Intramolecular Hydrolysis Research Articles

Spin-labeled Sulfonyl Fluorides as Active Site Probes of Protease Structure: II. SPIN LABEL SYNTHESES AND ENZYME INHIBITION

Abstract The syntheses and enzyme inhibition results for the derivatives discussed in the preceding paper are given. Spin-labeled sulfonyl fluorides containing six-membered piperidinyl nitroxide rings were particularly susceptible to intramolecular hydrolysis. In some cases, however, this hydrolysis was retarded significantly by the enzyme environment.