IPA Analysis Research Articles

P143 The effect of JAK inhibition on the metabolome of neutrophils from patients with rheumatoid arthritis

Abstract Background/Aims Janus kinase inhibitors (JAKi) including baricitinib and tofacitinib target the JAK/STAT pathway and are clinically effective in treating rheumatoid arthritis (RA). Neutrophils play a key role in the pathophysiology of RA and their function is directly affected by these drugs as shown by clinical trials of JAKi which report increased infection rates and transient neutropenia during therapy. The aim of this work is to determine difference in the metabolome of neutrophils from healthy controls and patients with RA, and the effect of JAKi on the metabolome of healthy and RA neutrophils. Methods Neutrophils were isolated from healthy controls (HC) (n = 10) and patients with RA (n = 10 DMARD-naïve, n = 10 Biologics-naive) and pre-incubated with baricitinib, tofacitinib or a pan-JAK inhibitor (all 200ng/mL) for 2h before snap-freezing. Metabolites were extracted by 50:50 v/v AcN:H2O and analysed by 1H NMR spectroscopy using a 700 MHz Avance IIIHD Bruker NMR spectrometer equipped with a TCI cryoprobe. Chenomx, Bruker TopSpin and tameNMR software were used to identify metabolites and process spectra. Statistical analysis and machine learning models were implemented using R and the Mixomics package. Pathway analysis was carried out using Ingenuity (IPA). ROS production in response to 15 min fMLP stimulation was measured using DHR-123 and flow cytometry. Results At 0h, changes in levels of NADP+, NADPH and hypoxanthine in RA neutrophils indicated increased production of ROS via NADPH oxidase and xanthine oxidase compared to HC. Lower levels of glucose and increased levels of lactic acid also suggested increased glycolysis in RA neutrophils compared to HC. However after 2h incubation with JAKi, a difference in the response to these drugs was observed. 80 metabolites were higher in RA neutrophils compared to HC following 2h JAKi treatment. IPA analysis revealed the metabolites higher in RA neutrophils were involved in NAD biosynthesis, NAD phosphorylation/dephosphorylation, amino acid synthesis and transport, and carbohydrate metabolism. Changes in the levels of NADP+, NADPH and hypoxanthine in RA neutrophils indicate that JAKi inhibit ROS production. We therefore confirmed experimentally that JAKi inhibit ROS production in response to fMLP (p < 0.05). IPA also predicted altered neutrophil cell functions including cell death and cell-to-cell signalling. We previously showed that JAKi did not affect the constitutive rate of neutrophil apoptosis, however JAKi significantly inhibit the delay in apoptosis induced by GM-CSF (p < 0.05). No significant difference in the metabolome was observed when comparing tofacinib-treatment and baricitinib-treatment in either the HC or RA patient group. Conclusion The metabolome of RA and HC neutrophils is different at baseline. JAKi alter the levels of intracellular metabolites involved in neutrophil activation and ROS production in RA. This may explain, in part, the efficacy of JAKi in decreasing disease activity and joint damage in RA. Disclosure M. Fresneda Alarcon: None. R. Grosman: None. S. Chokesuwattanaskul: None. M. Phelan: None. H. Wright: None.

전통적 IPA와 수정된 IPA를 이용한 항공서비스학과의 온라인 교육서비스 품질에 관한 연구

This study seeks to examine online education that has replaced face-to-face classes and has become the center of university education, not the role of assisting face-to-face classes since Corona 19. To this end, the importance of online education service quality and the resulting satisfaction were examined through IPA analysis using the 253 part survey of aviation service students in Gwangju, Jeollanam-do, Jeonbuk, and Chungnam. The verification results of this study are as follows: First, a quadrant of high importance and high satisfaction was both important and satisfactory for professors' posture and expertise in traditional IPA analysis, and the revised IPA resulted in high importance and satisfaction of technical support and evaluation processes. Second, the two quadrants, which are of high importance but low satisfaction, are located only in the case of traditional IPA analysis, and were items of access to the education system, communication and information on employment. For the revised IPA, the same item is located in four quadrants, with two IPAs producing completely different results with the same item. Third, two IPA analyses were located in the three quadrants with low importance and satisfaction, and the items were found to be convenient to take, diversity in lecture content, and appropriateness of content. After conducting the traditional IPA, the revised IPA was to identify the difference between the two IPA analyses and to increase students' satisfaction with the quality of online education services based on the results, suggesting effective educational services in aviation services.

Perceptions of pre-service early childhood teachers about special education coursework through an IPA analysis

이 연구는 특수교육학개론 교과 운영 개선을 위한 정보를 구할 목적으로 예비유아교사를 대상으로 특수교육학개론 교과에 대한 인식을 알아보았다. 이를 위해 특수교육학개론을 수강한 유아교육전공 대학생 70명을 대상으로, 특수교육학개론 교과에 대한 중요도와 만족도를 조사하였다. 조사 문항은 총 12문항으로, 2개 하위 영역으로 구성되어 있다. 분석의 방법은 IPA매트릭스 분석을 이용하였으며, 평균점수를 기준으로 분석하였다. 연구 결과, 예비유아교사들은 특수교육학개론 15주차 수업 내용 중 ‘통합교육 현장실습’이 가장 중요하다고 인식하였으며, ‘개별화 교육계획’에 대한 만족도가 가장 높게 나타났다. IPA 매트릭스 분석결과, 중요도와 만족도가 모두 높은 영역에는 ‘개별화 교육계획’이 분포하였고, 높은 중요도와 낮은 만족도를 나타내는 영역에는 ‘통합교육 현장실습, 낮은 중요도와 낮은 만족도를 나타내는 영역에는 ‘특수교육 연계 서비스에 대한 이해’, 중요도는 낮으나 만족도가 높은 영역에는 ‘장애의 유형과 특성’이 분포하였다. 이러한 연구결과를 토대로, 대학에서의 특수교육 수업 운영 방향에 대해 제언을 하였다.

An International Case Study Supporting Basic Proficiency: Focusing on Ireland and Canada

An International Case Study Supporting Basic Proficiency: Focusing on Ireland and Canada

Step-Counting Function of Adolescent Physical Training APP Based on Artificial Intelligence

With the rapid development of the information age, Internet and other technologies have been making progress, people’s fitness awareness has been gradually enhanced, and sports fitness app has emerged as the times require. This paper mainly studies the step-counting function of physical training app for teenagers based on artificial intelligence. This paper uses the modular development method to achieve the functional requirements of the system as the goal, respectively, for parameter management, website configuration, system log, interface security settings, SMS configuration, WeChat template message and several functional modules to achieve system configuration. In this paper, three types of sensors are used to analyze the data changes in the process of walking through three types of data, and different weights are given as the results of step-counting. When the peak value of sensor data is measured, only the peak value of the primary axial data of each sensor is analyzed, which should be determined according to the actual axial value of the sensor. In this paper, the users’ evaluation indexes of sports fitness app are divided into two groups: importance and satisfaction, so the obtained data are directly divided into two groups: importance and satisfaction of user experience indexes of sports fitness app, and the two groups of data are matched with the sample t test to ensure the scientific conclusion. Finally, the advantages and disadvantages of the user experience of college students’ sports fitness app are analyzed through IPA analysis. Heuristic evaluation is carried out on the step app to score the second-level usability index of the app. The first-level usability index score and the total usability score of the step app are obtained by calculation. There is not much difference between male and female students who use sports apps. Among them, 288 are male students, accounting for 58.2% of the total and 16.4% are female students. The results show that the use of artificial intelligence technology can reduce the overall energy consumption of step-counting algorithm, so as to achieve an energy-saving step-counting algorithm.

IPA 분석을 활용한 초등학교 교사의 과정중심평가 지원 요소 탐색

IPA 분석을 활용한 초등학교 교사의 과정중심평가 지원 요소 탐색

High-throughput global transcriptional profiling to identify the STAT3 signaling pathway as a potential biomarker for immune checkpoint inhibitor resistance in metastatic/advanced urothelial carcinoma.

474 Background: Advanced/metastatic urothelial carcinoma (UC) is a significant public health burden with median overall survival of 15 months. Although, immune checkpoint inhibitors (ICI) have provided an additional second line treatment option, only 15-40% of patients will respond. There has been much effort in determining the mechanisms of immunotherapy resistance and predictive biomarkers to further improve these treatments. Methods: Pre-treatment genomic sequencing data derived from FFPE samples from the IMVIGOR210 clinical trial (n=298) was accessed for analysis. Briefly it was a single arm phase II clinical trial where advanced/metastatic UC patients refractory to platinum chemotherapy treatment received the ICI atezolizumab. This study has been published with detailed methods (PMID: 28950298). The raw sequencing data was pre-processed using standard QC measures and aligned to the human reference genome (hg38). The resulting outputs were then normalized and processed to generate the gene level counts for differential gene expression (DGE). We did DGE analysis comparing patients who had clinical benefit (CR, PR, SD) vs non-clinical benefit (ie. PD) to atezolizumab. The list of differentially expressed genes were then analyzed using various gene ontology, pathway and systems biology tools (IPA, Enrichr, and X2Kweb). Further subset analysis was done using gene-gene correlations (ie. PD-L1 and STAT3) and clinicopathologic features (eg. gender, race, smoking history). Results: Among the 298 patients in this study, there were 25 with CR, 43 with PR, 63 with SD, and 167 with PD based on clinical response to atezolizumab. Subgroup analysis for CR vs PD patients found that approximately 847 genes were differentially expressed with statistical significance (p ≤ 0.05). IPA analysis for this list of differentially expressed genes found among the top signaling pathways were “primary immunodeficiency” and “sirtuin signaling”. Further subset analysis of 39 genes (p ≤ 0.01) enriched in PD patients using Enrichr and X2kweb found that there was an overrepresentation of STAT3 signaling genes (hypergeometric p-val 6.32x104). Conclusions: Our results found that when the transcriptional profiles of CR vs PD there was differential gene expression in STAT3, primary immunodeficiency, and sirtuin signaling pathways. Of note it has been reported that STAT3 signaling can modulate immune activity and its expression is correlated with poor prognosis in urothelial carcinoma patients. These results warrant a larger study to see if STAT3 signaling is a potential biomarker for ICI resistance. If validated this may indicate that the STAT3 pathway is a potential therapeutic target to overcome ICI resistance and improve the efficacy of these agents.

A Study on the Gap Between Knowing the Importance of and the Actual Implementation of Family-centered Practices of Social Workers at Community Welfare Centers in Gyeonggi-do: Focused on IPA Analysis

A Study on the Gap Between Knowing the Importance of and the Actual Implementation of Family-centered Practices of Social Workers at Community Welfare Centers in Gyeonggi-do: Focused on IPA Analysis

USABILITY APLIKASI ANDROID HONDA e-Care DENGAN USE QUESTIONNAIRE DAN IMPORTANCE PERFORMANCE ANALYSIS (IPA)

Honda Pondok Pinang, which is located on Jl. Ciputat Raya No.80 Pondok Pinang, South Jakarta, in customer service using an android application called HONDA e-Care. HONDA e-Care is very helpful in making it easier for consumers to access Honda service services. Customers can book services at Honda dealers online, get notifications of periodic maintenance schedules, and search for the nearest dealer from a location that can be seen on a digital map by first accessing the Honda e-Care application. But consumers often also face problems, such as when booking a service, but the ongoing booking service does not appear. Until now, no one has conducted research on the quality of the Honda e-Care application. This study aims to measure the extent of the usability of the HONDA e-Care android application and provide suggestions for developing the HONDA e-Care application in the future. Based on the gap analysis, it was found that in general the HONDA e-Care android application on the Honda Pondok Pinang was not satisfied with the quality of the current applications. Based on the IPA analysis, the priority scale for improving the quality of the HONDA e-Care android application can be mapped where quadrant I is the highest priority scale according to the user's perspective, namely: [2], [5], [7], [8] and [9]. These four items must be upgraded immediately by the manager to meet user expectations.

Rheumatoid Arthritis Synovial Fluid Neutrophils Drive Inflammation Through Production of Chemokines, Reactive Oxygen Species, and Neutrophil Extracellular Traps.

Rheumatoid arthritis (RA) is a chronic inflammatory disorder affecting synovial joints. Neutrophils are believed to play an important role in both the initiation and progression of RA, and large numbers of activated neutrophils are found within both synovial fluid (SF) and synovial tissue from RA joints. In this study we analyzed paired blood and SF neutrophils from patients with severe, active RA (DAS28>5.1, n=3) using RNA-seq. 772 genes were significantly different between blood and SF neutrophils. IPA analysis predicted that SF neutrophils had increased expression of chemokines and ROS production, delayed apoptosis, and activation of signaling cascades regulating the production of NETs. This activated phenotype was confirmed experimentally by incubating healthy control neutrophils in cell-free RA SF, which was able to delay apoptosis and induce ROS production in both unprimed and TNFα primed neutrophils (p<0.05). RA SF significantly increased neutrophil migration through 3μM transwell chambers (p<0.05) and also increased production of NETs by healthy control neutrophils (p<0.001), including exposure of myeloperoxidase (MPO) and citrullinated histone-H3-positive DNA NETs. IPA analysis predicted NET production was mediated by signaling networks including AKT, RAF1, SRC, and NF-κB. Our results expand the understanding of the molecular changes that take place in the neutrophil transcriptome during migration into inflamed joints in RA, and the altered phenotype in RA SF neutrophils. Specifically, RA SF neutrophils lose their migratory properties, residing within the joint to generate signals that promote joint damage, as well as inflammation via recruitment and activation of both innate and adaptive immune cells. We propose that this activated SF neutrophil phenotype contributes to the chronic inflammation and progressive damage to cartilage and bone observed in patients with RA.

Health-oriented optimization strategies of coastal fitness environment in Qingdao

Under the initiative of “co-construction and sharing, health for all”, the natural condition of coastal area in Qingdao becomes the key area of the national fitness. Based on the health-oriented theory, authors analyze the health-oriented connotation of the coastal fitness environment, and suggest that the realization of health-oriented coastal fitness environment needs to meet the spatial demand of health-oriented behaviors and health behaviors. These behaviors should maintain accessibility and friendliness. Based on the present status survey and IPA analysis, it is concluded that the continuity and gradual changes are in urgent need of improvement, followed by smoothness, then convenience and recognizability need to update. Therefore, in combination with the acceptable time and distance, authors put forward specific optimization strategies from four aspects. That is, coastal fitness environment in Qingdao can be improved by network of coastal footpaths, intelligent management of color lightings, reasonable connecting of road materials and humanized and recognizable fitness site, in order to promote residents’ health activities and national fitness.

Architectural practice education and space utilization plan in the post-covid 19 era-in case of chungbuk national university department of architecture

As COVID 19 spreads rapidly around the world in December 2019, social discussions are actively underway in architecture and urban fields to respond to a changed society, and educational facilities are responding to the spread of sensitivity through space use and movement control. In order to conservatively control infectious diseases, controlling the use of physical space was the most reliable method, but the strength and limitations of non-face-to-face classes began to become clear in architecture education due to physical restrictions for one year. For the efficient use of architectural educational spaces in the post-COVID 19 era, it is necessary to prepare a plan for use according to the social distancing stage. Therefore, this study aims to clarify the areas suitable for online space and the areas in which physical spaces should be used in architectural education, and then derive a plan that can effectively utilize the physical education space according to the social distancing stage. Interviews and surveys were conducted with 99 students and instructors in the Department of Architecture at Chungbuk National University, and through IPA analysis, it was intended to derive problems and utilization plans for architectural education after the COVID 19. As a result of the study, the problem of communication was one of the biggest problems in the practical education, and in the use of the practice space, the satisfaction of the process requiring physical space was the lowest. Therefore, the computer-related architectural practice education can be converted into an online space and used, and the existing computer room is divided into partitions to form a space dedicated to design class critique. In addition, three desk arrangement plans were suggested to respond to the spread of COVID 19 while focusing on communication through the rearrangement of the desks in the design room. This study is limited to the practice space of the Department of Architecture, Chungbuk National University, and the research is limited to students and instructors. It will be a study. © 2021 Architectural Institute of Korea.

중요도-만족도 분석을 활용한 경관보전직불제 개선방안

중요도-만족도 분석을 활용한 경관보전직불제 개선방안

수정 IPA 기법을 이용한 호텔 서비스 회복 품질 연구 : 객실 서비스를 중심으로

수정 IPA 기법을 이용한 호텔 서비스 회복 품질 연구 : 객실 서비스를 중심으로

IPA를 활용한 여가활동으로 유소년 축구클럽 선택속성에 관한 연구

IPA를 활용한 여가활동으로 유소년 축구클럽 선택속성에 관한 연구

A Study on Global Supply Chain Risk Management Strategies Under COVID 19

기업의 경영활동이 글로벌화되고 적시생산체제(JIT)로 운영되면서 공급사슬이 복잡해졌으며, 이로 인해 다양하고 심각한 위험에 노출되고 있다. 위험관리를 어떻게 수행하느냐는 위험의 발생을 억제할 뿐만 아니라 이로 인한 피해를 최소화하는데 중요한 역할을 하게 된다. 따라서 위험요인에 대한 분석과 전략방안을 규명하는 것은 기업의 경쟁력에 핵심요소가 될 것이다. 본 연구는 공급사슬위험의 분야별 위험요인의 중요도를 도출하고 요인별 선호 전략과의 관계를 규명하는 데 목적이 있다. 이를 위한 연구방법은 계층분석(AHP)방법과 중요도-성과(IPA) 분석법을 활용하였다. 분석결과 공급사슬위험요인의 분야로 수요와 공급부문의 위험요인이 가장 중요한 것으로 나타났다. 위험요인에 대한 경감전략에서 기업들은 수요의 변동성, 법률과 정책변화, 각종재해 등과 같은 기업이 통제하기 어려운 분야는 수용전략을 활용하는 반면 통제가 가능한 요소인 재고부족, IT시스템 장애 등은 감소전략을, 그리고 상대기업의 문제에서 비롯된 요인에 대하여는 전가전략을 활용하는 것으로 나타났다.Purpose : This study aims to identify risk factors for each sector of the supply chain the relationship between risk factors and preferred strategies. Research design, data, methodology : IBM SPSS 25.0 was used for demographic analysis of questionnaire data, descriptive statistics analysis and IPA analysis were conducted to compare the importance and preferences of the businesses and expert groups. Further, AHP analysis was conducted to understand the relative risk importance of factors for mitigating supply chain risk by employing the Expert Choice 11 statistical program. Results : The risk factors in the supply and demand sectors are crucial. In the mitigation strategy to reduce risk, it was found that companies follow acceptance strategies in areas that are difficult to control, such as volatility in demand, changes in laws and policies, and natural disasters. On the contrary, companies tend to use a reduction strategy for controllable factors such as inventory shortages and IT system failures, and imputation strategies for factors arising from problems with business partners. Conclusions : Companies show a high tendency to use risk mitigation strategies considering the possibilities and interests that they can control for various supply chain risks. This suggests that companies eventually prefer or use strategies that are consistent with the ultimate purpose of the business.

PENILAIAN PELANGGAN MENURUT PERSEPSI KEPUASAN DAN KEPENTINGAN PADA FASILITAS RUANG PUBLIK STASIUN KERTAPATI DAN TANJUNG KARANG

Kertapati (KPT) and Tanjung Karang(TNK) central stations serve as passangers’ arrival, departure points and goods handling facilities. Those facilities should be supported by public service facilities the needs of the users. One method the measure the needs of the users is servedon user’s survey on. On user’s survey on perception on needs. The research measures the indeks of the user’s perception on needs and satisfaction at two stasions, analysis the gap between satisfaction, needs and ranks the priority of satisfaction and need. The research carry out based on a qualitative descriptive research method in which a Servqual Analysis was utilities measure the users’perspective subsequanley an IPA Analysis are utilited to measure the priority important. Parameters were based on minimum standard level (SPM No.48 Tahun 2015) from Ministry of Transportation that were Safety, Security, Reliability, Comfort, Accessibility and Equality. The respondents was choosen based on accidental sampling whose already experienced and journey with trains. The results are beneficial to get insight about the sustainable improvement as well as to determaine the priority of improvement needed. Result from the stations are good service with several variables being the priority of improvement. Access to disability with gap of -1.13 for KPT and -1.02 for TNK. The stairs go up and down to the train with gap of -1.12 for KPT. The public toilets with gap of -0.93 for KPT and -0.98 for TNK. Disabled facilities with gap of -1.09 for TNK. Toilet for disabled people with a gap of -1.07 for TNK.Keywords: Perception, Satisfaction, Importance, Servqual, IPA

Parent and Teacher Understandings of the Needs of Autistic Children and the Processes of Communication between the Home and School Contexts

Introduction: Autism is a spectrum condition with symptom presentation varying widely. Teachers and parents face challenges in supporting autistic children. There are similarities in how teachers and parents make sense of autism, but there may be differences in priorities and approach potentially leading to misunderstandings. Where parents are actively engaged in their child’s school life the impact on outcomes is positive. SAFE with Schools is a new intervention in development designed to support parents and teachers of autistic children to build collaborative relationships. The study aims to explore understandings among parents and teachers, areas of difference and agreement and perceptions of communication between the two contexts. Methods: Teacher–parent units (N=32) for 13 autistic children, were recruited. The parents and teachers completed reflective journals and carried out semi-structured interviews focusing on the aims of the study. A blended thematic interpretive approach drawing from IPA and Thematic Analysis methodological approaches was used to analyse the resulting data. The analysis proceeded by extracting themes for the parents and the teachers separately. Subsequently these were compared, to identify which themes appeared for both and how they were employed in similar or divergent ways. Findings: The following 6 themes emerged from the data: Each Child is Unique, Behavioral Differences between Home and School, Building Positive Relationships as Essential to Child’s Wellbeing, Emotional Impacts of the Autism, Bureaucracy as a Barrier and Feelings of Control. Although these themes were shared by teachers and parents there were differences in emphasis and expression in practice between the two contexts. Discussion: The findings related to various bodies of both clinical and educational literature which highlight the need for interventions such as SAFE with Schools that take a systemic, attachment-based approach to facilitating strong relationships with and around the autistic child.

Study on tourists' satisfaction with night tourism in Wuhan city based on revised IPA analysis method

Study on tourists' satisfaction with night tourism in Wuhan city based on revised IPA analysis method

Abstract 12683: Genome-wide Mrna Expression Analysis of Acute Psychological Stress Responses

Introduction: Previous studies have examined effects of acute psychological stress in humans on selected panels of genes. This exploratory study aimed to investigate genome-wide transcriptional activity changes in responses to acute psychological stress. Methods: The sample included 40 healthy women who participated in a previous study (22 who had a stress induced experience (experimental group) and 18 who did not (control group)). The psychological stress was induced by the Trier Social Stress Test (TSST). Psychological stress levels (measured by the Subjective Units of Distress Scale (SUDS) and the state anxiety subscale of the Spielberger State-Trait Anxiety Inventory) and hemodynamic changes (measured by blood pressure (BP) and heart rate (HR)) were assessed before and after the TSST. The peripheral blood samples obtained before and after the TSST were processed for mRNA-sequencing. Results: The mean age of the participants was 31 years (SD 11.6). The psychological and hemodynamic stress parameters indicated that the TSST successfully induced moderate but statistically significant levels of acute psychological stress in the participants of the experimental group. Compared with the participants in the control group, six genes ( BCL2L14, FAM3B, HCG26, HLA-F-AS1, LOC101928710 , and SLC22A16 ) were up-regulated and 9 genes ( ATP2C2, CA1, CRYBG3, FBXO9, HBD, SLC39A9, SNCA, STRADB , and TRMT12 ) were down-regulated among those who experienced stress induction. The IPA analysis identified one network of cell-to-cell signaling and interaction, cellular function and maintenance, and hematological system development and function. Thirteen genes out of 15 were found in this network. Conclusions: The identified genes including FAM3B, BCL2L14, SLC22A16 , and SNCA , have been proposed as the therapeutic targets of diabetes-related CVDs, certain cancers, and Parkinson’s disease, but have not previously been reported to be associated with psychological stress. Future studies are suggested to examine the pathological mechanisms by which the identified genes may mediate the association between psychological stress and adverse health outcome.