Abstract In Central America, the family Manaosbiidae is recorded only from Panama and Costa Rica. Four species occur in this region: Barrona williamsi Goodnight & Goodnight 1942, Bugabitia triacantha Roewer 1915, Poassa limbata Roewer 1943, and Zygopachylus albomarginis Chamberlin 1925. In this paper, we describe Barrona felgenhaueri new species (Cocle Province, Panama) and Bugabitia akini new species (Cocle Province, Panama) and report a new record for B. williamsi (Cocle Province, Panama). We used SEM to examine the penis morphology of Barrona Goodnight & Goodnight 1942 and the Caribbean species Cranellus montgomeryi Goodnight & Goodnight 1947 and Rhopalocranaus albilineatus Roewer 1932. We compared genital morphology of these species with published descriptions for Manaosbiidae from South America. With respect to genital morphology, we found that the most variable characters were the number and relative sizes of the setae that occur on the lateral margins of the ventral plate. Other features that exhib...