Simple SummaryWe assessed food preferences in three adult male zoo-housed gorillas over a period of 20 months. We used paired-choice tasks on a computer touchscreen and we also presented food pairings by hand using PVC tubes and compared the stability of preferences across methods and seasons. In addition, we assessed whether preferences were stable across offerings within a session. To further validate the use of images on touchscreens to assess food preferences, we showed that these gorillas chose images of highly preferred food items when these images were paired with images of their less preferred foods. These tests can help us understand the stability of animals’ preferences and whether preferences are dependent on the larger test context.Decisions about which foods to use during training and enrichment for captive animals may be based on invalid assumptions about individuals’ preferences. It is important to assess the stability of food preferences given that one-time preferences are often used to inform which items are offered over a longer period of time. Presenting preference assessments using images of food items allows control over factors such as size, scent, and inadvertent cueing but requires validation. We presented three male gorillas with choices between randomly selected pairs of actual food items from their morning meal using PVC feeders. We also presented the gorillas with two-alternative forced-choice tests between images of these foods on a touchscreen computer. Ranked preferences were correlated across method and seasons. Furthermore, gorillas selected images of preferred over less preferred foods in a validation task on the touchscreen. However, selections of some food items changed within sessions, suggesting that preference may be relative to other contextual factors. Researchers should assess how choices affect subsequent preferences to understand whether animals demonstrate absolute preferences for particular food items, or prefer variety.