Invert Sugar Research Articles

Cunninghamella echinulata PA3S12MM invertase: Biochemical characterization of a promiscuous enzyme.

The Cunninghamella echinulata PA3S12MM fungus is a great producer of invertases in a growth medium supplemented by apple peels. The enzyme was purified 4.5 times after two chromatographic processes, and it presented a relative molecular mass of 89.2kDa. The invertase reached maximum activity at pH of 6 and at 60°C, in addition to presenting stability in alkaline pH and thermal activation at 50°C. The enzymatic activity increased in the presence of Mn2+ and dithiothreitol (DTT), while Cu2+ and Z2+ ions inhibited it. Also, DTT showed to protect enzymatic activity. The apparent values for Km , Vmáx , and Kcat for the sucrose hydrolysis were, respectively, 173.8mmol/L, 908.7mmol/Lmin-1 , and 1,388.79s-1 . The carbohydrate content was of 83.13%. The invertase presented hydrolytic activity over different types of glycosidic bonds, such as α1↔2β (sucrose), α1→4 (polygalacturonic acid), α1→4 and α1→2 (pectin), and α1↔1 (trehalose), indicating that the enzyme is multifunctional. Thus, the biochemical properties showed by the C. echinulata PA3S12MM suggest a broad industrial application, such as in the biomass hydrolysis or in the food industry. PRACTICAL APPLICATIONS: Invertases are hydrolytic enzymes employed in several industrial sectors. Given their great importance for the economy and several industrial sectors, there is a growing interest in microorganisms producing this enzyme. The analysis of the biochemical properties of invertase in C. echinulata PA3S12MM suggest applications in the food industry. Due to its increased hydrolytic activity, the hydrolysis process of the sucrose may employ invertase for the production of invert sugar. The stability at alkaline pH suggests an application in the development of enzymatic electrodes for the quantification of sucrose in food and beverage. The multifunctional activity may work in the biomass hydrolysis or saccharification of by-products for the extraction of fermentable sugars. The high level of invertase N-linked glycosylation of invertase grants this enzyme thermal stability at high temperatures, in addition to resistance against the action of proteases, which are desirable characteristics for the application of this enzyme in industrial processes.

Gummy candies production with natural sugar source: Effect of molasses types and gelatin ratios.

Nowadays, attention has been dedicated towards the development of foodstuffs whose constituents are from natural sources. In this study, grape, mulberry, and carob molasses containing natural sugars were evaluated in order to replace the use of sugar syrup and artificial additives in the production of gummy candy which was prepared by varied gelatin ratios. The molasses which have similar °Brix values (78-79) presented different types and amounts of sugar components. High invert sugar with low sucrose was observed in grape and mulberry but high sucrose with low fructose and glucose appeared in carob molasses. Unlike grape and mulberry, carob based jellies had a whitish appearance possibly as a result of the crystallization due to its high sucrose/invert sugar ratio. For all parameters of TPA, carob candy with 5 g/100 g gelatin and grape candy with 10 g/100g gelatin had the lowest and highest values, respectively. Gelatin concentration dependency trend of hardness, gumminess, chewiness and resilience was determined as grape > mulberry > carob. No significant effect (p > 0.05) on gelling temperatures (21-29°C) but significant effect (p < 0.05) on melting points (33-39°C) of molasses types were observed for candies. The properties of grape based candy having higher thermal stability and lower temperature sensitivity were attributed to its higher total sugar contents than other samples. The sensorial acceptability score for molasses gummy candies obtained more than 3.0 on a 5-point hedonic scale. These results illustrate the potential for the use of molasses in a healthier confectionery product development instead of commonly used sugars.

Processing deteriorated beet. Part 1: Indicative and quantitative analysis of the degree of deterioration

For purpose of process control and measures to handle deteriorated beet material it is of the utmost importance to monitor the beet quality parameters indicating beet deterioration. This paper presents an overview of the most appropriate methods to be applied: visual inspection of beet material, analysis of marker components like mannitol and invert sugar, and the analysis of dextran. Critical levels of these components in sugar beet are mentioned, beyond which actions in process operation should be undertaken. Recognizing deteriorating beet quality at an early stage is possible by attention to sudden or gradual changes of particular process parameters and performance indicators, which will be briefly explained.

Erzincan Geleneksel Dut Pekmezinin Bazı Fiziksel, Kimyasal ve Antioksidan Özelliklerinin Belirlenmesi

Bu araştırmada, geleneksel yöntemle üretilen Erzincan dut pekmezinin bazı kalite özelliklerinin belirlenmesi hedeflenmiştir. Bu amaçla, 2018 ve 2019 yıllarında geleneksel olarak üretilen 10 adet dut pekmezi örneğinde, çeşitli fiziksel ve kimyasal özellikler ile antioksidan aktivite tespit edilmiştir. Pekmez örneklerinde; pH 4,86-5,17, titrasyon asitliği %0,53-0,85, toplam kuru madde %71,91-84,07, suda çözünür kuru madde %70,60-83,80, toplam kül %1,44-3,47, protein %1,47-3,19, su aktivitesi 0,59-0,75, 5-hydroxymethyl furfural (HMF) 5,70-72,62 mg/kg, toplam şeker %64,04-71,40, invert şeker %40,46-56,15, sakkaroz %7,76-26,59, kalori 262,01-294,54 kcal/100 g, viskozite 745-15275 cP, L* değeri 19,19-32,64, a* değeri 5,68-17,53, b* değeri 2,46-9,01, H° değeri 15,78-52,10 ve C* değeri 8,74-19,72 aralığında belirlenmiştir. Toplam fenolik madde (TFM), 1,1diphenyl-2picyrlhydrazyl (DPPH) antioksidan aktivite ve % inhibisyon değerleri ise sırasıyla; 3,92-10,05 mg GAE/g, 1,31-6,25 mg TE/g ve %7,96-30,69 aralığında bulunmuştur. Araştırma sonuçları değerlendirildiğinde; genel olarak pekmez örneklerinin besin değerlerinin yüksek olduğu, antioksidan ve TFM açısından iyi birer kaynak oldukları ve HMF miktarlarının TS 12001 Pekmez Standardı‘nda bildirilen limitlerin oldukça altında kaldığı görülmektedir. Sonuç olarak, Erzincan yöresine ait geleneksel üretim tekniği ile doğal, besin değeri yüksek ve sağlığa yararlı pekmezlerin üretilebildiği ortaya çıkmıştır. Bu araştırma sonuçlarının, Erzincan geleneksel dut pekmezinin önemi, tanıtımı, değer kazanması ve koruma altına alınmasında katkı sağlayacağı düşünülmektedir.

Formation of structure of disperse systems in non-equilibrium state under conditions of combination of two types of cavitation effects

The description of the existing method for producing invert syrup in stages in a large volume under the conditions of using one type of energy flow is given. The purpose of this work is to develop the principles of transition from macrostatic equilibrium to the development of each microstatic volume of liquid dispersed systems in the process of their production in a non-equilibrium state created by the combination of two types of cavitation effects: hydrodynamic and acoustic effects. The properties of dispersed systems are described and the object of research is invert syrup with the amount of dry 80% and reducing 79-80% substances. Invert syrup with 100% inversion of sucrose to glucose and fructose is widely used in the production of flour confectionery and a number of pastille and marmalade products. The principles of formation of aggregates from sucrose molecules at the stage of sugar dissolution, the formation of voids and the nucleation of gas phase bubbles are established. The conditions for creating a non-equilibrium state of syrup under the conditions of using two types of energy flows, by combining hydrodynamic and acoustic cavitation, are considered. The character of the transformation of the state of bubbles under conditions of changing the flow geometry of gas-liquid systems and the excitation of sound vibrations in them is shown. The mechanism of structure formation of a gas-liquid system under the influence of acoustic vibrations on the structure is described. The character of transformation of highly concentrated gas-liquid systems after the collapse of gas phase bubbles is shown, which was the determining factor in the formation of new types of materials. The practical output of this work was the creation of invert syrup with 100% fructose and glucose content in dry substances. Preparation of an emulsion for flour confectionery products and marmalade based on fresh fruits and vegetables. The effectiveness of combining two types of cavitation exposure opens up the prospect of creating new types of confectionery products with fundamentally new properties, while preserving native vitamins and nutrients.

The use of cavitation in the technology of confectionery semi-finished products based on fruit and vegetable raw materials

One of the directions of development of innovative confectionery products is the creation of products using raw materials with critical significant substances to improve public health. The creation of confectionery semi-finished products based on fresh vegetables and fruits with a minimum temperature effect to ensure the preservation of native micronutrients are an urgent task of enriching confectionery. Cavitation is a method of physical influence in the production of confectionery and semi-finished products, which helps to increase the preservation of vitamins by reducing the duration of temperature exposure and achieving the desired properties of the semi-finished product. The aim of the study was to study the influence of cavitation effects on the structural, mechanical and physicochemical indicators of semi-finished products from fresh vegetables for the production of confectionery. Model samples of a semi-finished product are made from carrots using acoustic exposure with various durations (from 0 to 6 minutes) using invert syrup obtained by cavitation treatment. The obtained samples were compared with a control sample of a semi-finished product from carrots with the addition of invert syrup without cavitation treatment. The use of cavitation effects at the stage of obtaining invert syrup and processing carrot mass made it possible to increase the strength of the confectionery semi-finished product made on the basis of carrot raw materials by 19% to 640 g / cm3. The initial water activity of the control sample was 0.852, which indicates a high risk of microbiological changes during storage. Cavitation treatment contributed to a minimal change in the water activity of the semi-finished carrot-based product during storage. With an increase in the duration of the cavitation treatment of the carrot mass, the amount of reducing substances increased in the samples of carrot semi-finished product, in the control sample - 6.3%, in the sample from 4 and 6 minutes. cavitation treatment - 44.1% and 47.3%, respectively. A study of a semi-finished product by gas chromatography with a mass spectrometric detector revealed a significant effect of cavitation effects on the chemical composition. A decrease in the content of fumaric acid esters in the sample with the duration of cavitation effects of 4 and 6 minutes was revealed from 2.91% to 1.72% and 1.2%, respectively, compared with the control sample. At the same time, the organoleptic characteristics of the taste and smell of the semi-finished product have improved.

Effect of Type of Sugar and Dough Relaxation Time on the Shape Characteristics of Biscuits

This study was aimed to evaluate the effect of type of sugars namely dextrose, sucrose, sucrose-invert syrup mix, invert syrup and honey, and dough relaxation time of 15, 40, and 65 min. on the shape characteristics viz. width, thickness, spread ratio, and an expansion ratio of biscuits. The type of sugars significantly affected (p&lt;0.05) all shape characteristics, while the dough relaxation only affected those of crystal sugars (sucrose and dextrose). High dough relaxation time enhanced the width of sucrose biscuits without significantly affecting (p&gt;0.05) their thickness. In contrast, the thickness of dextrose biscuits decreased with increasing relaxation time. Liquid sugars (invert syrup and honey) enhanced the width of biscuits; the former enhancing the thickness as well. The study also demonstrated that a high spread ratio may also be associated with a low expansion ratio. Hence, both the spread ratio and expansion ratio must be considered when discussing the shape characteristics of biscuits with different ingredients.

Penapisan Enzim Invertase dari Khamir Asal Nektar dan Madu Hutan

Yeast is unicellular eukaryotic microorganism and one of the main resources in of organic compound. The natural compounds produced by yeasts have high commercial value; one of the examples is enzyme. Enzymes are widely used in industrial production processes. The invertase enzyme has the ability to catalyze sucrose, and hydrolyze it into equimolar monosaccharides, D-glucose and D-fructose mixtures called invert sugar. Invertase itself is commonly found in various parts of plants wich has high carbon content. Honey and nectar are compounds that have high carbon contents. Very high glucose and fructose contents show high levels of sucrose hydrolysis, so it is possible to have high levels of invertase enzymes in honey and nectar. This study aims to perform screening of invertase enzyme from yeasts that isolated from the nectar and forest honey and to find out the optimum temperature and pH for the enzyme production. This research was conducted by isolating yeast contained in wild honey and flowers nectar. Then conducted by qualitative test with selective medium and benedict test method. Quantitative test of intervase unit activity measured by DNS method with OD 540 nm. Protein concentration measured by Lowry Method with OD 650 nm. From 47 isolats 16 isolates able for invertase enzymes production. Yeast isolat SG 3.2 unit activity is 0.222 U/ml and specific activity is 7.07 U/mg. Optimum production pH and temperature are 5 and 25°C, respectively.

Detection of the cavitation component in the total increase of reducing sugars as a result of inversion of sucrose under ultrasonic influence on vegetable and fruit purees

The article presents the results of a study of the process of inversion of sucrose in apple, carrot and pumpkin puree, occurring under ultrasonic action on them, with the formation of the total amount of reducing sugars, as well as with the identification of the autonomously manifested cavitation component that occurs simultaneously with thermal inversion. The relevance of research consists in identifying the quantitative value of reducing sugars formed due to a separately flowing cavitation component in the general inversion of sucrose, when exposed to ultrasound with specified parameters, in order to obtain new types of products with different physicochemical parameters and organoleptic characteristics, depending on changes in parameters processing. To identify the part of reducing sugars formed by the cavitation effect alone, in the total inversion of sucrose occurring during ultrasonic exposure to the product, it is necessary to exclude part of the increase in reducing sugars from the total amount of increase detected by thermal exposure. As a result of the calculations, the following values of the increase in the amount of reducing sugars (relative to the original data), obtained only due to the cavitation effect during ultrasonic processing of puree, were obtained. In natural apple puree the increase was 240 mg per 100 g, and in puree with added sucrose – 495 mg per 100 g, which is 2 times higher. In carrot puree the natural increase was 74 mg per 100 g, and in puree with sucrose it was 150 mg per 100 g, which is twice higher and confirms the same pattern as in apple puree. In natural pumpkin puree the increase was 27 mg per 100 g, and in puree with sucrose it was 84 mg per 100 g, which is more than 3 times higher than in natural. The results obtained allow us to conclude that when processing the product with ultrasound, the process of sucrose inversion is intensified; including its component obtained due to the cavitation effect, and the inversion is more deeply manifested in puree with added sucrose.

Investigation of physicochemical and organoleptic properties of low-calorie functional quince jam using pectin, quince seed gum and enzymatic invert sugar

Investigation of physicochemical and organoleptic properties of low-calorie functional quince jam using pectin, quince seed gum and enzymatic invert sugar

Study of the autonomous process of inversion of sugarose through thermal influence at ultrasonic processing of vegetable and fruit pures

Relevance. The article presents the results of a study of the increase in the amount of reducing sugars in the inversion of sucrose due only to thermal effects during ultrasonic processing of vegetable and fruit monocomponent purees (with or without added sucrose). The relevance of the study is to identify the quantitative thermal component of a separate process in the total inversion of sucrose at the specified parameters of ultrasonic action on the product.Material and methods. to perform this task, experimental samples of carrot, pumpkin and Apple puree with the addition of sucrose in an amount of 5% (by weight) and without it were previously made, which were then subjected to heat treatment at the same temperature and time exposure (at a temperature of 50 °C for 30 minutes). To study the dependence of the degree of sucrose inversion on the component composition of the processed product, the mass fraction of reducing sugars – fructose and glucosewas determined in the manufactured experimental samples of natural fruit and vegetable purees (without added sucrose) and in mixtures of purees with sucrose (according to a given recipe) before and after heat treatment at the specified parameters, depending on the recipe composition and pH of the medium.Results. The obtained results of the study of the carbohydrate composition before and after thermal exposure show that the amount of reducing sugars in products subjected to heat treatment has significantly increased, compared to the initial content. Thus, in Apple puree with added sucrose, the mass fraction of fructose after heat treatment of the mixture increased by 197 mg per 100 g, i.e. by 3.8%, and glucose – by 192 mg per 100 g, i.e. by 6.7%. The total invert sugar content of the Apple mixture increased by 4.8 %. In the carrot mixture of puree with sugar, the content of total reducing sugars after thermal exposure increased by 5.4%. At the same time, in carrot natural puree, the increase in reducing sugars was only 3.6%, which is 1.5 times lower. In the pumpkin puree mixture with sugar, the total content of reducing sugars increased by 3.9%, and in the natural puree, the amount of invert sugars increased by only 2.7%, which is 1.4 times lower than puree with added sugar.

Kinetic/thermodynamic study of immobilized β-fructofuranosidase from Aspergillus tamarii URM4634 in chitosan beads and application on invert sugar production in packed bed reactor

β-fructofuranosidase (FFase) from Aspergillus tamarii URM4634 was immobilized covalently in chitosan beads. It was characterized biochemically, studied in terms of kinetic and thermodynamic parameters, and applied on conversion of sucrose for invert sugar production in a packed bed reactor (PBR). The optimum reactional conditions were determined and obtained at pH 5.0 and 60 °C. FFase was thermostable at 50–55°C. At 50°C, the enzyme shows longer half-life (t1/2) (594.13 min) and a higher D-value (1,973.64 min). This indicates that immobilized FFase was stable at temperature commonly used in invert sugar production. The following thermodynamic parameters were obtained: activation energy (E*d = 301.57 kJ mol−1), enthalpy (298.76 ≤ ΔH*d ≤ 298.89 kJ mol−1), entropy (579.88 ≤ ΔS*d ≤ 589.27 J K−1 mol−1) and Gibbs free energy (100.29 ≤ ΔG*d ≤ 108.47 kJ mol−1). The high E*d, ΔH*d and ΔG*d values confirmed FFase thermostability. The high and positive values for ΔS*d indicate an increase in disorder due opening of the enzyme structure. The sucrose hydrolysis in PBR showed a maximum invert sugar yield (96.0%) at 15 min of operation. The hydrolysis process remained efficient up to 100 min (70.22%). The results obtained in the present study provide a good indication that immobilized FFase on chitosan beads in PBR is efficient to invert sugar production for food industry.

Sugar hydrolysis and colour formation in processing

The stability of the sucrose molecule throughout processing is of the utmost importance, because in principle any sugar loss will increase the production costs per tonne of the obtained white sugar. Colour in processing is an important technological parameter too, since only an adequate colour control in processing allows the cost-effective production of white sugar crystals of the required quality. For process control of both sugar hydrolysis and colour formation it is necessary to understand the effect of pH value, temperature and retention time on these phenomena. This paper will describe the possible causes of sugar hydrolysis and colour, as well as how to control pH values and temperature in processing in order to limit the technological impact of both reactions in which invert sugar plays a central role.

The role of alternative sugars on endurance capacity in balb/c mice.

Alternative sugars containing isomaltulose were investigated to confirm the hypothesis that isomaltulose ingestion affects endurance capacity due to slow rates of hydrolysis and absorption rate at the intestine. A swimming time of the control group tends to decrease, but the group administrated with low glycemic index (GI) sweeteners tend to increase gradually. Fructo-oligosaccharide (FOS) and inverted sugar (IS), contained isomaltulose, groups showed a significant difference of change in blood glucose and lactic acid level than control group (p<.05). Serum creatine phosphokinase (CPK) and serum lactate dehydrogenases (LDH) of PAL100 were significantly lower than that of the control group (p<.05). IS showed a significant difference in glutathione peroxidase (GSH-Px) compared to Con and PAL20 (20% isomaltulose) group (p<.05). Consuming FOS seems to increase an endurance capacity since fructose and FOS based in isomaltulose contained syrup showed low absorption rate and GI level. PRACTICAL APPLICATIONS: Sugar is a major energy source for exercise, but it causes excessive intake because of the short duration of sweetness, which causes diseases such as obesity, diabetes, and skin aging. An alternative sugar complex containing isomatulose was found to be a sugar substitute for athletic performance. Athletic performance is not just for athletes or active people. In general, elderly people with low muscle mass have low mobility. Alternative sugars can be a good source of supplements to help them perform smoothly with less intake. Therefore, an endurance test with alternative sugars is and important study for energy supplements industry.

Integrative transcriptomics reveals genotypic impact on sugar beet storability

Key messageAn integrative comparative transcriptomic approach on six sugar beet varieties showing different amount of sucrose loss during storage revealed genotype-specific main driver genes and pathways characterizing storability.Sugar beet is next to sugar cane one of the most important sugar crops accounting for about 15% of the sucrose produced worldwide. Since its processing is increasingly centralized, storage of beet roots over an extended time has become necessary. Sucrose loss during storage is a major concern for the sugar industry because the accumulation of invert sugar and byproducts severely affect sucrose manufacturing. This loss is mainly due to ongoing respiration, but changes in cell wall composition and pathogen infestation also contribute. While some varieties can cope better during storage, the underlying molecular mechanisms are currently undiscovered. We applied integrative transcriptomics on six varieties exhibiting different levels of sucrose loss during storage. Already prior to storage, well storable varieties were characterized by a higher number of parenchyma cells, a smaller cell area, and a thinner periderm. Supporting these findings, transcriptomics identified changes in genes involved in cell wall modifications. After 13 weeks of storage, over 900 differentially expressed genes were detected between well and badly storable varieties, mainly in the category of defense response but also in carbohydrate metabolism and the phenylpropanoid pathway. These findings were confirmed by gene co-expression network analysis where hub genes were identified as main drivers of invert sugar accumulation and sucrose loss. Our data provide insight into transcriptional changes in sugar beet roots during storage resulting in the characterization of key pathways and hub genes that might be further used as markers to improve pathogen resistance and storage properties.

Effect of ultrasonic treatment on sucrose inversion in vegetable and fruit purees

Relevance.This article presents the results of a study of the process of sucrose inversion in vegetable and fruit monocomponent purees with and without sucrose under the influence of ultrasonic action on them.Methods.For the study, experimental samples of carrot, pumpkin and Apple puree of two types were made – a monocomponent natural puree (without sugar) and puree with the addition of 5% sucrose (by weight). The main part of the study was to study the depth of sucrose inversion in experimental samples, depending on the specified parameters of ultrasound exposure at a frequency of 21.6 kHz for 30 minutes on a laboratory cavitation unit in a flow-through cyclic mode.Results.It was found that in all experimental samples, the amount of total reducing sugars increased significantly compared to the values obtained in control samples that were not subjected to ultrasonic treatment. So, in natural apple puree (without adding sucrose) after ultrasound exposure, an increase in total reducing sugars was detected by 9.3%, and in experimental samples of apple puree with added sucrose – by 10.9%, in relation to control samples, which is 1.2 times higher than in natural puree. In samples of carrot puree (without adding sucrose), the increase in total reducing sugars was 6.7%, and in puree with added sucrose, the increase was 12.2%, which is 1.8 times higher than the inversion in puree without sugar. In samples of pumpkin puree without sugar, the increase in reducing sugars was 4.2%, and in samples with added sucrose – 9.9%, i.e. 2.4 times higher than in samples without sugar.These data allow us to conclude that ultrasound exposure can intensify the inversion of sucrose with an increase in the number of reducing sugars, and this process takes place more deeply in products containing added sucrose.

Potential Ameliorative Effects of Chromium Supplementation on Glucose Metabolism, Obesity, and Genomic Stability in Prediabetic Rat Model.

Chromium (III) (Cr(III)) effect on improving glucose, body mass loss, and genomic stability has been extensively studied in models of type 2 diabetes. However, there is a lack of studies evaluating its effect on prediabetes. Thus, this study evaluates the effects of Cr(III) as dietetic supplementation on glucose metabolism, obesity, and genomic stability on prediabetic rat model using high-invert sugar. Male Wistar rats were divided randomly into four treatment groups: (1) control, receiving standard diet (control); (2) prediabetic (PD), receiving a 32% of invert sugar; (3) Cr(III), receiving chromium (III) chloride (CrCl3•6H2O) (58.4mg/L); and (4) Cr(III) + PD, receiving CrCl3•6H2O in combination with high-invert sugar. Cr(III) supplementation significantly reduced blood glucose (123.00 ± 8.29mg/dL vs. 115.30 ± 9.31mg/dL, p = 0.015) and partially reduced area under the 120-min blood glucose response curve (AUC) in PD rats (p = 0.227). Moreover, Cr(III) attenuated weight gain (187.29 ± 38.56g vs. 167.22 ± 29.30g, p = 0.004), significantly reducing body mass index (0.68 ± 0.04g/cm2 vs. 0.63 ± 0.04g/cm2, p < 0.001), Lee index (0.30 ± 0.01 vs. 0.28 ± 0.01, p < 0.001), and peritoneal fat (p < 0.001). Regarding genomic stability, high-invert sugar, Cr(III), or the combination of both did not produce changes in oxidative stress, DNA damage in pancreas, or cytotoxicity markers. These data suggest that Cr(III) supplementation improved partially glucose metabolism and reduced obesity in rat model PD due to high-invert sugar without influence in genomic stability.

Storability as a varietal characteristic of sugar beet? – Results 2016–2018

The storability of sugar beet varieties grown in Germany has not been specified to date. In 2016–2018, nine storage trials were carried out in order to test the storability of 13 current varieties. The beet were stored in climate containers for approximately 480 °Cd. Root mass and the contents of sugar, invert sugar and marc were determined before and after storage. During storage, the sugar content decreased in all varieties while the invert sugar content increased. The observed sugar loss was most significant (about 10% of the amount before storage) in an environment where the syndrome „basses richesses“ had occurred. Differences among varieties were significant in six environments, but not for the mean of the eight trials without diseases. Variety recommendations concerning the storability of sugar beet are thus currently not possible.

Modification of Conventional Sugar Juice Evaporation Process for Increasing Energy Efficiency and Decreasing Sucrose Inversion Loss

The evaporation process, boiler, and turbine are the main components of the cogeneration system of the sugar factory. In the conventional process, the evaporator requires extracted steam from the turbine, and bled vapor from the evaporator is supplied to the juice heater and the pan stage. The evaporation process may be modified by using extracted steam for the heating duty in the pan stage. This paper is aimed at the investigation of the effects of this process modification. Mathematical models of the conventional and modified processes were developed for this purpose. It was found that, under the conditions that the total evaporator area is 13,000 m2, and the inlet juice flow rate is 125 kg/s, the optimum modified evaporation process requires extracted steam at a pressure of 157.0 kPa. Under the condition that the fuel consumption rate is 21 kg/s, the cogeneration system that uses the optimum modified evaporation process yields 2.3% more power output than the cogeneration system that uses a non-optimum conventional cogeneration process. Furthermore, sugar inversion loss of the optimum modified process is found to be 63% lower than that of the non-optimum conventional process.

Determination of Primary Metabolites, Vitamins and Minerals in Black Mulberry (Morus nigra) Berries Depending on Altitude

This research was conducted to examine the effect of altitude on primary metabolites (soluble solids, total sugars, invert sugars, individual invert sugars (glucose, fructose, sucrose), total acidity, pH, organic acids (citric acid, malic acid), vitamins (C, A, B1, B2, B3) and mineral contents (K, P, Ca, Mg, Na, Fe, Cu, Zn, Mo)) in black mulberry (Morus nigra) berry extracts. The experiment was performed at three locations: Location 1—(187 m altitude), Location 2—(271 m altitude) and Location 3—(493 m altitude). High performance liquid chromatography (HPLC) was employed for the identification of individual invert sugars, organic acids and vitamins (A, B1, B2, B3) in berry extracts. Vitamin C was evaluated by spectrophotometry, whereas the mineral contents were determined by flame atomic absorption spectrophotometry. The contents of primary metabolites, vitamins and minerals varied widely depending on altitude. The synthesis and accumulation of the tested parameters changed with increasing altitude. The highest levels of sugars were found in Location 3, whereas the values of acids, vitamin A and the minerals Na, Mo and Fe were higher in Location 1. The accumulation of macronutrients K and P, and vitamins C and B3 was most intensive in Location 2. Altitude had no effect on the contents of vitamin B1 and B2, and the micronutrients Cu and Zn. The results suggest that black mulberry fruits are rich in primary metabolites, vitamins and minerals, and that altitude has an important effect on the synthesis of the tested parameters in the fruits.