Ion-exchange Chromatographic Analysis Research Articles

Studies on the Nitrogen-Phosphorus Compounds. VII. Ion Exchange Chromatographic Analysis of Condensed Phosphate Mixtures

イオン交換クロマトグラフィーを縮合リン酸塩の分析に実用化できるような条件を究明した。1)低縮合リン酸塩の混合物中におけるオルト,ピロ,トリポリリン酸塩まで,溶離液の濃度をかえずにそれぞれの成分に分離し,市販トリポリリン酸ナトリウム,ピロリン酸カリウムの分析を簡単にした。2)テトラポリリン酸塩を含む鎖状,環状低縮合リン酸塩の分離はむずかしかったが,分別溶離法でオルト,ピロ,トリポリ,テトラポリ,トリメタ,テトラメタリン酸塩を完全に分離することができた。この方法は2次元ペーパークロマトグラフ法と比較しても容易に行なうことができ,しかも精度がよく,五酸化リンの低温加水分解生成物の分析に適用できる。3)従来のグラジエンド エリュウションクロマトグラフ法にくらべ,樹脂,溶離液の使用を少量にとどめ,重合度1～10程度の縮合リン酸を迅速に分析できるようにした。

Automation of Ion Exchange Chromatographic Analysis of Condensed Phosphate Mixtures

Automation of Ion Exchange Chromatographic Analysis of Condensed Phosphate Mixtures

BIOCHEMISTRY OF THE EYE

NO DETAILED analysis of the free amino acid content of the vitreous body has been reported. Duke-Elder 1 reported a total value, and Krause 2 determined the basic amino acids of the vitrein and mucoid portions of the vitreous. More recently Schaeffer and Murray 3 recorded the results of microbiological assays of the whole vitreous protein. In view of the paucity of detailed knowledge of the amino acids in the vitreous and their role in the chemical structure of that body, this report presents results of ion exchange chromatographic analysis of the amino acids which occur free in the vitreous humor. MATERIALS AND METHODS Bovine eyes were obtained from a meat-packing plant. Within four to eight hours after removal of the orbs from the freshly killed animal, the vitreous was separated and filtered by gravity. The filtered liquid was stored for short periods at 4 C. and in the frozen