Intraosseous Defects Research Articles

Injectable Platelet Rich Fibrin With Demineralized Freeze-dried Bone Allograft in Treatment of Intraosseous Defects

Injectable Platelet Rich Fibrin With Demineralized Freeze-dried Bone Allograft in Treatment of Intraosseous Defects

Evaluation of the Efficacy of a 1:1 Mixture of β-TCP and rhPDGF-BB in the Surgical Management of Two- and Three-Wall Intraosseous Defects: A Prospective Clinical Trial.

The aim of this split-mouth clinical and radiographic study was to evaluate and compare the regenerative potential of a combination of recombinant human platelet-derived growth factor-BB (rhPDGF-BB) and beta tricalcium phosphate (β-TCP) to the established technique of bone grafting with β-TCP alone in the surgical management of intraosseous defects. A total of 24 sites in 12 subjects with intraosseous defects were included and randomly divided into test (rhPDGF-BB + β-TCP) and control (β-TCP alone) sites. Clinical parameters were recorded at baseline, 3 months, and 6 months. Radiographic parameters were evaluated at baseline and 6 months. Both groups showed statistically significant reductions of all clinical parameters. Intergroup comparison demonstrated a significantly greater probing pocket depth reduction and clinical attachment level gain in the test group. The mean percentage defect fill was significantly greater in the test group than in the control group at 3 and 6 months, and greater improvement in defect angle was also evident in the test group. Although both groups showed definitive improvements in all parameters, the test group showed significantly better results when used to treat human periodontal intraosseous defects.

Evaluation of the clinical efficacy of 1.2% atorvastatin in the treatment of periodontal intraosseous defects by CBCT: A randomized controlled clinical trial.

Background. Atorvastatin (ATV), which belongs to the statin class of drugs, is the formidable inhibitor of 3-hydroxy-2- methyl-glutaryl coenzyme A reductase. This clinical trial evaluated and compared the clinical and radiographic changes in chronic periodontitis (CP) patients, obtained through 1.2% ATV as an adjunct to scaling and root planing (SRP) in the treatment of intraosseous defects. Methods. Twenty CP patients, with a minimum of one pair of bilateral intraosseous, were randomly selected for this splitmouth study. Group 1 included 20 sites treated with SRP and subgingival delivery of a placebo gel, whereas an equal number of sites in group 2 were treated by SRP along with subgingival delivery of 1.2% ATV gel. The plaque index (PI), modified sulcus bleeding index (mSBI), probing pocket depth (PPD) and clinical attachment level (CAL) were evaluated at baseline and 3- and 6-month intervals, while the intraosseous defect was assessed at baseline and 6-month interval using cone-beam computed tomography (CBCT). Paired t-test was used to determine statistical significance. Results. A greater reduction in the mean PPD and gain in CAL was found in group 2 compared to group 1 at 3- and 6-month intervals. Furthermore, a significantly greater bone fill was obtained in group 2 (1.70±0.54 mm) compared to group 1 (0.22±0.43 mm) after six months. Conclusion. ATV, as an adjunct to SRP, enhanced periodontal regeneration, as a noninvasive way to treat periodontal intraosseous defects.

The Usage of Low Level Laser Therapy Bio Stimulation and Bone Grafting in Accelerating the Healing of chronic intra-Osseous Defects: Vivo Study

The application of LLLT has become popular in the promotion of wound healing and reduction of pain following nonsurgical and surgical procedures. The aim of this article was to evaluate the efficiency of an SBX putty nano-bone graft with and without applying LLLT in accelerating the bone formation of the intra-bony defects through apical curettage without apicoectomy. In this study a total of 30 upper anterior maxillary teeth with periapical lesions. Patients were divided into two equal groups: Group I: apical curettage without apicoectomy and applying SBX putty nano-bone graft inside the intra-bony defects (n=15). Group II: apical curettage without apicoectomy, applying SBX putty nano-bone graft inside the intra-bony defects and then treating the defect with LLLT (n=15). CBCT was taken to determine the approximate size of periapical radiolucencies, All patients were followed up using CBCT for 1 year for evaluation of the size of the radiolucent area and bone density. Results showed that the differences in bone density measured on the panoramic as well as the cross-sectional views at all time intervals were non-significant (p<0.05) Conclusion: The Use of both SBX nano-bone graft and LLLT is considered an optimum choice for accelerating the healing of intra-bony defects.

Periodontal reconstructive surgery of deep intraosseous defects using an apical approach. Non-incised papillae surgical approach (NIPSA): A retrospective cohort study.

The objective of this study was to compare a minimally-invasive surgical technique (MIST) and a non-incised papilla surgical approach (NIPSA) in periodontal reconstructive surgery of deep intraosseous defects. Data on 30 patients with a deep intraosseous defect treated with MIST (n=15) or NIPSA (n=15) were analyzed retrospectively. All patients met the same inclusion criteria and were treated following the same protocol, except for the surgical management of soft tissue (MIST versus NIPSA). Clinical parameters at baseline and at 1-year post-surgery, early healing at 1week, and postoperative pain were assessed. NIPSA and MIST resulted in significant clinical attachment gain (CAG) (P<0.001) and probing depth reduction (PDr) (P<0.001) at 1-year post-surgery. However, NIPSA resulted in significantly lower recession of the tip of the interdental papilla compared with MIST (P<0.001). Smoking negatively influenced early healing in both techniques (P<0.05). NIPSA and MIST both resulted in significant improvements in clinical parameters. NIPSA showed significant soft tissue preservation. NIPSA may represent a promising papillae preservation technique in the treatment of intraosseous periodontal defects.

Regenerative Periodontal Treatment with the Single Flap Approach in Smokers and Nonsmokers.

The present study was performed to evaluate the impact of smoking status on 6-month clinical outcomes of a regenerative procedure based on enamel matrix derivative and deproteinized bovine bone mineral in the treatment of intraosseous defects accessed with buccal single flap approach. A total of 22 defects were selected in smoking (n = 11) and nonsmoking (n = 11) patients. Regenerative treatment resulted in similar clinical attachment level (CAL) gain in both smokers and nonsmokers. A trend toward lower 6-month CAL gain and probing depth reduction was observed in patients smoking 11 to 20 cigarettes per day compared to those smoking 1 to 10 cigarettes per day.

Simplified procedures to treat periodontal intraosseous defects in esthetic areas.

Successful treatment of deep intraosseous defects in esthetic areas implies clinical improvement of the lesion consistent with long-term tooth survival as well as the preservation (or improvement) of esthetics. It is hoped that such treatment end points may be reached through procedures (which we have termed as 'simplified') that, on the one hand can be easily and successfully applied by the majority of clinicians, and, on the other hand, are well tolerated by patients in terms of postsurgical pain and discomfort, adverse events and cost. In this review, we describe the technical aspects of the simplified procedures currently available (i.e. nonsurgical therapy and the single flap approach and its variants). Also, we analyze the effect of such procedures on clinical parameters and patient-centered outcomes. While nonsurgical therapy seems to be appropriate per se in lesions with a probing depth of < 7 mm and a limited intraosseous component, severe intraosseous defects can be successfully treated using simplified surgical procedures. Overall, data support the effectiveness of simplified surgical procedures and indicate that they result in minimal esthetic impairment (i.e. post-treatment recession) and a more tolerable postoperative course when compared with conventional surgical (double flap) approaches. In particular, the single flap approach was shown to be at least as effective as traditional papilla-preservation techniques when evaluated either as a stand-alone protocol or in combination with regenerative devices. Bioactive agents have shown the most appropriate regenerative technology when used in association with simplified surgical procedures.

Adjunctive Effect of Autologous Platelet-Rich Fibrin in the Treatment of Intrabony Defects in Localized Aggressive Periodontitis Patients: A Randomized Controlled Split-Mouth Clinical Trial.

The aim of this study was to investigate the clinical and radiologic efficacy of autologous platelet-rich fibrin (PRF) in the treatment of intrabony defects associated with localized aggressive periodontitis (LAP). A total of 30 sites, 2 sites per individual in 15 LAP patients, were treated with modified flap operation (MFO; Kirkland flap) alone or combined with autologous PRF. The study variables included plaque index, sulcus bleeding index, probing depth (PD), clinical attachment level (CAL), and gingival marginal level at baseline and 12 months postoperatively. The radiographic bone fill (RBF) on standardized radiographs was assessed after a year using image analysis software. The improvements in PD, CAL, and RBF in test sites compared to control sites were statistically significant (P < .05). Mean CAL gain and bone fill in the test sites were 4.0 ± 0.63 mm and 3.09 mm, respectively. Almost 80% of the PRF-treated sites showed ≥ 50% bone fill with minimal marginal tissue recession. Use of PRF significantly enhances the clinical and radiographic outcomes of open flap debridement in the treatment of periodontal intraosseous defects in patients affected by LAP.

Clinical and radiological assessment of the use of Bio-Gen Mix xenografts in jaw bone defects

Wstęp: Tradycyjne metody postępowania i wykonywania zabiegów z zakresu chirurgii stomatologicznej zazwyczaj prowadzą do bezpowrotnej utraty uzębienia i powstania typowego ubytku wysokości i szerokości kości grzbietu wyrostka zębodołowego. Celem niniejszego badania była ocena gojenia się ubytków kostnych po zastosowaniu materiału kościozastępczego Bio-Gen Mix.Materiały i metody: Grupę badaną stanowiło 42 pacjentów leczonych z powodu obecności torbieli zębopochodnych, zmian okołowierzchołkowych przy zębach wymagających przeprowadzenia zabiegu resekcji wierzchołka korzenia oraz pacjentów wymagających poekstrakcyjnych zabiegów augmentacyjnych, jak również zabiegów sterowanej regeneracji kości przed planowanymi zabiegami implantacji z użyciem ksenogennego materiału kościozastępczego Bio-Gen Mix.Wyniki: Wyniki oceny radiologicznej i klinicznej podsumowano za pomocą 3-stopniowej skali oceniającej proces gojenia ubytków śródkostnych, zaproponowanej przez Gutmanna i Harrison, a zmodyfikowanej przez Taschieri i wsp.Wnioski: W przypadku niewielkich deficytów tkanki kostnej wystarczające jest wykorzystanie substytutów kości, chociażby preparatów pochodzenia wołowego, takich jak Bio-Gen Mix. Uzyskuje się w ten sposób dobry rezultat, dość szybką przebudowę tkanki kostnej, której jakość w dużej mierze wystarcza do prowadzenia dalszego leczenia implantoprotetycznego.

Kliniczna i radiologiczna ocena zastosowania materiału Bio-Gen Mix w ubytkach kości szczęk

Wstęp: Tradycyjne metody postępowania i wykonywania zabiegów z zakresu chirurgii stomatologicznej zazwyczaj prowadzą do bezpowrotnej utraty uzębienia i powstania typowego ubytku wysokości i szerokości kości grzbietu wyrostka zębodołowego. Celem niniejszego badania była ocena gojenia się ubytków kostnych po zastosowaniu materiału kościozastępczego Bio-Gen Mix.Materiały i metody: Grupę badaną stanowiło 42 pacjentów leczonych z powodu obecności torbieli zębopochodnych, zmian okołowierzchołkowych przy zębach wymagających przeprowadzenia zabiegu resekcji wierzchołka korzenia oraz pacjentów wymagających poekstrakcyjnych zabiegów augmentacyjnych, jak również zabiegów sterowanej regeneracji kości przed planowanymi zabiegami implantacji z użyciem ksenogennego materiału kościozastępczego Bio-Gen Mix.Wyniki: Wyniki oceny radiologicznej i klinicznej podsumowano za pomocą 3-stopniowej skali oceniającej proces gojenia ubytków śródkostnych, zaproponowanej przez Gutmanna i Harrison, a zmodyfikowanej przez Taschieri i wsp.Wnioski: W przypadku niewielkich deficytów tkanki kostnej wystarczające jest wykorzystanie substytutów kości, chociażby preparatów pochodzenia wołowego, takich jak Bio-Gen Mix. Uzyskuje się w ten sposób dobry rezultat, dość szybką przebudowę tkanki kostnej, której jakość w dużej mierze wystarcza do prowadzenia dalszego leczenia implantoprotetycznego.

Alveolar Ridge Augmentation Using Dystopic Autogenous Tooth: 2-Year Results of an Open Prospective Study.

To evaluate the use of chemically unchanged tooth material in lateral alveolar ridge augmentation or for the filling of jaw defects. A total of 20 patients underwent either lateral augmentation of the alveolar process (11 patients) or filling of jaw defects (6 patients) with autogenous unaltered tooth material in a longitudinal 2-year study. In three patients, the jaw defect was so marked that a bone block graft had to be used for augmentation in addition to particulate dental material. In four patients, an autogenous tooth block was exclusively used; in seven, crushed tooth material was exclusively used; and in the remaining six, dystopic teeth that had been extracted were removed, crushed, and reinserted into the defect in particulate form. Fully impacted teeth served as autogenous donor teeth. After a healing time of 3 to 6 months, 28 implants could be placed (10 immediate implants, 18 delayed implants). At 6, 12, and 24 months postrestoration, peri-implant bone loss as assessed by x-ray was 0 mm, 0.4 mm, and 0.6 mm, respectively. Peri-implant probing depth was 1 mm after 1 year and 2 mm after 2 years. Bleeding on probing was not seen in any of the implants after 2 years. Autogenous tooth material appears to be suitable for the restoration of lateral and intraosseous defects of the alveolar ridge with both complete blocks and in particulate form. However, additional long-term studies with higher case numbers will be required for substantiating these results.

Effect of 1% sodium alendronate in the non-surgical treatment of periodontal intraosseous defects: a 6-month clinical trial.

Background and objectives Few studies have evaluated the effect of the topical application of sodium alendronate (ALN) on the treatment of intrabuccal bone defects, especially those caused by periodontitis. This 6-month randomized placebo controlled clinical trial aimed at evaluating the effect of non-surgical periodontal treatment associated with the use of 1% ALN, through clinical evaluations and cone-beam computed tomography (CBCT).Material and Methods Twenty individuals with chronic periodontitis underwent periodontal examination at the baseline as well as 3 and 6 months after periodontal treatment, registering clinical attachment level (CAL), periodontal probing depth (PPD), and bleeding on probing (BOP) as the clinical outcomes. After manual scaling and root planing, 40 bilateral sites with interproximal vertical bone defects were randomly treated with either 1% ALN gel or a placebo. Bone defects were evaluated through CBCT at the baseline and 6 months post-treatment. The clinical and CBCT parameters were compared using the Wilcoxon and Friedman tests (p<0.05).Results Although ALN produced a greater CAL gain when compared to the placebo at 6 months post-treatment (p=0.021), both treatments produced similar effects on the PPD, BOP, and bone height. Significant differences in bone fill were observed only in patients of the ALN group (4.5 to 3.8 mm; p=0.003) at 6 months post-treatment.Conclusions Topical application of 1% ALN might be a beneficial adjuvant to non-surgical periodontal therapy.

Weichgewebsaugmentation in der regenerativen Parodontalchirurgie

Trombelli L et al. Effect of a Connective Tissue Graft in Combination with Single Flap Approach in the Regenerative Treatment of Intraosseous Defects. J Periodontol 2017; DOI: 10.1902/jop.2016.160471

Effect of a Connective Tissue Graft in Combination With a Single Flap Approach in the Regenerative Treatment of Intraosseous Defects [Formula: see text

In the attempt to limit the post-surgery increase in buccal gingival recession (bREC), effect of a connective tissue graft (CTG) when combined with a buccal single flap approach (SFA) in the regenerative treatment of intraosseous defects is evaluated. Data related to 30 patients with an intraosseous defect treated with a buccal SFA with (SFA+CTG group; n = 15) or without (SFA group; n = 15) placement of a CTG and regenerative treatment were retrospectively derived at three clinical centers. bREC and probing parameters were assessed at presurgery and 6 months post-surgery. In addition to a significant attachment gain and probing depth reduction, adjunctive use of a CTG to a buccal SFA in the regenerative treatment of periodontal intraosseous defects associated with a buccal bone dehiscence resulted in a limited post-surgery bREC, a lower prevalence of defects with a clinically detectable apical displacement of the gingival margin, and an increase in gingival width and thickness. Adjunctive use of a CTG in the regenerative treatment of intraosseous defects associated with buccal bone dehiscence accessed by buccal SFA may support the stability of the gingival profile.

Aggressive central giant cell granuloma of the maxilla in a patient with neurofibromatosis type 1 — case report and review of literature

Background: Central giant cell granuloma (CGCG) is a benign osseous lesion that predominantly involves jaws with variably aggressive behaviour. It characterised by accumulation of multinucleated giant cells in a background of cellular vascular fibrous tissue and spindle-shaped stromal cells. Neurofibromatosis type 1 (NF-1) is an autosomal dominant trait associated with multiple neurofibromas, cafe-au-lait pigmented skin lesions, optic nerve glioma, Lisch nodules and distinct osseous dysplasia. In 1971 Kerl reported multiple giant cell granulomas in a patient with Recklinghausen's neurofibromatosis, few literatures showed this rare coincidence. Objectives: We report a case of NF-1 with aggressive maxillary CGCG and reviewed on the proposed coincidental association or true genetic linkage. Methods: A complete search on the web was done and all the related articles studied and concluded. Findings and Conclusion: In NF-1 patients genetic drawbacks such as NF-1 haploinsufficiency or underling mechanisms that cause decreased neurofibromin function, may result in decreased bone ability to respond to functional demands, thus the skeleton is more prone to develop intraosseous defects.

Comparison of Different Periodontal Healing of Critical Size Noncontained and Contained Intrabony Defects in Beagles.

Background:Regenerative techniques help promote the formation of new attachment and bone filling in periodontal defects. However, the dimensions of intraosseous defects are a key determinant of periodontal regeneration outcomes. In this study, we evaluated the efficacy of use of anorganic bovine bone (ABB) graft in combination with collagen membrane (CM), to facilitate healing of noncontained (1-wall) and contained (3-wall) critical size periodontal defects.Methods:The study began on March 2013, and was completed on May 2014. One-wall (7 mm × 4 mm) and 3-wall (5 mm × 4 mm) intrabony periodontal defects were surgically created bilaterally in the mandibular third premolars and first molars in eight beagles. The defects were treated with ABB in combination with CM (ABB + CM group) or open flap debridement (OFD group). The animals were euthanized at 8-week postsurgery for histological analysis. Two independent Student's t-tests (1-wall [ABB + CM] vs. 1-wall [OFD] and 3-wall [ABB + CM] vs. 3-wall [OFD]) were used to assess between-group differences.Results:The mean new bone height in both 1- and 3-wall intrabony defects in the ABB + CM group was significantly greater than that in the OFD group (1-wall: 4.99 ± 0.70 mm vs. 3.01 ± 0.37 mm, P < 0.05; 3-wall: 3.11 ± 0.59 mm vs. 2.08 ± 0.24 mm, P < 0.05). The mean new cementum in 1-wall intrabony defects in the ABB + CM group was significantly greater than that in their counterparts in the OFD group (5.08 ± 0.68 mm vs. 1.16 ± 0.38 mm; P < 0.05). Likewise, only the 1-wall intrabony defect model showed a significant difference with respect to junctional epithelium between ABB + CM and OFD groups (0.67 ± 0.23 mm vs. 1.12 ± 0.28 mm, P < 0.05).Conclusions:One-wall intrabony defects treated with ABB and CM did not show less periodontal regeneration than that in 3-wall intrabony defect. The noncontained 1-wall intrabony defect might be a more discriminative defect model for further research into periodontal regeneration.

Use of Platelet Rich Plasma in the Management of Periodontal Intra-Osseous Defects: A Clinical Study.

Background:Periodontal disease is characterized by the presence of gingival inflammation, periodontal pocket formation, loss of connective tissue attachment, and alveolar bone around the affected tooth. Alveolar bone support and attachment apparatus regeneration has been achieved through various processes and have given elusive results. An expedient and cost-effective approach to obtain autologous platelet-derived growth factor (PDGF) and transforming growth factor (TGF)-β is the use of platelet-rich plasma (PRP). PRP is obtained by sequestrating and concentrating platelets by gradient density centrifugation.Aims:The current study was aimed at evaluating the regenerative potential of platelet-rich plasma in comparison with open flap debridement.Settings and Designs:This study was a randomized controlled clinical trial conducted in the Department of Periodontics and Oral Implantology, KIDS, Bhubaneswar, Odisha.Materials and Methods:Twenty periodontal infrabony defects in 10 patients; 6 males and 4 females of age between 25–45 years were included in this study and were followed up for a period of 6 months.Statistical Analysis:Both the groups showed a mean plaque index of 2.10 and 2.50 at baseline, 1.75 and 2.05 at 3 months, and 1.28 and 1.53 at the end of 6 months. The mean reduction of 0.35 and 0.45 at three months and 0.82 and 0.97 at six months was achieved, which was statistically significant. (P < 0.001). When comparison was done between the two groups it was not found to be statistically significant (P < 0.05). In each of the group there was definitive reduction in plaque score over a period of time.Results and Conclusion:There was no statistically significant difference in the treatment outcome between open flap debridement and PRP alone. Platelet-rich plasma application holds promise and needs further exploration.

QUANTIFICATION OF INTRA OSSEOUS BONE DEFECTS BY CONVENTIONAL VERSUS 3DIMENSIONAL IMAGING TECHNIQUES - AN IN VIVO STUDY.

Background:- The aim of this prospective cross sectional study was to determine the accuracy of CBCT in quantifying intra-osseous periodontal bone defects. Methods:- 5 patients with intra-bony defects were selected and 10 defects were assessed. A total of 60 measurements were performed.Periapical radiographs and Cone beam CT scan images were obtained. Height and depth of each defects was measured using appropriate software. Direct measurements were done during surgical interventions using a periodontal probe and were considered the standard reference. Measurements made by all three modalities were compared to each other. Results:- Linear measurements for all defects revealed no statistical differences between CBCT and direct intra-surgical measurements with respect to the height as well as the depth of the defect. There was a significant difference when comparing peri-apical radiographs to the other two methods. IOPA measurements were only 74.3% accurate as compared to the standard intra-surgical whereas the CBCT measurements were 86.5%. Conclusion:- All three modalities proved to be useful for identifying interproximal periodontal defect but CBCT took the lead with better accuracy in reproducing the clinical measurement of intra-bony periodontal bone defects and better visualization of the extent of the defect.

Clinical Evaluation of Insulin like Growth Factor-I and Vascular Endothelial Growth Factor with Alloplastic Bone Graft Material in the Management of Human Two Wall Intra-Osseous Defects.

In recent years, emphasis on the use of growth factors for periodontal healing is gaining great momentum. Several growth factors showed promising results in periodontal regeneration. This study was designed to compare the clinical outcomes of 0.8μg recombinant human Vascular Endothelial Growth Factor (rh-VEGF) and 10μg recombinant human Insulin Like Growth Factor-I (rh-IGF-I) with β-Tricalcium Phosphate (β-TCP) and Polylactide-Polyglycolide Acid (PLGA) membrane in two wall intra-osseous defects. A total of 29 intra-osseous defects in 27 subjects were randomly divided into 3 test and 1 control group. Test group I (n=8) received rh-VEGF+ rh-IGF-I, Test group II (n=7) rh-VEGF, Test group III (n=7) rh-IGF-I and control group (n=7) with no growth factor, β-TCP and PLGA membrane was used in all the groups. Baseline soft tissue parameters including Probing Pocket Depth (PPD), Clinical Attachment Level (CAL), and Gingival Recession (GR) at selected sites were recorded at baseline and at 6 months. Intrasurgically, intra-osseous component was calculated as a) Cemento-Enamel Junction to Bone Crest (CEJ to BC), b) Bone Crest to Base of the Defect (BC to BD) at baseline and at re-entry. The mean changes at baseline and after 6 months within each group were compared using Wilcoxon Signed Rank Test. The mean changes for each parameter between groups were compared using Mann-Whitney U test. After 6 months, maximum mean PPD reduction occurred in test group I followed by test group II, III and control group. Similar trend was observed in CAL gain. Non-significant GR was present in test group I and control group whereas in test group II and III GR was absent. The use of rh-VEGF+ rhIGF-I exhibited 95.8% osseous fill as compared to 54.8% in test group II, 52.7% in test group III and 41.1 % in the control group. Within the limitations of this study, it can be concluded that, rh-IGF-I+rh-VEGF treated sites resulted in greater improvement in PPD reduction, CAL gain as well as in osseous fill after 6 months when compared with rh-VEGF, rh-IGF-I and control sites.

Comparative clinical and radiographic evaluation of mineralized cancellous bone allograft (puros®) and autogenous bone in the treatment of human periodontal intraosseous defects: 6-months follow-up study.

Aims:Several materials have been introduced as bone grafts, i.e., autografts, allograft, xenografts, and alloplastic grafts, and studies have shown them to produce greater clinical bone defect fill than open flap debridement alone. The aim of this clinical and radiological 6-month study was to compare and evaluate the clinical outcome of deep intraosseous defects following reconstructive surgery with the use of mineralized cancellous bone allograft (Puros®) or autogenous bone.Materials and Methods:Ten patients with 12 sites exhibiting signs of moderate generalized chronic periodontitis were enrolled in the study. The investigations were confined to two and three-walled intra bony defects with a preoperative probing depth of ≥5 mm. Six of these defects were treated with Puros® (group A) the remaining six were treated with autogenous bone graft (group B). Allocation to the two groups was randomized. The clinical parameters, plaque index (PI), gingival index (GI), probing pocket depth (PPD), clinical attachment level (CAL), and bone fill, were recorded at different time intervals at the baseline, 1 month, 3 months, and 6 months. Intraoral radiographs were taken using standardized paralleling cone technique at baseline, 1, 3, and 6 months. Statistical analysis was done by using the one-way analysis of variance (ANOVA) followed by Tukey highly significant difference.Results:Both groups resulted in decrease in probing depth (group A, 3.0 mm; group B, 2.83 mm) and gain in clinical attachment level (group A, 3.33 mm; group B, 3.0 mm) over a period of 6 months, which was statistically insignificant.Conclusion:Within the limitations of the present study, it can be concluded that both mineralized cancellous bone allograft (Puros®) or autogenous bone result in significant clinical improvements.