iPBS Markers Research Articles

Analysis of Inter-Primer Binding Site Retrotransposon Length Polymorphism in Selected Group of Vaccinium corymbosum

Abstract The genetic variability in 13 selected genotypes of Vaccinium corymbosum using re-trotransposon-based iPBS markers system was characterized in this study. The four selected iPBS primers amplified 232 fragments, average 58 fragments per primer, approximately 53.16% of which were polymorphic. The polymorphism information content of iPBS marker was 0.238. The hierarchical cluster using unweighted pair group method with arithmetic mean analysis divided selected genotypes into two main groups and several subgroups. The values of the Jaccard coefficient of genetic relatedness were between 0.036–0.243. The similarity between two sample sets was 3.6%–24.3%. This study confirmed inter-PBS amplification technique as a reproducible and trustable marker system, but has proved to be an effective DNA fingerprinting technology to generate polymorphism in Vaccinium corymbosum.

Molecular characterization of some currants (Ribes species) from Türkiye using inter-primer binding site (iPBS) and simple sequence repeat (SSR) markers

AbstractThis study was carried out to determine the genetic relationships and variation levels among currant genotypes and cultivars in Türkiye. Ten currant cultivars and one hybrid Jostaberry cultivar, which are breeding products of Europe but are best known and have high commercial value in Türkiye, were used. One black-fruited currant cultivar from Türkiye was included. In addition, 14 currant genotypes naturally grown in Türkiye were used. In this study, 14 retrotransposon-based inter-primer binding site (iPBS) and 10 microsatellite-based simple sequence repeat (SSR) markers were used for a total of 26 currant samples. By evaluating the allele profiles obtained from PCR and capillary electrophoresis with iPBS and SSR primers, the size range, average number of alleles, total number of alleles, number of polymorphic alleles, polymorphism rate, heterozygosity, polymorphism information content, marker index and discriminating power were determined successfully. In addition, unweighted pair group method with arithmetic mean (UPGMA) dendrograms and principal coordinate analysis (PCoA) were constructed and performed. The amounts of amplification product produced using iPBS and SSR markers for currant species with different fruit colours were also compared. In this study, it was proven that the level of genetic relatedness among currant cultivars and genotypes can be determined with high accuracy by iPBS markers. Moreover, the use of iPBS markers in currants was performed for the first time in this study. On the other hand, with the preferred SSR markers, the level of genetic relatedness among currants was successfully determined.

Revealing Genetic Diversity and Population Structure in Türkiye’s Wheat Germplasm Using iPBS-Retrotransposon Markers

Investigating the genetic diversity and population structure of wheat germplasm is crucial for understanding the underlying variability essential for breeding programs and germplasm preservation. This research aims to contribute novel insights with respect to the genetic makeup and relationships among these wheat genotypes, shedding light on the diversity present within the Turkish wheat germplasm. In this study, iPBS-retrotransposon markers were employed to analyze 58 wheat genotypes, encompassing 54 landraces and 4 cultivars sourced from Türkiye. These markers serve as genetic indicators that can be used to evaluate genetic variation, build genealogical trees, and comprehend evolutionary connections. The PCR products were visualized on agarose gel, and bands were scored as present/absent. The ten iPBS primers collectively yielded an average of 16.3 alleles, generating a total of 163 polymorphic bands. The number of alleles produced by individual markers ranged from 4 (iPBS-2386) to 29 (iPBS-2219). The genetic parameters were calculated using the popgen and powermarker programs. The genetic relationships and population structures were assessed using the ntsys and structure programs. Polymorphism information content (PIC) per marker varied from 0.13 (iPBS-2390) to 0.29 (iPBS-2386), with an average value of 0.22. Shannon’s information index (I) was calculated as 1.48, while the number of effective alleles (Ne) and Nei’s genetic diversity (H) were determined to be 0.26 and 0.31, respectively. Genotype numbers 3 (Triticum dicoccum) and 10 (Triticum monococcum) exhibited the maximum genetic distance of 0.1292, signifying the highest genetic disparity. Population structure analysis revealed the segregation of genotypes into three distinct subpopulations. Notably, a substantial portion of genotypes clustered within populations correlated with the wheat species. This population structure result was consistent with the categorization of genotypes based on wheat species. The comprehensive assessment revealed noteworthy insights with respect to allele distribution, polymorphism content, and population differentiation, offering valuable implications for wheat breeding strategies and germplasm conservation efforts. In addition, the iPBS markers and wheat genotypes employed in this study hold significant potential for applications in wheat breeding research and germplasm preservation.

Current State of Natural Populations of Paeonia anomala (Paeoniaceae) in East Kazakhstan

Paeonia anomala L. is a valuable and sought-after medicinal plant for treating therapeutic pathologies. The natural habitat of P. anomala in the Republic of Kazakhstan is located in the mountainous areas of the East Kazakhstan region. P. anomala is listed in the Red Book of Kazakhstan as a rare species with limited distribution. In this regard, we studied a strategy for preserving the biological diversity of P. anomala wild population. In particular, the ecological, phytocenotic, and floristic characteristics of five P. anomala populations in East Kazakhstan were explored. The anatomical, morphological, and genetic variability of the species in various habitats was evaluated. Overall, the condition of the P. anomala population in the study region can be considered satisfactory. The floristic composition of P. anomala plant communities recorded 130 species belonging to 35 families and 101 genera. The northern slopes of mountains and shrub-grass communities with leached chernozem with high contents of N-NO3 and P2O5 appeared to be optimal for P. anomala growth. Asteraceae (13%), Rosaceae (13%), Poaceae (10%), and Ranunculaceae (9%) are the major families of P. anomala flora and plant communities. The Eurasian (54%), Asian (24%), and Holarctic (15%) groups were recognized as dominant in the chronological spectrum. Amplification with iPBS primers resulted in the generation of 505 fragments, 257 of which were polymorphic. Our research results indicate that the genetic differentiation of the Kazakhstan populations is not quite as high and may indicate their long-term existence within one large population. A separate branch is formed by the P5 population, which is located separately from other populations, confirming its genetic isolation. The analysis of genetic diversity iPBS markers suggests the existence of a large, unified P. anomala population in Kazakhstan Altai.

Use of retrotransposon based iPBS markers for determination of genetic relationship among some Chestnut Cultivars (Castanea sativa Mill.) in Türkiye.

The aim of this study was to reveal the genetic relationships among some economically important chestnut cultivars for Türkiye by using retrotransposon-based inter primer binding site (iPBS) markers. In this study, a total of 19 iPBS markers were used to determine the genetic relationships among 11 chestnut cultivars (Castanea sativa Mill.). In the study, chestnut cultivars named Hacıömer, Osmanoğlu, Sarıaşlama, Erfelek, Kemer, Işıklar, Şekerci, Siyah Bursa, Tülü, Bouche De Betizac and Marigoule were the preferred cultivars utilised. Using the online marker efficiency calculator (iMEC), some indices of polymorphism, such as the mean heterozygosity, polymorphism information content, marker index and discriminating power, were determined. In addition, the size ranges of alleles, number of average alleles, number of total alleles, number of polymorphic alleles, and polymorphism rate were determined at a successful level. The chestnut cultivars of Hacıömer and Şekerci were determined to be the most similar cultivars with a similarity coefficient value of 0.924, and they formed a subgroup together with the chestnut cultivars Osmanoğlu and Erfelek, showing close similarity with these two cultivars. The use of iPBS markers in chestnuts in Türkiye was carried out for the first time in this study. The power of iPBS markers to evaluate the genetic relationship for our preferred chestnut cultivars was revealed. For this reason, it has emerged that it will be useful in the molecular characterization of both genotypes in natural chestnut populations and chestnut breeding materials such as varieties and cultivars in chestnut breeding programs.

Molecular Characterization, Population Structure Analysis, and Association Mapping of Turkish Parsley Genotypes Using iPBS Markers

Parsley (Petroselinum crispum Mill.) is a vegetable that has many benefits for human health, and its cultivation is increasing. In order to carry out breeding studies in parsley, genotypes should be characterized in terms of some characteristics. In this study, some phytochemical properties of 18 different parsley genotypes were revealed, and their genetic diversity was determined with the iPBS (Inter Primary Binding Site) marker system. In the study, the polymorphism rate was 31.9%, the mean PIC (Polymorphic Information Content) was 0.17, and the similarity coefficients were between 0.87 and 0.99. The number of subpopulations was determined as two, and 10 markers were detected at expression levels of 19–33% related to phytochemical properties. The results of this study show that there is a phytochemical and genetic variation in parsley. Parsley genotypes with certain phytochemical properties and genetic structures can be used more effectively in breeding programs.

The comparison of polymorphism among Avena species revealed by retrotransposon-based DNA markers and soluble carbohydrates in seeds

Here, we compared the polymorphism among 13 Avena species revealed by the iPBS markers and soluble carbohydrate profiles in seeds. The application of seven iPBS markers generated 83 bands, out of which 20.5% were polymorphic. No species-specific bands were scored. Shannon’s information index (I) and expected heterozygosity (He) revealed low genetic diversity, with the highest values observed for A. nuda (I = 0.099; He = 0.068). UPGMA clustering of studied Avena accessions and PCoA results showed that the polyploidy level is the main grouping criterion. High-resolution gas chromatography revealed that the studied Avena accessions share the same composition of soluble carbohydrates, but significant differences in the content of total (5.30–22.38 mg g−1 of dry weight) and particular sugars among studied samples were observed. Sucrose appeared as the most abundant sugar (mean 61.52% of total soluble carbohydrates), followed by raffinose family oligosaccharides (31.23%), myo-inositol and its galactosides (6.16%), and monosaccharides (1.09%). The pattern of interspecific variation in soluble carbohydrates, showed by PCA, was convergent to that revealed by iPBS markers. Thus, both methods appeared as a source of valuable data useful in the characterization of Avena resources or in the discussion on the evolution of this genus.

MOLECULAR DIVERSITY ANALYSIS OF HUNGARIAN APRICOT (Prunus Americana L.) VARIETIES BASED ON INTER-PRIMER BINDING SEQUENCE (iPBS) MARKERS

In this study, iPBS markers were used for assessing genetic diversity and determining the relationship among apricot cultivars. Six apricot genotypes from Hungary were examined with 11 iPBS retrotransposon markers through PCR amplification. The results show that 86 amplified bands were scored with an average of 8.6 amplicons for each primer. The number of polymorphic bands ranges from 0 to 4, averaged at 1.6. The percentage of the polymorphic band varies from 0 to 40.0%, with an average of 18.6%. The polymorphic information content ranges from 0.00 to 0.17, with a mean of 0.06. The resolving power varies from 0.00 to 1.67, with an average of 0.63. iPBS markers can differentiate only the MK135 and Veecot genotypes. The remaining Magyar kajszi apricot genotypes could not be determined by using iPBS markers.

Genetic variation of Cerastium alpinum L. from Babia Góra, a critically endangered species in Poland

Babia Góra massif is the only site of occurrence of the Cerastium alpinum L. in Poland, an arctic-alpine perennial plant with a wide distribution in North America, northwestern Asia, and Europe. To determine whether the isolated Polish populations are genetically distinct, we have performed an evaluation of C. alpinum from Babia Góra with the use of iPBS markers. A total number of 133 individuals of C. alpinum from seven populations representing four localizations of the species were analyzed, i.e., from Babia Góra (Poland), Alps (Switzerland), Nuolja massif (Sweden), and Kaffiøyra (Svalbard, Norway). Genetic analysis of all C. alpinum samples using eight PBS primers identified 262 bands, 79.4% of which were polymorphic. iPBS markers revealed low genetic diversity (average He = 0.085) and high population differentiation (FST = 0.617). AMOVA results confirmed that the majority of the genetic variation (62%) was recorded among populations. The grouping revealed by PCoA showed that C. alpinum from Svalbard is the most diverged population, C. alpinum from Switzerland and Sweden form a pair of similar populations, whereas C. alpinum from the Babia Góra form a heterogeneous group of four populations. Results of isolation by distance analysis suggested that the spatial distance is the most probable cause of the observed differentiation among populations. Although significant traces of a bottleneck effect were noted for all populations of C. alpinum from Babia Góra, the populations still maintain a low but significant level of genetic polymorphism. These results are of great importance for developing conservation strategies for this species in Poland.

Assessment of genetic diversity and population structure in local alfalfa genotypes using iPBS molecular markers

iPBS retrotransposon markers, in other words, inter-primer binding site markers based on retrotransposon, have been helpful for the determination of genetic diversity in several plants. The study was evaluated using 10 iPBS molecular markers on the level of genetic diversity and genetic structure of alfalfa genotypes. A total of 280 alleles in 50 alfalfa genotypes (48 local genotypes and 2 commercial varieties) were obtained by 267 polymorphic markers with an average of 28 per locus, ranging from 9 to 41 alleles. The rate of polymorphism of the markers ranged from 86.36 to 100% with an average of 93.71%. The average number of polymorphic bands per marker was detected as 26.7. The mean PIC value and Dice's similarity index were calculated as 0.14 and 0.50 respectively. The results of UPGMA analysis, principal coordinate analysis (PCoA), and STRUCTURE demonstrated that the 50 alfalfa genotypes could be classified into 4 subpopulations, namely the Q1, Q2, Q3, and Q4. The Nei’s genetic distances ranged from 0.0121 to 0.0359. iPBS markers and alfalfa genotypes used in this study may be used in studies of alfalfa breeding and germplasm conservation.

Exploring genetic diversity and Population structure of five Aegilops species with inter-primer binding site (iPBS) markers.

Turkey is not only a center of origin for wheat, but also contains wild forms of various cereals. Turkey, located in the Fertile Crescent, has conserved its genetic richness to the present day. The aim of the study was to investigate the genetic diversity of 70 wild wheat species, to evaluate the structure of diversity in germplasm and to generate useful data for further breeding programs. Genetic diversity and population structure of 70 wild wheat species (Ae. cylindrica, Ae. geniculata, Ae. triuncialis, T. dicocoides, Ae. columnaris) collected from Eastern and Southeastern Anatolia regions of Turkey were investigated in this study with the use of inter-primer binding site (iPBS) markers. Of 35 iPBS primers used, 11 yielded a total of 61 alleles. Number of alleles per marker varied between 2 (iPBS-2085) and 9 (iPBS-2394) with an average value of 5.55. Polymorphic information content (PIC) values varied between 0.22 and 0.47, with an average value of 0.35. Average number of effective alleles (Ne) was identified as 1.9488, Nei's genetic diversity (H) as 0,4861 and Shannon's information index (I) as 0.6791. Cluster analysis through unweighted pair-group mean average (UPGMA) method revealed that 70 wild wheats were divided into three main clusters. Genetic similarity between the genotypes, calculated with the use of NTSYS-pc software, varied between 19% (YB2 and YB70) and 98% (YB66 and YB67). Principal coordinate analysis (PCoA) revealed that three principal coordinates explained 62.33% of total variation. Moreover, population structure analysis showed that all genotypes formed three sub-populations. Expected heterozygosity values varied between 0.2666 (the first sub-population) and 0.2330 (third sub-population), with an average value of 0.2500. Average population differentiation measurement (Fst) was identified as 0.3716 for the first sub-population, 0.3930 for the second subpopulation and 0.4804 for the third sub-population. Based on present findings population structure of 70 wild wheat genotypes collected from Eastern and Southeastern Anatolia regions of Turkey were successfully characterized with the use of iPBS markers. Present findings suggested that iPBS-retrotransposon markers could reliably be used to elucidate genetic diversity of Aegilops genotypes.

Analyse of iPBS lenght polymorphism in selected group of Vitis vinifera, L varieties

Here, the specific natural variability of iPBS (Inter Primer Binding Sites Polymorphism) fingerprints in thirteen varieties of Vitis vinifera, L. was performed. All of the analysed biological material was collected in the vineyard of Sabo vinery for describing of the existing genetic polymorphism. Young leaves from a total of thirteen grapevine varieties were obtained in the in the Small Carpathians wine region of Slovakia, Vrbové. Genetic length polymorphism was studied by iPBS markers. A dendrogram of genetic similarity of generated fingerprints was constucted by UPGMA (Unweighted Pair Group Method with Arithmetic mean) and the Jaccard coefficient of genetic similarity was used for the analyse of 13 Vitis vinifera, L varieties. The generated dendrogram is separated into three major clusters at the genetic dissimilarity of 0.58. Cluster 1 is composed of two red varieties – Alibernet and Cabernet Sauvignon. Cluster 2 was further subdivided into two sub-clusters, where the larger one include all white varieties - Pinot Blanc, Müller-Thurgau, Welschriesling, Irsai Oliver, Grüner Veltliner, Pálava, Weisser Riesling, Sauvignon Blanc and Feteasca Regala. The second subcluster is comprised from two red varieties – Blaufränkisch and Dornfelder. The analysis prooved the the iPBS technique is an effective retrotransposon based markers to evaluate the variability of the genome in the germplasm of Vitis vinifera L. cultivated varieties.

IPBS-Retrotransposon Markers in the Analysis of Genetic Diversity among Common Bean (Phaseolus vulgaris L.) Germplasm from Türkiye.

Beans are legumes that play extremely important roles in human nutrition, serving as good sources of protein, vitamins, minerals, and antioxidants. In this study, we tried to elucidate the genetic diversity and population structure of 40 Turkish bean (Phaseolus vulgaris L.) local varieties and 5 commercial cultivars collected from 8 different locations in Erzurum-Ispir by using inter-primary binding site (iPBS) retrotransposon markers. For molecular characterization, the 26 most polymorphic iPBS primers were used; 52 bands per primer and 1350 bands in total were recorded. The mean polymorphism information content was 0.331. Various diversity indices, such as the mean effective allele number (0.706), mean Shannon’s information index (0.546), and gene diversity (0.361) revealed the presence of sufficient genetic diversity in the germplasm examined. Molecular analysis of variance (AMOVA) revealed that 67% of variation in bean germplasm was due to differences within populations. In addition, population structure analysis exposed all local and commercial bean varieties from five sub-populations. Expected heterozygosity values ranged between 0.1567 (the fourth sub-population) and 0.3210 (first sub-population), with an average value of 0.2103. In contrary, population differentiation measurement (Fst) was identified as 0.0062 for the first sub-population, 0.6372 for the fourth subpopulations. This is the first study to investigate the genetic diversity and population structure of bean germplasm in Erzurum-Ispir region using the iPBS-retrotransposon marker system. Overall, the current results showed that iPBS markers could be used consistently to elucidate the genetic diversity of local and commercial bean varieties and potentially be included in future studies examining diversity in a larger collection of local and commercial bean varieties from different regions.

Morphometric traits and iPBS based molecular characterizations of walnut (Juglans regia L.) genotypes

In this study, walnut genotypes that were selected during two growing seasons among thousands of seedlings were analyzed in terms of detailed morphometric, phenological, and chemical traits. A multivariate analysis was conducted with valuable traits for breeding and selection such as morphometric traits, chemical composition, and phenological characteristics. Also, genotypes were characterized by a retrotransposon-based iPBS marker system. The correlation analysis showed significant positive and negative correlations between agro-morphological characters. The principal component analysis explained 71.44% of the total variance into five main components. Principal component and hierarchical cluster analysis divided genotypes into three groups and identified subgroups based on both agro-morphological characters and iPBS marker systems. A high level of polymorphism ratio was observed for tested markers. Mantel’s test demonstrated relatively low correlations between molecular and morphological treats (r = 0.04). The genetic similarities among all individuals ranged from 0.39 (between 018 and 015 or 045 genotypes) to 0.98 (between 090 and 094 genotypes) with a mean similarity of 0.67. Remarkable phenotypic and molecular variations were observed among the genotypes. The features of some investigated genotypes were above the acceptable thresholds for walnut selection in breeding programs, and our study indicated that iPBS markers can be beneficial in walnut breeding programs, allowing the evaluation of the genetic relationship between genotypes, helping to differentiate and select the best genotypes to improve agronomic properties.

Inter-primer binding site (iPBS) retrotransposon markers provide insights into thegenetic diversity and population structure of carrots (Daucus, Apiaceae)

Studies of genetic diversity and population structure are essential as an initial step in conservation and breeding programs for modern crops. Carrot (Daucus spp.) is among the ten most important vegetables worldwide, however, its genetic structure and phylogenetic relationships are not totally deciphered. Here, we explored the utility of 21 inter-primer binding site (iPBS) retrotransposon markers to determine the genetic diversity and population structure of 38 accessions of Daucus and one accession of a related genus. The manual scoring revealed 309 bands based on their presence/absence. The dendrogram based on the UPGMA clustering algorithm and a principal coordinate analysis (PCoA) indicated the presence of four clusters. The Daucus species with 2n = 18 chromosome (subclade A´) separated from the other two species D. pusillus and D. muricatus, which were positioned into two individual clusters. The other clade includes the Daucus from the B group. It was also noticed that few accessions were intermixed amongst clusters. Different genetic diversity parameters were estimated based on the four clusters (populations) defined by STRUCTURE software, demonstrating that clusters 3 and 4 possessed the lowest and highest diversity values, respectively. AMOVA showed variation between and within clusters of 41.85% and 58.15%, respectively. The highest population divergence (Fst) was observed between clusters 2 and 3 (0.579), on the other hand, clusters 1 and 4 depicted the lowest Fst with 0.160. Our research highlighted that iPBS markers were successful and effective to study Daucus genetic diversity. These results will contribute to the genetic improvement of carrots and sustainable management of its diversity.

Using IPBS markers to study the genetic diversity of native grape varieties

Using IPBS markers to study the genetic diversity of native grape varieties

The first report about genetic diversity analysis among endemic wild rhubarb (Rheum ribes L.) populations through iPBS markers

Approximately 30% of plant species of Turkey, which is among the richest countries in terms of biodiversity, has been endemic. Wild rhubarb (Rheum ribes L.) is a wild vegetable grows especially in the eastern region of Turkey and is an endemic species. In this study, genetic relationships among 80 wild rhubarb genotypes collected from some regions of Lake Van Basin, which are in the distribution area, were tried to be determined by iPBS marker system. At the same time, a commercial variety of R. rhabarbarum, which is a cultivated species, was used as control. PCR studies were conducted with 23 iPBS primers to determine genetic relationships, and a total of 340 scorable bands were obtained. 100% polymorphism rate was obtained from all primers studied. While the average PIC value was found to be 0.90, the highest value was found to be 0.97 from the primer # 2220. It was determined that the genotypes were divided into 3 basic groups in the dendogram created with UPGMA based on Jaccard similarity coefficient.

Evaluation of the genetic variability of Ophrys insectifera L. populations growing in Belarus using molecular iPBS markers

An analysis of genetic heterogeneity was performed for two populations of Ophrys insectifera L. located in the Berezinsky Biosphere Reserve and the Verkudy village. The species are critically endangered (CR). The analysis of data obtained using iPBS markers detected the higher risk of extinction of the population in the Berezinsky Biosphere Reserve than the population from the Verkudy village, since the level of population genetic heterogeneity from the Verkudy is higher compared to the Berezinsky Biosphere Reserve. Molecular variance analysis (AMOVA) and other parameters of genetic variation showed the major fraction of intrapopulation variation (64 %, Gst = 0.26). Principal Coordinates Analysis (PCoA) revealed two groups that coincided with natural populations, and confirmed a larger genetic heterogeneity in the population from the Verkudy.

Molecular Characterization of Some Soybean (Glycine max L.) Varieties

Küresel ısınma ve insan popülasyonunun sürekli artması besin kaynağı üzerinde baskı oluşturmaktadır. Soya fazlaca protein ve yağ içermesinden dolayı insan beslenmesinde önemli rol oynamaktadır. Bu çalışmada 12 soya çeşidinin (Nova, Nazlican, SA88, Ataem7, Arısoy, A3127, Türksoy, Adasoy, Yemsoy, ANP2018, Yeşilsoy ve Samsoy) 6 IPBS markörü ile genetik ilişkisi incelenmiştir. IPBS analizi sonucuna göre toplam 44 polimorfik bant elde edilmiş olup ortalama polimorfizm oranı %85.83 olmuştur. Primer başına elde edilen ortalama polimorfik bant sayısı 7.33 olarak belirlenmiştir. Geneotipler arasında Dice benzerlik indeksi 0.1 ile 0.9091 arasında değişmiş olup ortalama benzerlik indeksi 0.4506 olarak hesaplanmıştır. Analizlerin sonuçlarına göre, en yakın akrabalık gösteren genotiplerin Yeşilsoy ile Arısoy çeşitleri iken, en az benzeyen genotiplerin ise Samsoy ile Yemsoy çeşitleridir. Sonuç olarak, IPBS markörleri soya ıslahı programlarında uygun ebeveynlerin seçiminde genotipleri karakterize etmek için etkili bir şekilde kullanılabilir.

GENOMIC FINGERPRINTS OF ARACHIS HYPOGAEA L. NATURAL GERMPLASM AS REVEALED BY iPBS MARKERS

Arachis hypogaea, L. is an oil seed crop with a worldwide importance and the seeds are eaten at one of several stages from immature to fully ripe, raw, or cooked. The aim of the study was to analyze specific iPBS fingerprints of twenty-one accessions of peanut that were collected in the places of their natural occurrence in Bolivia and describe the existing genetic polymorphism. For genomic imprinting, three different iPBS markers were chosen - 1882, 2079, 2274 and a PCR reactions were performed. Obtained iPBS fingerprints were evaluated for the presence/absence of individual amplified loci and scored in 1/0 matrices. A Jaccard coefficient of genetic similarity was applied in UPGMA analysis for dendrogram construction. Polymorphism level was achieved in the range from 48% up to the 75% per primer. None of the iPBS markers used in the study was considered to distinguish all of the analyzed peanut accessions, but combining them in the final analysis, the level of genomic polymorphism was sufficient to clear separating of iPBS fingerprints of the collected accessions and unique iPBS loci were recorded in genomes of some of them. We found that, by selecting the appropriate iPBS markers, it is possible to characterize the peanut genome in the individual level with a specific fingerprint.