Intertidal Sediments Research Articles

Characterization of uncultivated magnetotactic bacteria from the sediments of Yuehu Lake, China

Marine magnetotactic bacteria were collected from the intertidal sediments of Yuehu Lake (China), where their abundance reached 103–104 ind./cm3. Diverse morphotypes of magnetotactic bacteria were observed, including cocci and oval, vibrio-, spirillum-, rod-, elliptical-, handle- and bar-shaped forms. The magnetococci were the most abundant, and had flagella arranged in parallel within a bundle. The majority of magnetosomes were arranged in one, two or multiple chains, although irregular arrangements were also evident. All the results of high-resolution transmission electron microscopy (HRTEM) analysis show that magnetosome crystals were composed of Fe3O4, and their morphology was specific to particular cell morphotypes. By the 16S rRNA gene sequence analysis, we found fourteen operational taxonomic units (OTUs) which were related to magnetotactic bacteria. Among these, thirteen belonged to the Alphaproteobacteria and one to the Gammaproteobacteria. Compared with known axenic and uncultured marine magnetotactic bacteria, the 16S rRNA gene sequences of most magnetotactic bacteria collected from the Yuehu Lake exhibited sequence identities ranging from 90.1% to 96.2% (<97%). The results indicate that microbial communities containing previously unidentified magnetotactic bacteria occur in the Yuehu Lake.

Distribution of Ciliates in Intertidal Sediments across Geographic Distances: A Molecular View

The estimation of the diversity and geographic distribution of protists in particular ciliates has long been an ongoing debate. We estimated the distribution of ciliates in intertidal sediments with geographic distance ranging from 10cm to 1,000km, using high-throughput DNA sequencing. Higher community similarity, in particular that of rare ciliate taxa, occurred between replicates than between sites. Statistical analyses showed that the community dissimilarities of both abundant and moderately abundant ciliates had correlations with geographic distance at the scales of 50m, 200km and 1000km, indicating a distance-decay relationship. No significant correlation was observed for rare taxa. The analysis of Bray-Curtis similarity showed a higher pairwise community similarity in abundant taxa than in moderately abundant taxa, and that in rare taxa was the lowest. Abundant taxa usually can disperse in a wider range than rare taxa, though their distribution is restricted by distance to some extent. Rare taxa are potentially more sensitive to changing environments, but no distance-decay relationship could be observed. The data indicate contrasting patterns of geographic distribution of the abundant and rare ciliate taxa and a weak distance-decay relationship for relatively abundant taxa at a scale over 50m.

A regression approach to the mapping of bio-physical characteristics of surface sediment using in situ and airborne hyperspectral acquisitions

Remote sensing has been successfully utilized to distinguish and quantify sediment properties in the intertidal environment. Classification approaches of imagery are popular and powerful yet can lead to site- and case-specific results. Such specificity creates challenges for temporal studies. Thus, this paper investigates the use of regression models to quantify sediment properties instead of classifying them. Two regression approaches, namely multiple regression (MR) and support vector regression (SVR), are used in this study for the retrieval of bio-physical variables of intertidal surface sediment of the IJzermonding, a Belgian nature reserve. In the regression analysis, mud content, chlorophyll a concentration, organic matter content, and soil moisture are estimated using radiometric variables of two airborne sensors, namely airborne hyperspectral sensor (AHS) and airborne prism experiment (APEX) and and using field hyperspectral acquisitions by analytical spectral device (ASD). The performance of the two regression approaches is best for the estimation of moisture content. SVR attains the highest accuracy without feature reduction while MR achieves good results when feature reduction is carried out. Sediment property maps are successfully obtained using the models and hyperspectral imagery where SVR used with all bands achieves the best performance. The study also involves the extraction of weights identifying the contribution of each band of the images in the quantification of each sediment property when MR and principal component analysis are used.

A High-Level Fungal Diversity in the Intertidal Sediment of Chinese Seas Presents the Spatial Variation of Community Composition.

The intertidal region is one of the most dynamic environments in the biosphere, which potentially supports vast biodiversity. Fungi have been found to play important roles in marine ecosystems, e.g., as parasites or symbionts of plants and animals, and as decomposers of organic materials. The fungal diversity in intertidal region, however, remains poorly understood. In this study, sediment samples from various intertidal habitats of Chinese seas were collected and investigated for determination of fungal community and spatial distribution. Through ribosomal RNA internal transcribed spacer-2 (ITS2) metabarcoding, a high-level fungal diversity was revealed, as represented by 6,013 OTUs that spanned six phyla, 23 classes, 84 orders and 526 genera. The presence of typical decomposers (e.g., Corollospora in Ascomycota and Lepiota in Basidiomycota) and pathogens (e.g., Olpidium in Chytriomycota, Actinomucor in Zygomycota and unidentified Rozellomycota spp.), and even mycorrhizal fungi (e.g., Glomus in Glomeromycota) indicated a complicated origin of intertidal fungi. Interestingly, a small proportion of sequences were classified to obligate marine fungi (e.g., Corollospora, Lignincola, Remispora, Sigmoidea). Our data also showed that the East China Sea significantly differed from other regions in terms of species richness and community composition, indicating a profound effect of the huge discharge of the Yangtze River. No significant difference in fungal communities was detected, however, among habitat types (i.e., aquaculture, dock, plant, river mouth and tourism). These observations raise further questions on adaptation of these members to environments and the ecological functions they probably perform.

Distribution and source analysis of heavy metals in soils and sediments of Yueqing Bay basin, East China Sea

Concentrations of heavy metals in coastal soils, stream sediments and intertidal sediments of Yueqing Bay basin were analyzed to study their distribution and trace the possible sources. According to various single- and multi-index methods, heavy metal enrichment, especially for Cu, Zn, Cr and Ni in stream sediments, should draw environmental concern. Controlling factors such as inorganic scavengers, organic matter, sample grain size and hydrodynamic conditions were identified to influence the transportation and distribution of metals within coastal soils and sediments. Principal component analysis indicated that most metals in soils and stream sediments originate primarily from natural and anthropogenic sources, respectively. Most metals in intertidal sediments, originating both from natural processes and human activities, tend to be concentrated in fine particles. The exchange of water and sediment between the bay and open waters is strong enough to keep the metals in the tidal flats from rising to very high levels.

Influence of introduced Sonneratia apetala on nutrients and heavy metals in intertidal sediments, South China.

To investigate the influence of Sonneratia apetala on nutrients and heavy metals in intertidal sediments, sediment cores of S. apetala marsh and mudflat in Shenzhen Bay, China were analyzed. The results showed that S. apetala improved sediment nutrient properties due to increased total carbon (TC), total nitrogen (TN), and total sulfur (TS). The levels of heavy metals were higher in S. apetala site than in mudflat, including chromium (Cr), nickel (Ni), copper (Cu), zinc (Zn), arsenic (As), cadmium (Cd), lead (Pb), and mercury (Hg). In S. apetala site, TC, TN, and TS were not positively correlated with Cr, Ni, As, Cd, and Pb, indicating their less important roles in trapping heavy metals. There were positive correlations among Ni, Cu, Zn, and Cd in both sites, suggesting similar anthropogenic source. Levels of As were higher than the probable effect level at both sites, indicating their toxicological importance. The geo-accumulation index and potential ecological risk index revealed higher metal contaminations in S. apetala site, especially for Cd, Hg, and As. Multivariate analysis implied that S. apetala alter the biogeochemical cycle of Cd and Cr to a certain extent. These findings indicate that S. apetala may improve soil nutrient properties and facilitate heavy metal accumulation in intertidal sediments.

Geographic distribution at subspecies resolution level: closely related Rhodopirellula species in European coastal sediments

Members of the marine genus Rhodopirellula are attached living bacteria and studies based on cultured Rhodopirellula strains suggested that three closely related species R. baltica, 'R. europaea' and 'R. islandica' have a limited geographic distribution in Europe. To address this hypothesis, we developed a nested PCR for a single gene copy detection of a partial acetyl CoA synthetase (acsA) from intertidal sediments collected all around Europe. Furthermore, we performed growth experiments in a range of temperature, salinity and light conditions. A combination of Basic Local Alignment Search Tool (BLAST) and Minimum Entropy Decomposition (MED) was used to analyze the sequences with the aim to explore the geographical distribution of the species and subspecies. MED has been mainly used for the analysis of the 16S rRNA gene and here we propose a protocol for the analysis of protein-coding genes taking into account the degeneracy of the codons and a possible overestimation of functional diversity. The high-resolution analysis revealed differences in the intraspecies community structure in different geographic regions. However, we found all three species present in all regions sampled and in agreement with growth experiments we demonstrated that Rhodopirellula species do not have a limited geographic distribution in Europe.

Oncholaimus zhangi sp. nov. (Oncholaimidae, Nematoda) from the intertidal zone of the East China Sea

A new species of free-living marine nematode is described from intertidal sediment of the East China Sea. The new species belongs to the Oncholaimidae family. Oncholaimus zhangi sp. nov. is characterized by males having a large ventral swelling situated just posterior to the middle of the tail, no precloacal papilla, and a row of 6–7 ventral genital setae. The tails of both males and females are similar, being conico-cylindrical in shape. The new species is distinguished from other closely related species by the presence of a prominent ventral swelling at the tail of males and a similar tail shape in both sexes. About 110 valid species in this genus have been recorded to date.

Bait worms: a valuable and important fishery with implications for fisheries and conservation management

AbstractBait is an integral part of coastal life, but is perceived as a low‐value resource as fisheries are data‐limited, locally focussed and largely unregulated even though the ecological impacts of collection are considerable. An empirical assessment of three UK‐based ragworm fisheries combined with an analysis of published literature has produced the first global assessment of polychaete bait fisheries. The five most expensive (retail price per kg) marine species sold on the global fisheries market are polychaetes (Glycera dibranchiata, Diopatra aciculata, Nereis (Alitta) virens, Arenicola defodiens and Marphysa sanguinea). We estimate that 1600 t of N. virens per annum (worth £52 million) are landed in the UK with approximately 121 000 tonnes of polychaetes collected globally valued at £5.9 billion. Using remote closed circuit television (CCTV) cameras to monitor collectors, activity at local sites is considerable with a mean of 3.14 collectors per tide (day and night) at one site and individuals digging for up to 3 h per tide, although intensity differed seasonally and between sites. Collectors removed on average 1.4 kg of N. virens per person per hour, walking a considerable distance across the intertidal sediment to reach areas that were usually already dug. The implications of these human activity and biomass removal levels are explored in the context of fisheries and conservation management. At local, regional and national scales, polychaete bait fisheries are highly valuable, extract significant biomass and have considerable impacts; therefore, they urgently require governance equivalent to other fisheries.

Spatial and halophyte-associated microbial communities in intertidal coastal region of India

ABSTRACTMicrobial communities in intertidal coastal soils respond to a variety of environmental factors related to resources availability, habitat characteristics, and vegetation. These intertidal soils of India are dominated with Salicornia brachiata, Aeluropus lagopoides, and Suaeda maritima halophytes, which play a significant role in carbon sequestration, nutrient cycling, and improving microenvironment. However, the relative contribution of edaphic factors, halophytes, rhizosphere, and bulk sediments on microbial community composition is poorly understood in the intertidal sediments. Here, we sampled rhizosphere and bulk sediments of three dominant halophytes (Salicornia, Aeluropus, and Suaeda) from five geographical locations of intertidal region of Gujarat, India. Sediment microbial community structure was characterized using phospholipid fatty acid (PLFA) profiling. Microbial biomass was significantly influenced by the pH, electrical conductivity, organic carbon, nitrogen, and sodium and potassium concentrations. Multivariate analysis of PLFA profiles had significantly separated the sediment microbial community composition of regional sampling sites, halophytes, rhizosphere, and bulk sediments. Sediments from Suaeda plants were characterized by higher abundance of PLFA biomarkers of Gram-negative, total bacteria, and actinomycetes than other halophytes. Significantly highest abundance of Gram-positive and fungal PLFAs was observed in sediments of Aeluropus and Salicornia, respectively than in those of Suaeda. The rhizospheric sediment had significantly higher abundance of Gram-negative and fungal PLFAs biomarkers compared to bulk sediment. The results of the present study contribute to our understanding of the relative importance of different edaphic and spatial factors and halophyte vegetation on sediment microbial community of intertidal sediments of coastal ecosystem.

Distribution, potential sources and ecological risks of two persistent organic pollutants in the intertidal sediment at the Shuangtaizi Estuary, Bohai Sea of China

Spatial distribution, source apportionment, and potential ecological risks of sixteen polycyclic aromatic hydrocarbons (PAHs) and seven endocrine disrupting compounds (EDCs) in the intertidal sediment at the Shuangtaizi Estuary, Bohai Sea of China were analyzed. Results showed that the total PAH concentrations ranged from 28.79ngg−1 dw to 281.97ngg−1 dw (mean: 115.92ngg−1 dw) and the total EDC concentrations from 0.52ngg−1 dw to 126.73ngg−1 dw (mean: 37.49ngg−1 dw). The distribution pattern for the PAHs was generally different from that of the EDCs possibly due to their distinct sources and n-octanol-/water partition coefficients (KOW). Qualitative and quantitative analytical results showed that PAH sources were mainly from a mixture of pyrogenic and petrogenic contributions. The higher levels at the southeast of Geligang indicated that the EDC pollutants may have mainly originated from the plastic industry and other chemical plants located along the Liao River. Ecological risk assessment revealed that PAHs exhibited low ecotoxicological effects, whereas EDCs, especially 4-tert-octylphenol and bisphenol A, had high ecological hazard to the estuarine biota.

Elemental composition, distribution and control of biogenic silica in the anthropogenically disturbed and pristine zone inter-tidal sediments of Indian Sundarbans mangrove-estuarine complex

Spatial distribution and interrelationship among organic nutrients – silica and carbon – and various lithogenic elements were investigated in the surficial sediments of Matla estuary and Core Zone of Indian Sundarbans Reserve Forest using spatial analysis and multivariate statistics. Biogenic silica (BSi), an important parameter for coastal biogeochemisry, was measured using Si-time alkaline leaching method. BSi concentration ranged from 0.01% to 0.85% with higher concentrations in upstream region of Matla estuary and attenuated values towards the bay, seemingly due to changes in hydrodynamics and land use conditions. Spatial distribution of BSi did not exhibit significant correlation with sediment parameters of organic carbon (OC), elemental composition and clay content. However, it showed significant contrasting trends with total phosphorus (TP) and total silica of human influenced Matla estuary sediments as well as the dissolved silica (DSi) of its surface waters. Anthropogenic influence on sediment geochemistry is discernable with the presence of higher concentrations of organic and inorganic elements in Matla estuary than in Core Zone sediments. Spatial variation trends are often challenging to interpret due to multiple sources of input, varying energy and salinity conditions and constant physical, chemical and biological alterations occurring in the environment. Nonetheless, it is certain that anthropogenic activities have a substantial influence on biogeochemical processes of Sundarbans mangrove-estuarine complex and potentially the coastal ocean.

In situ investigation of the effects of current velocity on sedimentary mussel bed stability

The intertidal sediments of the Wadden Sea is an environment where organisms must endure significant physical stresses. Organisms like the blue mussel (Mytilus edulis), face the difficulty of maintaining their position in an unstable substrate. Previous studies on mussel beds in sediment showed that the substrates mussels use for attachment can differ across a bed. Increased adhesion to shell debris in the sediment resulted in what appeared to be a reinforced bed edge. To quantify the erosion resilience in different substrate use zones of an intertidal mussel bed, three distinct zones in a mussel bed were exposed, in situ, to artificially generated current velocities. Velocities ranged from high (0.7ms−1) to very high (1.43ms−1), covering the upper extremes of current velocities measured on mussel beds and those well beyond normal conditions. Silt content of the sediment was decreased 20% towards the bed edge. Results showed that the resilience of the mussel bed to erosion differed significantly between zones. The zone along the bed edge showed the greatest resilience to erosion remaining intact at current velocities of 1.43ms−1. This was also the zone with the greatest mussel cover (90%) and highest density. Erosion resilience was lower in the zones closer to the bed center. Erosion of mussels was always precluded by erosion of the sediment underlying the mussel bed (undercutting). In the zone with an intermediate mussel cover (77%), undercutting already occurred at a current velocity of 0.7ms−1 and was twice as pronounced as in the site with the lowest mussel cover (53%). In all zones, mussel dislodgement did not occur below current velocities of 1.16ms−1. In zones towards the bed center, complete erosion of the bed occurred at 1.43ms−1. Results suggest that a 10% lower mussel coverage increased undercutting by 50%. The reinforced waveward bed edge proved to be capable of resisting erosive forces likely only exceeded during extreme storms. The presence of such a resilient barrier shields the rest of the bed from erosion allowing mussels in the sheltered zones to invest fewer resources in adhesion. Results show the significance of mussel coverage and adhesion on bed resilience. Any predictive modeling or artificial restoration efforts should account for and facilitate the development of such bed edges to improve a beds chance of survival.

Consistent patterns of variation in macrobenthic assemblages and environmental variables over multiple spatial scales using taxonomic and functional approaches

Spatial variability of environmental factors and macrobenthos, using species and functional groups, was examined over the same scales (100s of cm to >100 km) in intertidal sediments of two transitional water systems. The objectives were to test if functional groups were a good species surrogate and explore the relationship between environmental variables and macrobenthos. Environmental variables, diversity and the multivariate assemblage structure showed the highest variability at the scale of 10s of km. However, abundance was more variable at 10s of m. Consistent patterns were achieved using species and functional groups therefore, these may be a good species surrogate. Total carbon, salinity and silt/clay were the strongest correlated with macrobenthic assemblages. Results are valuable for design and interpretation of future monitoring programs including detection of anthropogenic disturbances in transitional systems and propose improvements in environmental variable sampling to refine the assessment of their relationship with biological data across spatial scales.

The transformation and fate of sub‐Arctic microphytobenthos carbon revealed through 13 C‐labeling

Microphytobenthos (MPB) at higher latitudes has been poorly studied. This study used pulse-chase 13C-labeling to investigate the production, processing, and fate of MPB-derived carbon (MPB-C) in sub-Arctic intertidal sediments over 31 d. Gross primary production (2.1 mmol C m−2 h−1 ± 0.4 mmol C m−2 h−1) was comparable to that reported for temperate regions. Some of the 13C fixed by sub-Arctic MPB was rapidly (within 0.5 d) transferred to deeper sediments (below 2 cm), but most was retained within surface sediments (>70.2% of the 13C present at any time during the study). At any time, MPB accounted for ≥ 49.8% of this 13C. The 13C content of sediment organic carbon declined over time, but > 31% of the 13C fixed within the first tidal cycle remained after 31 d, suggesting that sub-Arctic MPB may contribute to coastal carbon retention during the productive season. Over 21 d, 10.6% of the fixed 13C was removed via DIC fluxes and 0.3% via DOC fluxes from inundated sediment, and 0.6% as CO2 from exposed sediment. The greatest loss of 13C (38.2%) was via unmeasured pathways, including resuspension and/or removal by mobile consumers. The rates of MPB-C production and the relative importance of the pathways for MPB-C loss were similar to that observed for comparable lower latitude sediments, demonstrating that MPB at higher latitudes are not necessarily distinct from MPB at lower latitudes and probably play a similarly important role in ecosystem functioning. Apparently, local environmental conditions are more important than climate differences for determining the processing and fate of MPB-C.

The radius of influence of a combined method of in situ air sparging and soil vapor extraction in the intertidal sediments of Gomso Bay on the west coast of South Korea.

BackgroundIn situ air sparging (IAS) was undertaken at sites in the tidal flats of Mandol and Hajeon, on the west coast of South Korea, to estimate variations in the radius of influence (ROI).ResultsThe Mandol core sample consisted of sand and muddy sand 1.6–3.4 Phi (average 2.3 Phi) and contained water (average 15.10 %). The Hajeon core sample consisted of muddy sand, sandy silt, and muddy sandy gravel 1.31–4.44 Phi (average 3.11 Phi) and contained water (average 19.77 %). These sites differ in their sedimentary and geochemical characteristics. At the Mandol site, no H2S or combustible gas was detected during a 48-h sampling period, except for some volatile organic compounds (0.1–2.0 ppm) at the monitoring well during the initial 30 min. At the soil vapor extraction wells, CO2 and O2 varied by 850 ppm (690–1540 ppm) and 0.5 % (20.4–20.9 %), respectively. At the Hajeon site, CO2 and O2 varied from 580 to 1250 ppm and 20.6 to 20.9 %, respectively, during the 48-h sampling period.ConclusionsAt the Mandol site, an oxygen concentration of 20.6 % was assumed as the effective concentration, and the ROI was estimated to be 128.0 cm. However, at the Hajeon site the ROI was estimated to be 85.7 cm. The smaller effective ROI at the Hajeon site was likely caused by the thin aquifer and thin screens of the sparing well. This estimated ROI show that the remediation effectiveness varies greatly as a heterogeneities and anisotropies in the porous sediments. Besides, injection pressure, flow rate, pulsing or continuous mode, and the range of intrinsic permeability for most important characteristic of sediment (soil) type impacted the ROI. Therefore, the IAS method is more effective at a pervasive air flow sediments such as Mandol, which consists of sand and muddy sand than at a channelized site such as Hajeon.Electronic supplementary materialThe online version of this article (doi:10.1186/s40064-016-3026-3) contains supplementary material, which is available to authorized users.

Polycyclic aromatic hydrocarbons (PAHs) in surface sediments from the intertidal zone of Bohai Bay, Northeast China: Spatial distribution, composition, sources and ecological risk assessment

Polycyclic aromatic hydrocarbons (PAHs) can enter intertidal zones by various pathways and pose potential threats to intertidal ecosystem. We investigated distribution, composition, sources and risk assessment of PAHs in intertidal surface sediments of Bohai Bay. Total PAH concentrations ranged from 37.2ng·g−1 to 206.6ng·g−1, among which high values occurred near Nanpaishuihe River Estuary and Haihe River Estuary. The composition patterns of PAHs were characterized by the predominance of 3-ring and 4-ring PAHs, and acenaphthylene was the most dominant component. Diagnostic ratios and principal component analysis have confirmed that PAH contaminants originated from mixed sources, and the major was local combustion. The mean benzo(a)pyrene equivalent concentration of total PAHs in intertidal sediments was 15.67ng·g−1, which was mostly contributed by seven carcinogenic PAHs. According to ecological risk assessment, negative effects related to acenaphthylene would occur occasionally in partial survey regions of the study.

Two new species of Chromadoridae (Chromadorida: Nematoda) from the East China Sea.

Two new species of Chromadoridae, a family of free-living marine nematodes, are described from intertidal sediments of the East China Sea. Ptycholaimellus pirus sp. nov. is characterized by having a cuticle with six longitudinal rows of double dots and long somatic setae, relatively long cephalic setae, a pear-shaped terminal pharyngeal bulb occupying less than 30% of pharyngeal length, and an elongate conical tail. The new species is distinguished from all related species by the unique character of the cuticle, with six longitudinal rows of horizontal double dots, and the pear-shaped terminal pharyngeal bulb. Hypodontolaimus ventrapophyses sp. nov. is characterized by having a cylindrical body with a slightly expanded anterior end and a conical tail, a homogeneous cuticle with lateral differentiation of two longitudinal rows of larger dots, a well developed pharynx with oval-shaped buccal bulb and terminal bulb, and a large ventral gland. Males have slender, strongly curved spicules and a gubernaculum with a ventral apophysis, and precloacal supplements are absent. The new species differs from all related species in this genus by the structure of the gubernaculum, which has a ventral apophysis.

The balance between nitrogen fixation and denitrification on vegetated and non‐vegetated intertidal sediments

Rates of denitrification (isotope pairing) and nitrogen fixation (acetylene reduction) were simultaneously measured in three temperate, intertidal Zostera muelleri meadows and adjacent non-vegetated tidal flats within Western Port, Australia. Net daily nitrogen fluxes ranged from −276 (net denitrification) to 520 μmol N/m2 d (net nitrogen fixation), and were generally positive, with only two instances of net negative fluxes. The highest fluxes were observed at the sites with the lowest water column nitrate concentrations. No significant differences in net nitrogen fluxes were found between vegetated and non-vegetated sediments (p = 0.213). Nitrogen fixation was generally the dominant process occurring, which was stimulated in the presence of vegetation except at the most marine-influenced site, where nitrogen fixation in non-vegetated sediments was higher. Nitrogen fixation rates in non-vegetated sediments were highly correlated to cyanobacterial cell counts (although no mats were present). Rates were ∼65 μmol N/m2 d at 0 cell counts, suggesting a basal rate driven within the sediment. Additional slurry experiments confirmed significant rates of nitrogen fixation within the sediment, which were stimulated by sucrose and terminated by nitrate (p < 0.05), strongly suggesting sulfate-reducing bacteria contributed to nitrogen fixation. At the bay-wide scale, nitrogen fixation was estimated to contribute ∼430 t N/yr compared to ∼650 t N/yr from catchment and atmospheric inputs and 230 t N/yr lost through denitrification. Sensitivity analysis confirmed that while the loss of seagrass would affect the magnitude of the bay-wide flux of nitrogen on these tidal flats, nitrogen fixation remains the dominant process.

Restoration and source identification of polycyclic aromatic hydrocarbons after the Wu Yi San oil spill, Korea

On January 31, 2014, an oil spill accident occurred in Yeosu, South Korea. A total 800–899kl of oil from the pipeline was spilled into the sea. After the oil spill, the KIOST (Korea Institute of Ocean Science & Technology) researched PAHs (polycyclic aromatic hydrocarbons) in various media, but sedimentary PAHs were not analyzed despite their longer persistency than in other media. Therefore, this study examined PAH levels in intertidal sediments around Gwangyang Bay and identified PAH sources using oil fingerprinting. PAH residual levels showed a dramatic decrease during the four months after the accident and then remained at a relatively constant level. Analysis through regression equations indicate that this study area is likely to be restored to the PAH levels prior to the accident. Furthermore, the source analysis and oil fingerprinting analysis showed that PAH contamination in this study was unlikely to have originated from the spilled oil.