Iron Oxide-based Nanoparticles Research Articles

Biological activities of iron oxide-based magnetic nanoparticles

Biological activities of iron oxide-based magnetic nanoparticles

Advances in Atherosclerosis Theranostics Harnessing Iron Oxide-Based Nanoparticles.

Atherosclerosis, a multifaceted chronic inflammatory disease, has a profound impact on cardiovascular health. However, the critical limitations of atherosclerosis management include the delayed detection of advanced stages, the intricate assessment of plaque stability, and the absence of efficacious therapeutic strategies. Nanotheranostic based on nanotechnology offers a novel paradigm for addressing these challenges by amalgamating advanced imaging capabilities with targeted therapeutic interventions. Meanwhile, iron oxide nanoparticles have emerged as compelling candidates for theranostic applications in atherosclerosis due to their magnetic resonance imaging capability and biosafety. This review delineates the current state and prospects of iron oxide nanoparticle-based nanotheranostics in the realm of atherosclerosis, including pivotal aspects of atherosclerosis development, the pertinent targeting strategies involved in disease pathogenesis, and the diagnostic and therapeutic roles of iron oxide nanoparticles. Furthermore, this review provides a comprehensive overview of theranostic nanomedicine approaches employing iron oxide nanoparticles, encompassing chemical therapy, physical stimulation therapy, and biological therapy. Finally, this review proposes and discusses the challenges and prospects associated with translating these innovative strategies into clinically viable anti-atherosclerosis interventions. In conclusion, this review offers new insights into the future of atherosclerosis theranostic, showcasing the remarkable potential of iron oxide-based nanoparticles as versatile tools in the battle against atherosclerosis.

SPIONs: Superparamagnetic iron oxide-based nanoparticles for the delivery of microRNAi-therapeutics in cancer.

Non-coding RNA (ncRNA)-based therapeutics that induce RNA interference (RNAi), such as microRNAs (miRNAs), have drawn considerable attention as a novel class of targeted cancer therapeutics because of their capacity to specifically target oncogenes/protooncogenes that regulate key signaling pathways involved in carcinogenesis, tumor growth and progression, metastasis, cell survival, proliferation, angiogenesis, and drug resistance. However, clinical translation of miRNA-based therapeutics, in particular, has been challenging due to the ineffective delivery of ncRNA molecules into tumors and their uptake into cancer cells. Recently, superparamagnetic iron oxide-based nanoparticles (SPIONs) have emerged as highly effective and efficient for the delivery of therapeutic RNAs to malignant tissues, as well as theranostic (therapy and diagnostic) applications, due to their excellent biocompatibility, magnetic responsiveness, broad functional surface modification, safety, and biodistribution profiles. This review highlights recent advances in the use of SPIONs for the delivery of ncRNA-based therapeutics with an emphasis on their synthesis and coating strategies. Moreover, the advantages and current limitations of SPIONs and their future perspectives are discussed.

Iron Oxide-Based Magnetic Nanoparticles: Magnetic Properties and applications in Medicine

As nanopartículas (NPs) à base de óxido de ferro, como magnetita e maguemita, têm grande destaque em diversas áreas da ciência, inclusive na medicina. Há décadas, elas são usadas na reposição de ferro para pacientes com anemia, como agentes de contraste em ressonância magnética (MRI – “Magnetic Resonance Imaging”), na entrega de agentes terapêuticos mediada ou não por gradiente de campo magnético, em terapias térmicas foto e magnetoestimuladas, entre outras. Neste artigo, apresentamos suas propriedades magnéticas. Discutimos de forma breve seu ordenamento ferrimagnético, a importância da anisotropia magnética e sua relação com a histerese magnética (modelo de Stoner-Wohlfarth) e o regime superparamagnético. As diferenças entre superparamagnetismo e paramagnetismo são enfatizadas, além da importância da estabilidade coloidal dessas nanoestruturas para aplicações biomédicas. Finalmente, diversas aplicações biomédicas são discutidas, entre elas hipertermia magnética, terapia fototérmica, imunoterapia e uma nova técnica de imagem com potencial clínico, conhecida como “Magnetic Particle Imaging” (MPI).

Iron Oxide-Based Nanoparticles for Fast-Response Humidity Sensing, Real-Time Respiration Monitoring, and Noncontact Sensing

Iron Oxide-Based Nanoparticles for Fast-Response Humidity Sensing, Real-Time Respiration Monitoring, and Noncontact Sensing

Scalable diafiltration process for the purification of a colloidal dispersion of ultrasmall iron (oxyhydr)oxide-based nanoparticles

The process description for large-scale production of iron (oxyhydr)oxide nanoparticles is currently receiving greater attention, with cost-effective industrial production usually crucial for economic application. In this context, the purification step is often price-determining in the production process. Dialysis as membrane-assisted purification is one of the most widely used methods in the case of iron (oxyhydr)oxide nanoparticles but has very limited suitability for large-scale production. This study aims to demonstrate for the first time that cross–flow filtration is a suitable alternative for the industrial production of ultrasmall iron (oxyhydr)oxide nanoparticles. This study includes the development of a concentration and diafiltration process for ultrasmall iron (oxyhydr)oxide nanoparticles on a laboratory scale, upscaling for the purification of 75 kg nanoparticle suspension, and comparison with dialysis purification.In summary, the optimized filtration process is characterized by a consistent and high product quality and high product yield, comparable to dialysis purification and suitable for pilot-scale production. Finally, the results demonstrated that the developed process is an economically attractive alternative to dialysis. The processing time is reduced by 79 %, the water consumption is 96 % lower, and 66 % of the material costs are saved.

Abstract P3-08-02: Prospective Use of Feline Mammary Carcinomas to Study MiR-10b and Metastasis

Abstract Despite many advances in cancer treatment, metastatic disease is estimated to be responsible for 90% of all cancer-related deaths. Current treatments for metastatic disease target various aspects of carcinogenesis but not specifically the metastatic process, representing a major unmet clinical need. MicroRNA-10b, a small non-coding RNA, offers tremendous potential as a treatment target for metastatic disease. It is implicated in invasion, migration, and viability of metastatic cells across a variety of cancer types, and it is upregulated in metastases compared to their matched primary tumor, establishing miR-10b as a potential treatment target unique to metastatic niche. We have previously developed a therapeutic targeting miR-10b in metastases and tested it in murine models of metastatic breast cancer. This therapeutic, consisting of an anti-miR-10b antisense oligonucleotide conjugated to iron oxide-based nanoparticles, prevents metastasis and eradicates pre-existing metastases in murine breast cancer models. With an outlook to clinical translation of our approach, we seek to test our therapeutic strategy in larger animal models. Feline mammary carcinomas are considered by most to be the best large animal model for human breast cancer due to similarities such as relative age of onset, histopathology, metastatic patterns, and treatment response. To support the use of this model with our therapeutic, we investigated the characteristics of miR-10b expression in spontaneous metastatic breast cancer in companion cats. Archival blocks of matched primary tumors and metastatic lymph nodes from companion cats diagnosed with mammary carcinoma (n=9, 44%TNBC, 56%HER2+) were obtained from the tissue bank of the Michigan State University (MSU) Veterinary Diagnostic Laboratory (VDL). Tissues were analyzed for miR-10b and its target HOXD10 expression using qRT-PCR and in situ hybridization. qRT-PCR revealed that miR-10b expression was significantly upregulated in 55.5% of lymph node metastases compared to their matched primary tumor, mirroring findings in human metastatic cancer. This was validated by qRT-PCR for HOXD10 gene expression (a direct target of miR-10b), which was significantly downregulated in these metastases compared to their matched primary tumor. In situ hybridization demonstrated that miR-10b expression was increased at the invasive edge of tumors and in actively invading cells, suggesting miR-10b plays a similar role in invasion in feline breast cancer as it does in human breast cancer. Altogether, these findings support the use of feline mammary carcinomas as a model of human breast cancer and as an excellent candidate for treatment with our therapeutic. Citation Format: Alan Halim, N. Anna Savan, Paulo Vilar Saavedra, Vilma Yuzbasiyan-Gurkan, Matti Kiupel, Lorenzo Sempere, Anna Moore. Prospective Use of Feline Mammary Carcinomas to Study MiR-10b and Metastasis [abstract]. In: Proceedings of the 2022 San Antonio Breast Cancer Symposium; 2022 Dec 6-10; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2023;83(5 Suppl):Abstract nr P3-08-02.

Inductive Thermal Effect on Thermoplastic Nanocomposites with Magnetic Nanoparticles for Induced-Healing, Bonding and Debonding On-Demand Applications

In this study, the heating capacity of nanocomposite materials enhanced with magnetic nanoparticles was investigated through induction heating. Thermoplastic (TP) matrices of polypropylene (PP), thermoplastic polyurethane (TPU), polyamide (PA12), and polyetherketoneketone (PEKK) were compounded with 2.5–10 wt.% iron oxide-based magnetic nanoparticles (MNPs) using a twin-screw extrusion system. Disk-shape specimens were prepared by 3D printing and injection molding. The heating capacity was examined as a function of exposure time, frequency, and power using a radio frequency (RF) generator with a solenoid inductor coil. All nanocomposite materials presented a temperature increase proportional to the MNPs’ concentration as a function of the exposure time in the magnetic field. The nanocomposites with a higher concentration of MNPs presented a rapid increase in temperature, resulting in polymer matrix melting in most of the trials. The operational parameters of the RF generator, such as the input power and the frequency, significantly affect the heating capacity of the specimens, higher input power, and higher frequencies and promote the rapid increase in temperature for all assessed nanocomposites, enabling induced-healing and bonding/debonding on-demand applications.

Magnetic Resonance Imaging and Iron-oxide Nanoparticles in the era of Personalized Medicine.

Medical imaging is an important factor for diagnosis. It can be used to diagnose patients, differentiate disease stages, and monitor treatment regimens. Although different imaging technologies are available, MRI is sensitive over other imaging modalities as it is capable of deep tissue penetration allowing to image the anatomical, structural, and molecular level of diseased organs. Thus, it can be used as screening tool for disease staging. One of the important components of imaging is contrast agents which are used to increase the sensitivity of MRI technology. While different types of contrast agents are available, iron-oxide based nanoparticles (IONPS) are widely used as these are easy to formulate, functionalize, biocompatible and cost effective. In addition to its use as contrast agents, these have been used as drug carriers for the treatment of different types of diseases ranging from cancer, cardiovascular diseases, neurological disorders, autoimmune diseases, and infectious diseases. For the last two decades, there has been advancement in nanotheranostics, where IONPs are formulated to carry drug and be used as contrast agents in one system so that these can be used for image-guided therapy and monitor real-life treatment response in diseased tissue. This technology can be used to stratify patients into responders and non-responders and reduce adverse drug toxicity and lead to a tailored treatment. However, success of nanotheranostics depends on several factor, including identification of disease associated biomarkers that can be targeted on IONPs during formulation. While many challenges exist for the clinical translation of nanotheranostics, it still has the potential to be implemented in personalized treatment strategy. In this review article, we discussed the use of MRI technology and IONPs in relation to their application in disease diagnosis and nanotheranostics application in personalized medicine.

Magnetic gelatin nanoparticles as a biocompatible carrier system for small interfering RNA in human colorectal cancer: Synthesis, optimization, characterization, and cell viability studies

Iron oxide-based nanoparticles have gained tremendous attention in developing next-generation personalized medicine modalities. Gelatin can be a good alternative for encapsulating iron oxide nanoparticles with its biocompatibility, biodegradability, low immunogenicity, and richness of functional groups. Herein, magnetic iron oxide nanoparticles (MNPs) were synthesized, coated with gelatin (Gel-MNPs), and loaded with mammalian target of rapamycin (mTOR)-silencing siRNA to induce the in vitro therapeutic effect in colorectal cancer (CRC) cells. To the best of our knowledge, this study is the first report using Gel-MNPs as siRNA carriers. We first optimized several experimental conditions for the preparation of MNPs and Gel-MNPs and the resulting optimized nanoparticles showed a narrow size and size distribution. Gelatin-coating increased the storage stability by preventing the aggregation of MNPs and did not alter the magnetic characteristics of MNPs significantly. siRNA encapsulation abilities of Gel-MNPs were determined in the range of 18.4 and 41.5% in varying siRNA amounts. Bare Gel-MNPs were highly cytocompatible against CRC cells, Caco-2, while Gel-MNPs-mTOR-siRNA exhibited a significant anticancer effect to kill these cells. Comparison with HiPerFect, a commercial siRNA transfection reagent, demonstrated that Gel-MNPs-mTOR-siRNA inhibited cell viability almost similar to or better than HiPerFect-mTOR-siRNA. Taken together, our data indicated that Gel-MNPs could potentially be used in further gene silencing approaches as a safe and multifunctional siRNA carrier candidate.

Magnetic Systems for Regenerative Medicine

Magnetic Systems for Regenerative Medicine

Effects of Fe3O4 Magnetic Nanoparticle Functionalization with Ionic Liquids and a Double-Chained Surfactant on the Pretreatment of Plasma Samples during Drug Extraction.

Ionic liquids (ILs), also known as "designer solvents," comprise a large group of compounds that can improve overall sample preparation performance due to their unique physical and chemical properties. Some of them have a comparable structure to surfactants, which can be also considered as effective extraction solvents. In this study, nine different ILs and a double-chained surfactant were investigated as potential coating materials for iron oxide-based nanoparticles (NPs) used in the pretreatment of human plasma samples. Various methods of synthesizing and functionalizing NPs were employed in fabricating the magnetic sorbents, with the physicochemical properties of the resultant extraction phases (i.e., naked NPs, NPs coated with silica, and NPs coated with silica and selected IL or surfactant) being characterized via X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, thermogravimetric analysis (TG), and transmission electron microscopy (TEM). The effectiveness of the developed NP-based extraction phases was tested by applying them for the extraction of epirubicin hydrochloride (EPI) from plasma samples, followed by analysis via liquid chromatography with fluorescence detection (LC-FL). The results showed that NPs coated with both silica and IL or silica and surfactant provided significantly higher extraction efficiency compared to naked NPs and NPs coated solely with silica. Additionally, the findings also revealed that the adsorption of analytes depends not only on the coating procedure but also on the type of coating material used to functionalize the NPs. Among the tested structures, didodecyldimethylammonium bromide provided the best performance for the functionalization of NP sorbents previously coated with silica.

A New Platform of Iron Oxide-Based Nanoparticles Assay Using GMR Chip-Based Sensor With Microcontroller

The low-cost, simple, and fast quantitative assay of magnetic nanoparticles (MNPs) is important for the development of magnetic-based immunoassay applications. However, the bulky sensing element and instruments of the recent iron oxide-based MNPs assays are still concerns that should be resolved. This article reports the new platform of an iron oxide-based MNPs label assay using the commercial giant magnetoresistance (GMR) chip AAL024, manufactured by Nonvolatile Electronics (NVE) Corporation, complemented with a basic differential amplifier circuit and Arduino microcontroller (AM) to acquire the digital output voltage. The as-prepared Fe3O4@Ag label possessed (15 ± 2.6) nm of average size and behaved as soft ferromagnetic with 52.1 emu/g of saturation magnetization, and 0.15 kOe of coercive field. Sensor performance for magnetic field detection, as well as Fe3O4@Ag nanoparticles label assay, was evaluated. The results show the implementation of a basic differential amplifier op-amp circuit succeed to magnify the sensor’s response to the magnetic field up to 23 times with the output voltage in the order of volt. The employment of the AM as a voltage supply of the GMR chip, an analog-to-digital converter, and a voltage gauge simplifies the data acquisition without the additional measuring instrument. Meanwhile, for the purpose of the Fe3O4@Ag nanoparticles label assay, the sensor revealed promising performance due to the high signal linearity and the rapid detection which is only 30 s with acceptable signal stabilities.

Iron Oxide Nanoparticles (IONPs): Synthesis, Surface Functionalization, and Targeting Drug Delivery Strategies: Mini-Review

Iron oxide-based magnetic nanoparticles (IONPs) have received remarkable attention in a wide range of applications because of their unique physicochemical properties’ inheritance to the nanoscale. Among these nanoparticles (NPs), superparamagnetic iron oxide nanoparticles (SPIONs), as powerful noninvasive NPs, are widely used in nanomedicine applications such as targeted drug/ gene delivery, magnetic separation, cancer therapy, and magnetic resonance imaging (MRI) hyperthermia because of their superparamagnetic activity and remarkable small size. The synthesis of SPIONs and surface modification of these NPs for biological applications is an interesting research topic. These NPs have high magnetic susceptibility, a single magnetic domain, and a controlled magnetic behavior due to the SPION superparamagnetic feature. This review aims to explore the recently developed synthetic routes of SPIONs and show the best parameters to prepare SPIONs using pulsed laser ablation in liquid “PLAL” for biomedical applications. Furthermore, we highlight the properties, coating, and functionalization of SPIONs and their importance for biomedical applications, including targeted drug delivery and cancer therapy.

Ecotoxicological assessment of magnetite and magnetite/Ag nanoparticles on terrestrial and aquatic biota from different trophic levels

The aim of the study is an ecotoxicological assessment of magnetite iron oxide-based nanoparticles (NPs), which have risen in popularity in the last decade, on selected terrestrial and aquatic organisms from various levels of the food chain.In the presented study various organisms, from both the terrestrial and aquatic environment, were used as targets for the assessment of NPs ecotoxicity. Plants (radish, oat), marine bacteria (A. fischeri) and crustacean (H. incongruens) were used to represent producers, decomposers, and consumers, respectively. It was found that examined NPs were harmful (to a different degree) to biota from three different trophic levels.Physicochemical characterization (size/morphology, crystallinity, composition, and magnetic properties) of the tested nanoparticles was performed by: transmission electron microscopy, X-ray diffraction, energy dispersive spectroscopy, and Mossbauer spectroscopy, respectively.Phytotoxicity was evaluated according to the OECD 208 Guideline, while acute and chronic toxicity of NPs was conducted using bioassays employing bacteria and crustacea, respectively. The phytotoxicity of all investigated iron oxide-based NPs was dependent on concentration and type of NPs formulation and was measured via biomass, seed germination, root length, shoot height, and content of plant pigments.Increasing the concentration of NPs increased phytotoxicity and mortality of aquatic organisms. Ecotoxicity of iron oxide/silver was dependent on the size and content of silver. Iron oxide NPs coated with nanosilver in a percentage ratio of 69/31 were found to be the most toxic on tested terrestrial and aquatic biota.

Effect of asphaltene adsorption on the magnetic and magnetorheological properties of heavy crude oils and Fe3O4 nanoparticles systems

An essential understanding of the adsorptive phenomena involving nanomaterials and asphaltenes has received considerable attention in oil recovery and production applications. Particularly, iron oxide-based nanoparticles have emerged as advanced materials due to their unique physicochemical properties, including thermal conductivity and superparamagnetism. On this basis, the kinetics and adsorption isotherms of magnetite (Fe3O4) nanoparticles/asphaltene systems were investigated, as well as the synergistic effect of resins acting as stabilizing colloidal agents. The results indicated rapid asphaltene adsorption that, essentially, follows the phenomenological external mass transfer (EMT) model. At the equilibrium, the asphaltene adsorption was adequately described by the monolayer Langmuir model. By these findings, it was studied for the first time the effect of the adsorbed asphaltenes on the magnetic dipolar interactions in nanoparticles/asphaltene powder state configurations, by applying a simple analytical model based on magnetic susceptibility and demagnetizing field concepts. It was proven accurately that asphaltenes coated the surfaces of the nanoparticles forming layers that stimulate a steric repulsive barrier between particles and, thus, reducing the intensity of interactions. Accordingly, a theoretical method was validated to estimate the magnetic-size distribution parameters by comparison with the physical distribution data obtained by Transmission Electron Microscopy (TEM). Finally, from the magnetically modified dynamic rheological properties, it was inferred that isotropic aggregates are naturally formed in the absence of the magnetic field, and these morphological features were not dramatically changed after the imposition of the field, leading to direct extrapolation of the modeling-based procedure for powders to real colloidal configurations applied in oil field operations.

Significant Surface Spin Effects and Exchange Bias in Iron Oxide-Based Hollow Magnetic Nanoparticles.

Exchange bias (EB) properties have become especially important in hollow magnetic nanoparticles (MNPs) due to the versatility and reduced size of these materials. In this work, we present the synthesis and study of the EB properties of iron-oxide-based hollow MNPs and their precursors Fe/iron oxide MNPs with core/void/shell structure. The two mechanisms involved in EB generation were investigated: the frozen spins present in the nanograins that form the nanoparticles and the surface spins. The effect of external parameters on the coercivity (HC), remanence (MR), exchange bias field (HEB) and frozen spins, such as cooling field (HFC) and temperature, was investigated. Both HC and HEB present a maximum threshold above which their values begin to decrease with HFC, showing a new trend of HEB with HFC and allowing modulation on demand. The existence of surface spins, present on the outer and inner surfaces, was demonstrated, and an intrinsic EB phenomenon (HEB = 444 Oe for hollow iron oxide-based MNPs of 13.1 nm) with significant magnetization (MS~50 emu/g) was obtained. Finally, core/void/shell MNPs of 11.9 nm prior to the formation of the hollow MNPs showed a similar behavior, with non-negligible HEB, highlighting the importance of surface spins in EB generation.

Investigation of the well-dispersed magnetorheological oil-based suspension with superparamagnetic nanoparticles using modified split Hopkinson pressure bar

Magnetorheological (MR) fluids are classified as smart materials whose viscoplastic characteristics change under the magnetic field. They are widely applied for dynamic energy dissipation due to their rapid thickening under the external magnetic field. In this work, the core–shell suspension of superparamagnetic iron oxide-based nanoparticles was synthesized and dispersed in silicone oil. Much effort has been made to prepare suspension meeting requirements of MR fluid. The experimental squeezing flow response was studied using a modified split Hopkinson pressure bar (SHPB) with various shear rates. Tests with modified SHPB show that MR fluid rapidly responds to the compression thickening and forming chain-like structures. MR fluid dissipates the energy generated during compression stress tests. This study presents a simple and cost-effective synthesis way suitable for MR fluid formation for its dynamic energy dissipation application.

Green Synthesis of Carbon-Encapsulated Magnetic Fe3O4 Nanoparticles Using Hydrothermal Carbonization from Rattan Holocelluloses

How to design a simple and scalable procedure for manufacturing multifunctional carbon-based nanoparticles using lignocellulosic biomass directly is a challenging task. Based on the green chemistry concept, we developed a novel one-pot solution-phase reaction to prepare carbon-encapsulated magnetic nano-Fe3O4 particles (Fe3O4@C) with a tunable structure and composition through the hydrothermal carbonization (HTC) of Fe2+/Fe3+ loaded rattan holocelluloses pretreated with ionic liquids (EmimAc and AmimCl). The detailed characterization results indicated that the Fe3O4@C synthesized from the holocelluloses pretreated with ionic liquids (ILs) under alkaline conditions tends to have a higher saturation magnetization, probably due to the increased iron ions loading. Moreover, increasing the HTC temperature led to an increased abundance of hydroxyl groups on the surface of the synthesized particles and an elevated saturation magnetization. When EmimAc-treated holocelluloses were used as the carbon precursors, well-encapsulated Fe3O4@C nanoparticles were obtained with a maximum saturation magnetization of 42.6 emu/g. This synthetic strategy, coupled with the structure of the iron carbide-based composite and the proposed mechanism, may open a new avenue for the development of carbon-encapsulated iron oxide-based magnetic nanoparticles.

Novel Nanoparticle-Based Cancer Treatment, Effectively Inhibits Lung Metastases and Improves Survival in a Murine Breast Cancer Model.

Sarah Nanoparticles (SaNPs) are unique multicore iron oxide-based nanoparticles, developed for the treatment of advanced cancer, following standard care, through the selective delivery of thermal energy to malignant cells upon exposure to an alternating magnetic field. For their therapeutic effect, SaNPs need to accumulate in the tumor. Since the potential accumulation and associated toxicity in normal tissues are an important risk consideration, biodistribution and toxicity were assessed in naïve BALB/c mice. Therapeutic efficacy and the effect on survival were investigated in the 4T1 murine model of metastatic breast cancer. Toxicity evaluation at various timepoints did not reveal any abnormal clinical signs, evidence of alterations in organ function, nor histopathologic adverse target organ toxicity, even after a follow up period of 25 weeks, confirming the safety of SaNP use. The biodistribution evaluation, following SaNP administration, indicated that SaNPs accumulate mainly in the liver and spleen. A comprehensive pharmacokinetics evaluation, demonstrated that the total percentage of SaNPs that accumulated in the blood and vital organs was ~78%, 46%, and 36% after 4, 13, and 25 weeks, respectively, suggesting a time-dependent clearance from the body. Efficacy studies in mice bearing 4T1 metastatic tumors revealed a 49.6% and 70% reduction in the number of lung metastases and their relative size, respectively, in treated vs. control mice, accompanied by a decrease in tumor cell viability in response to treatment. Moreover, SaNP treatment followed by alternating magnetic field exposure significantly improved the survival rate of treated mice compared to the controls. The median survival time was 29 ± 3.8 days in the treated group vs. 21.6 ± 4.9 days in the control, p-value 0.029. These assessments open new avenues for generating SaNPs and alternating magnetic field application as a potential novel therapeutic modality for metastatic cancer patients.