The objectives of the present study were to determine the iodine concentration in milk sampled from 80 commercial dairy farms located in eastern (n=58) and southwestern (n=22) Ontario, and to identify if the iodide content of groundwater consumed by the lactating herd, along with other factors, are associated with higher bulk milk iodine (BMI). A bilingual questionnaire addressing water consumption, nutrition, milking management practices, and well characteristics was completed by each producer. The 127I concentration in milk and groundwater samples was established using inductively coupled plasma mass spectrometry. Potential predictor variables were screened for univariable significance (p0.05), and a general linear regression model was fitted to assess associations between BMI and explanatory variables such as 127I in water, well depth and age, water treatment, the use of iodine-based pre or post-dips, application strategies and post-dip coverage goal. Results of the data analysis suggest that there is a strong positive correlation between the iodide content of groundwater and BMI levels (p0.001). Post-milking practices including the use of an iodine-based teat disinfectant, the application strategies of a post-milking teat disinfectant and overall coverage goal of the solution on teats were also significantly (p0.05) associated with increased BMI levels. These results suggest a significant association exists between BMI levels in relation to post-milking management practices and the iodine content in groundwater consumed by the lactating herd. Post-milking practices and drinking water containing excessive levels of iodine should be monitored frequently and where needed mitigation strategies implemented to prevent high BMI levels on farm.