To evaluate the preoperative clinical and biometric determinants associated with the actual lens position after cataract surgery. Department of Ophthalmology, University Hospital of Montpellier, France. Prospective longitudinal cohort study. The data collected included clinical factors (age, sex, history of vitrectomy) and biometry factors (axial length [AL], anterior chamber depth [ACD], lens thickness, white-to-white [WTW] distance) that might affect actual lens position. Each patient had optical low-coherence reflectometry biometry (Lenstar) preoperatively and 1month postoperatively. The actual lens position was measured as the postoperative position of the center of the intraocular lens (IOL). Patients were stratified into 3 groups by type of IOL: Acrysof SN60WF or SN6AT (Group 1), Tecnis ZCB00 or ZCT (Group 2), and Asphina 409MV (Group 3). The study comprised 168 eyes (mean age 73.3years±9.8 [SD]). The mean actual lens position was 4.88±0.29mm, 5.01±0.29mm, and 5.05±0.32mm in Group 1 (n=67 eyes), Group 2 (n=52 eyes), and Group 3 (n=49 eyes), respectively. In the overall population, AL, ACD, anterior segment depth, and WTW distance were correlated with actual lens position (r=0.48, P<.0001; r=0.64, P<.001; r=0.58, P<.0001; r=0.39, P<.001, respectively). The AL, ACD, anterior segment depth, and WTW distance correlated with actual lens position after cataract surgery. The integration of these data in IOL formulas could help improve refractive outcomes after the surgery.