Intraerythrocyte Sodium Research Articles

Blood pressure and intra-erythrocyte sodium during normal and high salt intake in middle-aged men: relationship to family history of hypertension, and neurogenic and hormonal variables.

During 4 weeks 37 normotensive 50-year-old men identified by screening in a random population sample were given 12 g of NaCl daily, in addition to their usual dietary sodium intake. Blood pressure, heart rate, weight, urinary excretion of sodium, potassium and catecholamines, plasma aldosterone and noradrenaline and intra-erythrocyte sodium content were determined on normal and increased salt intake. The subjects were divided into those with a positive family history of hypertension (n = 11) and those without such a history (n = 26). Systolic blood pressure and weight increased significantly irrespective of a positive family history of hypertension. On normal salt intake intra-erythrocyte sodium content was significantly higher in those with a positive family history of hypertension. During high salt intake intra-erythrocyte sodium content decreased significantly in that group and the difference between the hereditary subgroups was no longer significant. In the whole group urinary excretion of noradrenaline, adrenaline and dopamine increased whereas plasma aldosterone decreased during the increased salt intake. Thus, in contrast to some earlier studies performed in young subjects, our results indicate that moderately increased sodium intake acts as a pressor agent in normotensive middle-aged men whether there was a positive family history of hypertension or not. We confirm that men with positive family history of hypertension have an increased intra-erythrocyte sodium content, and that an increase in salt intake seems to increase overall sympathetic activity.

34 Relationship Between Intra-erythrocyte Sodium Content and Cation Transport in Hypertension, Hyperthyroidism, Pregnancy and Hypokalaemia

Gless, K H; Sütterlin, U; Schaz, K; Gross, P; Schütz, V; Hunstein, W Author Information

Relationship between the intra-erythrocyte sodium composition and the membrane lipoprotein composition among different mammal species

1. 1. Internal sodium and lipoprotein composition of RBCs of nine mammalian species are measured. 2. 2. A significant correlation can be demonstrated between the erythrocyte mean sodium value of studied species and the membrane protein/lipid ratio ( r = 0.80, α < 0.01). 3. 3. Erythrocyte internal sodium can be correlated with membrane-free cholesterol but not with the phospholipid fractions.

Blood pressure, intraerythrocyte content, and transmembrane fluxes of sodium during normal and high salt intake in subjects with and without a family history of hypertension: evidence against a sodium transport inhibitor.

Seventeen young normotensive men with a family history of hypertension in two generations (H) and 15 age-matched control subjects (C) were studied with respect to blood pressure (BP), intraerythrocyte sodium content (IeNa), sodium influx, and rate of sodium efflux. The investigations were done during normal salt intake and after 4 weeks of ordinary intake plus 12 g NaCl daily. BP did not increase significantly in either of the two groups during increased salt intake. During normal salt intake H had a significantly (p less than 0.01) higher IeNa (9.5 +/- 1.5 mmol/L) compared with C (8.2 +/- 1.4 mmol/L). During high salt intake IeNa in H decreased significantly to 8.1 +/- 1.2 mmol/L, the difference from C (7.6 +/- 1.2 mmol/L) not being significant. While the Na influx was similar in the two groups, the rate constant for Na efflux was significantly lower during normal salt intake in H (0.23 +/- 0.08 vs 0.29 +/- 0.1 h-1, p less than 0.05). Salt intake increased the efflux rate constant significantly in H (0.28 +/- 0.08 h-1, p less than 0.05), while it did not change significantly in C (0.32 +/- 0.08 h-1) compared with the value for normal salt intake. Our results suggest that young men with a hereditary predisposition to hypertension have a higher IeNa secondary to a lower rate of Na efflux, while a normal Na influx indicates normal cell permeability to Na. The findings in H during high Na intake--a decreased IeNa and an increased efflux rate of Na--do not favor the existence of a sodium transport inhibitor, in subjects predisposed to hypertension, increasing during high salt intake and volume expansion and acting through inhibition of the Na efflux.

Abnormal red cell sodium content and efflux in Japanese patients with essential hypertension.

Net sodium (Na) efflux and potassium (K) influx were determined in Na-loaded/K-depleted erythrocytes derived from 37 patients with essential hypertension and 25 age-matched normotensive subjects with no family history of hypertension, together with the measurement of basal red cell sodium and potassium contents. Intraerythrocyte sodium content was significantly higher in the essential hypertensives than in the normotensives (10.9 +/- 1.4 vs 10.0 +/- 1.2 mmol/L X cells, mean +/- SD, p less than 0.02), but potassium content was nearly equal between the two groups. Net Na efflux in the hypertensives was significantly reduced compared with that in the normotensives (4.57 +/- 0.70 vs 5.18 +/- 1.02 mmol/L X cells X hr, p less than 0.01), but both net K influx and net Na/K flux ratio were not significantly different between the two groups. Net Na efflux and K influx showed a significant inverse correlation with red cell sodium content (r = -0.64 and r = -0.56, respectively, p less than 0.001). These results suggest that the reduced net Na efflux with the increase of red cell sodium content may be related to the pathogenesis of essential hypertension. However, it is impossible to determine the genetic marker of essential hypertension by using the net Na/K flux ratio of Japanese subjects, although Garay et al. have reported that this index was abnormally low in the case of Europeans.

Erythrocyte Membrane and Renal Function in Relation to Hypertension in Rats of the Milan Hypertensive Strain

1. The aim of this study was to compare certain renal and erythrocyte functions in the Milan hypertensive strain (MHS) of rats and in normotensive control animals (MNS). 2. Under experimental conditions close to those in conscious unmanipulated animals, the glomerular filtration rate (GFR) per g kidney weight was higher and the kidney weight/body weight ratio lower in MHS than in MNS rats (P &amp;lt; 0.001). This suggests that there is increased transtubular ion transport in MHS animals. 3. The MHS rats had a higher rate constant for outward Na+-K+ cotransport than the MNS animals (P &amp;lt; 0.02), their intra-erythrocyte sodium concentrations were lower (P &amp;lt; 0.05) and their erythrocyte volume was smaller (P &amp;lt; 0.001). Therefore, accelerated cell membrane sodium outward cotransport seems to reset the volume and the sodium content of the erythrocytes of MHS rats at a lower level. 4. We speculate that increased transport of ions across the cell membrane may be an abnormality common to erythrocytes and renal tubular cells in MHS rats.

Intra-erythrocyte sodium and (Na+,K+-activated)-ATPase concentration and urinary aldosterone excretion in spontaneously hypertensive rats.

1. Intra-erythrocyte sodium, potassium, ATP and (Na+,K+-activated)-ATPase concentrations and urinary aldosterone excretion were compared in 3-month-old spontaneously hypertensive rats (n = 11) and normotensive Wistar-Kyoto control rats (n = 11). 2. Spontaneously hypertensive rats exhibited significantly higher intra-erythrocyte sodium concentration (5.5 +/- 1.3 vs 4.0 +/- 1.1 mmol/l of erythrocytes, P less than 0.01). No significant difference was found in intra-erythrocyte potassium. ATP or (Na+,K+-activated)-ATPase concentration. 3. Mean urinary aldosterone excretion was significantly lower in spontaneously hypertensive rats (66.3 +/- 6.5 pmol/24 h) than in Wistar-Kyoto rats (90.5 +/- 10.6 pmol/24 h, P less than 0.01). No significant relationship between urinary aldosterone and intra-erythrocyte sodium concentration was found in spontaneously hypertensive or Wistar-Kyoto rats or in the pooled group. 4. These results are thus consistent with previous findings of an increased intracellular sodium concentration in spontaneously hypertensive rats, but do not support the hypothesis that aldosterone is a dominant regulator of intracellular sodium concentration.

Plasma and Erythrocyte Cations in Affective Illness

Plasma and erythrocyte cations (sodium and magnesium) were studied in groups of patients with an affective disorder and in normal subjects. Baseline determinations were obtained before initiation of treatment for mania or depression. In a subgroup of patients, sequential measurements of cations were made during treatment with lithium carbonate. No differences were found in intraerythrocyte sodium or magnesium among any of the patient groups and controls. Patients with a primary affective disorder had significantly higher plasma sodium than control subjects. Neither baseline cation concentrations nor changes in cation concentration during treatment with lithium correlated with treatment response. Gender was shown to be a significant variable affecting intraerythrocyte cation concentrations.

Sodium efflux in rabbit erythrocytes.

ARTICLESSodium efflux in rabbit erythrocytesO Rettori, V Rettori, JV Maloney, and MF VillamilO Rettori, V Rettori, JV Maloney, and MF VillamilPublished Online:01 Aug 1969https://doi.org/10.1152/ajplegacy.1969.217.2.605MoreSectionsPDF (970 KB)Download PDF ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInEmailWeChat Previous Back to Top Next Download PDF FiguresReferencesRelatedInformationCited ByRelationship between the intra-erythrocyte sodium composition and the membrane lipoprotein composition among different mammal speciesComparative Biochemistry and Physiology Part A: Physiology, Vol. 77, No. 2Cation movements across mouse red blood cellsBiochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 601Studies on lithium transport across the red cell membraneThe Journal of Membrane Biology, Vol. 51, No. 3-4Potassium transport and lipid composition in mammalinan red blood cell membranesBiochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 464, No. 1Atpases in rabbit erythrocytes: Stimulation by HCO3− and by Na+-plus-K+Life Sciences, Vol. 16, No. 6Effects of extracellular cations and ouabain on catecholamine-stimulated sodium and potassium fluxes in turkey erythrocytes.Journal of Biological Chemistry, Vol. 250, No. 4Comparative aspects of phosphate transfer across mammalian erythrocyte membranesThe Journal of Membrane Biology, Vol. 13, No. 1Cation transport and membrane morphologyThe Journal of Membrane Biology, Vol. 12, No. 1A two-site model for sodium transport in rabbit erythrocytesBiochemical and Biophysical Research Communications, Vol. 46, No. 3Erythrocyte membranes-effect of increased cholesterol content on permeabilityBiochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 249, No. 2 More from this issue > Volume 217Issue 2August 1969Pages 605-608 Copyright & PermissionsCopyright © 1969 by American Physiological Societyhttps://doi.org/10.1152/ajplegacy.1969.217.2.605PubMed5799392History Published online 1 August 1969 Published in print 1 August 1969 Metrics