Intertidal Research Articles

Non-indigenous species and their realized niche in tidepools along the South-East Pacific coast

Non-indigenous species (NIS) have the potential to colonize and become established in a wide range of coastal habitats. Species with broad environmental tolerances can quickly adapt to local conditions and expand their niches along environmental gradients, and even colonize habitats with extreme abiotic conditions. Here we report and document the distribution of eight marine NIS (four seaweed and four invertebrate species) found in tidepools along a 3000 km latitudinal gradient along the Pacific coast of Chile (18.4°S to 41.9°S). The seaweed NIS Codium fragile, Capreolia implexa, Schottera nicaeensis and Mastocarpus latissimus were mostly distributed towards high latitudes (i.e., more southerly locations), where temperatures in tidepools were low. The invertebrate NIS Anemonia alicemartinae, Ciona robusta, Bugula neritina and Bugulina flabellata were more common towards low latitudes, where high temperatures were registered in the tidepools. Across the intertidal gradient, seaweed NIS were mostly found in pools in the mid and low intertidal zone, while invertebrate NIS occurred mostly in pools from the mid and upper intertidal zones. The realized niche spaces of NIS (based on the Outlying Mean Index, OMI) in the study area were mainly influenced by environmental conditions of temperature and salinity (along the latitudinal and intertidal gradients), while other tidepool characteristics (depth, surface area, exposition, and complexity) only had minor effects. Five of the eight NIS exhibited a realized niche space coinciding with the average tidepool environmental conditions, while marginal niches were occupied by species with affinities for specific temperatures and salinities along the latitudinal and intertidal gradients. Our results indicate that physiological tolerances to environmental factors play a fundamental role in the distribution of seaweed and invertebrate NIS in tidepools along the Chilean coast. This study confirms that tidepools offer suitable conditions for some seaweed and invertebrate NIS, potentially facilitating their invasion into new natural habitats.

Anthropogenic particle abundance and characteristics in seawater and intertidal sediments of the Tonkin Bay Coast (North Vietnam).

Microplastics (MPs, plastic items from 1µm to 5mm in size) are present in all environmental compartments. The evaluation of their concentration, fate, and spatial distribution is still a challenge for the scientific community. This concern is just debuting in developing countries, (i.e., Asia, South America, and Africa). This study deals with the MP contamination in the abiotic marine compartments of Northern Vietnam: seawater and intertidal sediments. Four sites located in the intertidal zone or near the coastline in Tonkin Bay, Vietnam were studied. A total of 16 samples (eight for each compartment) were collected in July 2020 (rainy season) and January 2021 (dry season). Anthropogenic particles (total observed fibers and fragments) were found at levels ranging from 3 to 303 particles/m3 in seawater and from 63 to 955 particles/kg dry weight in sediments. Most of these were fibers less than 300-µm long. Higher levels of seawater at the Nam Dinh site were found in the rainy season compared to the dry one. As the river flow was estimated six times higher during the rainy season than during the dry season, these results suggest the river discharge is a potential source of contamination for the coastal zone. The temporal variability was lower for the sediments than for the seawater, suggesting the long-term integration of the anthropogenic particles in this compartment. A small portion of sorted particles were analyzed by µFTIR (8.35%), and this sub-sample was only composed of fragments. Still, fragments were mostly composed of polypropylene (PP, 82%), polyethylene (PE, 9%), and polystyrene (PS, 9%). The fragment size was similar in the two studied compartments, but it was dependent on polymer types since PS fragments (140 ± 17µm) were smaller than those made of PE (622 ± 123µm) and PP (869 ± 905µm). Future works should investigate the smallest fraction of MP (even nanoplastics) as well as find solutions in order to mitigate MP contamination in the marine environment.

Critical depths of the vertical transition zone between modern infiltration water and fossil groundwater in China

Older groundwater is generally more susceptible to overdraft and also more difficult to recover once contaminated. To understand the prevalence and vulnerability of fossil groundwater infiltrated before Holocene, a global assessment has been conducted recently, but the analysis results for China have been limited by the collection of data. In this study, we combined the groundwater isotope data from the literature databases of CNKI (China national knowledge infrastructure) and SCIE (Science citation expanded) and established the groundwater age dataset of China by virtue of the water samples from 1441 wells. By reconstructing the historical precipitation 3H records of China and using the late-Quaternary atmospheric 14C, the fractions of the modern groundwater that infiltrated after the year 1953 and the fossil groundwater that infiltrated before 12,000 years in the groundwater samples have been calculated based on the straight-forward mixing models. Then we evaluated the extent and depths of the modern and fossil groundwater in the major 16 groundwater-supply areas of China. The results show that the exploitation of deep groundwater mainly occurs in northern China, such as in the Ordos Basin, the North China Plain, and the Hexi corridor, etc. The average critical depth of the vertical transition zone between modern infiltration water and fossil groundwater in China is about 300 m, beneath which more than half of the water samples are dominated by fossil groundwater. Meanwhile, at depth greater than 420 m, the groundwater can rarely be recharged by the modern precipitation. Besides, in China, modern and fossil groundwater, respectively, share 15–45 % and 20–85 % of the total groundwater storage in the uppermost 1000 m of the crust.

Morphology and molecular phylogeny of three Parasonderia species including a new species (Ciliophora, Plagiopylea)

Ciliates of the class Plagiopylea play a vital role in various anaerobic environments as consumers of prokaryotes. Yet, the diversity and phylogeny of this group of ciliates, especially marine representatives, remain poorly known. In this study, three Parasonderia species, viz., Parasonderia elongata spec. nov., and the already known P. cyclostoma and P. vestita, discovered in anaerobic sediments from various intertidal zones in China, were investigated based on their living morphology, infraciliature, and small subunit ribosomal rRNA gene sequences. Parasonderia elongata can be recognized by its larger body size, elongated body shape, oval oral opening, number of oral kineties, and significantly shortened leftmost postbuccal polykineties on the cell surface. Improved diagnosis and redescription of P. cyclostoma is provided for the first time, including data on infraciliature and molecular sequence. Phylogenetic analyses revealed that the three species cluster together and with the sequence of a Chinese population of P. vestita already present in the GenBank database, forming a robust clade.

Pseudoscorpiones (Arachnida) of the Brazilian oceanic islands

Pseudoscorpiones are compose an ancient order of arachnids, occur in a wide variety of terrestrial habitats worldwide, with representatives of various genera occurring exclusively in insular habitats. The primary objective of this study was to determine the composition and distribution of Pseudoscorpiones within the Brazilian equatorial oceanic islands. To determine Pseudoscorpiones communities and distribution in coastal environmental habitats of the Brazilian equatorial oceanic islands (Fernando de Noronha archipelago, Rocas Atoll and St. Peter and St. Paul rocks), was defined three zones along the insular environmental gradient in the Brazilian equatorial oceanic islands. These zones include the Sand Beach (SB) closest to the intertidal zone, the Slope Forest (SF) with a sloping terrain and the Top Forest (TF) farthest from the beach. Collecting points were distributed in each zones (SB, SF, and TF) at distances greater than 1 km, with four samples collected at each point approximately 12.5 m apart from each other. The TF zone is observed only in Fernando de Noronha. In our study we found pseudoscorpions exclusively on the islands of the Fernando de Noronha archipelago. We collected 5 species from the Hesperolpiidae and Syarinidae families. The SF habitats exhibited greater species richness, while the Upper Forest habitats showed greater population abundance. Notably, we recorded pseudoscorpions (Ideoblothrus amazonicus) in bird nests (Sula dactylatra) on Rata Island, suggesting the use of nests as habitat, reproduction, and, possibly, occasional transport to and from other habitats. No pseudoscorpions were found in SB environments. Landscape changes and tourism can threaten this incredible newly discovered community, vital for monitoring environmental changes in this delicate ecosystem.

Detection of Perkinsus sp. in Chionista fluctifraga cultivated in the southeast Gulf of California

Objective. To determine the presence of Perkinsus sp. in the sand clam Chionista fluctifraga cultivated in an intertidal zone of the southeastern Gulf of California, using Ray's thioglycollate fluid medium staining (MFTR) and polymerase chain reaction (PCR). Material and Methods. The prevalence and parasite load of Perkinsus sp. were monthly obtained and correlated with environmental water conditions and clam biometric indicators (n = 540), from May 2018 to September 2019. Results. Presumptive hypnospores of the protozoan were detected in eight months of culture. The prevalence fluctuated from 3.3% to 13.3%, while the average parasite load ranged from 2 to 1286 hypnospores/g of tissue. The intensity of infection varied from negative to light. The prevalence and parasite load were correlated with each other (r=0.61, P˂0.05) but not with the environmental parameters, nor with the biometric indicators of the clam. The PCR test was negative for the MFTR positive cases. Conclusions. Presumptive hypnospores of Perkinsus sp. were detected in C. fluctifraga without clear indications of infection that compromised the clam health in culture. The constant monitoring of Perkinsus sp. in C. fluctifraga is highly recommended to establish possible infections.

Feasibility of ex-situ recruitment and outplanting of Gongolaria nodicaulis (Fucales, Phaeophyceae) for restoration of warm temperate marine forests in Atlantic Morocco

Abstract Brown algal forests provide many ecosystem services but are declining worldwide, prompting a growing number of conservation and restoration efforts. Recent attempts to recover Cystoseira forests are encouraging in the Mediterranean, but whether this is possible in more challenging Atlantic conditions has not yet been investigated. In this study, we assess the feasibility of cultivating Gongolaria nodicaulis by producing ex-situ recruits for reforestation on the Atlantic coast of Morocco. Embryos of G. nodicaulis were cultured on clay tiles for 26 days under controlled conditions without water motion, reaching an average length of 3 mm before being outplanted on the intertidal zone. The transplants were monitored for six months. Recruits experienced high loss rates, approximately 47 % of zygotes within 7 days in culture and 75 % of outplanted seedlings within 10 days in the field. The latter might result from natural self-thinning (competition), but our observations suggested that it was also largely due to the weak attachment of recruits to the tiles resulting from cultivation under calm conditions. Six months after the transplants, the surviving individuals reached the size of wild adult algae (average length of 13.5 cm), and some even became reproductive. Globally, survival rates, growth, physiological condition and fertility in this restoration pilot suggest that ex-situ recruitment and outplanting of G. nodicaulis is a promising approach to recover forests in Atlantic Morocco.

Groundwater, salt and contaminant transport in a coastal aquifer system with a low‐permeability layer in the intertidal zone

AbstractLow‐permeability layer (LPL), formed by natural deposit or artificial reclamation and commonly found below the intertidal zone of coastal groundwater system, can retard the ingress of seawater and contaminants, and shorten the travel time of the land‐sourced contaminant to the marine environment compared with a homogenous sandy coastal aquifer. However, there is limited understanding on how an intertidal LPL, a condition occurred in a coastal aquifer at Moreton Bay, Australia, influences the groundwater and contaminant transport across the shallow beach aquifer system. We characterized the aquifer hydrological parameters, monitored the in situ groundwater heads, and constructed a 2‐D numerical model to analyses the cross‐shore hydrological processes in this stratified system. The calibrated model suggests that in the lower aquifer, the inland‐source fresh groundwater flowed horizontally towards the sea, upwelled along the freshwater–saltwater interface, and exited the aquifer at the shore below the LPL. Whereas in the upper aquifer, the tidally driven seawater circulation formed a barrier that prevented fresh groundwater from horizontal transport and discharge to the beach above the LPL, thereby directing its leakage to the lower aquifer. A contaminant represented by a conservative tracer was ‘released’ the upper aquifer in the model and results showed that the spreading extent of the contaminant plume, the maximum rate of contaminant discharge to the ocean, and its plume length decreased compared with a simulation case in a homogenous sandy aquifer. Sensitivity analysis was also conducted to investigate the characteristics of the LPL, including its continuity and hydraulic conductivity, which were found to vary along the beach at Moreton Bay. The result shows that with a lower hydraulic conductivity and continuous layer of LPL reduced the groundwater exchange and contaminant transport between upper and lower aquifer. The findings from the combined field and modelling investigations on the impact of an intertidal LPL on coastal aquifer systems highlight its significant implications to alter the groundwater and mass transport across the land–ocean interface.

Adapting Methods For Bed Level Assessment In And Around Submerged Vegetation

Coastal vegetation has the capacity to reduce flow and orbital velocities near the bed and stabilize the sediment with its root network. As a result it has an effect on sediment dynamics which gains increasing attention as ecosystem service in coastal protection. To quantify and predict this ecosystem service, laboratory experiments with live or artificial vegetation are often conducted. During these experiments the assessment of bed level change is challenged by the presence of the vegetation. Standard optical and acoustic measurement techniques cannot obtain data below vegetation canopies. Especially for submerged flexible vegetation like seagrass, this challenge is aggravated for airborne methods that require flume drainage (e.g. terrestrial laser scanning) (Follett and Nepf, 2012). Flexible blades will spread on the ground during drainage, potentially covering meadow edges and thus excluding areas of interest from the bed level analysis. Moreover, live aquatic vegetation may be stressed by air exposure, if the facility is drained for bed level measurements. This potentially leads to different, non-natural behaviour in consecutive experiments. And finally, draining and refilling the facility is time consuming, especially as it needs to be done very carefully as not to disturb any generated bedforms. This time aspect hampers the collection of time series and thus the assessment of the development of bed level changes with airborne methods. Underwater technology like sonar and echo sounder avoid shading of areas by spread-out vegetation, but are equally not capable of obtaining data below vegetation canopies. Moreover, instruments that can obtain spatially resolved data underwater often require a minimum water depth which may exceed the water depth relevant for experiments with vegetation. In intertidal areas (e.g. salt marshes) the challenge of obtaining bed level data below vegetation canopies is overcome by the use of a sediment-erosion-bar (SEB) (Cahoon et al., 2002). For this method a horizontal bar is installed at a fixed height above the ground and the distance between this bar and the ground is measured at defined locations along the bar and set time intervals to obtain information on the bed level change. SEBs have successfully been adapted to laboratory settings in the past (Spencer et al., 2016), but still required flume drainage. We applied this method in an undrained flume to assess sediment dynamics in and around an artificial seagrass meadow. Additionally, we tested underwater photogrammetry to obtain 3D-spatial information (e.g., 3D models) on bed level and bedforms at and near the vegetation edges. Photogrammetry has been successfully applied to obtain bedform information in the presence of seagrass stands, but to date still required the drainage of the facility (Meysick et al., 2022).

Turning The Tide: Live-Bed Scale Experiments Of Bar-Dominated Estuaries And Effects Of Dredging On Intertidal Habitat

Many large sand-dominated estuaries and tidal basins have complex channel and bar patterns, wherein a continuous deep channel suitable for shipping is rarely naturally present, which requires dredging. The intertidal area has important, often protected habitats for macro-benthic species, wader birds and other species. The question is to what degree channel and shoal dimensions and the amount of intertidal area are affected by dredging and disposal for access to ports. While numerical modelling is conducted for these systems with some success, complementary scale experiments with live beds have challenged the fields of coastal engineering and coastal morphodynamics for over a century. Compared to scale models for rivers, the scale issues in tidal experiments are more complicated and very few attempts have been pursued. For example, Reynolds (1889) conducted the first scale experiments of an estuary by a periodic sea level fluctuation, but found that even fine sand was hard to mobilize, while the bed surface was dominated by ripples rather than bars. Similarly, Tambroni et al. (2005) carefully scaled sediment mobility in a converging channel and obtained large-scale morphological patterns with gentle bars nearly overwhelmed by ripples. Stefanon et al. (2010) present a careful attempt at erosive tidal channel network development in a scale model with low-density sediment, which showed unexplained, over-large scour holes at channel junctions. The key issues for tidal experiments are to obtain sufficient sediment mobility (expressed as Shields or Rouse number) and to avoid over-large ripples and scours, and to this end classic river scale modelling is inadequate. Here we assess whether our recent innovation solves these scale issues. The objective of this abstract is to elucidate the scaling of experiments that enable study of morphodynamic estuaries in many aspects including effects of sediment management by dredging.

Structure and composition of rehabilitated mangrove in Lubuk Kertang Village, North Sumatra, Indonesia

Mangroves are generally distributed in tidal zones along tropical and subtropical coasts and are known as woody halophytes. Mangroves are one of the most productive and efficient long-term natural carbon sinks. Deforestation and mangrove degradation produced substantial carbon emissions that account for the majority and salt marshes emissions. Mangroves play an important role in protecting against storms and sea level rise, preventing coastline erosion, regulating coastal water quality, providing habitat for commercial interests. This study was conducted to measure the diversity, structure and composition of forests in the rehabilitation of abandoned pond mangrove forests in Lubuk Kertang Village, West Brandan District, Langkat Regency, North Sumatra. The method used was to conduct a census in a 1.6 ha restoration forest by measuring the overall diameter, height and species found. Fourteen species were found, namely Aegiceras corniculatum, Avicennia marina, Acrostichum aureum, Bruguiera gymnorrhiza, Ceriops tags, Exoecaria agallocha, Finlaysonia maritimea, Nypa fruticans Rhizophora apiculata, Rhizophora mucronata, Rhizophora stylosa, Sonneratia alba, Scyphiphora hydrophyllacea, Sesuvium portulacastrum.

On the conditions for the occurrence of crystal avalanches during alloy solidification

Experimental studies on the solidification of ammonium-chloride-water alloys in relatively large containments reveal conditions that lead to the formation of numerous crystal avalanches. Columnar segments that occasionally slide downwards along a vertical mushy zone further fragmentate and so crystal multiplication occurs. As a condition for this phenomenon solidification-induced solutal buoyancy that leads to a rising interdendritic flow was identified for the present case. The interaction with sedimentation-induced downward flow ahead of a vertical columnar region results in a redirection of the interdendritic flow and thus, to local conditions that slow down further solidification or even lead to remelting. Gravity is then pulling loose segments downwards. In larger containment, the flow in the bulk melt is generally unsteady and even turbulent. Thus, the outlined flow-solidification/melting interplay happens frequently at numerous positions but in a stochastic manner.

Macrozoobenthos Community Structure at Teluk Marunggai Beach, Nagari Sungai Nyalo, Pesisir Selatan District, West Sumatera Province

Teluk Marunggai Beach, Nagari Sungai Nyalo, is one of the new tourist areas in Pesisir Selatan Regency which, over time, will begin to be in demand by tourists, which will have an impact on the life of the biota on the beach, one of which is macrozoobenthos. This research was conducted in March 2023 in the Intertidal Zone of Teluk Marunggai Beach, Sungai Nyalo Village, Koto Ampek Tarusan District, Pesisir Selatan Regency, West Sumatra Province. The purpose of this study was to determine the type and abundance of macrozoobenthos species in the coastal waters of Marunggai Bay and to determine the diversity, uniformity, dominance and distribution patterns of macrozoobenthos in the coastal waters of Marunggai Bay. The research method used is the survey method, where the sampling using the quadrant transect method is carried out at three stations. The research results found 40 species of macrozoobenthos. The most common species found at all stations were Turbo cinereus from the Turbinidae family and the Gastropod class. The macrozoobenthos abundance values ​​obtained ranged from 6.44–6.67 ind/m2. Species diversity index values ​​ranged from 3.06–3.52, namely high. Uniformity index values ​​ranged from 3.02 to 3.14, high uniformity, dominance index values ​​ranged from 0.10 to 0.15; no species dominated, and the value of the distribution pattern ranged from 2.959 to 2.962 with a group distribution pattern

Morphological and Molecular Identification of Ulva spp. (Ulvophyceae; Chlorophyta) from Algarrobo Bay, Chile: Understanding the Composition of Green Tides.

Green algae blooms of the genus Ulva are occurring globally and are primarily attributed to anthropogenic factors. At Los Tubos beach in Algarrobo Bay along the central Chilean coast, there have been blooms of these algae that persist almost year-round over the past 20 years, leading to environmental, economic, and social issues that affect the local government and communities. The objective of this study was to characterize the species that form these green tides based on a combination of ecological, morpho-anatomical, and molecular information. For this purpose, seasonal surveys of beached algal fronds were conducted between 2021 and 2022. Subsequently, the sampled algae were analyzed morphologically and phylogenetically using the molecular markers ITS1 and tufA, allowing for the identification of at least five taxa. Of these five taxa, three (U. stenophylloides, U. uncialis, U. australis) have laminar, foliose, and distromatic morphology, while the other two (U. compressa, U. aragoensis) have tubular, filamentous, and monostromatic fronds. Intertidal surveys showed that U. stenophylloides showed the highest relative coverage throughout the seasons and all intertidal levels, followed by U. uncialis. Therefore, we can establish that the green tides on the coast of Algarrobo in Chile are multispecific, with differences in relative abundance during different seasons and across the intertidal zone, opening opportunities for diverse future studies, ranging from ecology to algal biotechnology.

Saccharina sporophytes settling on kelp cultivation rafts come from wild intertidal populations and not from the farmed cultivars

Abstract Wild-type sporophytes of Saccharina japonica have been recurrently observed on the infrastructures of kelp farming rafts in China’s northern production region (Liaoning and Shandong provinces). However, there are few detailed reports delineating their origin, genetic attributes and affiliations with farmed populations. Using microsatellite molecular analyses, we furnish a comparative analysis of the populations sampled from these rafts at one of the historical kelp farming regions Rongcheng city (Shandong province), both before and after the “2021–2022 kelp farming disaster”, in conjunction with two field populations from nearby intertidal zones where natural populations prevail. The results show that the wild kelp populations on the kelp farming rafts in 2023 had originated from the wild populations dwelling in intertidal zones several kilometers away, rather than from the artificially cultivated kelp cultivars.

Estoque de Carbono em Marismas

Salt marshes are typical of temperate climate regions that border estuarine margins, covering intertidal areas subject to regular or irregular flooding. Among the many benefits they provide to the ecosystem, their remarkable ability to sequester carbon from the atmosphere and store it in their substrate for periods ranging from centuries to millennia is particularly noteworthy. This carbon, retained in the form of CO2 in coastal and estuarine environments by salt marshes, is known as "blue carbon." This study aimed to investigate the current state of knowledge about carbon stocks in salt marshes through a systematic bibliographic approach. Three databases were selected for the research: Springer, ScienceDirect, and Periódicos Capes. In each of them, the keywords "tidal salt marsh" and "carbon stock" were used, employing quotation marks around each term and the AND operator. It was observed that, given the urgency to develop actions to mitigate the effects of climate change, studies focusing on salt marshes have received increasing attention globally. However, this interest is recent, and knowledge on the subject is still limited, focusing on specific regions of the planet. The complexity of these habitats is a frequently neglected topic, although of great importance. Invasive plants have an impact on the dynamics of the carbon cycle in these areas. Additionally, the heterogeneity of salt marshes is a crucial factor to be considered in studies to avoid errors in carbon inventories. Salt marsh restoration activities are considered appropriate approaches to coastal management, potentially transforming degraded areas into carbon sinks.

Information fusion-based maintenance strategies selection for coastal concrete bridges using recycled fishing nets

Repurposing recycled materials for coastal concrete bridge maintenance addresses both engineering requirements and marine ecosystem preservation. This study explored the application of recycled nylon (RN) and polyethylene (PE) from discarded fishing nets in coastal concrete beam maintenance. Specifically, these fibers were incorporated into the polymer cement mortar (PCM) to produce two repair materials. Then, thirteen unrepaired or preventive/post-repaired concrete beams were exposed to the tidal zone for one or two years, followed by beam bending tests and the establishment of the corresponding parametric Finite element modeling (FEM). As an alternative to traditional heavy-parameter models, a novel lightweight crack segmentation model (LiteSqueezeSeg) was proposed for quantitatively analyzing beam surface crack properties. Lastly, experimental, simulated, and quantitative crack information were fused to comprehensively assess the beam performance. Results showed that both PCM-RN and PCM-PE effectively inhibit chloride ion penetration. The proposed LiteSqueezeSeg model (95.66% accuracy) achieved nearly identical performance to Deeplab v3 + but only with about 1/6 parameters. Fused results revealed that PCM-PE repaired beams exhibited superior mechanical performance but inferior ductility compared to PCM-RN. Moreover, both PCM-PE and PCM-RN repaired beams demonstrated that preventive repairs achieved more balanced performance than post repairs. Consequently, utilizing PCM-RN for preventive repair emerged as an optimal candidate. This study significantly contributes to the utilization of waste fishing nets in coastal concrete structure maintenance.

A new species of marine algae from the Mexican Atlantic based on morphology and molecular data: Gelidium rodrigueziae sp. nov. (Gelidiaceae, Rhodophyta)

Abstract The algae of the genus Gelidium can be found as frequent and abundant inhabitants in the rocky intertidal zone of the Mexican Atlantic. Six species of this genus have been reported in this region, but only Gelidium americanum has been confirmed with morphological and molecular tools, despite how important both tools have proven to be for the group. This research aimed to evaluate the Mexican specimens previously identified as Gelidium through morphological and molecular approaches, using rbcL and COI-5P markers. This study examined 20 specimens found living attached to the shell of a gastropod Stramonita rustica in the mid-high intertidal zone of the Mexican Atlantic and ten specimens found living on rocks. Phylogenetic analyses resolved these specimens to be an independent species closely related to G. gonzalezii, G. yangmeikengense, and G. indonesianum. Genetic distances and morphological information confirmed this. Gelidium rodrigueziae sp. nov. is described as a new species. It is distinguished from its related species by having a simpler branching pattern, a smaller number and size of medullary cells, and the smaller quantity and distribution of restricted internal rhizoid filaments around the medulla.

Morphodynamics of macrotidal channels in Korean tidal flats: implications for the role of monsoon precipitation and the stratigraphic architecture of tidal point bars

Abstract Tidal channels exert a crucial control on sediment transport and drive geomorphic changes in the tidal environment. Despite their ubiquitous occurrence, long-term morphodynamics and processes driving the morphologic changes remain less well understood than fluvial counterparts. Spanning from straight to dendritic, Korean tidal channels become more sinuous and densely populated with elevation due to higher mud content. Mutually evasive current patterns resulted in a cuspate meander bend, where a flood barb develops at the seaward side of the bend. Multiannual observation revealed that tidal channels migrate from up to 80 m per year in the lower intertidal zone of open-coast sandy tidal flats to nearly stationary for several years in the upper intertidal zone of protected muddy tidal flats. Migration rates are temporarily pronounced during the summer monsoon, when heavy rainfall-induced surface runoff intensifies ebb tidal asymmetry and promotes headward erosion. Meander bends are mostly landward-skewed and shift downstream, implying that ebb currents primarily drive the long-term channel morphodynamics. Tidal point bars commonly display ebbward dipping, inclined heterolithic stratification (IHS) dominated by bedforms formed by subordinate flood tidal currents. An overall ebb dominance and mutually evasive current patterns account for the counterintuitive stratigraphic architecture of point bars in Korean tidal channels.

Mitochondrial responses to constant and cyclic hypoxia depend on the oxidized fuel in a hypoxia-tolerant marine bivalve Crassostrea gigas

Sessile benthic organisms like oysters inhabit the intertidal zone, subject to alternating hypoxia and reoxygenation (H/R) episodes during tidal movements, impacting respiratory chain activities and metabolome compositions. We investigated the effects of constant severe hypoxia (90 min at ~ 0% O2 ) followed by 10 min reoxygenation, and cyclic hypoxia (5 cycles of 15 min at ~ 0% O2 and 10 min reoxygenation) on isolated mitochondria from the gill and the digestive gland of Crassostrea gigas respiring on pyruvate, palmitate, or succinate. Constant hypoxia suppressed oxidative phosphorylation (OXPHOS), particularly during Complex I-linked substrates oxidation. It had no effect on mitochondrial reactive oxygen species (ROS) efflux but increased fractional electron leak (FEL). In mitochondria oxidizing Complex I substrates, exposure to cyclic hypoxia prompted a significant drop after the first H/R cycle. In contrast, succinate-driven respiration only showed significant decline after the third to fifth H/R cycle. ROS efflux saw little change during cyclic hypoxia regardless of the oxidized substrate, but Complex I-driven FEL tended to increase with each subsequent H/R cycle. These observations suggest that succinate may serve as a beneficial stress fuel under H/R conditions, aiding in the post-hypoxic recovery of oysters by reducing oxidative stress and facilitating rapid ATP re-synthesis. The impacts of constant and cyclic hypoxia of similar duration on mitochondrial respiration and oxidative lesions in the proteins were comparable indicating that the mitochondrial damage is mostly determined by the lack of oxygen and mitochondrial depolarization. The ROS efflux in the mitochondria of oysters was minimally affected by oxygen fluctuations indicating that tight regulation of ROS production may contribute to robust mitochondrial phenotype of oysters and protect against H/R induced stress.