorbs light and acts as a photosensitizer for the formation of arenes. The synthetic potential of these benzannulations was further illustrated by various synthetic transformations and a scale-up reaction. Moreover, control experiments and quantum chemical calculations reveal the mechanistic details of the developed reaction.

Blue-light irradiation induced partial nitrification

The role of ray radiation from the sunlight acting on organisms has long-term been investigated. However, how the light with different wavelengths affects nitrification and the involved nitrifiers are still elusive. Here, we found more than 60 % of differentially expressed genes (DEGs) in nitrifiers were observed under irradiation of blue light with wavelengths of 440–480 nm, which were 13.4 % and 20.3 % under red light and white light irradiation respectively. Blue light was more helpful to achieve partial nitrification rather than white light or red light, where ammonium oxidization by ammonia-oxidizing archaea (AOA) with the increased relative abundance from 8.6 % to 14.2 % played a vital role. This was further evidenced by the enhanced TCA cycle, reactive oxygen species (ROS) scavenge and DNA repair capacity in AOA under blue-light irradiation. In contrast, nitrite-oxidizing bacteria (NOB) was inhibited severely to achieve partial nitrification, and the newly discovered encoded blue light photoreceptor proteins made them more sensitive to blue light and hindered cell activity. Ammonia-oxidizing bacteria (AOB) expressed genes for DNA repair capacity under blue-light irradiation, which ensured their tiny impact by light irradiation. This study provided valuable insights into the photosensitivity mechanism of nitrifiers and shed light on the diverse regulatory by light with different radiation wavelengths in artificial systems, broadening our comprehension of the nitrogen cycle on earth.

A versatile food packaging film fabricated by gallic acid/octanoic acid grafted chitosan: Preparation, characterization, high-efficient cells capture and photodynamic eradication of bacteria and biofilms

This study aims to construct a versatile film fabricated by gallic acid/octanoic acid-grafted-chitosan (GA/OA-g-CS) for high photodynamic antibacterial and antibiofilm efficacy with blue light (BL, 420 nm) irradiation. First, the GA/OA-g-CS conjugates were synthesized by a green radical-induced grafting reaction in the redox system composed of ascorbic acid/hydroxyl peroxide (AA/H2O2). Then, the molecular structural and physicochemical characteristics of the conjugates, as well as the mechanisms of the free radical grafting reaction were further studied. Ascorbate radical (Asc•−) and hydroxyl radical (•OH) were produced in the AA/H2O2 system, and •OH reacted with CS to produce CS macro-radicals (•CS). UV–vis, Fourier transform infrared, nuclear magnetic resonance spectroscopy, X-ray diffraction (XRD), and thermogravimetric analysis confirmed that GA/OA was attached onto CS through •OH. Notably, the films constructed by GA/OA-g-CS exhibited robust multi-modal functionalities, including intriguing bacteria-capturing and contact-killing, as well as BL-driven photodynamic sterilization, ascribed to the inherent merits based on the massive photoactive GA, hydrophobic and high membrane affinity of OA within the CS chains. As a result, the GA/OA-g-CS (2:1) film plus BL irradiation can efficiently eradicate Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and their biofilms. After BL irradiation for 30 min, the GA/OA-g-CS (2:1) film showed a reduction of 5.2 Log CFU/mL against E. coli. More importantly, the film with BL irradiation can efficiently not only inhibit the formation of biofilms but also jeopardize the matured biofilms. This research provides a potential platform and promises for applications in active and intelligent food packaging.