Investigation of transport phenomena in the nanoscopic channels (pores) having the characteristic cross dimension of less than 100 nm is of utmost importance for diverse areas of applied biology, medicine and technology. One of the mainstream lines of development of nanofluidic systems and applications today is creation of biosensors capable of sensing single molecules and manipulating them in a controllable manner. Molecules can be detected based on the measurements of ionic currents through appropriately sized channels: entry into a channel of a molecule with the effective cross-dimension comparable to that of the channel lumen is accompanied by decrease of the ionic current recorded at a given transmembrane potential. The transport properties of such channels can be modulated by coating their walls with lipid bilayers, two-dimensional fluids capable of sustaining transport processes within them. In our present work, we made use of this property of the membranes to develop a method for detecting and controllably transporting single-stranded DNA molecules through channels formed by lipid membrane cylinders with the luminal radius of 5-7 nm. Entry of a DNA molecule into such a channel in the conditions of low (∼10 mM) ion strength proved to be accompanied by detectable increase of its ionic conductivity in a manner dependent on the direction of the electric field gradient. The amplitude of the conductivity increment can be credibly used to quantify the number the DNA molecules within the channel. Besides that, adsorption of DNA molecules on the lipid bilayer surface was shown to render the membrane cylinder the properties of a voltage-dependent channel with ion selectivity. The work was financially supported by the Russian Science Fund No 17-75-30064.