Metal nanowires are a 1D structure possessing distinct properties suitable for diverse applications. Copper nanowires (Cu NW) have garnered attention in recent times, particularly as an antibacterial material. Nevertheless, their utilization as a bandage or dressing material necessitates a suitable support, an aspect that remains largely unexplored. In this study, we have developed activated carbon fiber (ACF)-supported Cu NW using the chemical reduction and wet impregnation methods. Tested against Escherichia coli and Staphylococcus aureus, Cu NW/ACF exhibits 83—86% reduction in the bacterial growth. The improved antibacterial activity of the material is attributed to the slow release of Cu ions from Cu NW. Biocompatibility test confirms less than 1.5% hemolysis of erythrocytes, indicating the potential application of the composite as a bandage or dressing material.