Iron Content Research Articles

Supercapacitive performance of self-assembled thin film of liquid catocene/basal plane pyrolytic graphite electrode

Reasonable design of electrode material with low cost, lightweight, and excellent electrochemical properties is of great significance for future large-scale energy storage applications. Herein, we report the electrochemical and supercapacitive behaviour of the liquid redox of catocene, 2,2’-bis(ethyl-ferroceneyl) propane, self-assembled on a basal plane pyrolitic graphite electrode in comparison to the solid ferrocene thin film in aqueous sodium sulfate electrolyte. The modified electrode surfaces were evaluated to assess the iron content and the formation of thin film using scanning electron microscopy, laser-induced breakdown spectroscopy, and attenuated total reflectance method. Also, the supercapacitive performances of the related modified electrodes were assessed and compared using cyclic voltammetry and galvanostatic charge-discharge in a three-electrode system and an asymmetric two-electrode supercapacitor system. Electro­chemical results showed that the electrode processes are diffusion-controlled with battery-like behaviour, and the liquid catocene exhibits more effective interaction with the graphite surface in comparison to solid ferrocene. The catocene surface coverage on graphite is nearly 50-75 % higher than ferrocene, leading to improved interaction and charge transfer resistance, observed in electrochemical impedance spectroscopy studies. In galvanostatic charge-discharge evaluations, the supercapacitor based on catocene modified electrode shows a specific capacitance of 141.2 F g-1 at a current density of 1.0 A g-1, with a specific energy density of 56.7 Wh kg-1 at a power density of 2.9 kW kg-1.

New insights into the mechanisms of iron absorption: Iron dextran uptake in the intestines of weaned pigs through glucose transporter 5 (GLUT5) and divalent metal transporter 1 (DMT1) transporters

The purpose of this study was to gain insight into the mechanism of iron dextran (DexFe) absorption in the intestines. A total of 72 piglets (average BW = 7.12 ± 0.75 kg, male to female ratio = 1:1) weaned at 28 d of age were randomly divided into two treatment groups with six replicates for each group. The experimental diets included the basal diet supplemented with 100 mg/kg iron dextran (DexFe group) and the basal diet supplemented with 100 mg/kg FeSO4•H2O (CON group). The experiment lasted for 28 d. The piglets' intestinal iron transport was measured in vitro using an Ussing chamber. Porcine intestinal epithelial cell line (IPEC-J2) cells were used to develop a monolayer cell model that explored the molecular mechanism of DexFe absorption. Results showed that compared to the CON group, the ADG of pigs in the DexFe group was improved (P = 0.022), while the F/G was decreased (P = 0.015). The serum iron concentration, apparent iron digestibility, and iron deposition in the duodenum, jejunum, and ileum were increased (P < 0.05) by dietary DexFe supplementation. Piglets in the DexFe group had higher serum red blood count, hemoglobin, serum iron content, serum ferritin and transferrin levels and lower total iron binding capacity (P < 0.05). In the Ussing chamber test, the iron absorption rate of the DexFe group was greater (P < 0.001) than the CON group, and there was no significant difference between the DexFe group and the glucose group (P > 0.05). Furthermore, when compared to the CON group, DexFe administration improved (P < 0.05) SLC2A5 gene and glucose transporter 5 (GLUT5) protein expression but had no effect (P > 0.05) on SLC11A2 gene or divalent metal transporter 1 (DMT1) protein expression. Once the GLUT5 protein was suppressed, the iron transport rate and apparent permeability coefficient were decreased (P < 0.05) in IPEC-J2 monolayer cell models. The findings suggest the effectiveness of DexFe application in weaned piglets and revealed for the first time that DexFe absorption in the intestine is closely related to the glucose transporter GLUT5 protein channel.

In-depth transcriptome and physiological function analysis reveals the toxicology of sodium fluoride in the fall webworm Hyphantria cunea

Fluoride is an environmental pollutant that severely injures various organisms in ecosystems. Herein, the non-target organism, fall webworm (Hyphantria cunea), was used to determine the toxicological mechanism of NaF exposure. In this study, H. cunea exposed to NaF showed significant declines in growth and reproduction. The authors conducted RNA sequencing on adipose bodies and midgut tissues from NaF-exposed H. cunea larvae to uncover the toxicological mechanisms. The results showed that extracellular matrix-receptor interaction, pentose and glucuronate interconversions, fatty acid biosynthesis, and ferroptosis might contribute to NaF stress. NaF significantly decreased the antioxidant level, nitrous oxide synthase activity, and NO content, while significantly increasing lipid peroxidation. NaF induced significant changes in the expression of energy metabolism genes. However, the triglyceride content was significantly decreased and the lipase enzyme activity was significantly increased. Moreover, the expression levels of light and heavy chains of ferritin were inhibited in NaF-exposed H. cunea. NaF caused ferritin Fe2+overload in FerHCH1 and FerLCH knockdown H. cunea larvae, activated reactive oxygen species, and reduced the total iron content, eventually increasing the mortality H. cunea larvae. This study identified the toxicological mechanisms of NaF in lipid synthesis and energy metabolism in H. cunea, providing a basis for understanding the molecular mechanisms of NaF toxicity and developing pollution control strategies.

Formation mechanism of blue pigment in boiled lotus rhizome discs: Insight into the chelation of polyphenols and iron

Boiled lotus rhizome discs (BLRDs), as common processed products of lotus rhizome, have gained increasing attention from consumers and food manufacturers. However, the blue pigment formed during boiling affects its appearance and reduces the appetite of BLRDs. In this study, the effects of polyphenols and iron contents on blue pigment formation in BLRDs in different regions and months were investigated. Results revealed that blue variation was more serious in March and April of the second year in Wuhan, and polyphenols and iron contents in these two months were significantly higher than those in other months. Then, UPLC and UV–Vis analysis showed that polyphenols causing the formation of blue pigment in BLRDs were L-dopa, gallocatechin, catechin, epigallocatechin, chlorogenic acid and epicatechin, among which L-dopa (52.450 mg/100 g in fresh lotus rhizome (FLR)) and gallocatechin (36.210 mg/100 g in FLR) possessed the greatest effect. Moreover, the ESI-Q-TOF-MS analysis of L-dopa-iron chelate and gallocatechin-iron chelate suggested that the blue pigment of BLRDs was mainly in the form of bis-complexes under boiling conditions. The study on formation mechanism of blue pigment in BLRDs can provide a reference for lotus rhizome processing.

Qishen granule alleviates doxorubicin-induced cardiotoxicity by suppressing ferroptosis via nuclear erythroid factor 2-related factor 2 (Nrf2) pathway

Ethnopharmacological relevanceThe clinical usage of doxorubicin (DOX) is greatly constrained because of its side effects, especially cardiotoxicity. Studies have suggested that ferroptosis of cardiomyocytes is one of the important causes of doxorubicin-induced cardiotoxicity (DIC). Up-regulating Nuclear erythroid factor 2-related factor 2 (Nrf2) is a potential way to prevent ferroptosis associated with DIC. Qishen granules (QSG) has been shown cardioprotective effects on various cardiovascular diseases, including DIC. However, the mechanism of QSG to prevent and treat DIC are not fully understood. Aim of the studyThe main purpose of this work is to probe whether QSG can mitigate DIC by inhibiting ferroptosis, and whether QSG suppresses ferroptosis via Nrf2 pathway. Materials and methodsThe effects of QSG on mitigating DIC and the potential targets of QSG were investigated in a DIC mice model. The cardiac function of mice was monitored by echocardiography. Transmission electron microscopy was used to assess mitochondrial damage. ROS content was measured by dihydroethidium (DHE) staining. The glutathione (GSH) and malondialdehyde (MDA) levels in cardiac tissue were detected by kits to evaluate cellular oxidative stress. The accumulation and nuclear translocation of Nrf2 was detected by immuno-fluorescence. Ferroptosis analysis was determined by tissue iron content and key proteins in Nrf2 pathway were measured by western blotting. The anti-oxidant and anti-ferroptosis mechanisms of QSG were explored in vitro studies. Delivery of Nrf2 small interfering RNA (siRNA) to H9c2 cells aimed to investigate whether QSG could prevent DIC through Nrf2 signaling pathway. The protective effects of QSG on mito-chondrial function and free iron were measured by MitoSOX™ Red and FerroOrange staining assays, respectively. ResultsIn vivo, QSG could improve heart function and suppress ferroptosis in DIC mice. DOX-induced ROS production decreased after QSG treatment. The accumulation of free iron and MDA induced by DOX was suppressed with QSG treatment. The level of GSH increased after QSG intervention. QSG also protected against DOX-induced mitochondrial structural damage. Meanwhile, QSG promoted the expression of Nrf2 pathway-related proteins, thereby resisting ferroptosis. In vitro, QSG exerted anti-oxidant and anti-ferroptosis effects. QSG promoted the nuclear-translocation of Nrf2. In addition, interference with Nrf2 attenuated the regulatory effect of QSG on free iron content and mitochondrial ROS production. Moreover, Nrf2 knockdown weakened the anti-ferroptosis effects of QSG and inhibited the expressions of key proteins in Nrf2 pathway. ConclusionThe results of this study first illustrated that QSG could alleviate DIC by suppressing ferroptosis via Nrf2 pathway. Nrf2 may be a potential key target for QSG to prevent and treat DIC.

Relationship between serum iron, zinc, calcium, and HIF-1a-comparative analysis of 2 regions and 4 ethnic groups in China.

Altitude illness has serious effects on individuals who are not adequately acclimatized to high-altitude areas and may even lead to death. However, the individualized mechanisms of onset and preventive measures are not fully elucidated at present, especially the relationship between altitude illness and elements, which requires further in-depth research. Fresh serum samples were collected from individuals who underwent health examinations at the two hospitals in Xining and Sanya between November 2021 and December 2021. The blood zinc (Zn), iron (Fe), and calcium (Ca) concentrations, as well as hypoxia-inducible factor 1-alpha (HIF-1α) concentrations, were measured. This study conducted effective sample size estimation, repeated experiments, and used GraphPad Prism 9.0 and IBM SPSS version 19.0 software for comparative analysis of differences in the expression of elements and HIF-1α among different ethnic groups, altitudes, and concentration groups. Linear regression and multiple linear regression were employed to explore the relationships among elements and their correlation with HIF-1α. This study included a total of 400 participants. The results from the repeated measurements indicated that the consistency of the laboratory test results was satisfactory. In terms of altitude differences, except for Fe (p = 0.767), which did not show significant variance between low and high altitude regions, Zn, Ca, and HIF-1α elements all exhibited notable differences between these areas (p < 0.0001, p = 0.004, and p < 0.0001). When grouping by the concentrations of elements and HIF-1α, the results revealed significant variations in the distribution of zinc among different levels of iron and HIF-1α (p < 0.05). The outcomes of the linear regression analysis demonstrated that calcium and zinc, iron and HIF-1α, calcium and HIF-1α, and zinc and HIF-1α displayed substantial overall explanatory power across different subgroups (p < 0.05). Finally, the results of the multiple linear regression analysis indicated that within the high-altitude population, the Li ethnic group in Sanya, and the Han ethnic group in Sanya, the multiple linear regression model with HIF-1αas the dependent variable and elements as the independent variables exhibited noteworthy overall explanatory power (p < 0.05). The levels of typical elements and HIF-1α in the blood differ among various altitudes and ethnic groups, and these distinctions may be linked to the occurrence and progression of high-altitude illness.

HRAS Induces Ferroptosis through Upregulating HSPB1 in Hepatocellular Carcinoma.

The aim of this study is to explore the mechanism of HRAS and HSPB1 in ferroptosis. Primary liver cancer is the third leading cause of tumor death worldwide. Hepatocellular carcinoma (HCC) constitutes 75%-85% of cases of primary liver cancer. HRAS and HSPB1 co-express in multiple cells and participate in tumor progression regulation. However, their expression regulation and role in HCC have not been reported. We investigated the effects of HRAS and HSPB1 on ferroptosis in in vitro experiments. Here, the role and mechanism of HRAS and HSPB1 on ferroptosis were investigated by transfecting the specific siRNA or overexpressing plasmids in HCC cells. Bioinformatics analysis proved that HRAS and HSPB1 were highly expressed in HCC tissues and associated with poor prognosis of patients with HCC. In vitro, HRAS overexpression reduced the level of intracellular iron, ROS, and MDA production in HCC cells. Mechanistically, HRAS increased GPX4 expression and decreased the levels of ACSL4 and P53. HRAS also increased HSPB1 expression, and HRAS knockdown downregulated HSPB1 levels in HCC cells. Importantly, overexpression of HSPB1 reversed HRAS-increased concentration of iron, MDA, and ROS and eliminated HRAS-induced ferroptosis. Moreover, HRAS enhanced the proliferation and invasion by targeting HSPB1. The regulation of HSPB1 by HRAS enhanced the resistance of HCC cells to ferroptosis. HRAS promoted proliferation and invasion by upregulating HSPB1. This research provides a new potential strategy for HCC treatment.

Preparation and Performance Evaluation of Polysaccharide-Iron Complex of Eucommia ulmoides.

An innovative iron supplement crucial for treating iron-deficiency anemia was developed in this study. Polysaccharide was extracted from Eucommia ulmoides leaves using a microwave-assisted hot water method, and subsequently, the polysaccharide-iron complex was synthesized through co-thermal synthesis with FeCl3. The physicochemical properties, structure, and thermal stability of the complex were analyzed using FE-SEM, SEC-MALLS, FT-IR, XRD, and DSC techniques. Furthermore, the antioxidant activity of the polysaccharide-iron complex was evaluated through an experiment in vitro. The results revealed that the polysaccharide-iron complex had an iron content of 6.1% and an average particle size of 860.4 nm. The microstructure analysis indicated that the polysaccharide-iron complex possessed a flaky morphology with smooth and compact surfaces. Moreover, the formation of the Fe3+ complex did not alter the structural framework of the polysaccharide; instead, it enhanced the polysaccharide's thermal stability. Compared to traditional iron supplements, the E. ulmoides-derived polysaccharide-iron complex demonstrated significant antioxidant activity. Therefore, this novel compound exhibits significant potential as a viable iron supplement.

Assessment of the Ferroptosis Regulators: Glutathione Peroxidase 4, Acyl-Coenzyme A Synthetase Long-Chain Family Member 4, and Transferrin Receptor 1 in Patient-Derived Endometriosis Tissue.

Ferroptosis, an iron-dependent form of non-apoptotic cell death, plays a pivotal role in various diseases and is gaining considerable attention in the realm of endometriosis. Considering the classical pathomechanism theories, we hypothesized that ferroptosis, potentially driven by increased iron content at ectopic sites, may contribute to the progression of endometriosis. This retrospective case-control study provides a comprehensive immunohistochemical assessment of the expression and tissue distribution of established ferroptosis markers: GPX4, ACSL4, and TfR1 in endometriosis patients. The case group consisted of 38 women with laparoscopically and histologically confirmed endometriosis and the control group consisted of 18 women with other gynecological conditions. Our study revealed a significant downregulation of GPX4 in stromal cells of endometriosis patients (M = 59.7% ± 42.4 versus 90.0% ± 17.5 in the control group, t (54) = -2.90, p = 0.005). This finding aligned with slightly, but not significantly, higher iron levels detected in the blood of endometriosis patients, using hemoglobin as an indirect predictor (Hb 12.8 (12.2-13.5) g/dL versus 12.5 (12.2-13.4) g/dL in the control group; t (54) = -0.897, p = 0.374). Interestingly, there was no concurrent upregulation of TfR1 (M = 0.7 ± 1.2 versus 0.2 ± 0.4 for EM, t (54) = 2.552, p = 0.014), responsible for iron uptake into cells. Our empirical findings provide support for the involvement of ferroptosis in the context of endometriosis. However, variances in expression patterns within stromal and epithelial cellular subsets call for further in-depth investigations.

Assessment of Qualitative and Quantitative Damage by Coffee Bean Weevil (Araecerus fasciculatus) on Selected Dried Chips

Insect pests are important biotic organisms that cause significant damage to food crops. In a bid to prevent deterioration, farmers processed them into dried chips and thereafter consumed or stored. Coffee bean weevil, Araecerus fasciculatus is a major insect pest causing severe economic losses during storage in Nigeria. The study investigated the qualitative and quantitative damage of selected dried chips due to attack of A. fasciculatus. Quantitative weight loss of cassava, potato, water yam, white yam and plantain was assessed by placing two sets of 200g sample of each dried chips materials in 2 litres capacity Kilner jars, covered with muslin cloth for aeration and to stop other pests from infesting them. In the first set, twenty-day old adult A. fasciculatus were introduced into the Kilner jars and the sets up arranged in the laboratory using a Completely Randomized Design (CRD), at temperature of 27±3oC, 65±5% relative humidity and 12 hours photophase. The second sets up were left uninfested to serve as control. The experiment was replicated four times. The set up were reweighed at 30, 60 and 90 days after infestation to determine the weight loss in percentage. Qualitative loss was evaluated through proximate and mineral analyses. The frass amount generated by the A. fasciculatus was measured after 90 days of infestation. Plantain and cassava experienced highest percentage damage as well as highest amount of frass production due to preference, texture and their composition. Proximate and mineral analyses of the dried chips after 90 days of infestation showed significant reduction in the content of ash, crude fibre, dry matter, calcium, potassium, iron, phosphorus and increase in fat, crude protein and moisture content compared to initial contents. Araecerus fasciculatus caused significant damage to dried chips due to continuous feeding on them and also showed highest preference for plantain and cassava while the least preference was on sweet potato.

Prediction of As and Cd dissolution in various soils under flooding condition

Although the mobility of arsenic (As) and cadmium (Cd) in soils during the flooding-drainage process has been intensively studied, predicting their dissolution among various soils still remains a challenge. After comprehensively monitoring multiple parameters related to As and Cd dissolution in 8 soils for a 60-day anaerobic incubation, the redundancy analysis (RDA) and structural equation model (SEM) were employed to identify the key factors and influencing pathways controlling the dynamic release of As and Cd. Results showed that pH alone explained 90.5 % Cd dissolution, while the dissolved-Fe(II) and 5 M-HCl extractable Fe(II) jointly only explained 50.6 % As dissolution. After data normalization, the ratio of Fe(II) to 5 M-HCl extracted total Fe (i.e. FetotII/Fetot) significantly improved the correlation to R2 = 0.824 (p < 0.001) with a fixed slope of 0.393 among the 8 soils. Our results highlight the crucial role played by the reduction degree of total iron contents in determining both the reduction and dissolution of As during flooding. In contrast, dissolved-Fe(II) was too vulnerable to soil properties to be a stable indicator of As dissolution. Therefore, we propose to replace the dissolved-Fe(II) with this novel ratio as the key index to quantitatively assess the kinetic change of As solubility potential across various soils under flooding conditions.

Cytotoxic and radical activities of metal-organic framework modified with iron oxide: Biological and physico-chemical analyses

Metal-organic framework (MOF) modified with iron oxide, Fe3O4-MOF, is a perspective drug delivery agent, enabling magnetic control and production of active hydroxyl radicals, •OH, via the Fenton reaction. This paper studies cytotoxic and radical activities of Fe-containing nanoparticles (NPs): Fe3O4-MOF and its components – bare Fe3O4 and MOF (MIL-88B). Luminous marine bacteria Photobacteriumphosphoreum were used as a model cellular system to monitor bioeffects of the NPs. Neither the NPs of Fe3O4-MOF nor MOF showed cytotoxic effects in a wide range of concentrations (<10 mg/L); while Fe3O4 was toxic at >3·10−3 mg/L. The NPs of Fe3O4 did not affect the bacterial bioluminescence enzymatic system; their toxic effect was attributed to cellular membrane processes. The integral content of reactive oxygen species (ROS) was determined using a chemiluminescence luminol assay. Bacteria mitigated excess of ROS in water suspensions of Fe3O4-MOF and MOF, maintaining bioluminescence intensity closer to the control; this resulted in low toxicity of these NPs. We estimated the activity of •OH radicals in the NPs samples with physical and chemical methods – spin capture technology (using electron paramagnetic resonance spectroscopy) and methylene blue degradation. Physico-chemical interpretation of cellular responses is provided in terms of iron content, iron ions release and •OH radical production.

Assessment of vitamins and minerals of rats fed with plantain-maize pudding prepared using edible plant leaves and metallic plates

Cooking materials are important factors that basically intended to provide covering, protection and consumers’ choice of food products. The objective of the study was to determine the vitamin profile and minerals in the serum of rats fed maize-plantain pudding cooked using different leaves and metallic plates. Different maize-plantain pudding samples were prepared using plantain and maize flour blended together 1:1 ratio. In the animal study, thirty rats were divided into six groups of five rats each. Rats in Group A served as control. Group B rats were given Tween 80. Rats in Groups C, D, E and F were fed pudding prepared using plantain leaves, ginger leaves, aluminium plates and cast iron plates respectively. The rats in each group were allowed free access to feed and clean drinking water throughout the period of the experiment for 28 days. Mineral analysis was done using an atomic absorption spectrophotometer. Vitamin profile was determined using high performance liquid chromatography (HPLC). The results showed that serum minerals (calcium, potassium, magnesium, sodium, iron and phosphorus) and total vitamin content were significantly higher in rats fed with pudding prepared using ginger leaves when compared with plantain leaves, cast iron plate and aluminium plate. The serum iron content was significantly higher in rats fed pudding prepared using cast iron plate compared to other groups. Therefore, the use of locally accessible and consumable leaves and cast iron plates or utensils may be encouraged in diet preparation

Ferritin at different iron loading: From biological to nanotechnological applications

The characterization of the structure of ferritin in solution and the arrangement of iron stored in its cavity are intriguing subjects for both cell biology and applied science, since the protein structure, stability, and easiness of production make it an ideal tool for biomedical applications. We characterized the ferritin structure over a wide range of iron loadings by visible light, X-ray, and neutron scattering techniques. We found that the arrangement of iron ions inside the protein cage resulted in a more disposable arrangement at lower loading factors and then in a crystalline structure. At very high iron content the inner core is composed of magnetite more than ferrihydrite, and the shell of the protein is elastically deformed by the iron crystal growth in an ellipsoidal arrangement. The application of an external radiofrequency (RF) magnetic field affected ferritins at low iron loading factors. Notably the RF modified the iron disposition towards a more dispersed arrangement. The structural characterization of the ferritin at different LFs and in presence of magnetic fields provides useful insights into their physiological behaviour and can help in the design and fine-tuning of ferritin-based nanosystems for biotechnological applications.

The Correlation between Iron Level and Schizophrenia

Schizophrenia is a complex psychiatric condition that, if not adequately treated, can affect functional limitations. The exact etiopathogenesis of schizophrenia remains unknown. Research suggests an interaction between many factors, including genetic susceptibility, environment and psychological processes. Specific authors describe the association of a valuable mineral in the human body, iron, with pathophysiological mechanisms and related etiological factors in the development of the severe mental illness of schizophrenia.Iron has important roles in the human body and affects various physiological processes. Some studies have shown a connection between the dysregulation of iron levels and the development of different mental disorders, including schizophrenia. Abnormal levels of iron in a specific region of the brain have been observed in people with schizophrenia. Iron levels may contribute to the pathogenesis of schizophrenia in combination with other genetic, environmental and dietary factors. Iron can also contribute to the better cognitive functioning of a patient with schizophrenia, and due to frequent malnutrition and undernourishment in this group of patients, it is crucial to take into account the need for routine hematological examinations and the determination of essential nutritional deficiencies.Finally, our goals were to systematically review the literature published in the last two decades using PubMed, Web of Science, Scopus and Google Scholar. We described the clinical aspects and etiological factors of schizophrenia. We determined whether schizophrenia can be associated with iron concentration disorders to recognize and identify potential patients with iron deficiency and treat them promptly in daily clinical practice.

Determination of Essential Minerals in the Indigenous Vegetables Solanum nigrum (Stout Shade) and Gynandropsis gynandra (Spider Plant) from Two Agroecological Zones in Kisii County, Kenya.

Vegetables are good sources of essential mineral elements that promote good health and immunity. Information on the nutritional contents of indigenous vegetables is scarce. Therefore, this study sought to ascertain the concentrations of magnesium, manganese, chrome, zinc, copper, and iron in Solanum nigrum and Gynandropsis gynandra indigenous vegetables from two agroecological zones (upper midland and lower highland) of Kisii County, Kenya, using inductively coupled plasma optical emission spectroscopy (ICP‒OES). For Gnandropsis gynandra, the most abundant erythrocytic synthesis element was Fe (1856.67 ± 15.28mg/kg DW) for plants harvested from Nyanchwa (UM), and the least was Cu (8.90 ± 0.44mg/kg DW) in plants harvested from Kari (LH). In addition, Mg was the hypoglycemic element with the highest concentration (5975.00 ± 10.00mg/kg DW), and Cr lowest (3.16 ± 0.45mg/kg DW) in samples harvested from Matongo (UM). For Solanum nigrum, the most erythrocytic synthesis element was Fe (1280.00 ± 10.00mg/kg DW for samples collected from Kiamabundu (UM), and the least was Cu (9.08 ± 0.15mg/kg DW) in the samples from Nyanchwa (UM), whereas Mg in samples from Nyabioto (UM) was the hypoglycemic element with the highest concentration (4920.00 ± 10.00mg/kg DW) and Cr in samples from Mariba (LH had the lowest concentration) (3.95 ± 1.63mg/kg DW). The concentrations of elements in the two indigenous vegetables from the UM agroecological zone were slightly greater than those in the LH agroecological zone. Nonetheless, the variations observed were not statistically significant (P < 0.05). Enzymatically bio accessed concentrations of iron, zinc, chromium, magnesium, manganese, and copper were higher than those obtained aquatically. The indigenous vegetable bio avails substantial amounts of iron and copper to enable them be used in the management pernicious anaemia; on the other hand, the substantial bio availed levels of zinc, manganese, magnesium, and chromium enables the vegetable to be used in the management of diabetes.

Assessment on the Exposure of Air, Water and Noise Pollution, and Mental Stress on Preeclamptic Patients of Rajshahi, Bangladesh

Although preeclampsia claims the lives of 70,000 mothers and 500,000 newborns each year worldwide, its origin is still elusive and a number of risk factors such as environmental pollution are not yet addressed properly. In this study, exposures due to the extents of air, water and noise pollution as well as mental stress on preeclamptic patients have been investigated. Using a cross-sectional longitudinal design, 90 women hospitalized with preeclampsia in 7 hospitals of Rajshahi, Bangladesh were considered, of which Rajshahi Medical College Hospital is a tertiary referral hospital. The data were collected by interviewing the patients, physical examinations and merging the patients’ data with British Geological Survey’s groundwater data-sets (n=3,540). For statistical analyses, SPSS software was employed. It was found that most of the patients’ living rooms were within 15 feet from kitchen. Only 10% patients had good room ventilation, while the remaining 90% patients had either moderate or poor room ventilation. Combination of these facts reveals that the preeclamptic patients were subject to moderate CO2 exposure. Since 79% of the preeclamptic patients’ living rooms were below 50 ft from the nearest roads and 84% for 100 ft distance, they would experience noise pollution. Combination of traffic conditions and potential sources of noise pollution revealed that 60% of the preeclamptic patients experienced moderate to intense noise pollution. Groundwater arsenic, calcium, magnesium, iron and sodium concentrations in the patients’ drinking water were higher than WHO guideline values that should favour constipation and mild hypertension. While 70% patients were under high mental stress and 24% under very high mental stress, only 6% patients had moderate mental stress. It is concluded that air, water and noise pollution, and mental stress are potential risk factors of preeclampsia.

Clustering Methods for the Characterization of Synchrotron Radiation X‐Ray Fluorescence Images of Human Carotid Atherosclerotic Plaque

This study employs computational algorithms to automatically identify and classify features in X‐Ray fluorescence (XRF) microscopy images. Principal component analysis (PCA) and unsupervised machine learning algorithms, such as Gaussian mixture (GM) clustering, are implemented to label features on a collection of XRF maps of human atherosclerotic plaque samples. The investigation involves the hard X‐Ray nanoprobe (NanoMAX) at MAX IV synchrotron radiation facility, utilizing scanning transmission X‐Ray microscopy (STXM) and XRF techniques. The analysis covers regions of interest scanned by the beam with a step size of 200 nm, yielding XRF maps of elements like calcium, iron, and zinc. These maps reveal intricate structures unsuitable for manual labeling. However, they can be accurately classified in an automated fashion using GM. Prior to clustering, PCA is used to deal with repeated patterns and background areas. The resulting clusters are associated with different types of features, which can be identified as specific tissues confirmed by histology. Regions of high concentrations of phosphorus, sulfur, calcium, and iron are found in the samples. These regions are also observed in the STXM results as spots of low transmission that typically are associated with calcium deposits only.

Friction stir processing on a strontium modified, thin-wall, vacuum-assisted high-pressure die-cast Aural-5 alloy to improve tensile and fatigue performance

This study explores the application of friction stir processing (FSP) to enhance the material properties of Sr-modified Aural-5 alloy, with a focus on improved tensile and fatigue properties. Aural-5 is a well-known vacuum-assisted high-pressure die-cast (HPDC) Al–Si7–Mg alloy used in the automotive industry to reduce vehicle weight, enhance fuel efficiency, and lower carbon emissions. This alloy modifies its material chemistry with Sr for fine fibrous networks of eutectic silicon and manganese (Mn) to reduce die soldering. It has significantly less iron (Fe) content resulting in the elimination of detrimental needle-shaped Fe-bearing β-phase intermetallic and improving ductility. The initial microstructure of as-received HPDC Aural-5 exhibits shrinkage porosity in the middle section, a dendritic microstructure with fibrous Al–Si eutectic colonies, a shear-band structure beneath the die-wall, large dendritic externally solidified crystals (ESCs), needle-shaped Mg2Si phase and significant second-phase particulates. Some of those microstructural features, such as porosity, ESCs, needle-shaped Mg2Si phase, and large second-phase particles, serve as initiation sites for cracks under mechanical loading, resulting in adverse effects on tensile properties, particularly ductility. FSP effectively transforms the microstructure into a wrought configuration with uniform particle distribution by eliminating porosity and disintegrating dendrites, eutectic colonies, ESCs, second-phase particles, and shear-band structures. FSP-driven microstructure modification enhances yield strength and tensile ductility by ∼30 % and ∼35 %, respectively. The fatigue life of the material in a bending mode configuration (stress ratio R = 0.1) after FSP exhibits enhancements ranging from 2.0 to 3.9 times that of the original HPDC Aural-5 alloy, depending on the applied stress level.

Effect of biochar and inorganic fertilizer on the quality of beetroot (Beta vulgaris L.) in Kenya

Despite its health benefits, the production and quality of beetroot is still low in Kenya due to the application of non-recommended rates of fertiliser and soil amendment. This research aimed at contributing to the improvement of the beetroot quality in Kenya. It was designed to determine the effects of biochar and NPK (17-17-17) on the quality of beetroot in Kenya. An RCBD factorial experiment was conducted at Egerton University farm, Kenya, for two seasons. Biochar (0, 5, 10 t/ha) and NPK (0, 200, 300 and 400 kg/ha) were applied together before planting. Data were collected on beetroot diameter, total phenolics, total soluble solids, calcium, iron and phosphorus contents and analysed using SAS statistical software. The co-application of biochar and NPK significantly (p≤0.05) increased the beetroot diameter, iron, calcium, phosphorus, TSS and phenolics content in season two and not in season one. The sole application of biochar showed a significant increase in the iron content of beetroot in season one. However, biochar did not have a significant effect on beetroot diameter, mineral content, TSS and phenolics content of beetroot in season two. The sole application of NPK at 200, 300 and 400 kg/ha significantly (p≤0.05) increased the diameter of beetroot and iron content in both seasons one and two and also significantly improved the calcium and phosphorus content in season two. These NPK levels were not statistically different from each other, but different from the control. It is therefore recommended to apply NPK and biochar for quality beetroot.