To assess the differences in surgical outcomes between bidirectional barbed suture and conventional suture for myoma bed closure during laparoscopic myomectomy. Study design: Ninety-two patients with one to three symptomatic myomas were randomized in a 1:1 ratio to the barbed suture group or the conventional suture group. Myoma bed was sutured either with barbed suture (STRATAFIX™, Ethicon Inc, USA) or continuous conventional suture with intracorporeal knot tying (Vicryl; Ethicon, USA). The surgeon assessed the degree of suturing difficulty using a visual analog scale ranging from 1 (least difficult suturing) to 10 (most difficult suturing). Results: Suturing with barbed suture was easier than suturing with conventional suture (3.04±1.47 Vs. 4.75± 1.35, P < 0.001). Suturing time of the myoma bed was significantly shorter in the barbed suture group (14.98±4.81 Vs 22.09±6.9 min; P < 0.001). Operative time was comparable between both groups (69.67±17.63 Vs 74.15±19.79 min; P = 0.255). The mean intraoperative blood loss was significantly higher in conventional suture group (337.24±234.15 ml Vs 211.3±107.62; P= 0.002). Conclusion: The barbed suture facilitates suturing of the myoma bed during laparoscopic myomectomy. Compared with conventional suture, barbed suture reduces the time needed to suture the myoma bed and the intraoperative blood loss.