Background: Anemia is still a major health problem in females of both rural and urban areas of the developingcountries like India. The objective of the present study was to find out the hematological parameters and bloodindices among adult females in rural and urban areas of Patancheru (Mandal), Telangana and correlate to type ofanemia, degree of anemia, and percentage of anemia in both rural as well as urban areas in adult females.Methods: A cross-sectional study was conducted among adult female subjects of age group 18-35 years comprisedof n=100 subjects selected from rural area and n=100 subjects from urban areas of Patancheru (Mandal),Telangana.The hematological parameters, Hb gm%, MCV (fl), RBC count/mm3,MCH(pg), PCV(%),MCHC(gm/dl) wereinvestigated by Hematology Analyzer-automated blood counter.Results: among the total sample of n=200 adult female subjects of age group 18-45 years, the overall prevalence ofanemia was observed 58%, recording 60% in rural area females and 56% in adult female subjects of urban area. Theoverall microcytic anemia was observed maximum (40%), recording 70% in rural area subjects and 67% in urbanarea subjects. The overall macrocytic and normocytic anemias were recorded 9% each. The macrocytic anemia wasrecorded 13.5% in rural area and 17.8% in urban area. The normocytic anemia’s was recorded 16.6% in rural areaand 14.2% in urban area adult females. Both parameters of urban and Rural females were compared and there washighly Significant P Value (P<0.001).Conclusion: The present study indicated that prevalence of anaemia is slightly higher among rural adult femalesubjects of age group 18-35 years compared to urban area female subjects. The current study results suggests, needof due emphasis on iron and folic acid supplementation and education on consumption of iron rich foods, so as tobring down the prevalence of anemia among the adult females