Abstract
Arachis hypogaea, L. is an oil seed crop with a worldwide importance and the seeds are eaten at one of several stages from immature to fully ripe, raw, or cooked. The aim of the study was to analyze specific iPBS fingerprints of twenty-one accessions of peanut that were collected in the places of their natural occurrence in Bolivia and describe the existing genetic polymorphism. For genomic imprinting, three different iPBS markers were chosen - 1882, 2079, 2274 and a PCR reactions were performed. Obtained iPBS fingerprints were evaluated for the presence/absence of individual amplified loci and scored in 1/0 matrices. A Jaccard coefficient of genetic similarity was applied in UPGMA analysis for dendrogram construction. Polymorphism level was achieved in the range from 48% up to the 75% per primer. None of the iPBS markers used in the study was considered to distinguish all of the analyzed peanut accessions, but combining them in the final analysis, the level of genomic polymorphism was sufficient to clear separating of iPBS fingerprints of the collected accessions and unique iPBS loci were recorded in genomes of some of them. We found that, by selecting the appropriate iPBS markers, it is possible to characterize the peanut genome in the individual level with a specific fingerprint.
Highlights
Arachis hypogaea, L. is a world known oil seed crop that is harvested mainly in semi-arid tropical, subtropical and temperate regions (Naidu et al, 1999; Proite et al, 2007)
By selecting the appropriate iPBS markers, it is possible to characterize the peanut genome in the individual level with a specific fingerprint
Inter primer binding sites polymorphism was analyzed in the study for the peanut accessions
Summary
L. is a world known oil seed crop that is harvested mainly in semi-arid tropical, subtropical and temperate regions (Naidu et al, 1999; Proite et al, 2007). The diversity of uses of peanuts is an evidence of its antiquity - the seeds are eaten at one of several stages from immature to fully ripe, raw, or cooked. They are processing by boiling, broiling, roasting, crushing or grounding and mixing with other food. Arachis hypogaea is an allotetraploid species (2n = 4x = 40, AABB) with a very large and complex genome. Cytological, it behaves mostly as a diploid, but multivalents can result in skewed genetic ratios and likely account for many of the “off types” (Leal-Bertioli et al, 2015).
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