Aims: This study aimed to assess Serum Vitamin D level and female teenage caries experience. Methods: This study evaluated 330 healthy female Iranian students between 13 to 19 years residing in Rey city. A questionnaire collected their demographic information. They underwent clinical dental examination to determine the number of decayed (D), missing (M) and filled (F) teeth (DMFT index). The nutritional status was evaluated using the food frequency questionnaire(FFQ) by assessing the consumption of cariostatic, cariogenic, remineralizing, sticky foods and carbohydrates. The serum samples were collected to measure the serum vitamin D level using high-performance liquid chromatography(HPLC). Data were analyzed by multiple logistic regression and independent samples t-test. Results: The mean DMFT was 5.34±3.94. Of all, 69.3% had severe vitamin D deficiency, 20.3% had moderate vitamin D deficiency and 10.4% had normal level of vitamin D. Cariostatic agents consumption had a significant inverse correlation with DMFT (P=0.006). An increase in serum level of vitamin D by more than 10.27 ng/mL was associated with a reduction in prevalence of dental caries by 22%. Increased consumption of cariostatic agents by more than 89.30 g/day decreased the prevalence of dental caries by 32%. No significant association was noted between the prevalence of dental caries and level of parent’s education, consanguinity of parents, level of income, place of residence, frequency of tooth brushing, dental flossing, and dental visits (P>0.05). Conclusions: While the serum vitamin D level had no significant effect on DMFT, the nutritional regimen seemed to play a more important role in caries control.