Iodine Acetate Research Articles

Iodine Acetate as a Mild Selective Agent for the Wagner–Meerwein Rearrangement in 3a,6-Epoxyisoindoles

The iodine-initiated cationic skeletal Wagner–Meerwein rearrangement in tetrahydro-3a,6-epoxyisoindol-1-ones has been studied. It was shown that by the action of iodine acetate in acetic anhydride the reaction proceeds regio- and stereoselectively with the formation of 5-iodo-4,6-epoxycyclopenta[c]pyridin-4-yl acetates. The proposed reagent provides better yields of rearrangement products than those described in the literature.

Specific effector cell (EC) immunomodulation improves the repair function of adipose mesenchymal stroma cells (aMSC) on an experimental model of grade3-4 osteoarthrosis (OA). Comparation with other current therapies

Background & Aim Different cellular therapies for OA knee cartilage repair are limited to grade 1 and 2 lesions. These methods producefibrous cartilage (instead of hyaline) because itsextra cellular matrix (ECM) is impoverished in collagen 2 and enrichedin collagen 1. The small thickness and poor subchondral bone integration make this repaired tissue fragile. To overcome these ECM defects a new therapeutic approach associating the use of tissue specific EC, cocultured withsyngeneicaMSCis presented here. Theapproachwas tested on an animal model of G3-4 OA. Its efficacy was compared with other current therapies Methods, Results & Conclusion Methods 44Wistar rats (250 g)were intra articular (IA) injected with a0.2% solution of mono iodine acetate (MIA, MERK Lab). After 7 days, 4 animals were euthanized and injected joins, as well its contralateral, were collected for 0-day histology controls. The rest of animals were divided into 5 groups of 8 animals each. Group 1 was kept w/o treatment as a Control Group,Group 2 IA treated with syngeneic aMSC, Group 3 with syngeneic activated EC, Group 4 with a co-culture of EC+aMSC and Group 5 with syngeneic PRP. 4 animals of each group were euthanized at day 7, and the remain 4 rats were euthanized for day 14. aMSC were obtained from subdermal, collagenase dissociated, fat tissue. EC, obtained from mechanical dissociatedsplenic cells and 96 hours challenge of hyaline cartilage lysate in RPMI Media culture.EC+aMSCafter 18 hoursco-culture. PRP from blood of complete cardiac bleeding. Histologic exam was done through H&E and Alcian Blue Stains, and IHC stains for Collagens Type 1, 2, and 10, aggrecans, and CD 45+ cells. Results Histologic preparation of control group rats shows, since day 0 through day 14, the effectiveness of MIA IA treatmentproducing Grade 3-4. Almost complete cartilage disappearance, fibrotic scar formation in between both join surfaces, and strong inflammatory reaction at subchondral bone and synovia tissues.All the tested treatments controlled the inflammatory reaction. aMSC treated rats developed fibrous cartilage,and fibrotic scar. EC induces poor hyaline cartilage formation. EC+aMSC co-culture induces almost complete recovery of cartilage,subchondral bone and synovium tissue. PRP, after a transient cartilage protection, induces sever scar formation in between both join surfaces. Conclusion IA injection of EC+aMSC co-culture seems to be effective to repair Grade 3-4 OA hyaline cartilage on this animal model.

Injection in temporomandibular joint of rats. Description of technical protocol.

The development of animal models for research has been very diffused. Osteoarthritis is a joint degenerative pathology that induces cartilage erosion, chondrocyte proliferation and osteophyte formation. The aim of this paper is to present a technical procedure to perform the injection of monosodium iodine acetate in the temporomandibular joints of rats to generate osteoarthritis and to contribute to future research analysis related to pathology progression and proper treatment performance. The use of rat models may be a complex process because of their size, but they can be compared to the human temporomandibular joint due to the similar characteristics and the possibility of performing diagnosis and treatment protocols in order to detect this pathology.

Утворення глікогену в клітинах Chlorobium limicola за умов порушення функціонування окремих ланок циклу Арнона

Nitrates are not utilized by photosynthetic green sulfur bacteria Chlorobium limicola as the nitrogen source. Addition of these compounds into the medium inhibits the growth of bacteria but stimulates the glycogen accumulation. Nitrates are rapidly converted by the cells of C. limicola to the nitrites. Maximal nitrate reductase activity in the cells of C. limicola was detected after one day cultivation. Nitrates and nitrites inhibit activity of fumarase – the key enzyme of the reductive tricarboxylic acid cycle. Nitrites have more potential action as compared to the nitrate. The glycogen content in the cells of C. limicola cultivated in the medium with nitrates, but not acetate and pyruvate, show no significant change. Addition of acetate and pyruvate to the medium stimulate the glycogen synthesis in the presence of nitrate. Iodine acetate decreases the glycogen level under these conditions. Keywords: Chlorobium limicola , glycogen, Arnon’ cycle, nitrates.

The effect of papaverine and iodine acetate on myocardial creatinkinase

The effect of papaverine and iodine acetate on myocardial creatinkinase

Iodination of aromatic compounds with a mixture of peroxyacetic acid and iodine

A method for the iodination of water-insoluble aromatic compounds in a homogeneous system is reported; an acetic acid solution of peroxyacetic acid is introduced into a solution of iodine and excess aromatic compound. The yields of iodobenzene and iodotoluenes were 60·5 and 84·6%, respectively. Iodic acid is formed simultaneously, and is the main product in the absence of aromatic compounds. A kinetic study comparing the rate equation and rate constants for the iodination of benzene with those for the reaction of iodine with peroxyacetic under similar conditions has been made. The acidity of the solution has no appreciable effect on the rate and the data suggest a mech- anism which involves a rate-determining formation of iodine acetate or hypoiodous acid followed by a rapid condensation with benzene to form iodobenzene.