Some limbic mechanisms regulating intestinal motility were studied in cats by localized electrical or chemical stimulation of the forebrain and the hypothalamus. The intestinal motility was recorded with a transducer and a polygraph through polyethylene cannulae permanently implanted into the proximal and distal segments of the colon. Multipolar electrodes and cannulae were permanently implanted in septal, preoptic and hypothalamic regions. It was found : 1. That low frequency electrical stimulation of certain points elecited intestinal hypomotility, whereas high frequency stimulation of the same areas produced intestinal hypermotility. 2. That cholinergic stimulation of certain areas produced hypermotility whereas the same chemical stimulus induced hypomotility from other areas. It is suggested that cholinergic transmission is involved in the activation of excitatory and inhibitory hypothalamic neurons regulating colonie motility.