Mining activities in the iron area mining have caused serious pollution of surface water in the area, which in turn affects the normal growth and development of plants. As a restorative plant with heavy metal adsorption function, the stress response mechanism of rose to surface water in the iron-mining area remains unelucidated. In this study, we investigated the effects of surface water treatment on the phenotypic, physiological and molecular levels of the plant using rose as a material. The results showed that surface water in the Bayan Obo iron mining area caused darkening and soft rotting of rose petals, increased degree of petal damage, decreased the content of MDA (Malondialdehyde), increased the ratio of GSH/GSSG (glutathione/oxidized glutathione) and NADPH/NADP+(nicotinamide adenine dinucleotide phosphate/oxidized nicotinamide adenine dinucleotide phosphate), suggesting that the rose produces more GSH to prevent oxidative stress. Transcriptome analysis further revealed a large number of differentially expressed genes (DEGs) involved in the TCA (the tricarboxylic acid) cycle and the phenylpropanoid biosynthesis pathways under surface water stress, and these DEGs can promote the production of NADPH, which in turn positively regulates the content of GSH. Multiple peroxidase-related genes (RhPRXs) also exhibited a significant stress response in this process. In summary, our research results reflect that roses under surface water stress in the iron-mining area exhibit certain defense mechanisms at both physiological and transcriptional levels, and also provide a reference for cultivating effective rose resources for repairing pollution in mining areas and evaluating excessive iron pollution as a biological indicator.
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