Intracanal Irrigant Research Articles

A Systematic Review of the Comparative Efficacy of Lactobacillus Probiotics and Sodium Hypochlorite as Intracanal Irrigants Against Enterococcus faecalis.

Irrigation plays a pivotal role in the success of root canal treatments. The development of innovative, less hazardous irrigating solutions is necessary because of the inherent limitations of the gold standard, sodium hypochlorite. Since probiotics have proven to be effective in treating common oral diseases such as periodontitis and dental caries, they have gained attention in the field of endodontics as well. The present systematic review aims to assess the efficacy of Lactobacillus probiotics against Enterococcus faecalis compared to that of sodium hypochlorite. A thorough search of five databases, PubMed, Scopus, EBSCOhost, ScienceDirect, and BVS (Biblioteca Virtual en Salud, or Virtual Health Library), from January 2000 to January 2024 yielded 135 articles after a preliminary search. Three research publications that satisfied the strict inclusion and exclusion criteria were charted after the removal of duplicates and careful examination of the full-text articles. The Quality Assessment Tool for In Vitro Studies (QUIN Tool) was used to assess the quality of the study, aiming to identify any risk of bias. All three publications hada low risk of bias and demonstrated that Lactobacillus species were effective against E. faecalis and proved to be a safer alternative to sodium hypochlorite as an intracanal irrigant. However, more clinical trials are required to determine the best probiotic combinations, appropriate probiotic carriers, andideal dosage and frequency of administration before using probiotics as intracanal irrigants in clinical trials.

Effectiveness of Two Intracanal Irrigation Solutions Delivered Through Cryotherapy on Post-Endodontic Pain Relieve. A Randomized Clinical Trial

Effectiveness of Two Intracanal Irrigation Solutions Delivered Through Cryotherapy on Post-Endodontic Pain Relieve. A Randomized Clinical Trial

Effectiveness of white tea-mediated silver nanoparticles as an intracanal irrigant against Enterococcus faecalis: An in vitro study.

The probability of a positive outcome of root canal therapy is substantially higher if the infection is eradicated successfully before the obturation of the root canal system. Irrigation is an essential aspect of root canal debridement, as it enables more thorough cleaning than is possible with root canal instrumentation alone. To overcome the side effects of chemical irrigants, there has been a search for herbal medicines as substitutes. The aim of the present study was to explore the antimicrobial efficacy of white tea-mediated silver nanoparticles (AgNPs) formulated as an intracanal irrigant against Enterococcus faecalis, and to compare it with the efficacy of chlorhexidine and sodium hypochlorite irrigants. The experimental groups were as follows: group I - white tea-mediated AgNPs; group II - 2% chlorhexidine; and group III - 2.5% sodium hypochlorite. The characterization of AgNPs was performed using ultraviolet-visible (UV-Vis) spectroscopy and transmission electron microscopy (TEM) analysis. Enterococcus faecalis was inoculated onto Mueller-Hinton agar plates. The disks impregnated with irrigants were placed on the inoculated plates and incubated aerobically at 37°C for 24 h. Then, the growth inhibition zones were measured. Statistical analysis was performed using the one-way analysis of variance (ANOVA) and the post hoc tests. A concentration of 50 μL of white tea-mediated AgNPs exhibited the greatest zone of inhibition (32 ±2 mm), followed by 2% chlorhexidine (25 ±1 mm) and 2.5% sodium hypochlorite (23 ±3 mm). White tea-mediated AgNPs showed promising results in the elimination of E. faecalis, being superior to chlorhexidine and sodium hypochlorite irrigants.

Potential Use of Propolis as Endodontic Irrigant

Objective of this work was to review the use of propolis as an endodontic irrigant. For this, keywords were searched in the PubMed database, from April to October 2022. There are many chemical compounds in propolis from different geographic regions; Flavonoids are one of the most important agents that have anti-inflammatory, anti-viral, anti-allergic, anti-cancer, anti-bacterial and antioxidant effects. According to the mentioned properties, propolis can be used as a canal irrigation solution, as well as intracanal medicine in endodontic treatments. The results described in the literature are practically unanimous in showing the antibacterial and antifungal effects of different propolis extracts at different concentrations. In addition to flavonoids, other components of propolis such as resin, pollen, vitamins and phenols, indicate that propolis can be used not only as an intracanal irrigation solution, but for various purposes, with a promising role in endodontics as well as dentistry.

Management of Endo-Perio Lesion using Collagen Membrane: A Case Report

This case report describes the management of endo-perio lesion. A 40 year old patient reported with pain and discolored tooth with history of road traffic accident a year ago. Intraoral examination of tooth 11 revealed the presence of restorative material and a sinus tract opening with probing depth of 6mm. Following full mouth scaling, access opening and biomechanical preparation was done during which intra-canal irrigant was seen oozing through the sulcus of tooth 11. Multiple RVGs were taken as there was difficulty in negotiating the canal which suggested the presence of communication. Surgical intervention was carried out were root perforation was noted. Canal was negotiated and perforation was repaired using biodentine. Collagen membrane was placed as a barrier. Patient was recalled after 2 weeks, 1 month and at 3months. At the end of three months there was complete reduction in probing depth with satisfactory soft tissue healing. Proper diagnosis of the endo-perio lesion is the key to success. The multidisciplinary approach with accurate diagnosis contributed to the favorable therapeutic outcome.

Comparative Evaluation of Effectiveness of Nisin, Amoxicillin/Clavulanic Acid (Augmentin) and Chlorhexidine on E. Faecalis as an Intracanal Irrigant: An In-vitro Study.

The present study evaluated the antimicrobial efficacy of chlorhexidine gluconate (CHX), Nisin, and Amoxicillin/Clavulanic Acid (Augmentin) as an intracanal irrigant against Enterococcus faecalis (EF). Minimum Inhibitory Concentration (MIC) for EF against Nisin and Augmentin was determined by microbroth dilution technique. Time kill cycle (TKC) analysis was done for 0 MIC, ½ MIC, 1 MIC, and 2 MIC at 0 hour, 15 minutes, 30 minutes, and 45 minutes. At the end of each time period, dilutions were pipetted and swabs of agar plates were done. Incubation of agar plates was done at 37C for 24 hours. Colonies formed were counted. The time kill curve analysis of EF for CHX, Nisin, and Augmentin at different concentrations and time periods showed a gradual decline in mean bacterial count between 0 and 45 minutes for CHX; this decline increases with increase in concentrations and time. Whereas in group Nisin, not much decline in bacterial count is noted for ½ MIC concentrations but a signification reduction of P < 0.001 after exposure to Nisin at 1 MIC ant 2 MIC concentrations. Group Augmentin showed not much reduction in bacterial count with increase in concentration and time. From this study, Nisin is found to be a promising agent in eliminating EF in comparison to other irrigants tested. However, the systemic effect of this irrigant, its biocompatibility, allergic potential, and bacterial resistance needs further investigation.

Antimicrobial efficacy of superoxide water as an intracanal irrigant in primary anterior teeth against facultative anaerobes: A randomized clinical trial

ABSTRACT Background: There is always a call for a search for an irrigating solution that can mask the disadvantages and simultaneously have antimicrobial action when used in root canals (RCs) at par with that of the most commonly used irrigants. Aim: The aim of this study was to evaluate the efficacy of superoxide water (SOW) to 1% sodium hypochlorite (NaOCl) and 2% chlorhexidine gluconate (CHX-G) when used as RC irrigants in primary anterior teeth against facultative anaerobes. Materials and Methods: A triple-blinded randomized clinical trial was carried out among children between the age groups of 3 and 7 years. Sixty teeth were randomly grouped into three groups: Group I: 1% NaOCl irrigation, Group II: 2% CHX-G irrigation, and Group III: SOW. Pre- and postirrigation samples using the paper points were sent to the microbial laboratory for the culture. Chi-square analysis and analysis of variance were carried out to find the significance of study parameters on the categorical scale and between the groups, respectively. Results: All the three irrigating solutions showed a statistically significant reduction in the microbial count of facultative anaerobes when pre- and postirrigating samples were compared. Conclusion: All the three solutions had almost similar antimicrobial efficacy. SOW was found to have similar effects on facultative anaerobes as that of NaOCl. Hence, SOW can be used as an alternative to NaOCl in the pediatric population.

Antimicrobial Properties and Substantivity of Gentamicin, Amoxicillin and Metronidazole (GAM) Antibiotic Solution with Chitosan as a Root Canal Irrigant - A Preliminary Study.

The combination of gentamicin, amoxicillin, and metronidazole (GAM) solution with chitosan (GAMC) can be used as an alternative intracanal irrigant as it was found to be a potent antibacterial agent.

Antimicrobial Efficacy of Sodium Hypochlorite and Er, Cr: YSGG Laser against E Faecalis Biofilm - A Systematic Review

Background: The complexity of the root canal system makes it difficult to completely disinfect is the leading cause for endodontic retreatment. Sodium hypochlorite (NaOCl) is the traditionally used irrigation solution and has improved its efficacy with various adjuncts. The use of lasers in disinfecting the canal system has come into prominence in recent years. Aim: To assess the efficacy of sodium hypochlorite and Er, Cr: YSGG laser against E faecalis biofilm. Methodology: A systematic search was conducted using a search strategy in PubMed and Google scholar for studies published between 1st January 2011 and 21st March 2022. Hand searching of relevant articles in Institutional library was also performed. Comparative in vitro studies in English language or articles in other languages which had a possibility of translation into English comparing sodium hypochlorite and Er, Cr: YSGG laser for irrigation against E faecalis biofilm were included in the review whereas, studies comparing efficacy against biofilm other than E faecalis, comparison with other laser activated intracanal irrigation, data reported through letter to editors, short communications and conference proceedings were excluded from the review. Results: A preliminary search yielded a total of 67 studies of which seven articles giving 11 estimates were included for qualitative synthesis. Data on colony forming units for both the groups (sodium hypochlorite and Er, Cr: YSGG laser) was extracted. Overall, four studies presented significant difference in the effectiveness of sodium hypochlorite and Er, Cr: YSGG laser whereas three studies presented with no difference among the two groups. Conclusion: Irrigation of root canal by sodium hypochlorite and Er, Cr: YSGG laser activation both prove to be effective in reducing the E faecalis colony forming units. However, it is difficult to provide a conclusive statement on the superiority of Er, Cr: YSGG laser activated irrigation over sodium hypochlorite solution as half of the studies gave contrasting results. Future studies with larger sample size on complex anatomical root structure are recommended.

Effect of operators' proficiency level and patients' related factors on possible complications, using a high frequency polyamide sonic intracanal irrigation device: A prospective clinical cohort study.

Sonic/ultrasonic devices are essential tools in today's endodontics. This prospective trial evaluated for the first time the impact of practitioners' proficiency levels and patient-related factors on complications associated with a high frequency polyamide sonic irrigant activation device. In total 334 patients (females:158, males:176; age:18-95 years) received in the course of their endodontic therapy an intracanal irrigation, using a high frequency polyamide sonic irrigant activation device, by practitioners of different proficiency levels (undergraduate students, general practitioners or endodontists). Intracanal bleeding (yes/no), postoperative pain (0-10 scale), emphysema (yes/no) and polyamide tip fractures (yes/no) were recorded and related to proficiency levels, age, gender, tooth type, smoking-status, systemic conditions affecting healing ability, baseline pain, swelling, fistula, sensitivity to percussion and diagnosis. Intracanal bleeding was associated with patients' age (p<0.05), baseline pain level (OR = 1.14, 95%CI = 0.91-1.22) and baseline swelling (OR = 2.73, 95%CI = 0.14-0.99; p<0.05) but not proficiency level, gender, tooth type, smoking, systemic conditions, baseline fistula or sensitivity to percussion (p>0.05). Postoperative pain development was related to proficiency level (p<0.05) and baseline pain level (p<0.001), with no influence of age, gender, tooth type, smoking, systemic conditions, baseline fistula, swelling or sensitivity to percussion (p>0.05). Emphysema and polyamide tip fractures were not reported. Within the current study's limitations, younger patients with higher baseline pain and swelling, were associated with higher intracanal bleeding. Apart from higher postoperative pain observed with less experienced practitioners, proficiency level had no influence on bleeding, polyamide tip fracture or emphysema, endorsing the high frequency polyamide sonic irrigation device as a safe therapeutic device.

Comparison of Dentinal Tubular Penetration of Intracanal Heated and Preheated Sodium Hypochlorite Through Different Agitation Techniques

IntroductionThe efficacy of sodium hypochlorite (NaOCl) as an intracanal irrigant is widely debated in endodontic therapy. This study aimed to analyze and compare the penetration abilities of different modes of NaOCl application and assess the impact of various agitation strategies on promoting root canal cleanliness. Materials and methodsThis study included 168 single-rooted mandibular premolars that were randomly divided into 8 groups. The 2 modes of application of 5% NaOCl evaluated were intracanal heating and preheating, and the agitation strategies included ultrasonic, sonic, and manual dynamic agitations. The samples were sectioned and observed at a magnification of 1000 × under a scanning electron microscope. ResultsThe analysis of variance test showed a statistically significant difference among the various groups of agitation (P < .05). The post hoc Tukey test confirmed that preheated NaOCl with ultrasonic agitation, intracanal-heated NaOCl with sonic agitation, and manual dynamic agitation had significantly higher debris scores of 1, 4, and 5, respectively, in the apical third of the canal. ConclusionThe results indicated that the combination of intracanal-heated NaOCl and ultrasonic agitation is an effective method for reducing debris in the root canal system. These findings highlight the importance of considering both the mode of application and the agitation strategies when optimizing the use of NaOCl as an intracanal irrigant in endodontic therapy.

Evaluating the Effectiveness of Different Irrigant Activation Techniques in Removing the Smear Layer and Opening the Dentinal Canals: A Scanning Electron Microscopic Study.

Background Although irrigation is one of the most important stages during root canal treatment, it is not possible to guarantee the entry of irrigants to all the areas in the root canal, especially in the apical third. Therefore, the activation of irrigants can stimulate and help the irrigants to access hard-to-reach places within the root canal system. Therefore, this study aimed to evaluate the opening of the dentinal canals with scanning electron microscopy (SEM) using different irrigant activation techniques, such as intracanal heating activation, sonic activation, and ultrasonic activation, along the walls of the root canals in the coronal, middle, and apical thirds. Methodology The study sample consisted of 36 single-canal premolars, which were randomly divided into the following three equal groups according to the method of activation used: group 1 (n = 12), which involved heating activation inside the root canals, group 2 (n = 12), which involved sonic activation, and group 3 (control; n = 12), which involved ultrasonic activation. Afterward, dental crowns were cut to standardize the working length by 18 mm and were then prepared using the ProTaper Next system up to size X2. Moreover, the irrigant was activated for each group separately, and the teeth were extracted and prepared for SEM. Three images were taken for each sample (i.e., coronal third, middle third, and apical third) at ×2,000 magnification. Data were analyzed using the Mann-Whitney U test. Results When studying the removal of the smear layer and the opening of the dentinal canal under ×2,000 magnification, there were no statistically significant differences in the coronal and middle third between the three studied groups. However, statistically significant differences were found in the apical third, where the ultrasonic activation group was the best, followed by the sonic activation group, and the heating activation group. Conclusions All methods of activation were effective in removing the smear layer and opening the dentinal canals with the advantage of ultrasonic activation in the rest of the groups.The intracanal heating irrigant activation proved to be similar to the effectiveness ofsonic activation and close to ultrasonic activation.

Comparison of postoperative pain reduction following laser, ultrasonic activation and conventional needle irrigation after root canal treatment - A randomized clinical trial.

To compare the incidence of postoperative pain and analgesic intake on the administration of passive ultrasonic irrigation (PUI) using IrriSafeTM, laser-activated irrigation (LAI) using Er:Cr: YSGG in comparison to conventional needle irrigation (CNI) in participants diagnosed with symptomatic irreversible pulpitis (SIP). In this randomised double-blinded parallel trial, 75 participants requiring root canal treatment in mandibular first molars diagnosed with SIP were enrolled. A 10 cm visual analogue scale (VAS) was used for pain assessment and patients presented with a preoperative pain score of 5 cm and above were only selected. After biomechanical preparation, the final irrigation protocols varied based on the irrigation protocol employed. In CNI, intracanal irrigation was conducted without agitation using a 31G side vented needle. In PUI, irrigant activation was conducted using IrriSafeTM and in LAI, irrigant activation was conducted using a pulsed Er:Cr: YSGG (2940 nm) laser with radial firing tip (RFT - 2). The pain scores and analgesic intake were assessed postoperatively after obturation at 6, 24 and 48 h. A significant reduction in postoperative pain levels and analgesic intake was seen among all groups at assessed time intervals (p<0.05). Overall, mean postoperative pain scores and analgesic intake were CNI>LAI>PUI (p<0.05) respectively. Participants diagnosed with SIP receiving PUI and LAI showed low and comparable levels of postoperative pain scores. Based on the analgesic intake, PUI showed the least analgesic intake when compared to LAI and CNI respectively. Key words:Endodontics, Root canal irrigants, Postoperative Pain, Passive ultrasonic activation, Laser ultrasonic activation.

The Therapeutic Potential of Plant Extraction in Oral Health - A Systematic Review

Objectives: This study aimed to analyze the therapeutic potential of plant extract against oral microorganisms in published literature. Material and Method: A systematic literature review was performed through electronic databases (Scopus, EBSCO: Dentistry and Oral Science Source) from January 2009 till December 2019 with the search terms (“extract” AND (“ORAL MICROORGANISM” OR “ORAL BACTERIA”) AND (“stem” OR “Bark” OR “Leaf”)). Results: Out of 409 articles, 21 articles met our inclusion criteria that were subjected to data extraction and review. The data disclosed antimicrobial and antibiofilm activity of plant extracts against causative microorganisms of caries, periodontitis, endodontic infection, and fungal infection. Ethanol was the most common solvent used for plant extraction. The antimicrobial test was reported in all studies using different methodologies such as minimum inhibitory concentration (MIC), disk diffusion method, agar well method, intracanal irrigation, and Fractional Inhibitory Concentration Index (FICI) . None of the plant extract tested showed significant toxicity in five studies that conducted toxicity assays. Conclusion: A positive correlation was observed between plant extract and antimicrobial activity against oral microorganisms. In that context, integrating plant extract in oral healthcare products could be an option to enhance effective antimicrobial control. However, further clinical studies are required to provide clinical evidence to support these observations.

A Clinical Comparative Study to Evaluate Anxiety in Children with the Use of Conventional and Camouflaged Syringe.

Background And Objectives: Childhood dental anxiety is one of the most common challenges encountered in pediatric dental operatory, as many children totally avoid the dental examination and treatment due to the same. Visual impact of syringes and needles may hinder the child's cooperation as successful administration of local anesthesia plays a pivotal part in pediatric dental procedures. The aim of this study was to evaluate dental anxiety in children aged 6 to 8 years at the time of administration of local anesthesia, during intracanal irrigation and after treatment with the use of conventional and camouflaged syringe. Methods: In this invivo study, sixty children in the age group of 6 to 8 years were included. Children were divided into 2 groups consisting of 30 children (15 males and 15 females) in each group. In Group A conventional syringe, and in Group B camouflaged syringe were used to administer local anesthesia and intracanal irrigation. Dental anxiety was assessed using two parameters- The Pulse Rate and Facial Image Scale (FIS) score. Pulse rate was measured using pulse oximeter and FIS score was recorded using Facial Image Scale (FIS). Both the parameters were documented in experimental and control groups at the time of administration of local anesthesia, during intracanal irrigation and after treatment. Results: A statistically significant difference was seen in pulse rate between group A and group B at the time of administration of local anesthesia, during intracanal irrigation and after treatment (p=0.00). Intragroup comparison in both group A and group B shows significant difference in all the 3 time intervals showing pulse rate decreases during each time intervals. Facial Image Scale values between the groups at different time intervals shows that the subjects were less anxious in group B compared to group A(p=0.00). Conclusion: The study strongly favors the use of camouflaged syringes to reduce dental fear and anxiety in children and is therefore recommended as an alternative to the use of conventional syringes.

EFFECT OF DIODE LASER DISINFECTION AND THE APICAL BARRIER TECHNIQUE IN AN ENDODONTIC RETREATMENT CASE WITH OPEN APEX: A CASE REPORTS

The primary reason for an endodontic treatment failure is the persistence or regrowth of different species of bacteria within the root canal system. The root development and closure of its apex in a permanent tooth occurs till 3 years after the eruption. Treatment of traumatic injury of teeth during the development period of root resulting in open apex poses an endodontic challenge. Various materials are used for induction of apical closure to produce favourable conditions and to conne the root canal lling within the canal space. This paper describes clinical case of retreatment on premolar with improperly treated root canal with open apex. Multiple visit endodontic retreatment of premolar was performed. Disinfection was performed using Intracanal diode laser irrigation activation followed by placing triple antibiotic paste (TAP) as intra-canal dressing. In the second appointment apical plug of Biodentine against a platelet-rich brin (matrix) was condensed. The present case showed successful periapical healing and resolution of symptoms with the use of calcium silicate based cement with platelet-rich brin as an apical barrier matrix. Procedure is followed carefully using recommended protocols.

Comparison of the antimicrobial efficiency of Curcuma longa and 17% EDTA with 3% sodium hypochlorite against E.faecalis, C.albicans

Aim: To evaluate the antimicrobial efficacy of Curcuma Longa, 17% EDTA, and 3% sodium hypochlorite against E.faecalis, C.Albicans. Materials and Methods: The antimicrobial activity was determined using disc diffusion test. Ethanol extract of turmeric, 3% Sodium hypochlorite, 17% EDTA, absolute ethanol, Enterococcus faecalis cultures, Candida Albican cultures, Mueller Hinton agar. The disc diffusion test was performed in Mueller Hinton agar plates. The plates were incubated at 37°C for 24 hours and zone of inhibition were recorded. Finally, it was statistically analyzed. Results: Ethanol extract of turmeric showed zones of inhibition suggesting that they had anti-microbial properties. Ethanol extract of turmeric showed significantly greater (almost same) zones of inhibition than 2% chlorhexidine. Henceforth research should be directed towards the use as an irrigant in root canal treatment. Conclusion: Under the limitations of this study, it was concluded that Ethanol extract of turmeric has a significant antimicrobial effect against E. faecalis. Microbial inhibition potential of ethanol extract of turmeric observed in this study opens perspectives for its use as an intra-canal irrigant.

Er:YAG laser-induced cavitation can activate irrigation for the removal of intraradicular biofilm

We investigated the biofilm removal effects of laser activated irrigation (LAI) using a pig model, focusing on the impact of the fiber tip position, and used a high-speed camera to observe the occurrence and positioning of the cavitation associated with laser irradiation. A total of 16 roots of deciduous mandibular second premolars from 4 pigs were used. After a pulpectomy, the canals were left open for 2 weeks and sealed for 4 weeks to induce intraradicular biofilm. Root canal irrigation was then performed with Er:YAG laser activation. The fiber tip was inserted at two different positions, i.e., into the root canal in the intracanal LAI group and into the pulp chamber in the coronal LAI group. Intracanal needle irrigation with saline or 5% NaOCl was utilized in the positive control and conventional needle irrigation (CNI) groups. SEM and qPCR were carried out to evaluate treatment efficacy. Statistical analysis was performed using ANOVA and a Tukey–Kramer post-hoc test for qPCR and with a Steel–Dwass test to compare the SEM scores, with α = 0.05. A high-speed camera was used to observe the generation of cavitation bubbles and the movement of the induced bubbles after laser irradiation. The intracanal and coronal LAI groups showed significantly lower amounts of bacteria than either the positive control or CNI groups. There was no significant difference found between the intracanal and coronal LAI groups. SEM images revealed opened dentinal tubules with the destruction of biofilm in both LAI groups. High-speed camera images demonstrated cavitation bubble production inside the root canal after a single pulse irradiation pulse. The generated bubbles moved throughout the entire internal multi-rooted tooth space. Coronal LAI can generate cavitation in the root canal with a simply placed fiber inside the pulp chamber, leading to effective biofilm removal. This method could thus contribute to the future development of endodontic treatments for refractory apical periodontitis caused by intraradicular biofilm.

Investigation of Cellular and Tissue Cytotoxicity of Modified Chlorehexidine as an Intracanal Irrigant; An In-Vitro and Ex-Vivo Study

Investigation of Cellular and Tissue Cytotoxicity of Modified Chlorehexidine as an Intracanal Irrigant; An In-Vitro and Ex-Vivo Study

Assessment of Efficiency of Diode Laser in Root Canal Disinfection: An Original Research.

Introduction:For the complete removal of the intracanal debris and the infectious agents, the intracanal irrigation plays a significant role. Hence, in our study, we assessed the efficiency of diode laser in root canal disinfection.Materials and Methods:We conducted an in vitro study on 45 extracted single noncarious teeth. We equally divided the teeth into controls, Endovac, and diode laser. Later the mean colony-forming units (MCFU) of the Enterococcus faecalis were calculated and compared statistically.Results:We observed that the MCFU in the controls, Endovac, and diode laser was 108/ml, 104/ml, and 0, respectively, after the incubation.Conclusion:We can conclude that diode laser can be successful in irrigation of the root canals.